##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050261_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3692324
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.88781943296417	31.0	30.0	34.0	26.0	34.0
2	29.994561419853728	31.0	31.0	34.0	26.0	34.0
3	30.118551893062474	31.0	31.0	34.0	26.0	34.0
4	33.49484335610851	37.0	35.0	37.0	28.0	37.0
5	33.57987516805134	37.0	35.0	37.0	28.0	37.0
6	33.642202580271935	37.0	35.0	37.0	29.0	37.0
7	33.5337697883501	37.0	35.0	37.0	28.0	37.0
8	33.47583825254772	37.0	35.0	37.0	28.0	37.0
9	34.94781660547666	39.0	35.0	39.0	28.0	39.0
10	34.848908979818674	39.0	35.0	39.0	27.0	39.0
11	34.87511090576017	39.0	35.0	39.0	27.0	39.0
12	34.772512108904856	39.0	35.0	39.0	27.0	39.0
13	34.76244094505249	39.0	35.0	39.0	27.0	39.0
14	35.61455847320008	40.0	36.0	41.0	26.0	41.0
15	35.74141245459499	40.0	36.0	41.0	27.0	41.0
16	35.76141774123831	40.0	36.0	41.0	27.0	41.0
17	35.751096328491215	40.0	36.0	41.0	27.0	41.0
18	35.57726163792777	40.0	36.0	41.0	26.0	41.0
19	35.70732687597297	40.0	36.0	41.0	27.0	41.0
20	35.67280200762447	40.0	36.0	41.0	27.0	41.0
21	35.63484000862329	40.0	36.0	41.0	27.0	41.0
22	35.55802226456833	40.0	36.0	41.0	26.0	41.0
23	35.44898199616285	40.0	36.0	41.0	26.0	41.0
24	35.38741047643706	40.0	36.0	41.0	25.0	41.0
25	35.099317665513645	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1247905329503851
1101	2	1.1125639627837884
1101	3	1.0635207699260647
1101	4	1.2182528035024163
1101	5	1.0819215518927905
1101	6	1.1198780084458875
1101	7	1.1764166066643114
1101	8	1.1750300349486693
1101	9	1.2694014297228549
1101	10	1.3555079990242618
1101	11	1.322377642204195
1101	12	1.2860412063020945
1101	13	1.3754822493806742
1101	14	1.7710534880138908
1101	15	1.6338798203343075
1101	16	1.6441450810354752
1101	17	1.647608808088492
1101	18	1.8033299246049808
1101	19	1.6161373978850122
1101	20	1.6188396020238471
1101	21	1.6758046967866704
1101	22	1.7009699035814876
1101	23	1.730204668917473
1101	24	1.689551482236297
1101	25	1.6975533949276098
1102	1	0.9677386667800647
1102	2	0.9201504395968279
1102	3	0.8050313082762202
1102	4	0.9439925395158468
1102	5	0.8715868830909699
1102	6	0.828121713021531
1102	7	0.8466658094663018
1102	8	0.8852915069317149
1102	9	1.0642062232679876
1102	10	1.0687790055153812
1102	11	1.0825724154533987
1102	12	1.1695377635619266
1102	13	1.1291585222584146
1102	14	1.4746767056464591
1102	15	1.3517110633258298
1102	16	1.308821491343977
1102	17	1.2709316299602946
1102	18	1.4139341767924165
1102	19	1.2868000133318347
1102	20	1.2616868039136264
1102	21	1.2328416995213445
1102	22	1.3272181085166466
1102	23	1.3621919774853808
1102	24	1.4239481113983032
1102	25	1.3722570225021968
1103	1	0.8494421512427515
1103	2	0.9050929941866315
1103	3	0.8119913457196155
1103	4	0.899644000802887
1103	5	0.8530394972667708
1103	6	0.8433367172819217
1103	7	0.8168647292970093
1103	8	0.8757184403219895
1103	9	1.0238712764417244
1103	10	0.9775557582892631
1103	11	1.0031457633163683
1103	12	1.0805961490970262
1103	13	1.0114361578252726
1103	14	1.430410035747812
