##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050260_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3643994
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.966016958315517	31.0	31.0	34.0	26.0	34.0
2	30.008590848393276	31.0	31.0	34.0	26.0	34.0
3	30.16013829880071	31.0	31.0	34.0	26.0	34.0
4	33.59576278116813	37.0	35.0	37.0	28.0	37.0
5	33.63978124003497	37.0	35.0	37.0	28.0	37.0
6	33.681525820294986	37.0	35.0	37.0	29.0	37.0
7	33.586044323892956	37.0	35.0	37.0	28.0	37.0
8	33.55795755975449	37.0	35.0	37.0	28.0	37.0
9	35.04754563262179	39.0	35.0	39.0	28.0	39.0
10	34.937188425667	39.0	35.0	39.0	28.0	39.0
11	34.962758994663545	39.0	35.0	39.0	28.0	39.0
12	34.89151930546538	39.0	35.0	39.0	27.0	39.0
13	34.883661444008965	39.0	35.0	39.0	27.0	39.0
14	35.9620114632461	40.0	36.0	41.0	27.0	41.0
15	35.94357866670472	40.0	36.0	41.0	27.0	41.0
16	35.94308744745463	40.0	36.0	41.0	27.0	41.0
17	35.90850863091432	40.0	36.0	41.0	27.0	41.0
18	35.83983535647973	40.0	36.0	41.0	27.0	41.0
19	35.878728944120105	40.0	37.0	41.0	27.0	41.0
20	35.84737708130145	40.0	37.0	41.0	27.0	41.0
21	35.76683578513027	40.0	36.0	41.0	27.0	41.0
22	35.673055444108854	40.0	36.0	41.0	27.0	41.0
23	35.59953007606489	40.0	36.0	41.0	27.0	41.0
24	35.540310165164925	40.0	36.0	41.0	26.0	41.0
25	35.203664440720814	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.3751313766203985
1101	2	1.4579734076514121
1101	3	1.4476470138827864
1101	4	1.505492419881925
1101	5	1.5240885832036497
1101	6	1.439105668377735
1101	7	1.4145043702007456
1101	8	1.4525665398872647
1101	9	1.5613555417460816
1101	10	1.638589740073492
1101	11	1.5950543447299168
1101	12	1.6984606401058073
1101	13	1.7194128427133393
1101	14	1.943294454719826
1101	15	1.9389553882232633
1101	16	1.9217749504869062
1101	17	1.879590851163698
1101	18	1.979827623927413
1101	19	2.0115085241430606
1101	20	2.0088929923513206
1101	21	2.0339753330898205
1101	22	2.0528858816134274
1101	23	2.1448019755062546
1101	24	2.106724442135736
1101	25	2.029113258751714
1102	1	1.0359239920855572
1102	2	1.0845884115734918
1102	3	1.1204675470722663
1102	4	1.1778641841217592
1102	5	1.1622724998214267
1102	6	1.0675792642807806
1102	7	1.1141996849986668
1102	8	1.0578380068258681
1102	9	1.2425603422396492
1102	10	1.3451354413665086
1102	11	1.2287321119138852
1102	12	1.2257054515948553
1102	13	1.2667767111743018
1102	14	1.5190144928855247
1102	15	1.4955815520960627
1102	16	1.4824956334380914
1102	17	1.5123448340920191
1102	18	1.5974487889191877
1102	19	1.5566362905308964
1102	20	1.6164183213975107
1102	21	1.6178006397916178
1102	22	1.698676998411642
1102	23	1.6430181418969596
1102	24	1.6486462598393885
1102	25	1.6891736446186485
1103	1	0.9836704501765325
1103	2	1.0957058768391832
1103	3	1.0641971921517452
1103	4	1.1127655715375937
1103	5	1.0942234173060967
1103	6	1.1524264806040136
1103	7	1.0863755531423962
1103	8	1.1124734871388142
1103	9	1.2579581797981163
1103	10	1.308424234023697
1103	11	1.2855540418721745
1103	12	1.2000192640414085
1103	13	1.2668288442273123
