##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050259_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2846991
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.884839467353427	31.0	30.0	34.0	26.0	34.0
2	29.988482928116035	31.0	31.0	34.0	26.0	34.0
3	30.109884787131396	31.0	31.0	34.0	26.0	34.0
4	33.474291629302655	37.0	35.0	37.0	28.0	37.0
5	33.579134250863454	37.0	35.0	37.0	28.0	37.0
6	33.647794460888704	37.0	35.0	37.0	29.0	37.0
7	33.528584389623994	37.0	35.0	37.0	28.0	37.0
8	33.47758176966489	37.0	35.0	37.0	28.0	37.0
9	34.913224172468404	39.0	35.0	39.0	27.0	39.0
10	34.81910796346037	39.0	35.0	39.0	27.0	39.0
11	34.82970476548749	39.0	35.0	39.0	27.0	39.0
12	34.73469392772931	39.0	35.0	39.0	27.0	39.0
13	34.72609080955999	39.0	35.0	39.0	27.0	39.0
14	35.58150306762473	40.0	36.0	41.0	25.0	41.0
15	35.72641044527362	40.0	36.0	41.0	27.0	41.0
16	35.75060195132335	40.0	36.0	41.0	27.0	41.0
17	35.76266556515282	40.0	36.0	41.0	27.0	41.0
18	35.60277324375103	40.0	36.0	41.0	26.0	41.0
19	35.746270009283485	40.0	36.0	41.0	27.0	41.0
20	35.72010940673855	40.0	36.0	41.0	27.0	41.0
21	35.686447902364286	40.0	36.0	41.0	27.0	41.0
22	35.62331563394475	40.0	36.0	41.0	27.0	41.0
23	35.529702060877604	40.0	36.0	41.0	26.0	41.0
24	35.46940717410065	40.0	36.0	41.0	26.0	41.0
25	35.2057045491187	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1310670312976399
1101	2	1.050710511157341
1101	3	0.9646147962930556
1101	4	1.0943354799486968
1101	5	1.0595208027268228
1101	6	1.0672356785392196
1101	7	1.1001052995702878
1101	8	1.1188455062026677
1101	9	1.2674695777181597
1101	10	1.2221091312211456
1101	11	1.2461486578352492
1101	12	1.2386362655613254
1101	13	1.3074845369307653
1101	14	1.8015493670631955
1101	15	1.6191600389883405
1101	16	1.508715681855854
1101	17	1.5125227891757191
1101	18	1.6000715893925133
1101	19	1.535743259962409
1101	20	1.5210771950191884
1101	21	1.577224237273434
1101	22	1.55132100600661
1101	23	1.4749204331935957
1101	24	1.6325099045928582
1101	25	1.5984540863735575
1102	1	1.0860733562951381
1102	2	1.0110735106489912
1102	3	0.9340687913035204
1102	4	1.1278480401857678
1102	5	1.027238429337217
1102	6	0.9894859543840511
1102	7	1.0629063031812933
1102	8	1.0552915147154138
1102	9	1.098853449142311
1102	10	1.246766415342158
1102	11	1.2273898113671535
1102	12	1.2278637071026353
1102	13	1.2622785606749503
1102	14	1.6493332132097294
1102	15	1.5802566906543447
1102	16	1.4495831767933396
1102	17	1.4836800176524108
1102	18	1.576511532954072
1102	19	1.4781344725220293
1102	20	1.3950192539037403
1102	21	1.4398658157154216
1102	22	1.5436550223576475
1102	23	1.5042569431930701
1102	24	1.5347093511695604
1102	25	1.5054543234910582
1103	1	0.7871791495063576
1103	2	0.8735714795709804
1103	3	0.7858767391009671
1103	4	0.838529786980196
1103	5	0.7701198551196455
1103	6	0.7130383183254949
1103	7	0.6609313452647001
1103	8	0.7797500544875007
1103	9	0.8715602678904091
1103	10	0.9501177457324275
1103	11	0.9380683577760465
1103	12	1.020826632703077
1103	13	1.025002176779907
1103	14	1.3338465409152747