1103	15	1.299106369569266
1103	16	1.2542046546242531
1103	17	1.1062232201794302
1103	18	1.2427152356995848
1103	19	1.2201333704297
1103	20	1.1478216607436025
1103	21	1.1644613961837251
1103	22	1.272021179559644
1103	23	1.2191086981272434
1103	24	1.2510447402449074
1103	25	1.3033922791704384
1104	1	0.7699053673821581
1104	2	0.7705077016865545
1104	3	0.7112048962414335
1104	4	0.8614610452992366
1104	5	0.7566478201123203
1104	6	0.7171480887973871
1104	7	0.7628797362599471
1104	8	0.7748556140111305
1104	9	0.8102919169478682
1104	10	0.9763700472351644
1104	11	0.8757315148255032
1104	12	0.9282795372780512
1104	13	0.9068585823027249
1104	14	1.256170654745766
1104	15	1.1893371517817357
1104	16	1.0788670106752463
1104	17	1.0419543596649277
1104	18	1.164432049255133
1104	19	1.159594669144056
1104	20	1.1090320293212912
1104	21	1.0080057360395358
1104	22	1.0730789799906475
1104	23	1.0570269978068936
1104	24	1.044079906203315
1104	25	1.1072250421835221
1105	1	0.6124365338187978
1105	2	0.6992215105203741
1105	3	0.6342896613912004
1105	4	0.795323915234114
1105	5	0.7281697230543145
1105	6	0.7322298564531025
1105	7	0.7238876728932198
1105	8	0.7600503933122056
1105	9	0.9230579660482263
1105	10	0.8977636986617696
1105	11	0.8052404534435667
1105	12	0.859102576272825
1105	13	0.9156866873416405
1105	14	1.235748446329545
1105	15	1.0807528663325385
1105	16	1.1864309806219069
1105	17	1.1637111988527096
1105	18	1.219193211388287
1105	19	1.147533555685989
1105	20	1.1122702901937984
1105	21	1.1222376426420269
1105	22	1.0962258016429942
1105	23	1.10993094178599
1105	24	1.0655883854255919
1105	25	1.1409707859337388
1106	1	0.4772190056326693
1106	2	0.38099378010099016
1106	3	0.3837009192425569
1106	4	0.5449105311450211
1106	5	0.42405683668616234
1106	6	0.3896080680696272
1106	7	0.34095564191522243
1106	8	0.38174250844166124
1106	9	0.5298123298871147
1106	10	0.6057442932769703
1106	11	0.6098537821188259
1106	12	0.6253284439167786
1106	13	0.6155805030190535
1106	14	0.912036379841112
1106	15	0.76586261989749
1106	16	0.7411108041889065
1106	17	0.6940023051375519
1106	18	0.8760780392422944
1106	19	0.8170604920276077
1106	20	0.7470597304069884
1106	21	0.8240714185092557
1106	22	0.7947757724818487
1106	23	0.7629584025859799
1106	24	0.8223232149549418
1106	25	0.7694702635163964
1107	1	0.20384578186326507
1107	2	0.23619443757605296
1107	3	0.21643222597097633
1107	4	0.28189760364452354
1107	5	0.19351175542392696
1107	6	0.1744607234452431
1107	7	0.1789987943519762
1107	8	0.16563727714677867
1107	9	0.11853871045324382
1107	10	0.17704082175048796
1107	11	0.12840649406460614
1107	12	0.11324943059983639
1107	13	0.05014770202467389
1107	14	0.50874426720587
1107	15	0.2941344886455468
1107	16	0.15864144690802107
1107	17	0.14262122035270863
1107	18	0.35288108472010293
1107	19	0.2417959493042261
1107	20	0.31652373173605497
1107	21	0.3084565981379157
1107	22	0.3272627803740633
1107	23	0.3715242009776105
1107	24	0.3622228261840945
1107	25	0.2317047672026007
1108	1	0.3764802617077905
1108	2	0.38326476122816544
