FastQCFastQC Report
Thu 2 Feb 2017
SRR4050259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846991
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT173310.6087479728597667No Hit
TATCAACGCAGAGTACTTTTTTTTT117630.413173065879028No Hit
GTACTTTTTTTTTTTTTTTTTTTTT68290.2398672844417141No Hit
GGTATCAACGCAGAGTACTTTTTTT64150.2253256157114652No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53930.18942806633389428No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47210.16582419825001202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.016.4067841
GTATCAA61000.013.5931131
TGCGCGA604.0993802E-412.66465211
ATAGGCG550.003068332312.0915365
AACCGTG550.003076821612.0870767
AATTCCG1701.8189894E-1211.7359025
CGCGAGT658.037733E-411.69044913
CGGTCCA1701.2732926E-1111.17449710
ACACCGT951.3667755E-510.9959336
AACCGCG1303.285095E-810.9580637
AAGACCG700.00149141810.8577055
CGACCAT1502.1409505E-910.76476610
CGAATAC1002.398925E-510.45054154
CGAGCCG1409.7203156E-810.17695315
CCGATAA1606.197297E-910.0919689
CGCGTAT856.615794E-410.0556347
TCGATTA1252.5770369E-69.87842813
TCGCGTA1356.463488E-79.8501129
ACGAACG1651.0257281E-89.78632315
CGATAAC1651.0260919E-89.7861510
Produced by FastQC (version 0.11.5)