1103	14	1.450168781450735
1103	15	1.4809587809980727
1103	16	1.4710154378841196
1103	17	1.5365386467668287
1103	18	1.5165405324946732
1103	19	1.474582900415058
1103	20	1.472804233163444
1103	21	1.5618857597384022
1103	22	1.5924260456281303
1103	23	1.552308708463812
1103	24	1.537659840520078
1103	25	1.5807754133109384
1104	1	0.8820511051419793
1104	2	1.0515661982755056
1104	3	1.0699509658562114
1104	4	1.1443388564055468
1104	5	1.1264125249144001
1104	6	1.124705275859334
1104	7	1.0893995390359592
1104	8	1.114358897417226
1104	9	1.2189137232425509
1104	10	1.3127314618875232
1104	11	1.2578477471396852
1104	12	1.223475224377637
1104	13	1.221835175096956
1104	14	1.467730931678524
1104	15	1.411360648987177
1104	16	1.4962122990911269
1104	17	1.5088238376326473
1104	18	1.5242261729365936
1104	19	1.58218225897064
1104	20	1.427815978721732
1104	21	1.5940351203931584
1104	22	1.6343686946586047
1104	23	1.6553772674464469
1104	24	1.6694171463279588
1104	25	1.6317427395886952
1105	1	0.6973706762235139
1105	2	0.7662226528550171
1105	3	0.7790036306327188
1105	4	0.8727424841090681
1105	5	0.7723382151162497
1105	6	0.7966413008782283
1105	7	0.7550527088654704
1105	8	0.7488854863679677
1105	9	0.890124471620318
1105	10	0.9160810576815805
1105	11	0.7710338586171375
1105	12	0.8640161031086535
1105	13	0.894548164345494
1105	14	0.9887111414086078
1105	15	1.0165902776632407
1105	16	1.0982556767557625
1105	17	0.9261615052215859
1105	18	0.9979207912608814
1105	19	1.0956172810702753
1105	20	1.0921884366338546
1105	21	1.1118925445688106
1105	22	1.1518579291616646
1105	23	1.156030976657327
1105	24	1.0863947262175415
1105	25	0.9876829788339663
1106	1	0.7680243056795675
1106	2	0.8547730042038353
1106	3	0.7849011272041544
1106	4	0.8582691500545536
1106	5	0.9070134221520973
1106	6	0.7957102403326388
1106	7	0.8184502476160631
1106	8	0.8209628572582801
1106	9	0.8842607892623917
1106	10	0.9778564357450463
1106	11	1.0289291230251436
1106	12	1.0914446902543986
1106	13	0.9342084087690878
1106	14	1.092923002981017
1106	15	1.0956097703876537
1106	16	1.1351371300778936
1106	17	1.1565401521057765
1106	18	1.1566225920059878
1106	19	1.2002220113455593
1106	20	1.1689521597815045
1106	21	1.2323870487312263
1106	22	1.1885206179913652
1106	23	1.2611595082400129
1106	24	1.2758868849353675
1106	25	1.1972554636767612
1107	1	0.6088435290706897
1107	2	0.6800450710944226
1107	3	0.6336762937876479
1107	4	0.7276387091341547
1107	5	0.7172807251333921
1107	6	0.6479324859524525
1107	7	0.5961018260371063
1107	8	0.5451263074939519
1107	9	0.6661088058017199
1107	10	0.6047657391034704
1107	11	0.6037073700496336
1107	12	0.6589117454756135
1107	13	0.6996133567334013
1107	14	0.8069141217538132
1107	15	0.7115803120435658
1107	16	0.7586161870385268
1107	17	0.7729179822805392
1107	18	0.7875462070597621
1107	19	0.7838923552281898
1107	20	0.845009735037884
1107	21	0.8617233187125706
1107	22	0.8718246100594413
1107	23	0.8292175458146929
1107	24	0.697405525583271
1107	25	0.6809918978138114
1108	1	0.6224463109399032
1108	2	0.7114244436716106