1108	3	0.3000237053662005
1108	4	0.512437560197796
1108	5	0.26145255471048756
1108	6	0.3210021432896042
1108	7	0.29350349417679666
1108	8	0.2786358145481742
1108	9	0.33783992537390617
1108	10	0.37201875686444197
1108	11	0.3637812792121906
1108	12	0.3693812428843444
1108	13	0.35447951328933414
1108	14	0.7202681441139092
1108	15	0.5481263226955946
1108	16	0.5005011599496143
1108	17	0.4949317490544374
1108	18	0.6311815304119719
1108	19	0.5610119188941596
1108	20	0.49883986141043835
1108	21	0.3968578821122719
1108	22	0.538309375342152
1108	23	0.5727861541942261
1108	24	0.554902738308563
1108	25	0.45717998898934553
1109	1	0.1917946695397994
1109	2	0.25237546911677455
1109	3	0.27870316216667845
1109	4	0.3861570939314589
1109	5	0.25887717512097197
1109	6	0.34165683360176047
1109	7	0.27606022203313785
1109	8	0.257663595717041
1109	9	0.2858097243383497
1109	10	0.34009057720219005
1109	11	0.22233115787531688
1109	12	0.355824443743586
1109	13	0.32402965709123066
1109	14	0.6031782144670856
1109	15	0.45590416413721613
1109	16	0.46364803688854295
1109	17	0.41157008394862515
1109	18	0.5119142360580042
1109	19	0.47638148933132385
1109	20	0.3425072470967976
1109	21	0.4217095191202418
1109	22	0.410117787369515
1109	23	0.33113756526372384
1109	24	0.2999231046712012
1109	25	0.24816671867749562
1110	1	-0.12882801668568789
1110	2	-0.19840142910772585
1110	3	-0.2092744537492237
1110	4	-0.14890870650165766
1110	5	-0.21084202918795825
1110	6	-0.18359793510049371
1110	7	-0.11840596967128647
1110	8	-0.22327380405592123
1110	9	-0.11527927035614738
1110	10	-0.23537941617902192
1110	11	-0.24615368619101474
1110	12	-0.2025940140415301
1110	13	-0.21138273396923069
1110	14	0.014158523779833843
1110	15	-0.24910731646033213
1110	16	-0.23933603319289176
1110	17	-0.33348181409216693
1110	18	-0.09518915331480571
1110	19	-0.218365730088955
1110	20	-0.3321016971285502
1110	21	-0.2148438320842061
1110	22	-0.2760670523629827
1110	23	-0.21187132278964782
1110	24	-0.27894405547339574
1110	25	-0.4113457446115305
1111	1	-0.422251036914421
1111	2	-0.4557237483402652
1111	3	-0.5130245664110582
1111	4	-0.373539092235319
1111	5	-0.4009822531935967
1111	6	-0.4485097045982087
1111	7	-0.4414751235235741
1111	8	-0.42925770070919356
1111	9	-0.5279390792149172
1111	10	-0.5055553333917189
1111	11	-0.4543935384691409
1111	12	-0.48223274455776277
1111	13	-0.5360877860973403
1111	14	-0.3434997780754827
1111	15	-0.4636201849991295
1111	16	-0.5435817233191713
1111	17	-0.5156029922447445
1111	18	-0.4331934166431708
1111	19	-0.5710731298904506
1111	20	-0.5853988526878169
1111	21	-0.5497519005666618
1111	22	-0.6774625623218569
1111	23	-0.5653727395021022
1111	24	-0.6557079467447409
1111	25	-0.7863292289520984
1112	1	-0.559091016554305
1112	2	-0.4964179379145115
1112	3	-0.5524831597896842
1112	4	-0.40754097786663124
1112	5	-0.5574607718623952
1112	6	-0.624727007393524
1112	7	-0.6277996503195951
1112	8	-0.5556797649465111
1112	9	-0.6879036547203725
1112	10	-0.6312358068424473
1112	11	-0.6246881217479654
1112	12	-0.5506118585968096
1112	13	-0.6772257647974484
