##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2846991
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8285056046893	31.0	31.0	34.0	27.0	34.0
2	30.840902552905856	31.0	31.0	34.0	27.0	34.0
3	31.052661213189644	31.0	31.0	34.0	27.0	34.0
4	34.63802449674059	37.0	35.0	37.0	31.0	37.0
5	34.55148295164965	37.0	35.0	37.0	32.0	37.0
6	34.50374061596963	37.0	35.0	37.0	31.0	37.0
7	34.43497819276563	37.0	35.0	37.0	31.0	37.0
8	34.413828845964034	37.0	35.0	37.0	31.0	37.0
9	35.987906178839346	39.0	37.0	39.0	31.0	39.0
10	35.89025255085106	39.0	37.0	39.0	31.0	39.0
11	35.86476283205672	39.0	37.0	39.0	30.0	39.0
12	35.79814653435856	39.0	37.0	39.0	30.0	39.0
13	35.7942631360619	39.0	37.0	39.0	30.0	39.0
14	36.953726934858594	40.0	37.0	41.0	31.0	41.0
15	36.94102475209792	40.0	37.0	41.0	31.0	41.0
16	36.938615190564356	40.0	37.0	41.0	31.0	41.0
17	36.93770124317218	40.0	37.0	41.0	31.0	41.0
18	36.9153270944657	40.0	37.0	41.0	31.0	41.0
19	36.98245726804194	40.0	37.0	41.0	31.0	41.0
20	36.94240972310766	40.0	37.0	41.0	31.0	41.0
21	36.901006009502666	40.0	37.0	41.0	31.0	41.0
22	36.847105241990576	40.0	37.0	41.0	31.0	41.0
23	36.758124981779005	40.0	37.0	41.0	31.0	41.0
24	36.70915398046569	40.0	37.0	41.0	31.0	41.0
25	36.503400256621816	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6869311756170333
1101	2	0.8894664716772525
1101	3	0.8532983171144011
1101	4	0.7479691746105672
1101	5	0.8453205467825669
1101	6	0.8414890621444115
1101	7	0.8314741271087271
1101	8	0.7931470999762098
1101	9	0.9748987142403109
1101	10	1.0289967441943944
1101	11	0.9459605904066706
1101	12	1.0240328336686915
1101	13	1.015153328472806
1101	14	1.1369553176312053
1101	15	1.2284948191315053
1101	16	1.1457524160341848
1101	17	1.187077578068923
1101	18	1.2146912885809087
1101	19	1.168769593042974
1101	20	1.2239305762167234
1101	21	1.1103731341171041
1101	22	1.1473552231499937
1101	23	1.187576495653559
1101	24	1.1497646498331875
1101	25	1.2301508905630953
1102	1	0.7060317173522286
1102	2	0.7755861080821731
1102	3	0.6557644789370265
1102	4	0.6325228009145007
1102	5	0.7276364465735767
1102	6	0.7794469743471808
1102	7	0.8055548493999538
1102	8	0.8293924533959256
1102	9	0.9235501224330562
1102	10	0.9861580981800628
1102	11	0.905175191811594
1102	12	0.9208745571773775
1102	13	0.9604329425752525
1102	14	0.9999917190823169
1102	15	1.0526981266969457
1102	16	1.0169434365510028
1102	17	0.94716751494758
1102	18	1.008941804339436
1102	19	1.0242844344175168
1102	20	0.963078675669621
1102	21	1.0954447978486925
1102	22	1.0968867343322657
1102	23	1.1871160319210716
1102	24	1.1473370615474607
1102	25	1.0796814510384038
1103	1	0.5756780807508051
1103	2	0.6650581931526887
1103	3	0.582988778557656
1103	4	0.5488811248805945
1103	5	0.5530356129931633
1103	6	0.6742577025462353
1103	7	0.6910953275095508
1103	8	0.6593515024627052
1103	9	0.695581126434675
1103	10	0.7412483280497568
1103	11	0.7042288511424175
1103	12	0.7989820065214417
1103	13	0.7553459030898964
1103	14	0.8931367132826225
1103	15	0.9274971774892649
