##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2803752
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8700719607155	31.0	31.0	34.0	27.0	34.0
2	30.985884985547937	31.0	31.0	34.0	27.0	34.0
3	31.138668469964532	33.0	31.0	34.0	27.0	34.0
4	34.69071800929611	37.0	35.0	37.0	32.0	37.0
5	34.58762258573511	37.0	35.0	37.0	32.0	37.0
6	34.60634963434712	37.0	35.0	37.0	32.0	37.0
7	34.50106179148512	37.0	35.0	37.0	31.0	37.0
8	34.48574873954615	37.0	35.0	37.0	31.0	37.0
9	36.08750738296397	39.0	37.0	39.0	31.0	39.0
10	35.98965065383814	39.0	37.0	39.0	31.0	39.0
11	35.96639003734995	39.0	37.0	39.0	31.0	39.0
12	35.886518137124824	39.0	37.0	39.0	30.0	39.0
13	35.884969498015515	39.0	37.0	39.0	30.0	39.0
14	37.067205302038126	40.0	37.0	41.0	31.0	41.0
15	37.05500878822378	40.0	37.0	41.0	31.0	41.0
16	37.040564572044886	40.0	37.0	41.0	31.0	41.0
17	37.052145482196714	40.0	37.0	41.0	31.0	41.0
18	37.04225124048061	40.0	37.0	41.0	31.0	41.0
19	37.09122383149437	40.0	38.0	41.0	31.0	41.0
20	37.07076695799058	40.0	37.0	41.0	31.0	41.0
21	37.00368256536242	40.0	37.0	41.0	31.0	41.0
22	36.94831211890352	40.0	37.0	41.0	31.0	41.0
23	36.86591039435728	40.0	37.0	41.0	31.0	41.0
24	36.80368912799705	40.0	37.0	41.0	31.0	41.0
25	36.54253050911778	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9035681098001156
1101	2	0.9712921218599426
1101	3	0.9299654886244149
1101	4	0.8610034186757787
1101	5	0.8465620881254878
1101	6	0.8199176719645607
1101	7	0.91841892429305
1101	8	0.9559679531641194
1101	9	1.0095491890025485
1101	10	1.0389024900160067
1101	11	1.0711174581840837
1101	12	1.112499307249955
1101	13	1.0916263156992798
1101	14	1.2420889504547787
1101	15	1.3774546364815592
1101	16	1.337843580908249
1101	17	1.2840903150786716
1101	18	1.280004224523573
1101	19	1.3016240419287826
1101	20	1.2798561399231474
1101	21	1.2373277752091099
1101	22	1.3184456231243828
1101	23	1.4061549822001425
1101	24	1.3969221763759236
1101	25	1.4227778827481004
1102	1	0.9144561739164452
1102	2	1.0036046491983974
1102	3	0.920237274804979
1102	4	0.8719892292258606
1102	5	0.9149939220951708
1102	6	0.8590171340703776
1102	7	0.925737022067473
1102	8	1.0308830415035715
1102	9	1.0875859429945436
1102	10	1.121174774703384
1102	11	1.170785497965987
1102	12	1.2088770687810921
1102	13	1.1910933088390365
1102	14	1.3154600894428796
1102	15	1.3390540613770838
1102	16	1.2792532577607645
1102	17	1.3349839951719176
1102	18	1.3044177292597183
1102	19	1.3317908128953349
1102	20	1.202773746566173
1102	21	1.206544313105958
1102	22	1.2609851598907014
1102	23	1.347325846742912
1102	24	1.4021642731879922
1102	25	1.311445361154469
1103	1	0.5911224295284825
1103	2	0.6868169322133753
1103	3	0.6304150225558622
1103	4	0.5953746892047747
1103	5	0.6303600927207853
1103	6	0.5727180064547852
1103	7	0.6522261904266671
1103	8	0.6795734109152036
1103	9	0.7016558862233353
1103	10	0.7890446017828907
1103	11	0.7832069364674723
1103	12	0.8051870629947047
1103	13	0.8641017403775422
1103	14	0.948879252388096
1103	15	0.9567575167304057
1103	16	0.9343812345252829
1103	17	0.9743040756824115
1103	18	0.9696946096853338
1103	19	0.9556801836901485
1103	20	0.9288118790852664
1103	21	1.005781578405184
1103	22	1.0085578471726393
1103	23	0.9937338441093502
1103	24	0.9923186364977497
1103	25	0.9296068789227476
1104	1	0.575229948686605
1104	2	0.5589352916927801
1104	3	0.5270700272008106
1104	4	0.483948981253711
1104	5	0.4163371745347888
1104	6	0.47788037328488997
1104	7	0.5774613253432008
1104	8	0.6304734924306388
1104	9	0.6488396094982392
