##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2150854
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.497536327430872	31.0	31.0	34.0	26.0	34.0
2	30.546722371671905	31.0	31.0	34.0	26.0	34.0
3	30.678069269229805	31.0	31.0	34.0	26.0	34.0
4	34.233031623717835	37.0	35.0	37.0	30.0	37.0
5	34.16173575705278	37.0	35.0	37.0	30.0	37.0
6	34.178480268767665	37.0	35.0	37.0	30.0	37.0
7	34.04714499449986	37.0	35.0	37.0	30.0	37.0
8	33.99570542677467	37.0	35.0	37.0	30.0	37.0
9	35.56240126015062	39.0	37.0	39.0	30.0	39.0
10	35.46257812013275	39.0	35.0	39.0	30.0	39.0
11	35.433592424218475	39.0	35.0	39.0	30.0	39.0
12	35.36007650914474	39.0	35.0	39.0	30.0	39.0
13	35.32540841916746	39.0	35.0	39.0	30.0	39.0
14	36.45775166515254	40.0	37.0	41.0	30.0	41.0
15	36.42380700875094	40.0	37.0	41.0	30.0	41.0
16	36.41038257361959	40.0	37.0	41.0	30.0	41.0
17	36.42804857977343	40.0	37.0	41.0	30.0	41.0
18	36.39699672781137	40.0	37.0	41.0	30.0	41.0
19	36.419580780471385	40.0	37.0	41.0	30.0	41.0
20	36.3915686513357	40.0	37.0	41.0	30.0	41.0
21	36.30251425712763	40.0	37.0	41.0	29.0	41.0
22	36.22560620107176	40.0	37.0	41.0	29.0	41.0
23	36.1063991326236	40.0	36.0	41.0	29.0	41.0
24	36.02780244498232	40.0	36.0	41.0	29.0	41.0
25	35.753345415355945	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9952217328055539
1101	2	1.1115006314499816
1101	3	1.0492224391816691
1101	4	0.9057357262999943
1101	5	1.0478730957606786
1101	6	1.0559868110705963
1101	7	1.148915594350413
1101	8	1.1314484584413265
1101	9	1.2484773007409729
1101	10	1.26491576347874
1101	11	1.2400270473543529
1101	12	1.330478152960481
1101	13	1.407938426053441
1101	14	1.6269846723624468
1101	15	1.6552824785755291
1101	16	1.6205186739533275
1101	17	1.408359891851049
1101	18	1.516428892535032
1101	19	1.5813725049711849
1101	20	1.6396183186626843
1101	21	1.657838425292958
1101	22	1.7251558460474854
1101	23	1.7858352279673682
1101	24	1.8423590950245483
1101	25	1.7067325250297216
1102	1	0.9272850824834116
1102	2	1.0528306595124555
1102	3	1.0358094237685194
1102	4	0.9886661034766533
1102	5	0.9848313172178678
1102	6	1.043160501437832
1102	7	1.09502715330283
1102	8	1.016468065809093
1102	9	1.14426914140914
1102	10	1.1329093872960883
1102	11	1.1547637873794443
1102	12	1.133107419153987
1102	13	1.1393968869205295
1102	14	1.3632234519174489
1102	15	1.4406427147124887
1102	16	1.373338558915357
1102	17	1.384871053201202
1102	18	1.2856747210448063
1102	19	1.3134661596996864
1102	20	1.3480916108215553
1102	21	1.2972986317309605
1102	22	1.4120986119288474
1102	23	1.4703644996157834
1102	24	1.4471879017934839
1102	25	1.4224911513623866
1103	1	0.7015963859703511
1103	2	0.8331609345305893
1103	3	0.7725748237477994
1103	4	0.6426733789667765
1103	5	0.7058674989669811
1103	6	0.6408730965545075
1103	7	0.7740134196640511
1103	8	0.7761575911754548
1103	9	0.8453978784439187
1103	10	0.9629426565467796
1103	11	0.9357974365212343
1103	12	0.9514400425898089
1103	13	1.0301263929469897
1103	14	1.07801272747664
1103	15	1.0202598305332273
1103	16	1.1409706861903857
1103	17	1.1040152621643244
1103	18	1.1118601539479798
1103	19	1.1147635453582225
1103	20	1.118970655798357