1112	14	-0.5436432828467659
1112	15	-0.5795221633818599
1112	16	-0.7509139694690461
1112	17	-0.7398677677945287
1112	18	-0.6861836440508355
1112	19	-0.7652989312001637
1112	20	-0.7225555531289132
1112	21	-0.7240472436511425
1112	22	-0.7275943325646566
1112	23	-0.7911551250020352
1112	24	-0.7781445780108953
1112	25	-0.7066589752826005
1113	1	-1.0031994843530576
1113	2	-1.0717784071311307
1113	3	-0.9887523686557245
1113	4	-0.9174060712698378
1113	5	-0.9944983269067507
1113	6	-1.1024847819747592
1113	7	-1.1929818238080756
1113	8	-1.203818854683469
1113	9	-1.178745609067093
1113	10	-1.2686450600808143
1113	11	-1.3077887640708568
1113	12	-1.2645641087412116
1113	13	-1.3374104188773543
1113	14	-1.2533235766014101
1113	15	-1.3455755870296642
1113	16	-1.3513026474486196
1113	17	-1.453695268987424
1113	18	-1.4035413785595807
1113	19	-1.45535613508698
1113	20	-1.6088198859286464
1113	21	-1.4102633211673705
1113	22	-1.437525502530157
1113	23	-1.8880131223202383
1113	24	-1.787600108443364
1113	25	-1.758654043261835
1114	1	-2.556135319259173
1114	2	-2.8342324321615138
1114	3	-2.365213016600677
1114	4	-5.359884508055501
1114	5	-3.0007503193769196
1114	6	-2.495118026239858
1114	7	-2.37766798721886
1114	8	-2.5741131830831634
1114	9	-3.1443927535607337
1114	10	-2.92539974286759
1114	11	-2.6343663634599537
1114	12	-2.701363541623799
1114	13	-2.734865171576878
1114	14	-2.5577171083099444
1114	15	-3.3395635888932986
1114	16	-3.263314781684734
1114	17	-3.008284845154513
1114	18	-2.956100568661668
1114	19	-2.973655709025799
1114	20	-3.1727025081791
1114	21	-3.2314170677679286
1114	22	-3.275262994079597
1114	23	-3.401655350366049
1114	24	-3.4049743860599406
1114	25	-3.2983966308418076
1115	1	-2.843912657028053
1115	2	-2.247209816451722
1115	3	-1.8920774021202398
1115	4	-1.7986726274307685
1115	5	-1.841384729973786
1115	6	-1.8102318833482478
1115	7	-1.8921708674081756
1115	8	-1.8594332527113693
1115	9	-2.199432291884598
1115	10	-2.153107471655929
1115	11	-2.0952791234723733
1115	12	-2.404554385764854
1115	13	-2.6773986424236753
1115	14	-2.84149760068631
1115	15	-2.6525801414513452
1115	16	-2.5389496680679216
1115	17	-2.683372529209329
1115	18	-2.4952558124516955
1115	19	-2.4719134660249082
1115	20	-2.589818272277512
1115	21	-2.4984233529532887
1115	22	-2.562396049886928
1115	23	-2.5647416368586136
1115	24	-2.8168075282777565
1115	25	-2.9573835616163393
1116	1	-1.4330343180521616
1116	2	-1.3704820072251138
1116	3	-1.5692888146012294
1116	4	-1.4107156438707733
1116	5	-1.5553865659538886
1116	6	-1.6374013408152308
1116	7	-1.7208158549270074
1116	8	-1.6685580872557644
1116	9	-1.985232716006763
1116	10	-2.321030958440325
1116	11	-2.0628916904334247
1116	12	-2.030932390579821
1116	13	-1.9424025577767452
1116	14	-2.8067496602247743
1116	15	-2.6444107023620944
1116	16	-2.653745063415485
1116	17	-2.327678167003981
1116	18	-2.341823883341327
1116	19	-2.1415648853011717
1116	20	-2.2809746618334046
1116	21	-2.430451703067881
1116	22	-2.3480232078695167
1116	23	-2.7264538317378637
1116	24	-2.7604235753534354
