FastQCFastQC Report
Thu 2 Feb 2017
SRR4050258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803752
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102260.36472555347263236No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN96470.3440746542490206No Hit
TATCAACGCAGAGTACTTTTTTTTT62040.2212749201783895No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN43470.15504224339385222No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39750.14177430814137626No Hit
GGTATCAACGCAGAGTACTTTTTTT36170.12900570378549886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.015.6350871
GTATCAA52550.013.168311
AGCCGTC655.458512E-513.1530757
TTTTCGG1750.011.942800516
GGGTATA2750.011.4065071
TATCACG752.0750292E-411.3999462
ACCGAGC855.3283395E-511.1760178
ACTGTTC10350.011.1058288
AACCGCG951.36204E-510.9993557
TTGCGCA700.001492774610.8565064
TCGAACG1408.36917E-910.8565063
CGAACGT1158.8308116E-710.7385014
TATAACG803.7728585E-410.6874492
CGAACGA2150.010.60460116
GTCTAAA3500.010.5917561
GCGTTAT1451.4320904E-810.4887411
TAGGACC9400.010.3079064
TGGCGAG15050.010.09961918
ACGAACG2450.010.08122315
TACGCTC856.5967184E-410.05877612
Produced by FastQC (version 0.11.5)