1103	16	0.9842508981898845
1103	17	0.900844067849647
1103	18	0.9258058558955113
1103	19	0.8148442038510737
1103	20	0.8748425128198747
1103	21	0.8997964954165241
1103	22	0.9129671114816986
1103	23	0.9112482657175036
1103	24	0.94199252437555
1103	25	0.9725044731184624
1104	1	0.4441970003887654
1104	2	0.494976307042144
1104	3	0.4499637747104934
1104	4	0.506133980475191
1104	5	0.481124626441229
1104	6	0.476651618501414
1104	7	0.5500935753270966
1104	8	0.49476028117876325
1104	9	0.5565015653135745
1104	10	0.5981281383482155
1104	11	0.5430209845424656
1104	12	0.6160327636698639
1104	13	0.6846097398877049
1104	14	0.6666438334758666
1104	15	0.6836469180886269
1104	16	0.7385235262473344
1104	17	0.6205352123420482
1104	18	0.6224810969644352
1104	19	0.718558509471805
1104	20	0.7325870972964452
1104	21	0.6765575513185667
1104	22	0.7160716621613972
1104	23	0.6940220880079906
1104	24	0.6580058180499009
1104	25	0.6711428110020066
1105	1	0.37205594813337584
1105	2	0.4840956538767536
1105	3	0.40631535959357024
1105	4	0.37191043586808803
1105	5	0.46281047528400165
1105	6	0.5072619103175384
1105	7	0.4788992195280102
1105	8	0.4929457996251969
1105	9	0.5371085591625615
1105	10	0.5910095662360675
1105	11	0.542245542524924
1105	12	0.649552222275581
1105	13	0.5441163400193787
1105	14	0.6351670757493295
1105	15	0.6071939237143553
1105	16	0.6412884219656121
1105	17	0.704486105011803
1105	18	0.7163991658667612
1105	19	0.6520728112561827
1105	20	0.6724531638530209
1105	21	0.6702255743491747
1105	22	0.7132900967183389
1105	23	0.742853468345146
1105	24	0.7066489144452817
1105	25	0.8154769634304557
1106	1	0.393579632171285
1106	2	0.5094383872448809
1106	3	0.4363352947401751
1106	4	0.3724420397775461
1106	5	0.42301290685853843
1106	6	0.48553174493282825
1106	7	0.47469344546537684
1106	8	0.47064695495107145
1106	9	0.5128715277026785
1106	10	0.4877278247199115
1106	11	0.5374365805500076
1106	12	0.5377593994987464
1106	13	0.6471456837566336
1106	14	0.7005666200073506
1106	15	0.7602807342905393
1106	16	0.6168374940829295
1106	17	0.5092545692640087
1106	18	0.5792499203507475
1106	19	0.581439934520418
1106	20	0.718442320730361
1106	21	0.6818367277642423
1106	22	0.6631040068305012
1106	23	0.744202441784914
1106	24	0.7680802522675876
1106	25	0.7654243449104428
1107	1	0.2301405577651785
1107	2	0.385320262471911
1107	3	0.36849426294802967
1107	4	0.282472770439135
1107	5	0.37166502197091944
1107	6	0.3544209607731261
1107	7	0.3500614528267718
1107	8	0.39191445135391945
1107	9	0.3339968002429998
1107	10	0.2967869278367843
1107	11	0.42618875941791856
1107	12	0.39863054767174333
1107	13	0.42624434522311105
1107	14	0.3866693287443468
1107	15	0.42278774781385664
1107	16	0.5107900290508027
1107	17	0.4842725446645204
1107	18	0.5527936219782177
1107	19	0.5278417372972299
1107	20	0.4167631481723362
1107	21	0.49293534268170447
1107	22	0.4470226524536898
1107	23	0.5154285012529414
1107	24	0.5191020446872514
1107	25	0.42124076889881223
1108	1	0.16209898931324318
1108	2	0.32123480713014274
1108	3	0.2816175820926041