1108	3	0.650212841757142
1108	4	0.7605948061861696
1108	5	0.674266294267845
1108	6	0.6514552467321124
1108	7	0.6649195531107424
1108	8	0.6924038386065092
1108	9	0.7621335843080175
1108	10	0.8568194596763448
1108	11	0.811113535140521
1108	12	0.760552206077648
1108	13	0.8574128202444129
1108	14	0.9250300356819068
1108	15	0.8823083674542929
1108	16	0.7437155407147173
1108	17	0.8590402381500013
1108	18	0.8337265173868289
1108	19	0.8716897217515225
1108	20	0.9092783576059205
1108	21	0.8610886022302324
1108	22	0.859080888963895
1108	23	0.7093158037214309
1108	24	0.7920504842123108
1108	25	0.8782985034307984
1109	1	0.35339394424904924
1109	2	0.4917588653569105
1109	3	0.4581238949718305
1109	4	0.4596341176065337
1109	5	0.5105005977324311
1109	6	0.2917945783523024
1109	7	0.3515816946615189
1109	8	0.31716009609056073
1109	9	0.4517102218065503
1109	10	0.47084650610221246
1109	11	0.40504407581820345
1109	12	0.5112758848863734
1109	13	0.47170790644228333
1109	14	0.4943547030944657
1109	15	0.49602765392873494
1109	16	0.5141462706585855
1109	17	0.48958040147454795
1109	18	0.49453590224603516
1109	19	0.5843243202948045
1109	20	0.5653735509053917
1109	21	0.589712557416469
1109	22	0.627729322702919
1109	23	0.5584103963603511
1109	24	0.5328054140072922
1109	25	0.634066613276687
1110	1	0.13138167710740234
1110	2	0.3874077498791131
1110	3	0.29658827298419155
1110	4	0.23290413082193595
1110	5	0.2973514554735601
1110	6	0.2992050325301534
1110	7	0.18411242151560003
1110	8	0.22146665550801714
1110	9	0.21810335341857012
1110	10	0.26945778603109005
1110	11	0.28326928129924767
1110	12	0.18005569650171793
1110	13	0.18386091052212095
1110	14	0.21383020852893964
1110	15	0.22491739415205814
1110	16	0.18110610354467127
1110	17	0.23713462391688012
1110	18	0.2593974599479054
1110	19	0.30341503952118387
1110	20	0.20478714512324814
1110	21	0.2824733835247457
1110	22	0.22456307430013567
1110	23	0.2771626125556068
1110	24	0.24335793695502161
1110	25	0.36097774029067864
1111	1	-0.1640436889594561
1111	2	-0.09801587054003846
1111	3	-0.22693329986734412
1111	4	-0.1649817689651698
1111	5	-0.06646732599797645
1111	6	-0.22245082921410386
1111	7	-0.2678627029630718
1111	8	-0.21010634617677226
1111	9	-0.19933789314809758
1111	10	-0.2183933714124251
1111	11	-0.14962779126918235
1111	12	-0.24608707712925337
1111	13	-0.13850079791422587
1111	14	-0.19666353712750606
1111	15	-0.17641085765877307
1111	16	-0.19493428576048188
1111	17	-0.2131811259538381
1111	18	-0.18371404358291699
1111	19	-0.2886845787009875
1111	20	-0.27786415003647136
1111	21	-0.14300152016944878
1111	22	-0.1314508673223429
1111	23	-0.2704443914597192
1111	24	-0.25102652756014976
1111	25	-0.370342107911803
1112	1	-0.19978022001382456
1112	2	-0.06674024590104466
1112	3	-0.17114291434556606
1112	4	-0.12460419107999599
1112	5	-0.13704247197702557
1112	6	-0.15145048457137733
1112	7	-0.2733128309944419
1112	8	-0.22517763870423124
1112	9	-0.3530958327370328
1112	10	-0.3014132396597091
1112	11	-0.19671364158121918
1112	12	-0.3660860615725525