1103	21	1.128560791124059
1103	22	1.1886604823744662
1103	23	1.1221574704792872
1103	24	1.2181837920625185
1103	25	1.1472001893169548
1104	1	0.7458054108216423
1104	2	0.7238729162172852
1104	3	0.6651849030013928
1104	4	0.5272640176779078
1104	5	0.6517985986322756
1104	6	0.5171230315214714
1104	7	0.6117763699094425
1104	8	0.7143081383200709
1104	9	0.7459428552921565
1104	10	0.8986236825079672
1104	11	0.9391253937901993
1104	12	0.8436135333898065
1104	13	0.8161941487002409
1104	14	0.9599539202692426
1104	15	0.9590082480626592
1104	16	0.9529818545305702
1104	17	1.0490879257440895
1104	18	0.9296167743111283
1104	19	0.9219375564225061
1104	20	0.9766532252102849
1104	21	0.938747566005695
1104	22	0.9648562231441105
1104	23	1.0479539084030804
1104	24	1.0471022493054676
1104	25	1.0279252643217234
1105	1	0.5869802391214058
1105	2	0.5806804926766844
1105	3	0.6079104048268427
1105	4	0.562778007356691
1105	5	0.40285500339633984
1105	6	0.4405754010950531
1105	7	0.48353373318850146
1105	8	0.5265093937925442
1105	9	0.6642243048040726
1105	10	0.5242602825368934
1105	11	0.5950376659355854
1105	12	0.617849470390702
1105	13	0.6517913232336241
1105	14	0.6489641859297706
1105	15	0.6816322002357609
1105	16	0.642833669892461
1105	17	0.7094720220579802
1105	18	0.6916723490647598
1105	19	0.556934239648534
1105	20	0.5392463052433882
1105	21	0.6671178699316584
1105	22	0.6637841331435297
1105	23	0.674679182302441
1105	24	0.7553802349943197
1105	25	0.6781197775933165
1106	1	0.8202182188494298
1106	2	0.7688060100365313
1106	3	0.7837207715507084
1106	4	0.7780343112380379
1106	5	0.7448759363447479
1106	6	0.769061016017595
1106	7	0.744830688453618
1106	8	0.7411623406927461
1106	9	0.9202565828914899
1106	10	0.818858858091879
1106	11	0.9134702558305605
1106	12	1.008370302292171
1106	13	0.9221035373178239
1106	14	1.0452255368937173
1106	15	1.074158470246637
1106	16	1.045604725018464
1106	17	1.0751640658861277
1106	18	1.0395763294172937
1106	19	1.0137205141107444
1106	20	1.0288383729464954
1106	21	0.9660568331929795
1106	22	1.039806264616864
1106	23	1.204791414110069
1106	24	1.0198685772580944
1106	25	1.120736375671875
1107	1	0.4055954122943781
1107	2	0.4659393475614806
1107	3	0.3972812379405575
1107	4	0.28459677766867486
1107	5	0.25153116346605486
1107	6	0.2835383095256745
1107	7	0.35072190394102165
1107	8	0.43669722449235593
1107	9	0.35167819075662266
1107	10	0.36536518691790576
1107	11	0.49923559684639685
1107	12	0.5364039081652479
1107	13	0.5635539573865387
1107	14	0.6916147353765041
1107	15	0.6905608723256051
1107	16	0.6116008550086178
1107	17	0.6995087379216329
1107	18	0.6676545487654053
1107	19	0.7905910165212546
1107	20	0.6869533873685754
1107	21	0.6783269861578987
1107	22	0.7985216108158326
1107	23	0.769909935863673
1107	24	0.6798880523040154
1107	25	0.6031802166862548
1108	1	0.39142953763289157
1108	2	0.4424611023661953
1108	3	0.36759858870541606
1108	4	0.3134414736800508
1108	5	0.25135850374415014
1108	6	0.1758457653421246
1108	7	0.20016857390960752
1108	8	0.32736528219675876
1108	9	0.33169852379499076
1108	10	0.30732983495421706
1108	11	0.3592890820153656
1108	12	0.4661124609336582
1108	13	0.35698714177989643