1108	4	0.2596874352656542
1108	5	0.25731675839233503
1108	6	0.3321783936729119
1108	7	0.3867516856735236
1108	8	0.2970982345191402
1108	9	0.30531871520544485
1108	10	0.3560758256218719
1108	11	0.35671937250648256
1108	12	0.3522127393098913
1108	13	0.3689138249020516
1108	14	0.4326459506267568
1108	15	0.3998194908035373
1108	16	0.32707535241681995
1108	17	0.48127859184839394
1108	18	0.4462000983429846
1108	19	0.4209877535289692
1108	20	0.5159668652126044
1108	21	0.4728326826201439
1108	22	0.43026570561406174
1108	23	0.43148269302517406
1108	24	0.468735030977804
1108	25	0.5438442371418191
1109	1	0.1730214302472497
1109	2	0.2782955390832136
1109	3	0.24212372279729877
1109	4	0.269642313122624
1109	5	0.2886819749361962
1109	6	0.19161995973958312
1109	7	0.2297405953780398
1109	8	0.26185347444778273
1109	9	0.28296781923835823
1109	10	0.3235801433587042
1109	11	0.34973753168416266
1109	12	0.3455838627209218
1109	13	0.34958119685054356
1109	14	0.37221346070300854
1109	15	0.47299305021912375
1109	16	0.42019909966563773
1109	17	0.33991549748028405
1109	18	0.40014903757927556
1109	19	0.3533233766713977
1109	20	0.3586543718460007
1109	21	0.4164470802732012
1109	22	0.3899398737831916
1109	23	0.3514067276075039
1109	24	0.3497143581679367
1109	25	0.3115625796475143
1110	1	-0.2255990862724886
1110	2	0.006025336111346036
1110	3	-0.10841717329377687
1110	4	-0.04604301844741343
1110	5	-0.12148243635907363
1110	6	-0.20456879785250237
1110	7	-0.09361113967420209
1110	8	-0.05832497078326071
1110	9	-0.12107562662077953
1110	10	-0.03917253106554597
1110	11	-0.06880104892311323
1110	12	-0.13275548763347444
1110	13	-0.14817947136825182
1110	14	-0.1488429339858257
1110	15	-0.18747784766761555
1110	16	-0.11100453960133194
1110	17	-0.12027790071280009
1110	18	-0.1689034559682696
1110	19	-0.14374190463541225
1110	20	-0.07752291429446956
1110	21	-0.22244924238863462
1110	22	-0.1049341945707809
1110	23	-0.18384404711044766
1110	24	-0.14865851256186602
1110	25	-0.12966159609959504
1111	1	-0.25665802923068526
1111	2	-0.12992316054190312
1111	3	-0.17475598051672847
1111	4	-0.14148813965496743
1111	5	-0.10922519224368443
1111	6	-0.3589292567122371
1111	7	-0.19708029792916193
1111	8	-0.1420903683397441
1111	9	-0.1941247911890045
1111	10	-0.29730749867417927
1111	11	-0.3395430909780828
1111	12	-0.18110031756983602
1111	13	-0.17272615367460276
1111	14	-0.18202471303582257
1111	15	-0.18801653795163986
1111	16	-0.22551590706935087
1111	17	-0.22154674186955958
1111	18	-0.24659627964876307
1111	19	-0.29253485099416565
1111	20	-0.3346126057335326
1111	21	-0.2451216734986943
1111	22	-0.3198883875128331
1111	23	-0.23858536457452573
1111	24	-0.2877466938387201
1111	25	-0.2337332050500862
1112	1	-0.4668731976929621
1112	2	-0.42460337936762116
1112	3	-0.42506168295506797
1112	4	-0.39531894423398484
1112	5	-0.3880259853260597
1112	6	-1.139021687042387
1112	7	-0.5882250015984383
1112	8	-0.43460840486826413
1112	9	-0.5077885303590435
1112	10	-0.5453169194903964
1112	11	-0.537930174097653
1112	12	-0.5244887298304448