1103	15	1.211562808664695
1103	16	1.145458406562689
1103	17	1.1740328488234724
1103	18	1.2143960434414396
1103	19	1.2778689472017462
1103	20	1.1270288366581482
1103	21	1.0768519513118946
1103	22	1.143054029958094
1103	23	1.0800621751966872
1103	24	1.15184916261218
1103	25	1.1732675060524542
1104	1	0.5851569511489885
1104	2	0.5770927757889517
1104	3	0.5234107582094545
1104	4	0.6563605352848825
1104	5	0.6334316051360531
1104	6	0.6072093812542221
1104	7	0.6142255734565936
1104	8	0.5596679976728893
1104	9	0.6120146543085383
1104	10	0.6355887302763747
1104	11	0.6788766074106434
1104	12	0.720733934070239
1104	13	0.7122593581421057
1104	14	1.0876236846011906
1104	15	0.924322293077708
1104	16	0.861300680047556
1104	17	0.8400175843390087
1104	18	0.9775850509085373
1104	19	0.8843578135661261
1104	20	0.890417631111859
1104	21	0.8606006835180509
1104	22	0.8304019373727627
1104	23	0.9189162046283457
1104	24	0.8818922445262345
1104	25	0.9674299452644135
1105	1	0.5328696610346135
1105	2	0.603470516549546
1105	3	0.5487838782182806
1105	4	0.673379244952244
1105	5	0.5499734887190115
1105	6	0.6538006623885266
1105	7	0.6224558603077739
1105	8	0.6252341027257344
1105	9	0.7168494741256026
1105	10	0.7468549457811378
1105	11	0.7010418247706625
1105	12	0.7038976319668535
1105	13	0.6308568946206208
1105	14	0.9262148170271658
1105	15	0.9101423535924056
1105	16	0.9246264460453943
1105	17	0.8942146972386169
1105	18	0.9699346066423828
1105	19	0.8763089748702981
1105	20	0.8903492732476437
1105	21	0.9100850613475515
1105	22	0.8183623185937776
1105	23	0.8098649038501478
1105	24	0.8883862416264208
1105	25	0.7942835527007901
1106	1	0.6078001446972046
1106	2	0.5152824474068147
1106	3	0.5158794785114615
1106	4	0.7477966623953591
1106	5	0.5697603338038135
1106	6	0.5282811079775556
1106	7	0.48783686687582417
1106	8	0.6283424455418114
1106	9	0.7018091332602623
1106	10	0.6313346213430435
1106	11	0.6046646597823653
1106	12	0.6751034659204862
1106	13	0.7909560855217492
1106	14	1.0484854273734783
1106	15	0.9174867011655365
1106	16	0.8485691560275086
1106	17	0.8272676836604731
1106	18	0.991756604891286
1106	19	0.9189764628992307
1106	20	0.8483363082724722
1106	21	0.8585591242673232
1106	22	0.8120560816592857
1106	23	0.9389140344324858
1106	24	0.9071797517237599
1106	25	0.9183220882803269
1107	1	0.2764607716292993
1107	2	0.3516477008952883
1107	3	0.3335052626753203
1107	4	0.36366006763860526
1107	5	0.24291410431550986
1107	6	0.2787901962569279
1107	7	0.3223047493029867
1107	8	0.2643790297419102
1107	9	0.34486048762876464
1107	10	0.36165772968670495
1107	11	0.32373387899746575
1107	12	0.30765535768072994
1107	13	0.27819542143356557
1107	14	0.6023088054520471
1107	15	0.5070829965290073
1107	16	0.4697433050325657
1107	17	0.3287252496624049
1107	18	0.4433142928350051
1107	19	0.346925488403933
1107	20	0.3895734979422443
1107	21	0.4266590849500318
1107	22	0.5802532965320282
1107	23	0.5256932832145225
1107	24	0.5312450932397468
1107	25	0.49145715606317
1108	1	0.351729733932892
1108	2	0.35227791374594375
1108	3	0.2968046949589791