1108	14	0.4405759176829349
1108	15	0.38277950034235886
1108	16	0.4811379979394843
1108	17	0.4606796252050316
1108	18	0.49990725671857916
1108	19	0.45106560109253735
1108	20	0.5183723510790159
1108	21	0.5324928388594827
1108	22	0.49303166300627765
1108	23	0.6895165681563853
1108	24	0.5712448274047048
1108	25	0.570269827996917
1109	1	0.2966657791143419
1109	2	0.30980460112914443
1109	3	0.2890971505792912
1109	4	0.3187362807475793
1109	5	0.25690210909224476
1109	6	0.2482764726920692
1109	7	0.29283797996456684
1109	8	0.31424444655794304
1109	9	0.3586772742598967
1109	10	0.3985465262165562
1109	11	0.3634812144003021
1109	12	0.2908008083506175
1109	13	0.41364178832287024
1109	14	0.3916827053148424
1109	15	0.4894796881318939
1109	16	0.4598608674141573
1109	17	0.47124252274619494
1109	18	0.6049294842216284
1109	19	0.4818410959295534
1109	20	0.5817521970648798
1109	21	0.5441090205379453
1109	22	0.4772730910222833
1109	23	0.4420311796725045
1109	24	0.5089265266081995
1109	25	0.5032428154112978
1110	1	0.18502215503626473
1110	2	0.21261765735141935
1110	3	0.15669900987111873
1110	4	0.26231004994442486
1110	5	0.05097134304406836
1110	6	0.058250952428984704
1110	7	0.1798195217212637
1110	8	0.22615777672727688
1110	9	0.3253383348642842
1110	10	0.3739905975709874
1110	11	0.3830156436082035
1110	12	0.2855036460433027
1110	13	0.26542354491207476
1110	14	0.26982506778610116
1110	15	0.19596404608506646
1110	16	0.16793971573580535
1110	17	0.10672264879680426
1110	18	0.15771010190069745
1110	19	0.21171196009623117
1110	20	0.24081242462676755
1110	21	0.3035306853409594
1110	22	0.27576110527193265
1110	23	0.3532245975618693
1110	24	0.3449848972091729
1110	25	0.34652645065185084
1111	1	-0.27316106438955856
1111	2	-0.2669405510573597
1111	3	-0.3384860987186755
1111	4	-0.3443995167858418
1111	5	-0.3372334942923274
1111	6	-0.41392234913102754
1111	7	-0.36638937471278865
1111	8	-0.3513327406979698
1111	9	-0.3117588229907895
1111	10	-0.3106963475949982
1111	11	-0.270747880591486
1111	12	-0.3773258597168123
1111	13	-0.29638925633199875
1111	14	-0.3102460580273032
1111	15	-0.4553830792937674
1111	16	-0.4048415004991739
1111	17	-0.49483188351919694
1111	18	-0.46657923746793273
1111	19	-0.526497804281135
1111	20	-0.5108200770758344
1111	21	-0.48977003176025846
1111	22	-0.3425778614246653
1111	23	-0.4163298177416124
1111	24	-0.38017065893613733
1111	25	-0.4377091142301879
1112	1	-0.4858199930558591
1112	2	-0.6367998039055429
1112	3	-0.5159458773101626
1112	4	-0.4966513873291305
1112	5	-0.4593130354104318
1112	6	-0.4697409267778738
1112	7	-0.4782558914838475
1112	8	-0.3859066471176078
1112	9	-0.4404681396584209
1112	10	-0.5681706840545289
1112	11	-0.4529704602686593
1112	12	-0.42803130262581845
1112	13	-0.4688609194830633
1112	14	-0.5055756490721848
1112	15	-0.502738608421204
1112	16	-0.616639863725041
1112	17	-0.4740174582201746
1112	18	-0.6147288709237131
1112	19	-0.5393207076398241
1112	20	-0.5445952286552966
1112	21	-0.528520053651512
1112	22	-0.6264621013551661
1112	23	-0.5782329720101274
1112	24	-0.6686197588634215
1112	25	-0.7556455496805654
1113	1	-0.4468188822647292
1113	2	-0.41533249480310275
1113	3	-0.409657202297641