1112	13	-0.4652645082826581
1112	14	-0.5561670784327788
1112	15	-0.6051222444535966
1112	16	-0.6001110270052834
1112	17	-0.6006882860774709
1112	18	-0.5981275009177267
1112	19	-0.6325421655027981
1112	20	-0.5966772273854986
1112	21	-0.5684199073496288
1112	22	-0.7089216194250909
1112	23	-0.6233590804295659
1112	24	-0.627099850844985
1112	25	-0.5523456664669055
1113	1	-0.7788804604301518
1113	2	-0.7668916530427552
1113	3	-0.6329396338987117
1113	4	-0.5669814826848452
1113	5	-0.694701273753175
1113	6	-1.2812336069915347
1113	7	-0.8641103760239588
1113	8	-0.7981186304222874
1113	9	-0.8618148194218307
1113	10	-0.8506543737329579
1113	11	-0.8858488957390023
1113	12	-0.9276247125284698
1113	13	-0.8658570716489464
1113	14	-1.0047733489368582
1113	15	-1.051197882135078
1113	16	-1.0414151048057079
1113	17	-1.0983176672834958
1113	18	-1.0346070461563954
1113	19	-1.085375290598698
1113	20	-1.1393695547493579
1113	21	-1.1062460546648296
1113	22	-1.1121171507228098
1113	23	-1.1039962869791822
1113	24	-1.141588427012394
1113	25	-1.1293800000705758
1114	1	-0.9878518594594468
1114	2	-0.9711757534737906
1114	3	-1.00505888830984
1114	4	-0.8229048046892231
1114	5	-1.0452712487990894
1114	6	-1.5222171339403232
1114	7	-1.175712455244323
1114	8	-1.4041314709729562
1114	9	-1.3911978259060547
1114	10	-1.5137685160245766
1114	11	-1.2116373713777477
1114	12	-1.3021845788431818
1114	13	-1.3240200459628468
1114	14	-1.5414751135883193
1114	15	-1.5825545532402003
1114	16	-1.6552440665284607
1114	17	-1.6315493217768733
1114	18	-1.7354440780375526
1114	19	-1.6309209960166342
1114	20	-1.5201534130736434
1114	21	-1.5453249818651358
1114	22	-1.5458280426888393
1114	23	-1.548862314915688
1114	24	-1.7695007473982614
1114	25	-1.6924523956843984
1115	1	-1.2095753854330447
1115	2	-1.787050697647576
1115	3	-1.355318933509885
1115	4	-0.9132258696721962
1115	5	-1.018876857365541
1115	6	-1.629047208489979
1115	7	-1.3219804690048917
1115	8	-1.3349987100225533
1115	9	-1.3298189529742004
1115	10	-1.5171850146194998
1115	11	-1.4554014320980073
1115	12	-1.3838437836067428
1115	13	-1.4062898951255107
1115	14	-1.4715779606186459
1115	15	-1.4253995253600849
1115	16	-1.571416406286943
1115	17	-1.472814632793039
1115	18	-1.5723672149531112
1115	19	-1.5352834372114543
1115	20	-1.4856013467570648
1115	21	-1.650201034209985
1115	22	-1.7769505881146657
1115	23	-1.5950803008912402
1115	24	-1.5951328042236383
1115	25	-1.601421364382766
1116	1	-1.0466541637432556
1116	2	-1.0728589822694587
1116	3	-1.1860364951057498
1116	4	-0.952014564733993
1116	5	-0.9535842960256389
1116	6	-1.1586266465498412
1116	7	-1.2301592864493003
1116	8	-1.2314415095370563
1116	9	-1.320229929252399
1116	10	-1.4062414068257283
1116	11	-1.4125424217056732
1116	12	-1.2522598679593457
1116	13	-1.2260280306722393
1116	14	-1.5544375119522087
1116	15	-1.4601056422287542
1116	16	-1.5151135928882837
1116	17	-1.5217391377210276
1116	18	-1.4513164870433428
1116	19	-1.4916458687514478
1116	20	-1.4478007862869404
1116	21	-1.5172021523079948
