##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050258_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2803752
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.950320142437704	31.0	31.0	34.0	26.0	34.0
2	29.99313705349118	31.0	31.0	34.0	26.0	34.0
3	30.13912072109088	31.0	31.0	34.0	26.0	34.0
4	33.553337813044806	37.0	35.0	37.0	28.0	37.0
5	33.62370673297781	37.0	35.0	37.0	28.0	37.0
6	33.67202127720283	37.0	35.0	37.0	29.0	37.0
7	33.560793536660874	37.0	35.0	37.0	28.0	37.0
8	33.5347117006069	37.0	35.0	37.0	28.0	37.0
9	34.98967383705834	39.0	35.0	39.0	28.0	39.0
10	34.88010922506698	39.0	35.0	39.0	27.0	39.0
11	34.90236725644779	39.0	35.0	39.0	27.0	39.0
12	34.83169766798205	39.0	35.0	39.0	27.0	39.0
13	34.82893850811342	39.0	35.0	39.0	27.0	39.0
14	35.89742102725205	40.0	36.0	41.0	27.0	41.0
15	35.9052351991189	40.0	36.0	41.0	27.0	41.0
16	35.91264419962964	40.0	36.0	41.0	27.0	41.0
17	35.902372963086606	40.0	36.0	41.0	27.0	41.0
18	35.84664513837173	40.0	36.0	41.0	27.0	41.0
19	35.9023155400335	40.0	37.0	41.0	27.0	41.0
20	35.878685418681826	40.0	37.0	41.0	27.0	41.0
21	35.80400655978132	40.0	36.0	41.0	27.0	41.0
22	35.723325030173854	40.0	36.0	41.0	27.0	41.0
23	35.668681110169516	40.0	36.0	41.0	27.0	41.0
24	35.618485158459094	40.0	36.0	41.0	27.0	41.0
25	35.29872934553413	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.2794901777526775
1101	2	1.3653899209180445
1101	3	1.3277421663306548
1101	4	1.3931672380094327
1101	5	1.3740361066089548
1101	6	1.3418919710979864
1101	7	1.3595975935469582
1101	8	1.3825387666984668
1101	9	1.4754335461724253
1101	10	1.573259610382948
1101	11	1.5149292683774576
1101	12	1.5256582980100006
1101	13	1.5072614846717656
1101	14	1.7815500516776552
1101	15	1.685791926792767
1101	16	1.7130188180961454
1101	17	1.697011387556934
1101	18	1.7405054798893644
1101	19	1.755948791139879
1101	20	1.7185786805449013
1101	21	1.7211156206077902
1101	22	1.7579277791546346
1101	23	1.8312330176361797
1101	24	1.8875245373386207
1101	25	1.8238778756189902
1102	1	1.1770356981558017
1102	2	1.220911139336227
1102	3	1.2318762406708146
1102	4	1.3395171084843014
1102	5	1.2900299334773564
1102	6	1.2462375331766395
1102	7	1.2482030918282874
1102	8	1.2672917225839484
1102	9	1.4529703049406706
1102	10	1.433841428257793
1102	11	1.4226275331338911
1102	12	1.484805398173215
1102	13	1.4908338635733784
1102	14	1.6812137817304418
1102	15	1.6484674510635386
1102	16	1.6744007571765707
1102	17	1.6678218587769393
1102	18	1.650893326630957
1102	19	1.7664307632541494
1102	20	1.6935271581775169
1102	21	1.7812285666119934
1102	22	1.790578198142505
1102	23	1.7298055420466838
1102	24	1.6813137733680463
1102	25	1.6626515755192202
1103	1	1.0726638686781271
1103	2	1.1256843267697612
1103	3	1.1016786568991108
1103	4	1.1967789155015325
1103	5	1.158555902117051
1103	6	1.1391003248980667
1103	7	1.072564929516517
1103	8	1.0846044301094437
1103	9	1.271961239605119
1103	10	1.305627235771098
1103	11	1.2542736804453298
1103	12	1.2525936818515007
1103	13	1.2632634810079963
1103	14	1.4655563244441083
1103	15	1.4978005412773783
1103	16	1.50992929789399
1103	17	1.4930595590211482
1103	18	1.448183528037184
1103	19	1.5435778122508097
1103	20	1.518269484894276
1103	21	1.480319995350051
1103	22	1.5180193268633815
1103	23	1.486434079004887
1103	24	1.492838761449221
1103	25	1.5162981395065458
1104	1	0.779972464678778
1104	2	0.849168993446856
1104	3	0.8831741539119307
