FastQCFastQC Report
Thu 2 Feb 2017
SRR4050260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643994
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132890.3646822689609258No Hit
TATCAACGCAGAGTACTTTTTTTTT90800.24917713915006445No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49340.13540088156017818No Hit
GGTATCAACGCAGAGTACTTTTTTT47890.13142173121031483No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42770.11737121411286627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.016.0526751
GTATCAA56050.013.4541141
CGTTATT2650.012.2067682
AACCGCG3050.011.82630357
CTCGACG1157.158815E-811.55647113
CGTCGTA4450.011.51921410
CGTCTTA1001.9500349E-611.39106715
ATACCGT5050.011.2821546
GCTCGTA3200.011.2725649
TATAGCG600.00581369811.0997822
TACCGTC4850.010.9600257
ACGCTTA1053.503099E-610.84848617
GTATTAG5400.010.7830021
GCGTTAT3100.010.77731
GTCGTAG4850.010.56932111
CGAGCCG5350.010.46842615
CTCGTAG3600.010.28374310
CGATTAG1201.5407586E-610.28360214
CCGTCGT5400.010.19584759
GCCGCCT5750.010.07008618
Produced by FastQC (version 0.11.5)