1104	10	0.7188051248130805
1104	11	0.6747942040827439
1104	12	0.7048846670267395
1104	13	0.6550745300660523
1104	14	0.7503787502809729
1104	15	0.8431303927607061
1104	16	0.7580093270940651
1104	17	0.7616626926693826
1104	18	0.6845172711759346
1104	19	0.7329553846578207
1104	20	0.773918107110795
1104	21	0.7768775832168942
1104	22	0.8294746611540162
1104	23	0.9242543346398762
1104	24	0.8326654149405286
1104	25	0.7949905789645868
1105	1	0.4751265358744625
1105	2	0.5245340745962501
1105	3	0.5518884078577848
1105	4	0.42768363855715563
1105	5	0.37133862143055296
1105	6	0.423426222208775
1105	7	0.5061882180075159
1105	8	0.5310126465031075
1105	9	0.5603640632990334
1105	10	0.6122744652968493
1105	11	0.6859864556640005
1105	12	0.6501696013547118
1105	13	0.6189767369528951
1105	14	0.8375627881647105
1105	15	0.7379379461562081
1105	16	0.6820063064427444
1105	17	0.6690298062590614
1105	18	0.6484117150015081
1105	19	0.7258489016496128
1105	20	0.7058779045516985
1105	21	0.7271492430256643
1105	22	0.717718640915777
1105	23	0.6742917140954745
1105	24	0.7456245834472384
1105	25	0.666676643291332
1106	1	0.3127229920473873
1106	2	0.317731383930397
1106	3	0.33418906907562373
1106	4	0.24512717208046553
1106	5	0.16666621017679262
1106	6	0.20492309440810885
1106	7	0.2748227963194054
1106	8	0.3297184746294022
1106	9	0.3683634336245447
1106	10	0.39609218884470465
1106	11	0.46106869794914473
1106	12	0.4370006392979704
1106	13	0.39811268760931284
1106	14	0.45405399866024254
1106	15	0.43677620866010614
1106	16	0.3541600257259887
1106	17	0.46494163275034595
1106	18	0.4203719405633066
1106	19	0.4480841029576226
1106	20	0.5121993951152319
1106	21	0.49838134783784227
1106	22	0.44971826883540444
1106	23	0.5165503254605
1106	24	0.5161397776236996
1106	25	0.5027928277740301
1107	1	0.49496319342490125
1107	2	0.478803049926789
1107	3	0.4462958221958324
1107	4	0.4538828561369215
1107	5	0.3743043683625018
1107	6	0.3605185579284935
1107	7	0.4340209479360695
1107	8	0.4447336406845892
1107	9	0.4939655244727632
1107	10	0.4283699839365056
1107	11	0.43152188991776796
1107	12	0.5275236633480063
1107	13	0.5918994563361437
1107	14	0.5479246289695183
1107	15	0.6162420522078591
1107	16	0.5408447881176031
1107	17	0.5606646920455631
1107	18	0.4338393358588988
1107	19	0.5801726094980353
1107	20	0.5328546811548804
1107	21	0.5429616987879342
1107	22	0.5880327950260309
1107	23	0.6139938157480174
1107	24	0.6240796797943347
1107	25	0.49750279389682817
1108	1	0.2794902029206341
1108	2	0.3422937052661865
1108	3	0.2586302714351447
1108	4	0.24036406249690856
1108	5	0.18567076765068435
1108	6	0.16939247199388774
1108	7	0.30818875583896954
1108	8	0.31424326857089824
1108	9	0.36406591640675856
1108	10	0.3395652211681508
1108	11	0.3712228675992648
1108	12	0.37728615034803425
1108	13	0.4268993328477606
1108	14	0.4556535554010779
1108	15	0.4759731049499365
1108	16	0.4746287743084636
1108	17	0.4218724348829994
1108	18	0.47880143042438306
1108	19	0.5115338030218268
1108	20	0.5028934279056116
1108	21	0.5241005939612577
1108	22	0.5124531160551342
1108	23	0.55169851700321
1108	24	0.50072891672135
1108	25	0.4614244924677777
1109	1	0.2544232905485302
1109	2	0.38553336074775757
1109	3	0.3296202348182007
1109	4	0.24684631631983933
1109	5	0.2928343911858775
1109	6	0.28967300127125384
1109	7	0.23079106560171425
1109	8	0.3036133553778342
1109	9	0.42046812805294564
1109	10	0.4780271459024945
1109	11	0.3418048860677274
1109	12	0.4283351068165331
1109	13	0.3887027219988184
1109	14	0.3820130541764257
1109	15	0.5025917070299641