1104	4	0.9298499837132042
1104	5	0.9106583779029052
1104	6	0.8862404445716479
1104	7	0.9002287850268331
1104	8	0.9394717403563888
1104	9	1.032859926471012
1104	10	1.0556090454137532
1104	11	1.0256169276621065
1104	12	1.1131961637951164
1104	13	1.0614423815914478
1104	14	1.2167587020719353
1104	15	1.1359447720050042
1104	16	1.2234341320688316
1104	17	1.2204008477372383
1104	18	1.2687403293835047
1104	19	1.2148523255464099
1104	20	1.2596794256499635
1104	21	1.3103349836719431
1104	22	1.3116397538524822
1104	23	1.3075808936619566
1104	24	1.3349510530381536
1104	25	1.3807935909186568
1105	1	0.5722292213033349
1105	2	0.7250957720004862
1105	3	0.7670913946296309
1105	4	0.7921549249648194
1105	5	0.7693278081352304
1105	6	0.7152016491463016
1105	7	0.6807818116266731
1105	8	0.7005898483910613
1105	9	0.8349219343962773
1105	10	0.8711629382576263
1105	11	0.7678550813410538
1105	12	0.8509650031827434
1105	13	0.8182938354140177
1105	14	0.9397657964369941
1105	15	0.9099935524312315
1105	16	1.0528810644446764
1105	17	0.9858423497570499
1105	18	0.8687310515785711
1105	19	0.8961246976002784
1105	20	0.902096476866916
1105	21	0.9860796774144376
1105	22	0.9456124247210909
1105	23	0.9185928276330131
1105	24	0.8147366410726846
1105	25	0.8305796175977136
1106	1	0.5350552910195105
1106	2	0.5864446706962347
1106	3	0.587267631931045
1106	4	0.7248507725120419
1106	5	0.6811973510309102
1106	6	0.6513112745197986
1106	7	0.6042303288804121
1106	8	0.6138840298052912
1106	9	0.6685612203597273
1106	10	0.7212499657110811
1106	11	0.7015261491700002
1106	12	0.6535701024727487
1106	13	0.6478515069779505
1106	14	0.7084997750674162
1106	15	0.8013814420497738
1106	16	0.82386195537444
1106	17	0.855362050041542
1106	18	0.8159891948617144
1106	19	0.7950011221940727
1106	20	0.7786437260063295
1106	21	0.8406422235890787
1106	22	0.9101930561486427
1106	23	0.9183736937574238
1106	24	0.9030320895875548
1106	25	0.9536462754393966
1107	1	0.5930171528563548
1107	2	0.7046343027852551
1107	3	0.6166632422606249
1107	4	0.6584820098886581
1107	5	0.697291849251684
1107	6	0.615179480865919
1107	7	0.5069097510103404
1107	8	0.49801036251360387
1107	9	0.6049244733525683
1107	10	0.6411158694636256
1107	11	0.612880410870936
1107	12	0.6467848913222483
1107	13	0.6658850042973654
1107	14	0.7209848995792356
1107	15	0.6404318313409973
1107	16	0.6945352925682897
1107	17	0.7043503454194493
1107	18	0.684408623457827
1107	19	0.7586567603426602
1107	20	0.7846427776778881
1107	21	0.7591728904943693
1107	22	0.7457480699981431
1107	23	0.7528048389754431
1107	24	0.8922811101452979
1107	25	0.7871340635678692
1108	1	0.4614442083755783
1108	2	0.5802359511379613
1108	3	0.5420101387444092
1108	4	0.5935391091040714
1108	5	0.603059483049698
1108	6	0.4563620276717302
1108	7	0.46759627383971036
1108	8	0.5117230741772403
1108	9	0.45731960077030465
1108	10	0.5811072236262902
1108	11	0.5720997596366288
1108	12	0.528663050142832
1108	13	0.571384429017499
1108	14	0.677222485077067
1108	15	0.7684047455244922
1108	16	0.6213329163326691
1108	17	0.7026503861183642
1108	18	0.6033906148904293
1108	19	0.719781130641266
1108	20	0.7479706868731668
1108	21	0.6907931071001556
1108	22	0.7525963124503505
1108	23	0.6098574863382069
1108	24	0.6705851849815687
1108	25	0.7660275603063198
1109	1	0.35769757204202435
1109	2	0.4880428528236216
1109	3	0.3995126635843427
1109	4	0.5475317640572683
1109	5	0.527014000790679
1109	6	0.3399721352697753
1109	7	0.36528152139611336