1108	4	0.3780125240684242
1108	5	0.2487704411675722
1108	6	0.31291165175142055
1108	7	0.26914801693600054
1108	8	0.32859075255770875
1108	9	0.3612094480881396
1108	10	0.35367026775771393
1108	11	0.4187234324791618
1108	12	0.45986549631631135
1108	13	0.44099226847369977
1108	14	0.8447852479736042
1108	15	0.5655377685961298
1108	16	0.5701137913652943
1108	17	0.3899440906330227
1108	18	0.6131473118651414
1108	19	0.5280998219641546
1108	20	0.5033289255560405
1108	21	0.556523843274995
1108	22	0.46497896438013697
1108	23	0.4820884335796478
1108	24	0.4142539411197248
1108	25	0.4916122326555268
1109	1	0.2205658458598201
1109	2	0.2859029232987993
1109	3	0.3213524027411445
1109	4	0.43537840110567316
1109	5	0.3643857799905206
1109	6	0.34986385205255033
1109	7	0.33844295173589245
1109	8	0.3704141043222009
1109	9	0.3726927315254187
1109	10	0.3469343889944483
1109	11	0.37267451584299494
1109	12	0.43900088913859747
1109	13	0.45801316968419314
1109	14	0.6695843496045839
1109	15	0.5815370951358858
1109	16	0.5335814827071061
1109	17	0.5239473651241795
1109	18	0.6538015525639835
1109	19	0.5575060206470752
1109	20	0.6059444701641823
1109	21	0.6035874427433967
1109	22	0.5758955498039384
1109	23	0.5633925246209373
1109	24	0.5341132230503973
1109	25	0.514606571086766
1110	1	-0.1970477357685958
1110	2	-0.20838047319109876
1110	3	-0.23856343795788604
1110	4	-0.21023158187018254
1110	5	-0.2619616435376315
1110	6	-0.23345252649841797
1110	7	-0.23561173244660694
1110	8	-0.26937682394442675
1110	9	-0.2976808671040203
1110	10	-0.38620359827251605
1110	11	-0.40279180810586723
1110	12	-0.33649462400944685
1110	13	-0.3116153144272502
1110	14	-0.11468728643172454
1110	15	-0.1763473121673158
1110	16	-0.2939620814501751
1110	17	-0.37028735380021516
1110	18	-0.14512835983374117
1110	19	-0.25714583987055306
1110	20	-0.40719384849170126
1110	21	-0.2740620310032753
1110	22	-0.3452601363291308
1110	23	-0.18408785985187848
1110	24	-0.23999141993969175
1110	25	-0.3144514597385708
1111	1	-0.25797587572224856
1111	2	-0.2995457552434857
1111	3	-0.3267326084089248
1111	4	-0.15313381949295035
1111	5	-0.298775165408081
1111	6	-0.33310709034491737
1111	7	-0.3815779672284023
1111	8	-0.4085615874480055
1111	9	-0.48913858614928074
1111	10	-0.38213351383256366
1111	11	-0.4569229626446045
1111	12	-0.39493619759129217
1111	13	-0.5017837459390577
1111	14	-0.3067604593506843
1111	15	-0.4635589106435205
1111	16	-0.43862073457201944
1111	17	-0.46666475804301655
1111	18	-0.3602756275521415
1111	19	-0.5453185077281333
1111	20	-0.5348712477125872
1111	21	-0.4514565886799957
1111	22	-0.5009409109957161
1111	23	-0.5425323927777157
1111	24	-0.6833051857318253
1111	25	-0.681592338861023
1112	1	-0.4738017971894877
1112	2	-0.5113790894234072
1112	3	-0.558782855019615
1112	4	-0.4202799928366474
1112	5	-0.5050511662004808
1112	6	-0.5476681267024333
1112	7	-0.5457235835180896
1112	8	-0.4055932853452475
1112	9	-0.5119997273142189
1112	10	-0.45643816988980745
1112	11	-0.3847124233181134
1112	12	-0.4680450500092306
1112	13	-0.5938511443952237
