FastQCFastQC Report
Thu 2 Feb 2017
SRR4050260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643994
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN127480.3498359217935046No Hit
GTATCAACGCAGAGTACTTTTTTTT78220.214654579562974No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN57030.156504099622557No Hit
TATCAACGCAGAGTACTTTTTTTTT47280.12974774382175164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.015.2823781
GTATCAA49950.013.6854271
AACCGCG3600.011.34656057
GTATTAC2550.010.8124161
CTCGACG1158.8319575E-710.73865313
GTATTAG6050.010.6860821
GCGTTAT2800.010.5261271
GCTCGTA2900.010.4818589
CGTTATT2650.010.39677052
CGAGCCG6200.010.11245415
TACCGTG856.6009857E-410.0582377
ATACCGT4350.010.0455316
AGAACCG4600.09.9128775
GTATAAA5100.09.8803111
ACCGTCC1856.511982E-109.7560548
GCCGCCT6350.09.72397818
CTAGAAC2550.09.6861113
TAGAACC5600.09.4998414
ATCCGTG1304.245665E-69.4994467
CTCGTAG3400.09.49931510
Produced by FastQC (version 0.11.5)