1109	8	0.293565703582118
1109	9	0.3665089671095316
1109	10	0.4414264856946417
1109	11	0.4642769652341343
1109	12	0.42729055426756446
1109	13	0.35442739912316057
1109	14	0.3536500082900389
1109	15	0.37988175007966163
1109	16	0.5057871851726787
1109	17	0.4315033322002222
1109	18	0.336880635336513
1109	19	0.44961359677581925
1109	20	0.42075137831569975
1109	21	0.5111951620486082
1109	22	0.5895044985159501
1109	23	0.5135284597506597
1109	24	0.4366043694123576
1109	25	0.5312095903041651
1110	1	0.1201637744082511
1110	2	0.34478877436542277
1110	3	0.21323014482056735
1110	4	0.1695437767191308
1110	5	0.1791020503641434
1110	6	0.25678266855005205
1110	7	0.15850352040467186
1110	8	0.20186255931516683
1110	9	0.28366704445847546
1110	10	0.2916039345428487
1110	11	0.3282787333107322
1110	12	0.2586601483994073
1110	13	0.3108369172421348
1110	14	0.3438619690958049
1110	15	0.32018672325595077
1110	16	0.3638177874848836
1110	17	0.36133128937044745
1110	18	0.356164872885536
1110	19	0.3493768380144786
1110	20	0.423778387969989
1110	21	0.3747856679741943
1110	22	0.4847033810851329
1110	23	0.41929672950817576
1110	24	0.4074615619615898
1110	25	0.4621140424348553
1111	1	-0.17527023772347405
1111	2	-0.13867306174607208
1111	3	-0.21027799510758527
1111	4	-0.23410435070584867
1111	5	-0.1959940755479792
1111	6	-0.25996051700305856
1111	7	-0.3618025914474856
1111	8	-0.23306270988903321
1111	9	-0.23462049366090554
1111	10	-0.21742063369101317
1111	11	-0.29017580802945986
1111	12	-0.19109423874496656
1111	13	-0.19589621581753391
1111	14	-0.2758911992799824
1111	15	-0.22887321931357008
1111	16	-0.24808023895507603
1111	17	-0.2533474167517653
1111	18	-0.27970176829152393
1111	19	-0.3437471851162073
1111	20	-0.3221840420329798
1111	21	-0.2630719415454976
1111	22	-0.21703131721893953
1111	23	-0.35335048844126504
1111	24	-0.3459441826262051
1111	25	-0.40618754250890277
1112	1	-0.3941460287294518
1112	2	-0.31259471503292247
1112	3	-0.4162922808755738
1112	4	-0.3717365713904073
1112	5	-0.36357237598803493
1112	6	-0.3991785535235408
1112	7	-0.49652233667155343
1112	8	-0.4668426905169838
1112	9	-0.5832574241773258
1112	10	-0.6177028190487022
1112	11	-0.48817446512502727
1112	12	-0.570674179239731
1112	13	-0.5399467392405839
1112	14	-0.7619201241741749
1112	15	-0.7039303178129828
1112	16	-0.5966559941061078
1112	17	-0.5759492842517417
1112	18	-0.6178114790372149
1112	19	-0.48157067391462505
1112	20	-0.4452166968791502
1112	21	-0.4701410660387566
1112	22	-0.5195326691631905
1112	23	-0.6621722804400108
1112	24	-0.5512811537233304
1112	25	-0.6153803069354993
1113	1	-0.6347875939538561
1113	2	-0.6052555822283985
1113	3	-0.6213651340531108
1113	4	-0.5828775882575599
1113	5	-0.5816135336571975
1113	6	-0.7326075750694656
1113	7	-0.6582130886992488
1113	8	-0.7458895491622712
1113	9	-0.7820250620665945
1113	10	-0.8160152362146036
1113	11	-0.7317402621261806
1113	12	-0.7293522835822372
1113	13	-0.7699416511793586
1113	14	-0.9583833347357071
1113	15	-1.0054515307414533
1113	16	-0.987145172662359
1113	17	-0.9993901660326543
1113	18	-0.9545455714460047
1113	19	-1.0521944436831703
1113	20	-1.1088976361444338
1113	21	-0.9965797191774044
1113	22	-1.0077151749083129
1113	23	-1.1363268942194154
1113	24	-1.2409049536017633
1113	25	-1.230114684112408
1114	1	-0.8118948849326273
1114	2	-0.8153949693153031
1114	3	-0.9071075483798268
1114	4	-0.8415933446038295
1114	5	-0.8687795457221981