1116	25	-2.7158864668665856
1201	1	1.099363113159228
1201	2	1.1021354609172072
1201	3	0.9980795536732927
1201	4	1.1718128316363803
1201	5	1.1526460013869197
1201	6	1.150631944495224
1201	7	1.1632883266231886
1201	8	1.141982775304669
1201	9	1.3366814978936077
1201	10	1.3615013442604251
1201	11	1.3135323519374609
1201	12	1.372717476388118
1201	13	1.3534640434617273
1201	14	1.7368267661613785
1201	15	1.6033301260206088
1201	16	1.6245419321959957
1201	17	1.502618817286134
1201	18	1.6961395334347174
1201	19	1.5745667654119302
1201	20	1.6139432107453757
1201	21	1.5328618574207695
1201	22	1.6177224599846696
1201	23	1.7247726503057734
1201	24	1.7552551017785802
1201	25	1.6759977762509877
1202	1	0.9771847270918563
1202	2	1.0028161529759103
1202	3	0.9057242333985336
1202	4	1.0843041351747331
1202	5	0.9954759116623038
1202	6	0.9658230706747588
1202	7	0.938064300871865
1202	8	0.950543849779045
1202	9	1.1259395304845157
1202	10	1.0824538966923356
1202	11	1.0899968279123655
1202	12	1.1463384135482215
1202	13	1.232994001751372
1202	14	1.5649708840870318
1202	15	1.4760227465217213
1202	16	1.4249004877177995
1202	17	1.3974017976982722
1202	18	1.4832821211097453
1202	19	1.4160112436078975
1202	20	1.4273177563246335
1202	21	1.402082711617446
1202	22	1.3545280903923924
1202	23	1.4532264929169685
1202	24	1.428470816488911
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1203	1	0.8889536749927451
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2211	12	-0.16951379481661633
2211	13	-0.1758145158022657
2211	14	0.12123840387988594
2211	15	-0.01814072637976949
2211	16	-0.13820416266796087
2211	17	-0.29104524773328677
2211	18	-0.07682731055439973
2211	19	-0.12492445419517395
2211	20	-0.1349828934999664
2211	21	-0.18143932911161187
2211	22	-0.18139437027883787
2211	23	-0.13223767188627278
2211	24	-0.03178618965477398
2211	25	-0.06888407023005527
2212	1	-0.3578381513877886
2212	2	-0.41242074029747045
2212	3	-0.3760589769535194
2212	4	-0.3394272800679019
2212	5	-0.39730931427740046
2212	6	-0.37400441293746667
2212	7	-0.39447014699664606
2212	8	-0.3969076883625675
2212	9	-0.4078781678334451
2212	10	-0.5221334935111557
2212	11	-0.5239374648108281
2212	12	-0.5131887166902089
2212	13	-0.44057745457242703
2212	14	-0.5345066249138242
2212	15	-0.580704246141508
2212	16	-0.61212586306182
2212	17	-0.7124241872859756
2212	18	-0.6229557695263921
2212	19	-0.6964468144267073
2212	20	-0.7104929529895756
2212	21	-0.7789081015825019
2212	22	-0.7932265801879268
2212	23	-0.8620270326388493
2212	24	-0.6669673829067051
2212	25	-0.7222332883516671
2213	1	-0.8672790778144375
2213	2	-0.7052396517247743
2213	3	-0.7160855465490208
2213	4	-0.6472339912788101
2213	5	-0.7110079172629753
2213	6	-0.8258866243254701
2213	7	-0.8912568257049145
2213	8	-0.7941689098709972
2213	9	-0.7951194311283913
2213	10	-0.9438081597533738
2213	11	-0.9966966695426223
2213	12	-0.879711878692973
2213	13	-0.9885599037714812
2213	14	-0.8417282018340941
2213	15	-0.9903565007619761
2213	16	-0.9539841168268239
2213	17	-1.0292314611880826