1116	22	-1.5030298308091687
1116	23	-1.6288597502894717
1116	24	-1.6814368446725965
1116	25	-1.825249817860822
1201	1	0.7425346964044479
1201	2	0.9375259469807027
1201	3	0.8520561475018624
1201	4	0.7696496890994524
1201	5	0.8594590116987249
1201	6	0.8724137820752915
1201	7	0.8391494750388233
1201	8	0.846901622853629
1201	9	1.0101759463106106
1201	10	1.0562133461840304
1201	11	1.044231917431027
1201	12	1.0549368103792531
1201	13	1.0035273593866165
1201	14	1.2863153429507648
1201	15	1.2336280889758484
1201	16	1.1784801566341585
1201	17	1.2536534782961724
1201	18	1.2287929717043369
1201	19	1.2435131184494992
1201	20	1.1678297884020523
1201	21	1.2267855121883073
1201	22	1.215669490778879
1201	23	1.2369601154951937
1201	24	1.2501537812411314
1201	25	1.3405218591788142
1202	1	0.5000941549415252
1202	2	0.7185432040832787
1202	3	0.6942310767278812
1202	4	0.6693057499259183
1202	5	0.6857185368136953
1202	6	0.7406875263266528
1202	7	0.6819427674556593
1202	8	0.8297745989415333
1202	9	0.8748400076741731
1202	10	0.9483117667773016
1202	11	0.9038420031430761
1202	12	0.9457101769828853
1202	13	0.9389675008226845
1202	14	1.08549822515824
1202	15	1.1694339892320897
1202	16	1.090664401170855
1202	17	1.1381841181153547
1202	18	1.1526270892395019
1202	19	1.1652404511935117
1202	20	1.1715006642090984
1202	21	1.2114518953860625
1202	22	1.1989287165730573
1202	23	1.2342836421095669
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2211	6	-0.12286150733115875
2211	7	-0.2274253277232816
2211	8	-0.23111361061895508
2211	9	-0.2701016701585246
2211	10	-0.25412288976747277
2211	11	-0.25686468449502087
2211	12	-0.21979100765147308
2211	13	-0.26926681719174184
2211	14	-0.29890253620227014
2211	15	-0.26916368320542006
2211	16	-0.3633036247934882
2211	17	-0.3623859594404877
2211	18	-0.29509731467200595
2211	19	-0.3172052197887112
2211	20	-0.21998427718034463
2211	21	-0.2755619386586403
2211	22	-0.19262638223521122
2211	23	-0.298100634198633
2211	24	-0.3107341302658071
2211	25	-0.31155372606690435
2212	1	-0.36994503629965436
2212	2	-0.29827354828502095
2212	3	-0.38611991890651254
2212	4	-0.4667469565776301
2212	5	-0.3846158289654653
2212	6	-0.27764295268219286
2212	7	-0.5629391842983509
2212	8	-0.5246104199530919
2212	9	-0.46757992744566934
2212	10	-0.5143630516100117
2212	11	-0.5228493458811343
2212	12	-0.5322258228790702
2212	13	-0.5552925029734936
2212	14	-0.6224267312719647
2212	15	-0.5897767073668803
2212	16	-0.6824732060280851
2212	17	-0.5577240962997791
2212	18	-0.6437506867168779
2212	19	-0.6503092183663028
2212	20	-0.588602565994691
2212	21	-0.5897706470189874
2212	22	-0.635258361749031
2212	23	-0.6326888399017179
2212	24	-0.6813713310663445
2212	25	-0.8099944954694678
2213	1	-0.606361654747559
2213	2	-0.9553130347499952
2213	3	-0.7416501755695784
2213	4	-0.5590524957121161
2213	5	-0.6769364081754858
2213	6	-0.5930644612435501
2213	7	-0.6711560788987541
2213	8	-0.7105519182370585
2213	9	-0.7697881029800584
2213	10	-0.7609712012420502
2213	11	-0.6840183775300517
