FastQCFastQC Report
Thu 2 Feb 2017
SRR4050258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2803752
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172590.6155679960281794No Hit
TATCAACGCAGAGTACTTTTTTTTT118140.4213639437439545No Hit
GTACTTTTTTTTTTTTTTTTTTTTT67150.2395004979042369No Hit
GGTATCAACGCAGAGTACTTTTTTT65400.23325886169675492No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54020.192670393101815No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46330.1652428602815085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.015.91198351
GTATCAA58550.012.7937031
AACCGCG1207.512426E-1012.6568227
CCGACCA2200.012.5136649
CGGTCTA701.09890534E-412.20544510
CGACCAT2250.011.81366610
GTCTAAT2400.011.530291
CGAACGA1604.5474735E-1111.27288416
TATAACG1201.2521014E-711.0988672
AGAACCG1900.011.0148285
CGTCGTA1658.185452E-1110.93147710
CGGACGG700.001475376810.8717775
ATACCGT1855.456968E-1210.7796336
CGAGCTC1855.456968E-1210.7759810
GCGTTAT1252.1090091E-710.68738651
ACTGTTC11400.010.6589478
AATCGCT1909.094947E-1210.49240115
AGGCCCG2450.010.4618110
CGAGCCG2200.010.35613515
GATATAC11150.010.1841691
Produced by FastQC (version 0.11.5)