FastQCFastQC Report
Thu 2 Feb 2017
SRR4050261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692324
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN83440.225982335244686No Hit
GTATCAACGCAGAGTACTTTTTTTT82230.22270526638507346No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN63630.17233048887367414No Hit
TATCAACGCAGAGTACTTTTTTTTT50270.1361473153493572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.015.0082891
GTATCAA53350.013.3750441
CGTCTTA1103.8216967E-812.0904415
TAGATCG1201.0015356E-811.876165
CTCGACG907.4748186E-611.61018613
CGAATAC1408.352799E-910.8582034
CGAACGA2850.010.66625316
GTCTAAA3750.010.641621
CGGTCCA5900.010.46555210
ACGCTTA1002.401841E-510.449594517
TAATACT4550.010.4405794
CGTCGTA4250.010.28181510
GTAATAC2700.010.20469953
ACGAATA1502.4447218E-810.1343233
GCGTTAT2350.010.1079211
CGAGCCG5400.010.02738915
TCGACGC1054.1107116E-59.95185914
CGTTATT2400.09.897342
GTATTAG4900.09.8892591
CCGTCGT4900.09.8871029
Produced by FastQC (version 0.11.5)