2213	18	-0.9417615882082941
2213	19	-0.9342243440696194
2213	20	-1.018541671345254
2213	21	-1.008713264774805
2213	22	-1.102052888656786
2213	23	-1.0705360209965349
2213	24	-1.0738013650975802
2213	25	-1.0611749487683113
2214	1	-1.3625374344547048
2214	2	-1.227744473728869
2214	3	-1.2124587043547983
2214	4	-1.2266134028682956
2214	5	-1.1920520489875628
2214	6	-1.311317880877887
2214	7	-1.2387251913466386
2214	8	-1.2827565334826332
2214	9	-1.3382923955115444
2214	10	-1.4564288274685069
2214	11	-1.4547427016497565
2214	12	-1.4109163742471864
2214	13	-1.631000721379067
2214	14	-1.4074803723060043
2214	15	-1.6285374073683485
2214	16	-1.550779341306182
2214	17	-1.5730683857357377
2214	18	-1.4902292640630463
2214	19	-1.6437208418249654
2214	20	-1.6605292236883429
2214	21	-1.6551807600339288
2214	22	-1.7256638276825313
2214	23	-1.734112335779038
2214	24	-1.8163246844721144
2214	25	-1.7038681058021652
2215	1	-1.618947103673758
2215	2	-1.7882073575282895
2215	3	-1.6562004062055777
2215	4	-1.8662201469103188
2215	5	-1.7410858706343397
2215	6	-1.5378377027199335
2215	7	-1.6883409554130182
2215	8	-1.763941480090601
2215	9	-2.205369662935908
2215	10	-1.7761420823907343
2215	11	-1.9681551951793068
2215	12	-2.0629505595931263
2215	13	-2.0257226411785254
2215	14	-2.7874391704881205
2215	15	-2.426526894538277
2215	16	-2.3217088504215866
2215	17	-2.205775155600165
2215	18	-2.0421622502945027
2215	19	-2.314946797829961
2215	20	-2.5927567943655916
2215	21	-2.3804753836765613
2215	22	-2.7078710516364097
2215	23	-2.3601423158979244
2215	24	-2.3788382756794775
2215	25	-2.4080484844341328
2216	1	-1.8738682950041863
2216	2	-1.77088282398298
2216	3	-1.7203856488561051
2216	4	-1.6833965172208458
2216	5	-2.269769928429792
2216	6	-1.9731717329067173
2216	7	-2.098771933804745
2216	8	-2.538936477934268
2216	9	-2.4910994674304803
2216	10	-2.451958728480463
2216	11	-2.4188294719819368
2216	12	-2.5722540848909716
2216	13	-2.542555720811123
2216	14	-2.4901805371015087
2216	15	-2.504723885060706
2216	16	-2.570744801101192
2216	17	-2.6784118751380674
2216	18	-2.950191604138432
2216	19	-2.783724407218827
2216	20	-2.8352325282723783
2216	21	-2.8729551343351005
2216	22	-3.150505976061943
2216	23	-2.9478741503837043
2216	24	-3.0717157945729525
2216	25	-3.0176917633565665
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	143865.0
3	10.0
4	1004.0
5	6297.0
6	8157.0
7	7678.0
8	7149.0
9	6291.0
10	5796.0
11	5656.0
12	5467.0
13	5241.0
14	5210.0
15	5508.0
16	5518.0
17	6022.0
18	6460.0
19	7941.0
20	10221.0
21	13297.0
22	16404.0
23	19418.0
24	22488.0
25	25998.0
26	29131.0
27	32618.0
28	36824.0
29	44521.0
30	54624.0
31	70536.0
32	90356.0
33	118158.0
34	159756.0
35	221835.0
36	323772.0
37	558776.0
38	1604321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.076675151562206	17.870807896517753	12.39545784367831	25.657059108241732
2	16.3647952785272	20.638013875651307	38.22854257678827	24.768648269033218
3	18.38697286358567	24.94585066861645	29.083363935032253	27.583812532765627
4	12.529465876293214	16.77492405533341	37.96349966546722	32.73211040290616