1114	6	-0.9349791571395514
1114	7	-0.8889739546413864
1114	8	-0.8594173538094623
1114	9	-1.2002325673296426
1114	10	-1.0206310607767364
1114	11	-1.0110270449809349
1114	12	-1.233736481131686
1114	13	-1.132687877607843
1114	14	-1.2105635114104984
1114	15	-1.2416654579323207
1114	16	-1.1310580784634112
1114	17	-1.5573530232047474
1114	18	-1.3351919439759001
1114	19	-1.310829633035823
1114	20	-1.2968441786034504
1114	21	-1.3352541120248702
1114	22	-1.317566550462871
1114	23	-1.5307598758466767
1114	24	-1.4068682576257103
1114	25	-1.5843195716288392
1115	1	-1.249061315885772
1115	2	-1.2564022833166888
1115	3	-1.3464286289112692
1115	4	-1.3971958206281485
1115	5	-1.3002840130956628
1115	6	-1.4730724842101282
1115	7	-1.4753231736897874
1115	8	-1.5255724834174913
1115	9	-2.123464264260548
1115	10	-1.8292569498640603
1115	11	-1.836073305910645
1115	12	-1.7690606044322266
1115	13	-1.655373476199756
1115	14	-1.9635507932034386
1115	15	-1.8803127547971599
1115	16	-2.0872885933856224
1115	17	-1.986649934497315
1115	18	-1.7829561280802082
1115	19	-1.9069510702345127
1115	20	-1.9225168009078004
1115	21	-1.921362671006591
1115	22	-2.025598789878991
1115	23	-1.8858260605910218
1115	24	-1.9772984262038946
1115	25	-1.8354502868215192
1116	1	-1.5235983607922954
1116	2	-1.605469725662811
1116	3	-1.53018882169388
1116	4	-1.7386307416234636
1116	5	-1.3887521604982354
1116	6	-1.5626003562193773
1116	7	-1.6034051396887534
1116	8	-1.4892752651547454
1116	9	-1.9207685039481746
1116	10	-2.6627139999428593
1116	11	-2.150020495883716
1116	12	-1.9448653121743646
1116	13	-1.9259664332844366
1116	14	-2.1401603301026455
1116	15	-2.883597939020021
1116	16	-2.3106573687461704
1116	17	-2.2031268195246696
1116	18	-2.2634789741187973
1116	19	-2.201823141403082
1116	20	-2.270208232778245
1116	21	-2.3685845154935024
1116	22	-2.2749880728267513
1116	23	-2.252366551860078
1116	24	-2.199504122506326
1116	25	-2.4218343471064614
1201	1	1.1437385976482553
1201	2	1.170556175383556
1201	3	1.0730018530577752
1201	4	1.1227632808783525
1201	5	1.105555754863964
1201	6	1.080556439358503
1201	7	1.0474239194606696
1201	8	1.0008404979433152
1201	9	1.186068335949841
1201	10	1.296810036324139
1201	11	1.2742037943461284
1201	12	1.2769636016648533
1201	13	1.2343843206157032
1201	14	1.5089476883910393
1201	15	1.5033242158831754
1201	16	1.4123867763264286
1201	17	1.372746479615195
1201	18	1.434652223500251
1201	19	1.4281114731953863
1201	20	1.466036773427561
1201	21	1.5369991050815415
1201	22	1.5749104667061644
1201	23	1.5413182058747026
1201	24	1.64240225099622
1201	25	1.6853782878201429
1202	1	0.9091173791340381
1202	2	0.9462936476059589
1202	3	0.8781880143506804
1202	4	0.9452073632493381
1202	5	0.9317726137829823
1202	6	0.9558191770233009
1202	7	0.9361620646203903
1202	8	0.8615429060202899
1202	9	0.9500772247810261
1202	10	1.06180171656856
1202	11	0.9905492470425301
1202	12	1.0059241407071156
1202	13	1.1182098069806088
1202	14	1.2884854172384124
1202	15	1.1716937818061481
1202	16	1.1962235143763849
1202	17	1.1663970671911699
1202	18	1.225857733363867
1202	19	1.1455509358841454
1202	20	1.1443543435828971
1202	21	1.1649969759165302
1202	22	1.2273552198506863
1202	23	1.200473507257584
1202	24	1.1578094862624653
1202	25	1.2756198312051268
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2211	10	-0.5191085553739612
2211	11	-0.4477755686041718
2211	12	-0.5130679695504483
2211	13	-0.49003872861197095