1112	13	-0.23487836444535048
1112	14	-0.43167605299656486
1112	15	-0.43708478834182785
1112	16	-0.4037581331134774
1112	17	-0.4537564955971618
1112	18	-0.4592494271265437
1112	19	-0.36158688443260445
1112	20	-0.3852756718269035
1112	21	-0.3451881630388627
1112	22	-0.29696726621083513
1112	23	-0.3480866714402353
1112	24	-0.4393694294403616
1112	25	-0.49236421587688994
1113	1	-0.6136417061879946
1113	2	-0.576930409065529
1113	3	-0.5920164782687003
1113	4	-0.5080819689521121
1113	5	-0.4908740736614945
1113	6	-0.6652377917506342
1113	7	-0.5941857111594118
1113	8	-0.5648973484491364
1113	9	-0.5238870012965222
1113	10	-0.6295518643395184
1113	11	-0.6613217804022611
1113	12	-0.5322701778102328
1113	13	-0.6367775390690937
1113	14	-0.7725681199666994
1113	15	-0.8323038460494345
1113	16	-0.772275607905911
1113	17	-0.874631512193325
1113	18	-0.7801493570651274
1113	19	-0.9473256877611362
1113	20	-1.0139461476527103
1113	21	-0.9729637922798418
1113	22	-0.9941788878855888
1113	23	-1.1458930950952464
1113	24	-1.1864947460573347
1113	25	-1.22180398976883
1114	1	-0.9157143223352016
1114	2	-0.839268878868836
1114	3	-0.9557452486631419
1114	4	-0.9672716509567962
1114	5	-0.9528321226144598
1114	6	-1.0959606866188807
1114	7	-0.9809517487797095
1114	8	-1.0523313991189909
1114	9	-1.193897407703787
1114	10	-1.023605310401905
1114	11	-1.1170795673204452
1114	12	-1.2869116164567558
1114	13	-1.2095296831112918
1114	14	-1.2570724589417992
1114	15	-1.3706248555398872
1114	16	-1.2921918177762777
1114	17	-1.602971856986784
1114	18	-1.2735325293513213
1114	19	-1.2893658312987597
1114	20	-1.1831917682683368
1114	21	-1.2562535461283701
1114	22	-1.2440094978269514
1114	23	-1.5331263333115928
1114	24	-1.424302431743449
1114	25	-1.4731115509322663
1115	1	-1.2441210838693202
1115	2	-1.2123813110490396
1115	3	-1.3479531412572854
1115	4	-1.3557082396718911
1115	5	-1.2199193857099502
1115	6	-1.3123946771164938
1115	7	-1.4231292758541656
1115	8	-1.402062594020876
1115	9	-2.0106629968667775
1115	10	-1.7090096093912521
1115	11	-1.707348371472449
1115	12	-1.6905774901539843
1115	13	-1.7083438714866972
1115	14	-1.9934143850060408
1115	15	-2.064632095580478
1115	16	-2.091575807867059
1115	17	-2.0093006623460212
1115	18	-1.876151003129877
1115	19	-1.8706586890674046
1115	20	-1.9219315958902001
1115	21	-2.090111081649738
1115	22	-2.1723064013670665
1115	23	-2.1088713931315226
1115	24	-2.250625638001722
1115	25	-2.1596626816611533
1116	1	-1.7297826917818284
1116	2	-1.7742856639108524
1116	3	-1.6643476701286737
1116	4	-1.9477457111668173
1116	5	-1.6341574898050055
1116	6	-1.811378331101043
1116	7	-1.7486757551213579
1116	8	-1.7397866347249398
1116	9	-2.1802241114455967
1116	10	-2.8277929340843926
1116	11	-2.3738344253939587
1116	12	-2.000094484760268
1116	13	-1.9941176184391836
1116	14	-2.211124273669199
1116	15	-2.8895231289474523
1116	16	-2.4833750116328446
1116	17	-2.4020170688634863
1116	18	-2.419847825380849
1116	19	-2.348880470219065
1116	20	-2.407085574319254
1116	21	-2.33703821727417