5	15.192564754080925	35.77537193408999	33.75717376448728	15.2748895473418
6	31.91977307109201	37.05231872046219	17.04020428183497	13.987703926610829
7	28.322298502757388	31.094469021842585	22.172564424084385	18.410668051315643
8	26.22416372468867	33.23718841985103	20.508945376563183	20.029702478897114
9	26.95681219843729	15.292301945134845	19.392614022912973	38.35827183351489
10	15.900342239859121	26.697708484499948	31.944795519114688	25.45715375652624
11	35.196094552536714	22.566710379885045	23.296909287300018	18.94028578027822
12	24.333641028018395	24.31307956930016	29.19334452305733	22.159934879624114
13	28.868651414117565	20.793791724225944	25.310738283949156	25.02681857770734
14	23.09136176525843	20.590391757648334	26.27590424750177	30.042342229591462
15	24.454782193741906	27.418228918126665	23.17221351148681	24.954775376644626
16	24.699735528373296	26.87122266176593	24.15470253896034	24.27433927090043
17	24.133736098585675	26.00055686104899	25.402190301945772	24.463516738419568
18	24.89552681617001	25.101569067920593	26.420937557690333	23.58196655821907
19	25.87723256953521	25.34348010208214	25.451463129113616	23.327824199269042
20	24.9878100661038	25.41437594083382	25.459590505486833	24.138223487575537
21	25.40223334196856	25.2315558403665	25.302128355807646	24.0640824618573
22	24.68097836120435	25.114239675206722	25.68229521599118	24.522486747597743
23	24.010179638105793	24.73056465793711	25.8651383392311	25.394117364726004
24	24.151018728436444	25.732189331393755	25.705108328103943	24.411683612065854
25	24.356539100662673	25.23964640804433	25.800716498721943	24.60309799257106
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13961.0
1	13961.0
2	12991.0
3	12021.0
4	12021.0
5	12021.0
6	9271.0
7	6521.0
8	6521.0
9	6521.0
10	8502.0
11	10483.0
12	10483.0
13	10483.0
14	14416.0
15	18349.0
16	18349.0
17	18349.0
18	31029.0
19	43709.0
20	43709.0
21	43709.0
22	62765.5
23	81822.0
24	81822.0
25	81822.0
26	111248.0
27	140674.0
28	140674.0
29	140674.0
30	169318.5
31	197963.0
32	197963.0
33	197963.0
34	233011.0
35	268059.0
36	268059.0
37	268059.0
38	297825.5
39	327592.0
40	327592.0
41	327592.0
42	358854.5
43	390117.0
44	390117.0
45	390117.0
46	412182.0
47	434247.0
48	434247.0
49	434247.0
50	438655.0
51	443063.0
52	443063.0
53	443063.0
54	414015.5
55	384968.0
56	384968.0
57	384968.0
58	352005.5
59	319043.0
60	319043.0
61	319043.0
62	277731.5
63	236420.0
64	236420.0
65	236420.0
66	195351.5
67	154283.0
68	154283.0
69	154283.0
70	121144.5
71	88006.0
72	88006.0
73	88006.0
74	70700.0
75	53394.0
76	53394.0
77	53394.0
78	44328.5
79	35263.0
80	35263.0
81	35263.0
82	26873.5
83	18484.0
84	18484.0
85	18484.0
86	13976.5
87	9469.0
88	9469.0
89	9469.0
90	6720.5
91	3972.0
92	3972.0
93	3972.0
94	2178.0
95	384.0
96	384.0
97	384.0
98	220.5
99	57.0
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2308302304998153
2	0.6585554247135409
3	0.656145018692834
4	0.6641345667389968
5	0.6809261592427966
6	0.694007351467531
7	0.6892677890672649
8	0.684934474872736