1113	4	-0.3893063717320757
1113	5	-0.4418126300563685
1113	6	-0.36362165346275077
1113	7	-0.5678826995444624
1113	8	-0.6349682827151923
1113	9	-0.6309736585237147
1113	10	-0.6776368509055217
1113	11	-0.5583879265516742
1113	12	-0.43353443776723566
1113	13	-0.4908262695758765
1113	14	-0.6183681392529365
1113	15	-0.6513123671302665
1113	16	-0.4938151630215444
1113	17	-0.7065333880297899
1113	18	-0.7739216960358206
1113	19	-0.794316242695082
1113	20	-0.741414590785304
1113	21	-0.8163420076274903
1113	22	-0.8753870055827022
1113	23	-0.7124063764276443
1113	24	-0.7763725485463766
1113	25	-0.8148018324265252
1114	1	-0.8701770424705622
1114	2	-1.4639928656732124
1114	3	-0.9435799954658073
1114	4	-0.8693193122732552
1114	5	-1.0307044115007145
1114	6	-0.8670646384326659
1114	7	-0.7669419898724783
1114	8	-0.8820753052720605
1114	9	-0.8586342379340337
1114	10	-0.9890127881280151
1114	11	-1.0072996505162592
1114	12	-1.0248416257752027
1114	13	-0.9533199684852462
1114	14	-1.2175651213585255
1114	15	-1.292263448570047
1114	16	-1.2196252588533625
1114	17	-1.2347422486406927
1114	18	-1.2789265918969193
1114	19	-1.156131426355472
1114	20	-1.2618210094042155
1114	21	-1.3059397183808485
1114	22	-1.2673024818899137
1114	23	-1.5013412469738938
1114	24	-1.515724637608237
1114	25	-1.4947549039751493
1115	1	-1.109160933561224
1115	2	-1.4482707739661755
1115	3	-1.38404850060639
1115	4	-1.1951267544847113
1115	5	-1.3438535944878964
1115	6	-1.3774408251186472
1115	7	-1.4697353667394353
1115	8	-1.4567810952806255
1115	9	-1.9378837924534196
1115	10	-1.8118494874678461
1115	11	-1.7393931201974127
1115	12	-1.7081061517480336
1115	13	-2.078874168396567
1115	14	-1.9947059758550623
1115	15	-2.0209251339326713
1115	16	-2.0724175340681157
1115	17	-2.1385776507850593
1115	18	-2.030268679693087
1115	19	-2.026466962075169
1115	20	-2.0412429724933574
1115	21	-2.083088146736273
1115	22	-2.2932187122564045
1115	23	-2.2742707605118184
1115	24	-2.2549462101616697
1115	25	-2.182281997734009
1116	1	-1.3232844762968945
1116	2	-1.3824608486533343
1116	3	-1.3658654594974173
1116	4	-1.412360469965222
1116	5	-1.442354726714619
1116	6	-1.3512637157887255
1116	7	-1.625090493346434
1116	8	-1.585993581910408
1116	9	-1.8034785588465354
1116	10	-1.7163689229208572
1116	11	-1.8032241843746135
1116	12	-1.8700191489665414
1116	13	-1.7122270953707996
1116	14	-1.9680856455563003
1116	15	-2.048197712845756
1116	16	-2.152783363714171
1116	17	-2.2674537561874573
1116	18	-2.1858492297659993
1116	19	-2.2267771404937236
1116	20	-2.102361176597377
1116	21	-2.1335950521220184
1116	22	-2.279011695729878
1116	23	-2.4833737505146445
1116	24	-2.524134086283702
1116	25	-2.6985473379305063
1201	1	0.8284663775869454
1201	2	1.0007998110603253
1201	3	0.9649871375214083
1201	4	0.8867328683751126
1201	5	0.8930781191986199
1201	6	0.8639668080622585
1201	7	0.8523594061662934
1201	8	0.9219494953329743
1201	9	1.0362503632577713
1201	10	1.0387150774781375
1201	11	1.1032283670794598
1201	12	1.0250880425148736
1201	13	1.1855758419369948
1201	14	1.3063200753693138
1201	15	1.3777639168589033
1201	16	1.3607096296937158
1201	17	1.3886853352732587