1116	22	-2.367760856872877
1116	23	-2.439237823439818
1116	24	-2.3049300351973727
1116	25	-2.3935657334367875
1201	1	1.142540643719613
1201	2	1.2444347417907196
1201	3	1.11917884513095
1201	4	1.1843946019720661
1201	5	1.1573176397932698
1201	6	1.1242746474436984
1201	7	1.118381391298037
1201	8	1.2023089292660387
1201	9	1.3754037811384237
1201	10	1.3808884907380872
1201	11	1.417656086254489
1201	12	1.3536382010448307
1201	13	1.3492124151639828
1201	14	1.6676071878809324
1201	15	1.6345489383610357
1201	16	1.6089679720342431
1201	17	1.541640043607238
1201	18	1.6741074194626364
1201	19	1.6464022876142863
1201	20	1.688991341940202
1201	21	1.7502310331963784
1201	22	1.764122708217677
1201	23	1.6834240223160748
1201	24	1.7366490584333505
1201	25	1.8393855622455746
1202	1	0.9963952372674321
1202	2	1.1070440426395578
1202	3	0.9818048830500885
1202	4	1.10294213546198
1202	5	0.9830592959642246
1202	6	1.033131459284128
1202	7	0.9068868928739704
1202	8	1.025100403452896
1202	9	1.0957105152860436
1202	10	1.141783629628435
1202	11	1.1175849326792502
1202	12	1.1249060761728273
1202	13	1.194867652542321
1202	14	1.2923242241579018
1202	15	1.3495440917596468
1202	16	1.2634737553648776
1202	17	1.2596866004176093
1202	18	1.3418970958862886
1202	19	1.247874813376967
1202	20	1.2886026466116576
1202	21	1.3344407848527382
1202	22	1.3535650231479437
1202	23	1.310230374833253
1202	24	1.293334403614395
1202	25	1.4415953601601714
1203	1	0.751018984342128
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2211	6	-0.3708426840647192
2211	7	-0.552441975493899
2211	8	-0.45874906658035997
2211	9	-0.4137122971584333
2211	10	-0.5236447049344264
2211	11	-0.5187034869743314
2211	12	-0.5804010628546976
2211	13	-0.5218033484714866
2211	14	-0.6565167951582609
2211	15	-0.5950811604524944
2211	16	-0.5849632575564954
2211	17	-0.636208406095335
2211	18	-0.6323107558409475
2211	19	-0.740154997569455
2211	20	-0.7499346659356689
2211	21	-0.807087603486039
2211	22	-0.6620401719274085
2211	23	-0.7510052994383543
2211	24	-0.6962102507380195
2211	25	-0.5460009040883733
2212	1	-0.47905483273017424
2212	2	-0.5103969595303077
2212	3	-0.42737347292329986
2212	4	-0.474987073405849
2212	5	-0.521606276439492
2212	6	-0.5039551703909027
2212	7	-0.4067738778057901
2212	8	-0.4503268079078637
2212	9	-0.6489194186334757
2212	10	-0.7562697688687408
2212	11	-0.7197217531899796
2212	12	-0.6626580842000891
2212	13	-0.5603738378053862
2212	14	-0.6735564891115899
2212	15	-0.8164351924121362
2212	16	-0.8222871274861845
2212	17	-0.8249567672964915
2212	18	-0.955565067412472
2212	19	-0.906883602151531
2212	20	-0.9281430135351201
2212	21	-0.9038242977399094
2212	22	-0.6887352942788283
2212	23	-0.7326594133806523
2212	24	-0.8222096104612717
2212	25	-0.9739684592681428
2213	1	-1.010471865958067
2213	2	-0.8513046331328766
2213	3	-0.9862911846151867
2213	4	-0.957489591826068
2213	5	-0.8882167720073895
2213	6	-0.8891184498220994
2213	7	-0.9374060664257726
2213	8	-1.0866157274309174
2213	9	-1.0780251744868607
2213	10	-1.1626134040502265