1112	14	-0.4861426881424151
1112	15	-0.5293287880747073
1112	16	-0.7030337029249125
1112	17	-0.7722446952940132
1112	18	-0.6238226224235177
1112	19	-0.6390740846176755
1112	20	-0.5784643646245087
1112	21	-0.5246138658541568
1112	22	-0.6535910658364088
1112	23	-0.6899103446137076
1112	24	-0.5903360031789617
1112	25	-0.5093996625127915
1113	1	-0.8575570853243377
1113	2	-0.9897052257787387
1113	3	-0.9522083973502653
1113	4	-0.8731408829238134
1113	5	-0.95815061601526
1113	6	-1.0209147309709294
1113	7	-1.1070838488929482
1113	8	-1.079777688726736
1113	9	-1.098108098459086
1113	10	-1.1729813554110962
1113	11	-1.211270002795402
1113	12	-1.0611805212468894
1113	13	-1.1193938403758779
1113	14	-1.1515350878413813
1113	15	-1.1646491449279068
1113	16	-1.2418385661823308
1113	17	-1.169726483651715
1113	18	-1.1617035690635902
1113	19	-1.1926524805318337
1113	20	-1.3488785744547798
1113	21	-1.1682443576598303
1113	22	-1.23843539922089
1113	23	-1.6184786939849758
1113	24	-1.4926759343510057
1113	25	-1.6351020071932396
1114	1	-2.4132981328202234
1114	2	-2.587500505586398
1114	3	-2.2048369892082462
1114	4	-5.248044275036229
1114	5	-2.8287695679678144
1114	6	-2.3477287879906186
1114	7	-2.1268983529558056
1114	8	-2.3245183767095874
1114	9	-3.0214543634703546
1114	10	-2.7051563733118087
1114	11	-2.560068032208328
1114	12	-2.6570565729823983
1114	13	-2.5811616173363063
1114	14	-2.4895584376036055
1114	15	-3.240042326090702
1114	16	-3.179944889714889
1114	17	-2.815780483349158
1114	18	-2.7778313758482156
1114	19	-2.8396101189448544
1114	20	-3.139264008801085
1114	21	-3.1173309167683527
1114	22	-3.118293976104823
1114	23	-3.267630181267826
1114	24	-3.360040923616573
1114	25	-3.321664592937303
1115	1	-2.6988718572562433
1115	2	-2.1704846870854624
1115	3	-1.727508682656545
1115	4	-1.7703803632669484
1115	5	-1.7032098753944744
1115	6	-1.713661162243426
1115	7	-1.8159344610389496
1115	8	-1.7705944491660226
1115	9	-2.1530668319079354
1115	10	-1.8039262607718172
1115	11	-1.8756762904491424
1115	12	-2.2713279964632704
1115	13	-2.4394591423465144
1115	14	-2.747699136851452
1115	15	-2.5295915588817053
1115	16	-2.32406195194077
1115	17	-2.4748281101433918
1115	18	-2.2649620102489294
1115	19	-2.1727279424527097
1115	20	-2.2662012896205184
1115	21	-2.2870165750539755
1115	22	-2.266831920367558
1115	23	-2.31021308865526
1115	24	-2.504538965746697
1115	25	-2.7304813889716684
1116	1	-1.5793259850580412
1116	2	-1.6225623369067002
1116	3	-1.6564666724578139
1116	4	-1.6184767696904565
1116	5	-1.778018456311564
1116	6	-1.7708423939054825
1116	7	-2.0050706267955647
1116	8	-1.8659171947208684
1116	9	-2.19299081591258
1116	10	-2.534972650455181
1116	11	-2.339728491888934
1116	12	-2.3589610485224455
1116	13	-2.2826191544428127
1116	14	-3.0713492401727507
1116	15	-2.9000185234878018
1116	16	-2.994203249151454
1116	17	-2.618049864415795
1116	18	-2.602638574459192
1116	19	-2.4895062202700444
1116	20	-2.527374438337027
1116	21	-2.699874534125506
1116	22	-2.541232228184512
1116	23	-3.0540147273404443
1116	24	-3.092933843369437