2213	12	-0.7973583322112248
2213	13	-0.8262542949149392
2213	14	-0.8676547450837688
2213	15	-0.9406144604631521
2213	16	-0.8932746958311171
2213	17	-0.9442908021681262
2213	18	-1.032755347051392
2213	19	-0.9008516010902667
2213	20	-0.9630622199476164
2213	21	-1.017832921287301
2213	22	-0.9425396383172213
2213	23	-1.0368444963279018
2213	24	-1.0789954169117593
2213	25	-0.9931153529208885
2214	1	-0.8077097662100208
2214	2	-0.829583171243506
2214	3	-0.9144238578486892
2214	4	-0.792067795918868
2214	5	-0.8999851758804311
2214	6	-0.7923035810752097
2214	7	-0.799279016969848
2214	8	-0.7666623975366278
2214	9	-0.9291244160941403
2214	10	-0.9653290245600772
2214	11	-1.0747755233791523
2214	12	-1.0267930887665315
2214	13	-0.957541609074994
2214	14	-1.2118445058591192
2214	15	-1.1980505120133174
2214	16	-1.119637818287508
2214	17	-1.0909466273930946
2214	18	-1.1774456560587367
2214	19	-1.1367779738060264
2214	20	-1.1532744662123378
2214	21	-1.364343938134958
2214	22	-1.2797513721986036
2214	23	-1.3352851100488294
2214	24	-1.4252172613261607
2214	25	-1.3055342364987865
2215	1	-1.3886327252577928
2215	2	-3.074986682745827
2215	3	-1.716372413411893
2215	4	-1.4884402278621849
2215	5	-1.3846352278077276
2215	6	-1.2885023833055413
2215	7	-1.4477098548998129
2215	8	-1.5238276560308108
2215	9	-1.6626534469941276
2215	10	-1.6664939318840766
2215	11	-1.7595676396084983
2215	12	-1.777913191962682
2215	13	-1.851402415988261
2215	14	-2.095731241502797
2215	15	-1.9669005194457512
2215	16	-2.095379420880576
2215	17	-2.0795215847688198
2215	18	-2.0839989506236805
2215	19	-2.0519112738180993
2215	20	-2.0534122406513404
2215	21	-2.013655574737669
2215	22	-2.092947629442733
2215	23	-2.1707776411977235
2215	24	-2.2776364348192004
2215	25	-2.2107498120936597
2216	1	-1.1888296766620883
2216	2	-1.8980028689707247
2216	3	-1.3601778668961053
2216	4	-1.1906417922090284
2216	5	-1.3189957252727567
2216	6	-1.205469885154514
2216	7	-1.466592439239001
2216	8	-1.4855610466605143
2216	9	-1.6370727999154155
2216	10	-1.638283888064045
2216	11	-1.6450342591128475
2216	12	-1.6684430177446856
2216	13	-1.8414798148109597
2216	14	-1.8919023627674463
2216	15	-1.9410510861244035
2216	16	-1.9099768602784621
2216	17	-1.8454448992274806
2216	18	-2.096877693767361
2216	19	-1.9893536144723072
2216	20	-2.0204757918765353
2216	21	-1.9797897554336572
2216	22	-1.9464362866126237
2216	23	-2.0496193970001997
2216	24	-1.892917766823409
2216	25	-2.1090968897351132
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40444.0
3	0.0
4	711.0
5	6165.0
6	7776.0
7	6370.0
8	5265.0
9	4329.0
10	3801.0
11	3527.0
12	3191.0
13	2944.0
14	2960.0
15	2912.0
16	3074.0
17	3241.0
18	3708.0
19	4550.0
20	6508.0
21	8748.0
22	10897.0
23	12939.0
24	14936.0
25	17456.0
26	19675.0
27	22053.0
28	25240.0
29	30567.0
30	39422.0
31	51865.0
32	67274.0
33	89369.0
34	121825.0
35	167212.0
36	245053.0
37	431263.0
38	1359721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72078803246366	19.73013687718133	13.523292101699527	25.025782988655486