2213	11	-1.1255613865461314
2213	12	-1.1490169474821244
2213	13	-1.2120874503671715
2213	14	-1.3500563140138553
2213	15	-1.3005384663902007
2213	16	-1.4306987082151323
2213	17	-1.3405261272904454
2213	18	-1.2368155258126166
2213	19	-1.294759034255236
2213	20	-1.3390197415527254
2213	21	-1.3216755720764297
2213	22	-1.3342571459039974
2213	23	-1.3393680399470824
2213	24	-1.3320759323983182
2213	25	-1.2266981344252983
2214	1	-1.1839841286849762
2214	2	-1.2588475957740854
2214	3	-1.232593941403973
2214	4	-1.2670733702894168
2214	5	-1.2714808143790322
2214	6	-1.309840119626564
2214	7	-1.377866237664641
2214	8	-1.3184074825829413
2214	9	-1.4560352471431983
2214	10	-1.6859596613435102
2214	11	-1.6823583535038367
2214	12	-1.615102075083314
2214	13	-1.6154553334101678
2214	14	-1.7791209014779668
2214	15	-1.8179831925186178
2214	16	-1.8813215483433794
2214	17	-1.8081590406929067
2214	18	-1.7937967688730723
2214	19	-1.7899973778216705
2214	20	-1.8274229888753055
2214	21	-1.7756596468743382
2214	22	-1.6792479117088632
2214	23	-1.9156091262764576
2214	24	-1.8369438145145054
2214	25	-1.6739777572227936
2215	1	-1.7046924019894156
2215	2	-1.4644933033294798
2215	3	-1.648285212440591
2215	4	-2.2023554131465097
2215	5	-1.706208076111153
2215	6	-1.653610979722636
2215	7	-1.621675963810297
2215	8	-1.7234465699175558
2215	9	-1.8598486036182322
2215	10	-1.9024131519351286
2215	11	-1.8910583254100501
2215	12	-1.9845427953808183
2215	13	-1.9884816675839687
2215	14	-2.1198800408997442
2215	15	-2.225044419650061
2215	16	-2.151268005087367
2215	17	-2.1106637411296845
2215	18	-2.4022051754263316
2215	19	-2.83971261993959
2215	20	-2.4273449334152417
2215	21	-2.305029117734769
2215	22	-3.199751912624315
2215	23	-2.469744736019038
2215	24	-2.457420894428516
2215	25	-2.353921268486829
2216	1	-3.1062944428975356
2216	2	-5.883734945789808
2216	3	-3.6232635435232012
2216	4	-6.239445005723372
2216	5	-3.8540146373511135
2216	6	-2.719254202830083
2216	7	-2.584146234548811
2216	8	-2.460493132894591
2216	9	-2.8082361672008247
2216	10	-2.758606886783383
2216	11	-2.525005021951003
2216	12	-2.7425071870741107
2216	13	-2.7666038262570254
2216	14	-3.419754682884843
2216	15	-3.224006695298968
2216	16	-3.385481363679837
2216	17	-3.062812478971466
2216	18	-2.9733763755594538
2216	19	-3.076590498483057
2216	20	-3.464793357145055
2216	21	-3.6603815309793006
2216	22	-3.6389683674939945
2216	23	-3.489299435599783
2216	24	-3.5367854819948477
2216	25	-3.826576558284998
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	140296.0
3	10.0
4	935.0
5	5816.0
6	7457.0
7	7172.0
8	6541.0
9	5927.0
10	5432.0
11	5348.0
12	5039.0
13	4916.0
14	4900.0
15	5200.0
16	5228.0
17	5517.0
18	6183.0
19	7490.0
20	9670.0
21	12597.0
22	15565.0
23	18687.0
24	21371.0
25	24507.0
26	27326.0
27	30626.0
28	35145.0
29	41788.0
30	52112.0
31	66691.0
32	85134.0
33	112649.0
34	152431.0
35	212124.0
36	314855.0
37	552912.0
38	1628397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1145339463077	17.829827138693304	12.37927692503134	25.676361989967656