1116	25	-3.02746912565064
1201	1	1.2173252537390944
1201	2	1.1775789692018606
1201	3	1.1630018861689386
1201	4	1.2858652603699454
1201	5	1.2313416725449358
1201	6	1.1435973176955798
1201	7	1.1568890454394634
1201	8	1.1984336784326999
1201	9	1.3937957894501238
1201	10	1.3299705978737464
1201	11	1.3168709210845364
1201	12	1.4606664922113026
1201	13	1.4883966398513522
1201	14	1.776734060134821
1201	15	1.6410025786998474
1201	16	1.5970138681428026
1201	17	1.6156788950958756
1201	18	1.73120851745702
1201	19	1.6667997813172306
1201	20	1.6432917583280684
1201	21	1.5640834428437316
1201	22	1.5905240529076679
1201	23	1.5195040290842954
1201	24	1.622498627694391
1201	25	1.6229552511303282
1202	1	0.8873802364512215
1202	2	0.914973507576466
1202	3	0.916667726672916
1202	4	0.9529156357797604
1202	5	0.8958278898014811
1202	6	0.7424365404685744
1202	7	0.7334541155420737
1202	8	0.8717819591564435
1202	9	0.9196775969001365
1202	10	1.0059081433313466
1202	11	0.9249753812440957
1202	12	0.9860529517218737
1202	13	1.0122804810043249
1202	14	1.279600196602722
1202	15	1.1874283527378253
1202	16	1.2749460230287255
1202	17	1.2170960531708204
1202	18	1.3180500816459428
1202	19	1.1190227976875633
1202	20	1.188659699112904
1202	21	1.1442335088621505
1202	22	1.147447384823657
1202	23	1.24098938754193
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2211	10	-0.17698176553611233
2211	11	-0.06362140382684345
2211	12	-0.04523215471774478
2211	13	-0.15939011965409833
2211	14	0.06357019695767008
2211	15	-0.07845883635111761
2211	16	-0.06704011408715616
2211	17	-0.14136676173338714
2211	18	0.016119449308533262
2211	19	-0.06076515498030943
2211	20	-0.06825455021935056
2211	21	-0.16620197737035625
2211	22	-0.1527057413687416
2211	23	-0.18655912922486095
2211	24	-0.0374923452709055
2211	25	-0.08186746325119998
2212	1	-0.28851391484379363
2212	2	-0.4141175230863148
2212	3	-0.3915296487865092
2212	4	-0.2903250075001367
2212	5	-0.39189524359972694
2212	6	-0.3337711191866646
2212	7	-0.37736316311477225
2212	8	-0.3989436719584276
2212	9	-0.5191673507298376
2212	10	-0.5489114949962683
2212	11	-0.6056354154359695
2212	12	-0.5244411124335642
2212	13	-0.515648616712788
2212	14	-0.5107039075851532
2212	15	-0.5709918325847667
2212	16	-0.6389371020899048
2212	17	-0.8214636417543772
2212	18	-0.5908466752087449
2212	19	-0.6263668930963462
2212	20	-0.6836089927580957
2212	21	-0.7487104500355741
2212	22	-0.8648441488329226
2212	23	-0.9703564444253558
2212	24	-0.7484121633931906
2212	25	-0.770170957046588
2213	1	-0.8480446227091285
2213	2	-0.6322786231842841
2213	3	-0.6233124617401913
2213	4	-0.5045710923804734
2213	5	-0.6465036689664814
2213	6	-0.7118182489344704
2213	7	-0.7316141777976455
2213	8	-0.7134575537392536
2213	9	-0.7226203014046604
2213	10	-0.8678051721067348
2213	11	-0.9010193655864782
2213	12	-0.782769658705817
2213	13	-1.0587401042389288
2213	14	-0.7680920313566162
2213	15	-0.9802928659632855
2213	16	-1.0517504108856315
2213	17	-1.0338921752843433
2213	18	-1.0201436979004654
2213	19	-1.0526893230712204