2	14.533244528565417	22.393695985588895	40.30450761479808	22.768551871047606
3	17.6961922254057	27.093130958264354	30.46725472613015	24.743422090199793
4	11.73906064332483	18.676139123727474	39.2183185686221	30.3664816643256
5	13.234885837688148	37.639368689619786	34.87789535092128	14.247850121770789
6	28.921862206393595	39.83468223645456	18.054073914575284	13.189381642576562
7	25.66559964648883	32.739160108877506	23.510916729923295	18.08432351471037
8	24.2255494055819	36.24819370379763	21.188583294754523	18.337673595865944
9	25.938769767849507	15.956021134416986	20.557228624692677	37.54798047304083
10	14.429065257714575	29.21568489685796	33.76204963183632	22.593200213591143
11	33.4092071467512	23.61326555272668	24.980153377508753	17.99737392301337
12	23.01139896373057	25.591112672345655	31.79794502502854	19.59954333889523
13	28.26802712676112	22.664244500591607	26.414128568256746	22.653599804390534
14	21.56060769449902	22.2127928326023	28.261285732400715	27.965313740497965
15	22.53804706210527	30.02070327911414	24.838103027358333	22.60314663142226
16	21.831501213860385	28.9673351944337	27.005662339906905	22.19550125179901
17	21.983942178635445	27.57548113924331	27.62086484052301	22.819711841598238
18	22.593324100180297	26.802791863819543	29.46943558224888	21.134448453751283
19	24.111001126155397	26.332927505239116	27.9963777559369	21.559693612668582
20	23.00793503040181	27.169066378162682	28.50383052264417	21.31916806879134
21	22.995231697357678	26.669020083072088	28.013277483600728	22.32247073596951
22	22.34861297418924	27.24328949406584	28.556957152305717	21.851140379439205
23	22.147080270946585	26.612029612477816	28.41092862853313	22.829961488042468
24	22.093528091024304	27.083222100764065	28.87528416994276	21.947965638268872
25	22.35090678475423	26.78006847383161	28.811522037621618	22.057502703792544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	745.0
1	745.0
2	1446.0
3	2147.0
4	2147.0
5	2147.0
6	5984.5
7	9822.0
8	9822.0
9	9822.0
10	11554.5
11	13287.0
12	13287.0
13	13287.0
14	17032.5
15	20778.0
16	20778.0
17	20778.0
18	34973.0
19	49168.0
20	49168.0
21	49168.0
22	72262.0
23	95356.0
24	95356.0
25	95356.0
26	131479.0
27	167602.0
28	167602.0
29	167602.0
30	202718.0
31	237834.0
32	237834.0
33	237834.0
34	254359.5
35	270885.0
36	270885.0
37	270885.0
38	284690.5
39	298496.0
40	298496.0
41	298496.0
42	313133.0
43	327770.0
44	327770.0
45	327770.0
46	328950.0
47	330130.0
48	330130.0
49	330130.0
50	319416.5
51	308703.0
52	308703.0
53	308703.0
54	275243.0
55	241783.0
56	241783.0
57	241783.0
58	212374.0
59	182965.0
60	182965.0
61	182965.0
62	152830.5
63	122696.0
64	122696.0
65	122696.0
66	99007.5
67	75319.0
68	75319.0
69	75319.0
70	57429.0
71	39539.0
72	39539.0
73	39539.0
74	31400.0
75	23261.0
76	23261.0
77	23261.0
78	18827.5
79	14394.0
80	14394.0
81	14394.0
82	11112.5
83	7831.0
84	7831.0
85	7831.0
86	6098.5
87	4366.0
88	4366.0
89	4366.0
90	3147.0
91	1928.0
92	1928.0
93	1928.0
94	1045.5
95	163.0
96	163.0
97	163.0
98	93.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34594419160439915
