FastQCFastQC Report
Thu 2 Feb 2017
SRR4050259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846991
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110770.3890774505433983No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN66380.23315844693572968No Hit
TATCAACGCAGAGTACTTTTTTTTT64510.2265901086445303No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN50350.17685338661063557No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43660.1533548929378421No Hit
GGTATCAACGCAGAGTACTTTTTTT39660.13930497145934076No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31030.10899226586947412No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29680.10425041737047991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.015.7786511
TTAGACG456.7598827E-414.77918153
GTATCAA53050.013.074971
TATAACG853.9400184E-612.2959382
AACCGCG802.8707122E-511.8740277
CGGTTCT1950.011.20460612
GGAGCGT855.3268137E-511.1763456
ATAGGCG600.00587352211.0843865
GCGCGCT1502.1373125E-910.766355515
GTCCTAG2850.010.6686241
CGCGGTT2001.8189894E-1210.44932810
CGCGCTA1553.6689016E-910.41905416
ATACCGT2750.010.3635216
CGCAGAT1659.313226E-1010.3627878
TAGGACC8600.010.2744134
ACGTTCC2800.010.17827816
CGACCGA1409.701762E-810.17827818
TAGACGT750.002646245310.1344754
ACTGTTC11350.010.043238
GATATAC10750.09.9878791
Produced by FastQC (version 0.11.5)