2	16.201348808576384	20.68384158064421	38.322843306391626	24.79196630438779
3	18.392660453059545	24.914266155894595	29.05395840982381	27.639114981222047
4	12.653674713802749	16.743759209146365	37.77275594065114	32.82981013639974
5	15.247235578385471	35.74847607006925	33.672242519165955	15.33204583237932
6	31.956972845738708	37.03027021526298	17.002384805382846	14.010372133615471
7	28.360099590056702	31.084388799492867	22.0940684618027	18.46144314864773
8	26.2337938169476	33.22121991238339	20.44391273073614	20.10107353993287
9	26.975528676429317	15.297045130331318	19.31732807733136	38.410098115908006
10	15.908130082874372	26.707484960650362	31.91029671143057	25.474088245044697
11	35.259315425748284	22.549183086377454	23.252456354443975	18.939045133430284
12	24.414397373526867	24.279749697464453	29.094130036083033	22.211722892925646
13	28.91794790394025	20.729192188632933	25.240987277685818	25.111872629741
14	23.316763335741303	20.633270018131675	25.83506371530063	30.214902930826394
15	24.41158657156843	27.39216407488018	23.132295881258106	25.06395347229328
16	24.766159237373817	26.794871653057296	24.117594028543174	24.321375081025714
17	24.217431176422437	25.989874753589138	25.34254152202819	24.45015254796024
18	25.02762209380311	25.11592982397916	26.209999820231676	23.646448261986052
19	25.995015037307013	25.334225375407186	25.347473251183594	23.323286336102203
20	25.06672455776227	25.366393942452746	25.383622996223714	24.18325850356127
21	25.4280776694411	25.23889025166268	25.279653627456483	24.05337845143973
22	24.707823555490776	25.18443823880738	25.628196295664278	24.47954191003756
23	24.078138736383394	24.737609583413647	25.78678409029001	25.39746758991295
24	24.14701632362397	25.706921465461814	25.63720209904796	24.508860111866255
25	24.401224722671557	25.230492839974346	25.718982510006665	24.64929992734743
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17752.0
1	17752.0
2	14412.0
3	11072.0
4	11072.0
5	11072.0
6	8693.5
7	6315.0
8	6315.0
9	6315.0
10	8212.5
11	10110.0
12	10110.0
13	10110.0
14	14063.0
15	18016.0
16	18016.0
17	18016.0
18	30186.5
19	42357.0
20	42357.0
21	42357.0
22	60936.0
23	79515.0
24	79515.0
25	79515.0
26	107954.0
27	136393.0
28	136393.0
29	136393.0
30	165176.0
31	193959.0
32	193959.0
33	193959.0
34	229093.0
35	264227.0
36	264227.0
37	264227.0
38	292358.5
39	320490.0
40	320490.0
41	320490.0
42	351745.5
43	383001.0
44	383001.0
45	383001.0
46	406151.0
47	429301.0
48	429301.0
49	429301.0
50	432913.0
51	436525.0
52	436525.0
53	436525.0
54	409410.0
55	382295.0
56	382295.0
57	382295.0
58	348886.0
59	315477.0
60	315477.0
61	315477.0
62	274825.5
63	234174.0
64	234174.0
65	234174.0
66	193892.0
67	153610.0
68	153610.0
69	153610.0
70	120784.5
71	87959.0
72	87959.0
73	87959.0
74	70696.5
75	53434.0
76	53434.0
77	53434.0
78	44462.5
79	35491.0
80	35491.0
81	35491.0
82	27076.0
83	18661.0
84	18661.0
85	18661.0
86	14070.0
87	9479.0
88	9479.0
89	9479.0
90	6708.0
91	3937.0
92	3937.0
93	3937.0
94	2151.5
95	366.0
96	366.0
97	366.0
98	222.0