2213	20	-1.1502555363607314
2213	21	-1.055063628609318
2213	22	-1.0890540748649968
2213	23	-1.0985544971533514
2213	24	-1.1605657977647752
2213	25	-1.2098533607848836
2214	1	-1.2077895095293627
2214	2	-1.0361752813018015
2214	3	-1.1531712422678453
2214	4	-0.9489148105142959
2214	5	-0.9380575032479399
2214	6	-1.0495100922643275
2214	7	-0.963099841061414
2214	8	-1.078332050116117
2214	9	-1.0504853715034272
2214	10	-1.2365100419296553
2214	11	-1.1817659305277814
2214	12	-1.1503664313976643
2214	13	-1.4336651098585804
2214	14	-1.1417032190458443
2214	15	-1.3018207417778882
2214	16	-1.221176212345469
2214	17	-1.2746011021654056
2214	18	-1.2239534305987405
2214	19	-1.3550174661249343
2214	20	-1.4080649794422584
2214	21	-1.4251281622506013
2214	22	-1.450592404349905
2214	23	-1.4344007506455725
2214	24	-1.4753826434424724
2214	25	-1.4381546131046932
2215	1	-1.712343328854299
2215	2	-1.8393659417711135
2215	3	-1.6686142504395356
2215	4	-1.8879890979730334
2215	5	-1.7044448499476879
2215	6	-1.5856780872920808
2215	7	-1.5994741286199101
2215	8	-1.7210106805124887
2215	9	-2.0115491777293144
2215	10	-1.7295169865195916
2215	11	-1.8837382499370179
2215	12	-2.0637237680112435
2215	13	-2.005770065847578
2215	14	-2.9057751570563255
2215	15	-2.449517933314283
2215	16	-2.2684072454143305
2215	17	-2.2225091155298244
2215	18	-2.0502077516381263
2215	19	-2.305872635360032
2215	20	-2.607560371830047
2215	21	-2.38355675548479
2215	22	-2.6679223736324857
2215	23	-2.4010200292901303
2215	24	-2.4803482297619937
2215	25	-2.603891097791376
2216	1	-2.0227212023209127
2216	2	-1.959202471715777
2216	3	-2.0054438317375336
2216	4	-1.8625638137080003
2216	5	-2.444655969536324
2216	6	-2.0641275773251415
2216	7	-2.1225778769524233
2216	8	-2.5771431169822137
2216	9	-2.555720684867488
2216	10	-2.6285573502541055
2216	11	-2.6210452230418255
2216	12	-2.718794155823879
2216	13	-2.7448842522537262
2216	14	-2.547193573552228
2216	15	-2.6344183906133836
2216	16	-2.7932926380552487
2216	17	-2.805889348086829
2216	18	-3.0542191212650067
2216	19	-2.903571776791445
2216	20	-3.06954374224037
2216	21	-3.1963321312973463
2216	22	-3.429137095681746
2216	23	-3.209702378402376
2216	24	-3.319489134986199
2216	25	-3.3300063356054252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109888.0
3	6.0
4	792.0
5	4751.0
6	6578.0
7	6065.0
8	5656.0
9	5129.0
10	4655.0
11	4517.0
12	4180.0
13	3968.0
14	4031.0
15	3915.0
16	4105.0
17	4334.0
18	4998.0
19	6069.0
20	7914.0
21	10292.0
22	12861.0
23	15576.0
24	18185.0
25	20907.0
26	23302.0
27	25692.0
28	29006.0
29	33889.0
30	42250.0
31	53327.0
32	68555.0
33	89454.0
34	120241.0
35	167386.0
36	246098.0
37	429574.0
38	1248845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53635367550546	18.94220423292942	12.995034166511749	24.526407925053377
2	14.853655250407648	21.663132128089273	41.06758978417566	22.415622837327415
3	18.11749745568579	26.79680919305457	30.098830428795626	24.986862922464013
4	11.420099865221914	18.052306346240695	40.08164489386977	30.44594889466762