1201	18	1.3477521438129543
1201	19	1.3896353354264548
1201	20	1.359183144733663
1201	21	1.4012929389155673
1201	22	1.4238085033751346
1201	23	1.4093584896018854
1201	24	1.4830254674326326
1201	25	1.5353098011979753
1202	1	0.7427558635936222
1202	2	0.7985424360646896
1202	3	0.7769581698844839
1202	4	0.7766073964743967
1202	5	0.8393157557945372
1202	6	0.8773543676282856
1202	7	0.8470550819223774
1202	8	0.8632157030568166
1202	9	1.0180556701648271
1202	10	0.9927580582883451
1202	11	1.0605498411746055
1202	12	0.9964800856458709
1202	13	0.9966443260720936
1202	14	1.202624601933259
1202	15	1.288551066140954
1202	16	1.2634826577071436
1202	17	1.2246145510033273
1202	18	1.1937418785823937
1202	19	1.243758765951526
1202	20	1.2221711102789925
1202	21	1.2428012610607126
1202	22	1.3047936249608654
1202	23	1.3555705372575773
1202	24	1.341418424591005
1202	25	1.4321705882755182
1203	1	0.6511517411853305
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2211	8	-0.16300669132883172
2211	9	-0.21650141897563913
2211	10	-0.33522386555981143
2211	11	-0.2988262418637362
2211	12	-0.35366499309402855
2211	13	-0.3644249560851378
2211	14	-0.47952433877379264
2211	15	-0.4761984168808766
2211	16	-0.4090162756624025
2211	17	-0.28820050160293675
2211	18	-0.3789403697971636
2211	19	-0.3207773343190965
2211	20	-0.4316749636615853
2211	21	-0.4879182248564575
2211	22	-0.5065006080954788
2211	23	-0.5518372513828211
2211	24	-0.4715802452136373
2211	25	-0.3390921869732182
2212	1	-0.44799101840985855
2212	2	-0.4957989704108847
2212	3	-0.5462687768676737
2212	4	-0.4344984290982339
2212	5	-0.3855955773299016
2212	6	-0.4678168735663064
2212	7	-0.5979171189826786
2212	8	-0.5860231514418999
2212	9	-0.5296201458062271
2212	10	-0.6397968469512918
2212	11	-0.6898755752898964
2212	12	-0.8286423852721185
2212	13	-0.6354742115726708
2212	14	-0.8234425367908074
2212	15	-0.8422159622143681
2212	16	-0.8869600568832823
2212	17	-0.8924289867765793
2212	18	-0.8712599304511386
2212	19	-0.7994433967615748
2212	20	-0.8745761150773745
2212	21	-0.804394382793987
2212	22	-0.8129358729852569
2212	23	-0.7972641838092116
2212	24	-0.8694764868707381
2212	25	-0.7566963341474207
2213	1	-0.7843288092942444
2213	2	-0.7078052470551945
2213	3	-0.5964051816585645
2213	4	-0.6722758423915849
2213	5	-0.6699580903176781
2213	6	-0.7142678716857915
2213	7	-0.6672924498206072
2213	8	-0.6788986117314479
2213	9	-0.8124853870711846
2213	10	-0.7463433991530835
2213	11	-0.8494320027386308
2213	12	-0.892936712911883
2213	13	-0.9551478904860886
2213	14	-1.1218204271364058
2213	15	-1.2105585890542798
2213	16	-1.128007529029425
2213	17	-1.099448927838914
2213	18	-1.1894625253884286
2213	19	-1.2885388117804197
2213	20	-1.1435631563502042
2213	21	-1.1445485481001825
2213	22	-1.0771610968923042
2213	23	-1.1680247884139163
2213	24	-1.2440959031045153
2213	25	-1.1363250818515525
2214	1	-1.0329629265992253
2214	2	-1.53471552858867
2214	3	-1.0401238599339102
2214	4	-0.916853834784682
2214	5	-1.0093195989086325
2214	6	-1.054468395747108
2214	7	-0.8574875246280484
2214	8	-1.0689893520567608
2214	9	-1.3498918645165716
2214	10	-1.316657433080941
2214	11	-1.25745114170563
