FastQCFastQC Report
Thu 2 Feb 2017
SRR4050261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692324
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT134300.36372756020327573No Hit
TATCAACGCAGAGTACTTTTTTTTT91480.24775723907219407No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48720.13194941722340725No Hit
GGTATCAACGCAGAGTACTTTTTTT48610.13165150187253338No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44570.12070988353134775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.016.545621
AACCCGC456.7806034E-414.77341656
GTATCAA56850.013.361141
TAGATCG1850.012.83978755
GCGTTAT2100.012.2535011
CGACCAT3900.012.17754210
TATAACG1053.4668901E-610.8579092
CCGTCGT3500.010.8554099
CGTTATT2200.010.7962172
ATTTCGT1158.874631E-710.734652517
CGAGCCG5700.010.66424115
CGTCGTA3500.010.58402310
TATCACG909.515479E-510.55632
AACCGCG3700.010.5243477
TACCGTC4000.010.447347
ACGCATC2750.010.36198110
ACCGTCG4050.010.3183618
AGGCCCG6400.010.24055210
ATACCGT3950.010.0982856
ACGGTAT4900.010.0800229
Produced by FastQC (version 0.11.5)