5	13.50894103040711	37.31226135292442	35.01039037753768	14.168407239130781
6	29.15481248518596	39.26242875993646	18.36399913680569	13.218759618071893
7	26.054479355074466	32.43068568087166	23.563490207717443	17.951344756336425
8	24.20401541986882	36.12229492586139	21.41169337694137	18.26199627732842
9	26.162538580328786	15.999119982965587	20.54578033954226	37.292561097163365
10	14.56861117369693	28.83763572034667	33.910411926306644	22.683341179649755
11	33.45059275255433	23.585697534368684	24.966917987382676	17.996791725694315
12	23.00955168994839	25.477115809155798	31.775507872371055	19.737824628524756
13	28.21183681178715	22.658596059583747	26.342682230634118	22.786884897994987
14	21.399731059006445	22.254548901352695	28.36061294778994	27.98510709185092
15	22.702032376397284	30.086839090359558	24.594198536140038	22.616929997103124
16	21.952621529018323	28.9340622256307	26.93989043976203	22.173425805588945
17	22.123007275278027	27.788470415862065	27.327765300740616	22.760757008119292
18	22.623841243251196	26.922630703233768	29.27749606673533	21.1760319867797
19	24.340692301380017	26.53190002292014	27.324377972897647	21.8030297028022
20	23.171215289286458	27.449112531318836	27.913107058327054	21.466565121067656
21	23.245529794238422	26.962297500589827	27.326769025721347	22.465403679450407
22	22.504161528867535	27.532059512869434	27.97739439125658	21.98638456700645
23	22.385367054648565	26.91242391055192	27.780112460982494	22.922096573817022
24	22.247924517088506	27.31426780415535	28.33195132891752	22.105856349838618
25	22.52286857651379	27.1268961058162	28.161508636331806	22.18872668133821
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11345.0
1	11345.0
2	10601.5
3	9858.0
4	9858.0
5	9858.0
6	8751.5
7	7645.0
8	7645.0
9	7645.0
10	9719.5
11	11794.0
12	11794.0
13	11794.0
14	16050.5
15	20307.0
16	20307.0
17	20307.0
18	33978.0
19	47649.0
20	47649.0
21	47649.0
22	69639.0
23	91629.0
24	91629.0
25	91629.0
26	125409.0
27	159189.0
28	159189.0
29	159189.0
30	195169.0
31	231149.0
32	231149.0
33	231149.0
34	250129.0
35	269109.0
36	269109.0
37	269109.0
38	284239.5
39	299370.0
40	299370.0
41	299370.0
42	314552.0
43	329734.0
44	329734.0
45	329734.0
46	330026.5
47	330319.0
48	330319.0
49	330319.0
50	319548.0
51	308777.0
52	308777.0
53	308777.0
54	276070.5
55	243364.0
56	243364.0
57	243364.0
58	214013.5
59	184663.0
60	184663.0
61	184663.0
62	153909.5
63	123156.0
64	123156.0
65	123156.0
66	99131.0
67	75106.0
68	75106.0
69	75106.0
70	57674.0
71	40242.0
72	40242.0
73	40242.0
74	32064.0
75	23886.0
76	23886.0
77	23886.0
78	19116.0
79	14346.0
80	14346.0
81	14346.0
82	11027.5
83	7709.0
84	7709.0
85	7709.0
86	6097.0
87	4485.0
88	4485.0
89	4485.0
90	3217.0
91	1949.0
92	1949.0
93	1949.0
94	1062.5
95	176.0
96	176.0
97	176.0
98	105.5
99	35.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2382515434716864
2	0.6737639845015316
3	0.6714808722612752
4	0.6807186956333898
5	0.6974381021928063
6	0.7123661437637141
7	0.7067812999760097
8	0.7021097010844081
9	0.6945227434860173
10	0.6941363706453586
11	0.699896838451544