2214	12	-1.379247494532784
2214	13	-1.324668156833745
2214	14	-1.5592261695332823
2214	15	-1.5415288827754239
2214	16	-1.6353725737691391
2214	17	-1.4353779012319947
2214	18	-1.614993841776915
2214	19	-1.5121745652751173
2214	20	-1.5993153562302496
2214	21	-1.5753421050607201
2214	22	-1.5669336036183523
2214	23	-1.5335379802023468
2214	24	-1.5852049895339206
2214	25	-1.634556300690619
2215	1	-1.0450475508162
2215	2	-1.2402433131605726
2215	3	-1.2802409566686528
2215	4	-1.023769574053361
2215	5	-1.1422470682331607
2215	6	-1.0554582147516385
2215	7	-1.0572071820682964
2215	8	-1.0528728396167182
2215	9	-1.4110338888938898
2215	10	-1.267477738680661
2215	11	-1.4842683152139813
2215	12	-1.476625981617154
2215	13	-1.6183119137127946
2215	14	-1.8443220685997659
2215	15	-1.908364043728234
2215	16	-1.5415334008858466
2215	17	-1.6336478623696067
2215	18	-1.6813452610695165
2215	19	-1.6897139542115625
2215	20	-1.633252857079519
2215	21	-1.6728169837856015
2215	22	-1.6089238404615358
2215	23	-1.6773492122488705
2215	24	-1.680989829804055
2215	25	-1.7379637239509833
2216	1	-1.8864802280763442
2216	2	-1.7869020046256026
2216	3	-1.8898247414284945
2216	4	-1.7303423440064094
2216	5	-1.8484925539680788
2216	6	-1.9011267774096368
2216	7	-2.0243169990900896
2216	8	-2.051398179691354
2216	9	-2.406339053213138
2216	10	-2.3484147496352747
2216	11	-2.414752394905804
2216	12	-2.4936071420842296
2216	13	-2.2587958429291177
2216	14	-2.6302556186178236
2216	15	-2.7259350681654553
2216	16	-2.560233743969995
2216	17	-2.771216328723284
2216	18	-2.6806982164083877
2216	19	-2.7327236147388305
2216	20	-2.8395021047020066
2216	21	-2.7943974279892814
2216	22	-2.8621741113174366
2216	23	-2.8763304930963542
2216	24	-2.947395421473729
2216	25	-2.9305422721633363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41917.0
3	3.0
4	729.0
5	5352.0
6	7073.0
7	5967.0
8	4477.0
9	3752.0
10	3786.0
11	3741.0
12	3233.0
13	2838.0
14	2736.0
15	2745.0
16	2860.0
17	3021.0
18	3825.0
19	5341.0
20	6912.0
21	8459.0
22	9805.0
23	11503.0
24	12765.0
25	14569.0
26	15647.0
27	17495.0
28	20178.0
29	24309.0
30	31529.0
31	40702.0
32	52368.0
33	68905.0
34	94174.0
35	130197.0
36	189942.0
37	335313.0
38	962686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69710926950081	19.162833091458552	13.439319313934789	24.700738325105856
2	16.237926947717575	22.500547480635923	36.65177116378807	24.60975440785843
3	17.816690486662505	26.893550189831572	29.4364006111061	25.85335871239982
4	12.375229919154009	17.652816033060002	37.231187545216656	32.74076650256933
5	14.40273336341635	37.02977240754013	32.978949804669945	15.588544424373577
6	30.48494430250098	38.96978348466475	16.142204559524856	14.403067653309407
7	26.825995565397832	32.64333068472248	22.03743421673572	18.493239533143978
8	26.27928731299219	32.9540097245456	20.162019967956795	20.604682994505417
9	26.879702219498746	14.91729300935539	19.959030074677592	38.24397469646827
10	16.033176353641814	27.051356024640032	30.93073350539262	25.98473411632553
11	34.74605861641385	23.511842631987356	23.112183151535383	18.62991560006342
12	24.12147051575918	25.64577996196782	29.047893584277553	21.184855937995454