99	78.0
100	78.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.41523119961229354
2	0.7253305027395764
3	0.740561043733881
4	0.7504128711518187
5	0.759770735078049
6	0.7619661283745253
7	0.7785962325953336
8	0.768744405177396
9	0.7562306633874809
10	0.74958959866564
11	0.7618289162934956
12	0.7653689879840636
13	0.7636675581792944
14	0.7744798701644404
15	0.7706928167280188
16	0.7644908306654731
17	0.7706379318956068
18	0.7746719670778821
19	0.7771692269526239
20	0.7686346355125722
21	0.7684699810153365
22	0.7762087423854156
23	0.7660001635568006
24	0.7702811804849294
25	0.7726412282786416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3643994.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.96607823702795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48707611204499	49.268295442905455
2	7.998730420255712	9.113126058343646
3	2.092253369792776	3.5756240746590193
4	0.913883080020058	2.0824134014367477
5	0.5408341086273639	1.5404599072659841
6	0.3414979940421825	1.1672280867837044
7	0.2420936938504181	0.9653789813201805
8	0.18462328024877	0.8413811381622536
9	0.14040615988567656	0.7198549460107296
>10	0.8798219255175601	9.662581822595183
>50	0.09110658488066523	3.605282592751704
>100	0.07282211422457406	8.380718484382928
>500	0.009706638311094237	3.7599157602844544
>1k	0.004998918723766357	4.5913576958989015
>5k	9.706638298575451E-5	0.37393184019216613
>10k+	4.853319149287726E-5	0.35244976700700437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	12748	0.3498359217935046	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7822	0.214654579562974	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5703	0.156504099622557	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4728	0.12974774382175164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.23272486178627E-5
2	0.0	0.0	0.0	0.0	8.23272486178627E-5
3	0.0	0.0	0.0	0.0	8.23272486178627E-5
4	0.0	0.0	0.0	0.0	8.23272486178627E-5
5	0.0	0.0	0.0	0.0	8.23272486178627E-5
6	0.0	0.0	0.0	0.0	8.23272486178627E-5
7	0.0	0.0	0.0	0.0	8.23272486178627E-5
8	0.0	0.0	0.0	0.0	1.0976966482381694E-4
9	0.0	0.0	0.0	0.0	1.0976966482381694E-4
10	2.7442416205954235E-5	0.0	0.0	0.0	1.372120810297712E-4
11	2.7442416205954235E-5	0.0	0.0	0.0	1.372120810297712E-4
12	2.7442416205954235E-5	0.0	0.0	0.0	1.9209691344167966E-4
13	2.7442416205954235E-5	0.0	0.0	0.0	1.9209691344167966E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1350	0.0	15.282378	1
GTATCAA	4995	0.0	13.685427	1
AACCGCG	360	0.0	11.3465605	7
GTATTAC	255	0.0	10.812416	1
CTCGACG	115	8.8319575E-7	10.738653	13
GTATTAG	605	0.0	10.686082	1
GCGTTAT	280	0.0	10.526127	1
GCTCGTA	290	0.0	10.481858	9
CGTTATT	265	0.0	10.3967705	2
CGAGCCG	620	0.0	10.112454	15
TACCGTG	85	6.6009857E-4	10.058237	7
ATACCGT	435	0.0	10.045531	6
AGAACCG	460	0.0	9.912877	5
GTATAAA	510	0.0	9.880311	1
ACCGTCC	185	6.511982E-10	9.756054	8
GCCGCCT	635	0.0	9.723978	18
CTAGAAC	255	0.0	9.686111	3
TAGAACC	560	0.0	9.499841	4
ATCCGTG	130	4.245665E-6	9.499446	7
CTCGTAG	340	0.0	9.499315	10
>>END_MODULE