12	0.7008100833476467
13	0.6946632427008024
14	0.6886217764650469
15	0.6957521116153862
16	0.7043576885209683
17	0.703198569998992
18	0.6962789836708301
19	0.6948739915229799
20	0.7031283203915994
21	0.6984215966963014
22	0.6991592175739227
23	0.7044279381283608
24	0.7087834137866962
25	0.6936797481973073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2846991.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.05544741609678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.35464413320562	61.49125732607512
2	5.909912389103867	8.044034636607941
3	1.3638060843398363	2.7844329977562774
4	0.6534802674721426	1.7789156792162901
5	0.3697598634788272	1.2582086472783223
6	0.2397489464524471	0.9789733091015468
7	0.18144089623395393	0.8643628960945526
8	0.11918001295680775	0.648867928386541
9	0.09850149154648857	0.6033206770514239
>10	0.6005916876570137	7.744116758870305
>50	0.0568965101283868	2.706372021747464
>100	0.04616040055524741	6.21949848885858
>500	0.003952885682130815	1.8033235205013443
>1k	0.0017163845724052003	2.040694217392879
>5k	1.5603496112774547E-4	0.6415309442175214
>10k+	5.20116537092485E-5	0.3920899508440457
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11077	0.3890774505433983	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6638	0.23315844693572968	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6451	0.2265901086445303	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5035	0.17685338661063557	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4366	0.1533548929378421	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3966	0.13930497145934076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3103	0.10899226586947412	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2968	0.10425041737047991	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.024960739250669E-5	0.0
6	0.0	0.0	0.0	7.024960739250669E-5	0.0
7	3.5124803696253345E-5	0.0	0.0	7.024960739250669E-5	0.0
8	3.5124803696253345E-5	0.0	0.0	7.024960739250669E-5	0.0
9	3.5124803696253345E-5	0.0	0.0	1.0537441108876002E-4	0.0
10	3.5124803696253345E-5	0.0	0.0	1.4049921478501338E-4	0.0
11	3.5124803696253345E-5	0.0	0.0	1.756240184812667E-4	0.0
12	3.5124803696253345E-5	0.0	0.0	1.756240184812667E-4	3.5124803696253345E-5
13	3.5124803696253345E-5	0.0	0.0	1.756240184812667E-4	3.5124803696253345E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1650	0.0	15.778651	1
TTAGACG	45	6.7598827E-4	14.7791815	3
GTATCAA	5305	0.0	13.07497	1
TATAACG	85	3.9400184E-6	12.295938	2
AACCGCG	80	2.8707122E-5	11.874027	7
CGGTTCT	195	0.0	11.204606	12
GGAGCGT	85	5.3268137E-5	11.176345	6
ATAGGCG	60	0.005873522	11.084386	5
GCGCGCT	150	2.1373125E-9	10.7663555	15
GTCCTAG	285	0.0	10.668624	1
CGCGGTT	200	1.8189894E-12	10.449328	10
CGCGCTA	155	3.6689016E-9	10.419054	16
ATACCGT	275	0.0	10.363521	6
CGCAGAT	165	9.313226E-10	10.362787	8
TAGGACC	860	0.0	10.274413	4
ACGTTCC	280	0.0	10.178278	16
CGACCGA	140	9.701762E-8	10.178278	18
TAGACGT	75	0.0026462453	10.134475	4
ACTGTTC	1135	0.0	10.04323	8
GATATAC	1075	0.0	9.987879	1
>>END_MODULE