13	28.839923374497843	21.535439294747803	25.79698891533998	23.827648415414373
14	23.09971105657321	21.47560162448142	26.840166019248635	28.584521299696735
15	23.906383599973406	28.15475523496857	24.437726458856496	23.501134706201526
16	23.517985307128985	27.448172550339656	24.972766441374738	24.061075701156618
17	23.402906904024924	25.93244068252124	26.283958556228676	24.380693857225157
18	24.599262150457147	24.103828960245856	28.521952999867494	22.774955889429506
19	25.505930946882536	24.783003082055963	27.852924233682053	21.858141737379448
20	24.705160853265536	24.585109652497806	27.59680464805517	23.112924846181492
21	25.073159300832348	24.80279302543349	27.632502722260604	22.491544951473553
22	23.803751948667234	24.799972475729128	27.82638694196517	23.569888633638474
23	22.696935446450865	24.314970250867226	28.858727768352427	24.12936653432948
24	23.07474142203699	25.557396729220077	28.245603267990266	23.122258580752668
25	23.020108577390683	25.01729650108522	28.463159382607905	23.499435538916195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4466.0
1	4466.0
2	4693.5
3	4921.0
4	4921.0
5	4921.0
6	10028.0
7	15135.0
8	15135.0
9	15135.0
10	15303.0
11	15471.0
12	15471.0
13	15471.0
14	14592.0
15	13713.0
16	13713.0
17	13713.0
18	22921.5
19	32130.0
20	32130.0
21	32130.0
22	46759.0
23	61388.0
24	61388.0
25	61388.0
26	82530.0
27	103672.0
28	103672.0
29	103672.0
30	117234.0
31	130796.0
32	130796.0
33	130796.0
34	145015.5
35	159235.0
36	159235.0
37	159235.0
38	169872.5
39	180510.0
40	180510.0
41	180510.0
42	197353.0
43	214196.0
44	214196.0
45	214196.0
46	225579.5
47	236963.0
48	236963.0
49	236963.0
50	240429.5
51	243896.0
52	243896.0
53	243896.0
54	228375.0
55	212854.0
56	212854.0
57	212854.0
58	194831.5
59	176809.0
60	176809.0
61	176809.0
62	155623.0
63	134437.0
64	134437.0
65	134437.0
66	111165.5
67	87894.0
68	87894.0
69	87894.0
70	68128.5
71	48363.0
72	48363.0
73	48363.0
74	39715.5
75	31068.0
76	31068.0
77	31068.0
78	26857.5
79	22647.0
80	22647.0
81	22647.0
82	17127.5
83	11608.0
84	11608.0
85	11608.0
86	8773.0
87	5938.0
88	5938.0
89	5938.0
90	4225.0
91	2512.0
92	2512.0
93	2512.0
94	1361.5
95	211.0
96	211.0
97	211.0
98	116.0
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4300617336183674
2	0.004323863916379261
3	0.0
4	0.004556329718335136
5	0.16100581443463852
6	0.04356409128653084
7	0.0019062195760381692
8	3.719452831293988E-4
9	3.719452831293988E-4
10	0.0020921922176028685
11	0.0010693426889970216
12	0.0020456990572116935
13	0.006230083492417431
14	0.012088221701705463
15	0.003579973350120464
16	6.973974058676228E-4
17	0.008415262030802648
18	4.184384435205737E-4
19	7.903837266499725E-4
20	0.004881781841073359
21	0.00316153490659989
22	0.0011623290097793712
23	0.0016272606136911198
24	0.0024176443403410926
25	0.006044110850852731
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2150854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.47674308236802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77060657942931	44.30374358493453
2	7.512377907830239	7.584007391824087
3	1.6733962894433265	2.534027837316562
4	0.7274817589565264	1.4688363937583104