2211	14	-0.6442878774971348
2211	15	-0.39362561318816347
2211	16	-0.5003291955847331
2211	17	-0.5336259050068577
2211	18	-0.551530996105086
2211	19	-0.6311771603497007
2211	20	-0.5169559816056974
2211	21	-0.520313174031827
2211	22	-0.4474799447993192
2211	23	-0.513153387176466
2211	24	-0.4344390538234606
2211	25	-0.46037732226432126
2212	1	-0.697172112457288
2212	2	-0.663495900828547
2212	3	-0.6363386302707177
2212	4	-0.691965108835717
2212	5	-0.7265761660658612
2212	6	-0.6311670306800252
2212	7	-0.6370395902574941
2212	8	-0.6705326278524737
2212	9	-0.82415156159243
2212	10	-0.8690987287853744
2212	11	-0.89287973111486
2212	12	-0.7741772872923463
2212	13	-0.7574199577545428
2212	14	-0.9083101104392739
2212	15	-0.8771765218352741
2212	16	-1.0210121607310825
2212	17	-1.0416934469182948
2212	18	-0.991248602920372
2212	19	-0.9925562357476494
2212	20	-1.0022063146775722
2212	21	-0.9637027322884961
2212	22	-0.8216655122564873
2212	23	-0.927424629769078
2212	24	-0.8563749459565813
2212	25	-1.0574266500374492
2213	1	-0.8439617788547977
2213	2	-0.7621856297941143
2213	3	-0.7401985088065679
2213	4	-0.7599732057570705
2213	5	-0.8159276402627995
2213	6	-0.8976063383290338
2213	7	-0.8885566009968358
2213	8	-0.9061422110858501
2213	9	-0.8619893561600307
2213	10	-1.047449199259475
2213	11	-0.9792069964052388
2213	12	-1.0866013205538678
2213	13	-1.1568503035125701
2213	14	-1.1487469196697049
2213	15	-1.0998847246032994
2213	16	-1.2784545017560092
2213	17	-1.218196184238579
2213	18	-1.1456796377103302
2213	19	-1.1525108448547954
2213	20	-1.2535911569563964
2213	21	-1.17686648716046
2213	22	-1.204342956914651
2213	23	-1.163030813032286
2213	24	-1.2833027441040485
2213	25	-1.2022462273927559
2214	1	-1.3083522429164063
2214	2	-1.3484796856420012
2214	3	-1.281852196113963
2214	4	-1.2668878975610909
2214	5	-1.3002767699606963
2214	6	-1.3092064694202108
2214	7	-1.4173127843635953
2214	8	-1.3948343424121887
2214	9	-1.486568082744192
2214	10	-1.9295738430884342
2214	11	-1.7950496866544867
2214	12	-1.7618395630523125
2214	13	-1.6919674530838762
2214	14	-1.916619486933584
2214	15	-1.8247856682239743
2214	16	-1.8341533680213473
2214	17	-1.9289192353883067
2214	18	-1.775297463769327
2214	19	-1.9405618871241757
2214	20	-2.0853695456270245
2214	21	-1.9554296840057077
2214	22	-1.7541791807460712
2214	23	-1.9945124569638537
2214	24	-1.9459715033012799
2214	25	-1.926742800381966
2215	1	-1.6384617730451971
2215	2	-1.3120020170718014
2215	3	-1.3998999486937684
2215	4	-2.1134218657072807
2215	5	-1.7727089561772438
2215	6	-1.528251235227799
2215	7	-1.5401505513523972
2215	8	-1.675161911994099
2215	9	-1.66849303572873
2215	10	-1.5920756881584097
2215	11	-1.7122368733979627
2215	12	-1.80885973321476
2215	13	-1.8371650101430674
2215	14	-1.8874027496568004
2215	15	-1.830361260033392
2215	16	-1.8582164267191743
2215	17	-1.8659705084834926
2215	18	-2.194811411300307
2215	19	-2.5014627464382784
2215	20	-2.2979002074573955
2215	21	-2.0899507253220975
2215	22	-3.0775323982155527
2215	23	-2.3510735877623716
2215	24	-2.3702034594877404
2215	25	-2.307363643655677
2216	1	-2.8322011639572366
2216	2	-5.482020334681103
2216	3	-3.1255831274124333
2216	4	-5.730670319229073
2216	5	-3.3549489510723127
2216	6	-2.397278737421029
2216	7	-2.219740261410312
2216	8	-2.125940676934345
2216	9	-2.4758749638500532
2216	10	-2.388308038701851
2216	11	-2.1876073988035216
2216	12	-2.3842947943379045
2216	13	-2.253023316091223