9	0.6762407632699623
10	0.6774324246734578
11	0.6836073974006615
12	0.6843657273847041
13	0.6795178321295747
14	0.6723949469223177
15	0.6790845007101218
16	0.6876427962443166
17	0.6863157187722421
18	0.6801949124724699
19	0.6792740832061326
20	0.6869386326877056
21	0.6817928220817024
22	0.6839323959652511
23	0.6878052955266114
24	0.6918948607976981
25	0.6783261707260793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3692324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.26547979883918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.55035960102879	49.56347869314981
2	7.863155751744035	9.005747737132484
3	2.1251182567365596	3.6508774980387537
4	0.9708776623704892	2.2239110064648586
5	0.545526435267825	1.5619916529281193
6	0.3488214832997499	1.1985257763181767
7	0.22883065164666588	0.9172867941459146
8	0.17477028177774878	0.8006643232464883
9	0.14215592082985729	0.7326564311310817
>10	0.8756603530930371	9.573455817855873
>50	0.08918346517615716	3.5591936587348787
>100	0.07044872813324783	8.120101474314032
>500	0.00996512080168924	3.820030178117458
>1k	0.004934651156066674	4.505065998379798
>5k	1.9163693809967665E-4	0.7670129600422884
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	8344	0.225982335244686	No Hit
GTATCAACGCAGAGTACTTTTTTTT	8223	0.22270526638507346	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	6363	0.17233048887367414	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5027	0.1361473153493572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7083213715806087E-5
2	0.0	0.0	0.0	0.0	2.7083213715806087E-5
3	0.0	0.0	0.0	0.0	2.7083213715806087E-5
4	0.0	0.0	0.0	2.7083213715806087E-5	2.7083213715806087E-5
5	2.7083213715806087E-5	0.0	0.0	5.4166427431612175E-5	2.7083213715806087E-5
6	2.7083213715806087E-5	0.0	0.0	5.4166427431612175E-5	2.7083213715806087E-5
7	2.7083213715806087E-5	0.0	0.0	5.4166427431612175E-5	2.7083213715806087E-5
8	2.7083213715806087E-5	0.0	0.0	5.4166427431612175E-5	5.4166427431612175E-5
9	2.7083213715806087E-5	0.0	0.0	1.3541606857903044E-4	5.4166427431612175E-5
10	2.7083213715806087E-5	0.0	0.0	1.6249928229483652E-4	5.4166427431612175E-5
11	2.7083213715806087E-5	0.0	0.0	1.8958249601064262E-4	5.4166427431612175E-5
12	2.7083213715806087E-5	0.0	0.0	1.8958249601064262E-4	1.6249928229483652E-4
13	2.7083213715806087E-5	0.0	0.0	1.8958249601064262E-4	1.6249928229483652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1665	0.0	15.008289	1
GTATCAA	5335	0.0	13.375044	1
CGTCTTA	110	3.8216967E-8	12.09044	15
TAGATCG	120	1.0015356E-8	11.87616	5
CTCGACG	90	7.4748186E-6	11.610186	13
CGAATAC	140	8.352799E-9	10.858203	4
CGAACGA	285	0.0	10.666253	16
GTCTAAA	375	0.0	10.64162	1
CGGTCCA	590	0.0	10.465552	10
ACGCTTA	100	2.401841E-5	10.4495945	17
TAATACT	455	0.0	10.440579	4
CGTCGTA	425	0.0	10.281815	10
GTAATAC	270	0.0	10.2046995	3
ACGAATA	150	2.4447218E-8	10.134323	3
GCGTTAT	235	0.0	10.107921	1
CGAGCCG	540	0.0	10.027389	15
TCGACGC	105	4.1107116E-5	9.951859	14
CGTTATT	240	0.0	9.89734	2
GTATTAG	490	0.0	9.889259	1
CCGTCGT	490	0.0	9.887102	9
>>END_MODULE