5	0.4344761124022972	1.0965469550578402
6	0.2722091093919037	0.8244137567673199
7	0.21045071075772404	0.7436006520893564
8	0.1478214744440667	0.5969237270055988
9	0.1139943298064503	0.5178656248638227
>10	0.8579037806553971	8.815341490857701
>50	0.13241564301187467	4.691799310239994
>100	0.12487935652779779	13.083438302982966
>500	0.014936280684628428	5.139587598644945
>1k	0.0064940350802732295	4.993577119458993
>5k	2.7831578915456695E-4	1.252842963483307
>10k+	2.7831578915456695E-4	2.35344729071468
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	21855	1.0161080203491264	No Hit
TATCAACGCAGAGTACTTTTTTTTT	16572	0.7704846540025497	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	11830	0.5500140874275985	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	9724	0.45209949164378427	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8814	0.40979071568781517	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	8216	0.38198780577389263	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4735	0.2201451144522129	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2769	0.12873956112316318	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2667	0.12399725876326333	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2494	0.1159539420155901	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2453	0.11404772243955191	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2380	0.11065372173099616	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2358	0.10963087220239032	No Hit
GTACAAAGGGCAGGGACTTAATCAA	2281	0.10605089885226986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.29863207823497E-5
2	0.0	0.0	0.0	4.649316039117485E-5	9.29863207823497E-5
3	0.0	0.0	0.0	4.649316039117485E-5	9.29863207823497E-5
4	0.0	0.0	0.0	4.649316039117485E-5	9.29863207823497E-5
5	0.0	0.0	0.0	4.649316039117485E-5	9.29863207823497E-5
6	4.649316039117485E-5	0.0	0.0	4.649316039117485E-5	1.3947948117352455E-4
7	4.649316039117485E-5	0.0	0.0	4.649316039117485E-5	1.3947948117352455E-4
8	4.649316039117485E-5	0.0	0.0	4.649316039117485E-5	1.3947948117352455E-4
9	4.649316039117485E-5	0.0	0.0	9.29863207823497E-5	1.3947948117352455E-4
10	4.649316039117485E-5	0.0	0.0	1.3947948117352455E-4	1.3947948117352455E-4
11	4.649316039117485E-5	0.0	0.0	1.3947948117352455E-4	1.3947948117352455E-4
12	4.649316039117485E-5	0.0	0.0	1.3947948117352455E-4	1.3947948117352455E-4
13	4.649316039117485E-5	0.0	0.0	1.859726415646994E-4	1.3947948117352455E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2645	0.0	14.390225	1
ACGACTA	75	1.46144575E-5	12.681558	5
GTATCAA	7615	0.0	12.639841	1
ACTGTCG	100	1.9459894E-6	11.392439	8
TCGCAGT	110	5.0137896E-7	11.219827	16
GTATTAG	485	0.0	11.211236	1
CGAGCCG	485	0.0	11.157544	15
CGGTCCA	370	0.0	11.033218	10
TGTCGAG	95	1.3707975E-5	10.992704	10
AGGCCCG	520	0.0	10.954268	10
ATACCGT	375	0.0	10.89016	6
CGACCAT	315	0.0	10.849943	10
GCGAAAC	125	2.2609493E-7	10.633191	19
GCCGCCT	530	0.0	10.5684595	18
AGAACCG	280	0.0	10.530221	5
ATCGCCA	415	0.0	10.523136	16
TATTGGG	100	2.3668079E-5	10.463016	2
TAATACC	155	3.594323E-9	10.431603	4
TAGACAG	275	0.0	10.375819	5
CTGTCGA	110	6.0956027E-6	10.356763	9
>>END_MODULE