2216	14	-2.9444599702655196
2216	15	-2.754570818830601
2216	16	-2.832421275228519
2216	17	-2.587233694703613
2216	18	-2.5103128970301256
2216	19	-2.6328584142783242
2216	20	-2.848112313629194
2216	21	-3.104788569684324
2216	22	-3.0382241708225877
2216	23	-2.9586328916391125
2216	24	-2.943915048562225
2216	25	-3.209206297126329
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107342.0
3	3.0
4	718.0
5	4466.0
6	6370.0
7	5990.0
8	5442.0
9	4629.0
10	4444.0
11	4093.0
12	3844.0
13	3747.0
14	3626.0
15	3661.0
16	3810.0
17	3951.0
18	4641.0
19	5764.0
20	7648.0
21	9879.0
22	12305.0
23	14830.0
24	17315.0
25	19572.0
26	21871.0
27	24092.0
28	27222.0
29	31839.0
30	39845.0
31	50612.0
32	65004.0
33	85055.0
34	115719.0
35	162042.0
36	238648.0
37	422970.0
38	1260743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42795153935744	18.920250812619706	12.975520413264933	24.67627723475792
2	14.692569865096388	21.753570898671228	41.20115981682338	22.352699419409007
3	18.106756783462778	26.692142268119028	30.081115896020737	25.119985052397453
4	11.580198663074125	18.06007038948513	39.948117041744254	30.411613905696488
5	13.566018233936052	37.3272059986257	34.94844931040531	14.158326457032947
6	29.21040894651591	39.23326256940274	18.34834326508154	13.20798521899981
7	26.01234941556308	32.474692612564944	23.512092405662987	18.000865566208983
8	24.21540910002523	36.15835656968858	21.38154418789208	18.24469014239411
9	26.179416917479	16.03748259287543	20.471748798346884	37.31135169129868
10	14.597704205937271	28.8492497560036	33.89645353575571	22.656592502303415
11	33.499769088808264	23.575403735190687	24.929891049450934	17.994936126550115
12	23.036970986255966	25.419004151495255	31.787924759135283	19.756100103113496
13	28.2695569261534	22.618290736460533	26.28860736564761	22.823544971738457
14	21.570760118729687	22.304373861928216	27.95481715728808	28.17004886205402
15	22.690476164803247	30.028886682042703	24.536427518581178	22.744209634572872
16	22.022120344333846	28.94017285697035	26.830172051914598	22.207534746781217
17	22.133424291807053	27.738476656427846	27.290848825147666	22.83725022661743
18	22.76545790716198	26.962592770446882	28.989237046643296	21.28271227574784
19	24.33129082662033	26.573782754303586	27.300911310013987	21.794015109062094
20	23.231585507781865	27.49329856036759	27.771491870932678	21.503624060917865
21	23.265129495521148	26.97799695719041	27.24788187601827	22.508991671270177
22	22.586051901622927	27.496638295715677	27.907411421203843	22.009898381457553
23	22.372258182772242	26.93419638653378	27.69042219402883	23.003123236665147
24	22.30318017137271	27.33272471102421	28.183693714417164	22.180401403185922
25	22.631313723108068	27.098895316362793	28.055743858894942	22.2140471016342
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13846.0
1	13846.0
2	11331.5
3	8817.0
4	8817.0
5	8817.0
6	8046.5
7	7276.0
8	7276.0
9	7276.0
10	9205.0
11	11134.0
12	11134.0
13	11134.0
14	15491.5
15	19849.0
16	19849.0
17	19849.0
18	33213.5
19	46578.0
20	46578.0
21	46578.0
22	68319.0
23	90060.0
24	90060.0
25	90060.0
26	122647.5
27	155235.0
28	155235.0
29	155235.0
30	191058.5
31	226882.0
32	226882.0
33	226882.0
34	244957.0
35	263032.0
36	263032.0
37	263032.0
38	278588.0
39	294144.0
40	294144.0
41	294144.0
42	308922.5
43	323701.0
44	323701.0
45	323701.0
46	324731.5
47	325762.0
48	325762.0
49	325762.0
50	315365.5
51	304969.0
52	304969.0
53	304969.0
54	272470.5