2	0.010432066697787243
3	0.0
4	0.0
5	0.052019834274151196
6	0.003863728406587868
7	0.004285226050942908
8	1.756240184812667E-4
9	0.00913244896102587
10	0.015138790393085192
11	0.005584843787704282
12	0.008465077690797056
13	0.01766777625921543
14	0.0035124803696253344
15	0.020442635751219445
16	0.010115943464520964
17	0.005409219769223015
18	1.4049921478501335E-4
19	0.004812098106386708
20	0.004250101247246655
21	0.0023182370439527207
22	0.0
23	0.0029153587067890273
24	0.0036178547807140943
25	0.0022479874365602137
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2846991.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.52734227373534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.27155589228116	60.957982523207
2	5.9758394013264375	8.070651052524882
3	1.3509897823845964	2.7368624833021165
4	0.6486998416104116	1.752199049493767
5	0.3870952786479646	1.306975768690403
6	0.24282226968771098	0.9838285510132396
7	0.1734003466051676	0.8196485188914046
8	0.12896565360485296	0.6966966266024717
9	0.100185565871644	0.6088738497752125
>10	0.6086411569573743	7.779594890181153
>50	0.05791563333911178	2.723686107588131
>100	0.04810773624695001	6.500251816619734
>500	0.004218889697150743	1.9688740966039873
>1k	0.0013021264496638676	1.4150963825611056
>5k	1.562551739596641E-4	0.6554941413180724
>10k+	1.041701159731094E-4	1.0232841416273004
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17331	0.6087479728597667	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11763	0.413173065879028	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6829	0.2398672844417141	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6415	0.2253256157114652	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5393	0.18942806633389428	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4721	0.16582419825001202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.024960739250669E-5	0.0
6	0.0	0.0	0.0	1.0537441108876002E-4	0.0
7	0.0	0.0	0.0	1.0537441108876002E-4	0.0
8	0.0	0.0	0.0	1.0537441108876002E-4	0.0
9	0.0	0.0	0.0	1.0537441108876002E-4	0.0
10	0.0	0.0	0.0	1.756240184812667E-4	0.0
11	0.0	0.0	0.0	2.1074882217752004E-4	0.0
12	0.0	0.0	0.0	2.1074882217752004E-4	0.0
13	0.0	0.0	0.0	2.1074882217752004E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1795	0.0	16.406784	1
GTATCAA	6100	0.0	13.593113	1
TGCGCGA	60	4.0993802E-4	12.664652	11
ATAGGCG	55	0.0030683323	12.091536	5
AACCGTG	55	0.0030768216	12.087076	7
AATTCCG	170	1.8189894E-12	11.735902	5
CGCGAGT	65	8.037733E-4	11.690449	13
CGGTCCA	170	1.2732926E-11	11.174497	10
ACACCGT	95	1.3667755E-5	10.995933	6
AACCGCG	130	3.285095E-8	10.958063	7
AAGACCG	70	0.001491418	10.857705	5
CGACCAT	150	2.1409505E-9	10.764766	10
CGAATAC	100	2.398925E-5	10.4505415	4
CGAGCCG	140	9.7203156E-8	10.176953	15
CCGATAA	160	6.197297E-9	10.091968	9
CGCGTAT	85	6.615794E-4	10.055634	7
TCGATTA	125	2.5770369E-6	9.878428	13
TCGCGTA	135	6.463488E-7	9.850112	9
ACGAACG	165	1.0257281E-8	9.786323	15
CGATAAC	165	1.0260919E-8	9.78615	10
>>END_MODULE