55	239972.0
56	239972.0
57	239972.0
58	211187.0
59	182402.0
60	182402.0
61	182402.0
62	152521.0
63	122640.0
64	122640.0
65	122640.0
66	98832.0
67	75024.0
68	75024.0
69	75024.0
70	57508.0
71	39992.0
72	39992.0
73	39992.0
74	31831.5
75	23671.0
76	23671.0
77	23671.0
78	19114.5
79	14558.0
80	14558.0
81	14558.0
82	11144.0
83	7730.0
84	7730.0
85	7730.0
86	6018.0
87	4306.0
88	4306.0
89	4306.0
90	3130.0
91	1954.0
92	1954.0
93	1954.0
94	1068.5
95	183.0
96	183.0
97	183.0
98	109.0
99	35.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.411876656708582
2	0.7207841492400183
3	0.7381893976357395
4	0.7472130202671278
5	0.7578416350661542
6	0.7583052994701386
7	0.7760315462993873
8	0.7659022623969596
9	0.7535259894598382
10	0.746285691459159
11	0.7595892932042492
12	0.7625852785838405
13	0.7614439508201867
14	0.7716089012152287
15	0.7668652576975424
16	0.7609802864162023
17	0.7678995859833537
18	0.7709669043481735
19	0.7744265541317492
20	0.7673289221015268
21	0.76522459903729
22	0.7732852263680953
23	0.7626209450764547
24	0.7672575891162985
25	0.7703249074811181
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2803752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.0458474454437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5628800073946	61.62427917203195
2	5.729974145842855	7.7980189318871895
3	1.3377976061567345	2.7309471546426276
4	0.6152047483524866	1.6744851381642352
5	0.37910956660796447	1.2898415867256912
6	0.24640535896283147	1.0060116879434773
7	0.18048393684964953	0.8596827703257043
8	0.1230050655350912	0.6695987139534095
9	0.10052615492921896	0.6156348662331605
>10	0.6153643569166211	7.880263193104891
>50	0.05637694166994088	2.6545213278464144
>100	0.046877081601478056	6.308451231623555
>500	0.004207038021484448	1.948113119622124
>1k	0.001630227233264871	2.007013473983535
>5k	1.0517595053321748E-4	0.5672111287127679
>10k+	5.258797526660874E-5	0.36592650319959397
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10226	0.36472555347263236	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	9647	0.3440746542490206	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6204	0.2212749201783895	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	4347	0.15504224339385222	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3975	0.14177430814137626	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3617	0.12900570378549886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.566649261418271E-5	0.0
10	3.566649261418271E-5	0.0	0.0	3.566649261418271E-5	0.0
11	3.566649261418271E-5	0.0	0.0	7.133298522836542E-5	0.0
12	3.566649261418271E-5	0.0	0.0	1.0699947784254813E-4	0.0
13	3.566649261418271E-5	0.0	0.0	1.0699947784254813E-4	3.566649261418271E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1605	0.0	15.635087	1
GTATCAA	5255	0.0	13.16831	1
AGCCGTC	65	5.458512E-5	13.153075	7
TTTTCGG	175	0.0	11.9428005	16
GGGTATA	275	0.0	11.406507	1
TATCACG	75	2.0750292E-4	11.399946	2
ACCGAGC	85	5.3283395E-5	11.176017	8
ACTGTTC	1035	0.0	11.105828	8
AACCGCG	95	1.36204E-5	10.999355	7
TTGCGCA	70	0.0014927746	10.856506	4
TCGAACG	140	8.36917E-9	10.856506	3
CGAACGT	115	8.8308116E-7	10.738501	4
TATAACG	80	3.7728585E-4	10.687449	2
CGAACGA	215	0.0	10.604601	16
GTCTAAA	350	0.0	10.591756	1
GCGTTAT	145	1.4320904E-8	10.488741	1
TAGGACC	940	0.0	10.307906	4
TGGCGAG	1505	0.0	10.099619	18
ACGAACG	245	0.0	10.081223	15
TACGCTC	85	6.5967184E-4	10.058776	12
>>END_MODULE