1109	16	0.4880395413746328
1109	17	0.5084585466019007
1109	18	0.44320949466705173
1109	19	0.5408546977828479
1109	20	0.5146057338475245
1109	21	0.5040347384495192
1109	22	0.5308773611431263
1109	23	0.4345885688100637
1109	24	0.44951599030527234
1109	25	0.4533184637349805
1110	1	0.10500929653482416
1110	2	0.1172210243259002
1110	3	0.10504705580284934
1110	4	0.08549800563046972
1110	5	-0.02879625472713343
1110	6	-0.01671519733840654
1110	7	0.13957802960339905
1110	8	0.22867327642351398
1110	9	0.15298092076039183
1110	10	0.08361842766586136
1110	11	0.11812175649199474
1110	12	0.1706431520860221
1110	13	0.15805458487109547
1110	14	0.27624294100269253
1110	15	0.18464528872756603
1110	16	0.1830191776365595
1110	17	0.03274665732870119
1110	18	0.11332425133410595
1110	19	0.1251423997521428
1110	20	0.26675601875551536
1110	21	0.2933348694522877
1110	22	0.19704840932962497
1110	23	0.29329923140999625
1110	24	0.21335213144202214
1110	25	0.17291371765955432
1111	1	-0.22946714095014187
1111	2	-0.2585208705343689
1111	3	-0.25258171254417405
1111	4	-0.31046690322385473
1111	5	-0.26136725363099345
1111	6	-0.2519318066941594
1111	7	-0.29381035175736514
1111	8	-0.21306113455914755
1111	9	-0.27181453174922865
1111	10	-0.25303738025471034
1111	11	-0.27002364391479716
1111	12	-0.27174618269355477
1111	13	-0.20821706493781988
1111	14	-0.1954031778015164
1111	15	-0.40039058043941367
1111	16	-0.21819019197088352
1111	17	-0.3071332066424546
1111	18	-0.2971948013615773
1111	19	-0.3283682922661626
1111	20	-0.3157389338711951
1111	21	-0.24307861196862746
1111	22	-0.2897799228897924
1111	23	-0.28716376732034377
1111	24	-0.4131565172140341
1111	25	-0.42644517062819176
1112	1	-0.18269488211220164
1112	2	-0.2984167408436704
1112	3	-0.21503100232816408
1112	4	-0.2542650010682763
1112	5	-0.26586259132527346
1112	6	-0.2600689805694927
1112	7	-0.2254885222568248
1112	8	-0.286375849458679
1112	9	-0.3874793813240629
1112	10	-0.3248657172567775
1112	11	-0.3847663431025907
1112	12	-0.37982555547018393
1112	13	-0.32063062508631646
1112	14	-0.43843742043259226
1112	15	-0.4326020730306155
1112	16	-0.3945097674254825
1112	17	-0.42766433948850135
1112	18	-0.41719683666195806
1112	19	-0.4298711566337161
1112	20	-0.42701701300589434
1112	21	-0.42678854747448725
1112	22	-0.5002008487849849
1112	23	-0.4632982951261795
1112	24	-0.49423226923246233
1112	25	-0.42920763890158753
1113	1	-0.5313401156399209
1113	2	-0.524986652693137
1113	3	-0.47642657535766375
1113	4	-0.5687989178190236
1113	5	-0.5789874672495543
1113	6	-0.5738107265042771
1113	7	-0.5288528177440668
1113	8	-0.67828682726973
1113	9	-0.7520402438754772
1113	10	-0.7080221167058127
1113	11	-0.6598260653056798
1113	12	-0.6326396820103213
1113	13	-0.7193105870559933
1113	14	-0.756427595422096
1113	15	-0.7212856277737103
1113	16	-0.8260551856442149
1113	17	-0.7534497866127197
1113	18	-0.7634545854934132
1113	19	-0.8607806947837133
1113	20	-0.729564641494747
1113	21	-0.943123163285037
1113	22	-0.8423156807641377
1113	23	-0.8114986422574972
1113	24	-0.9064083518744184
1113	25	-0.8650259823574586
1114	1	-0.7353149520060072
1114	2	-1.1894864261319853
1114	3	-0.84291774376306
1114	4	-0.7576696499135167
1114	5	-0.8251135685793187
1114	6	-0.7645067362228062
1114	7	-0.7427742744547103
1114	8	-0.882630464927658
1114	9	-0.860481849677555
1114	10	-0.9180401376405314
1114	11	-0.8929196612981372
1114	12	-0.8788362846161206
1114	13	-0.9010514908650222
1114	14	-1.0557918546001304
1114	15	-1.1889125886497496
1114	16	-1.0296942643241351
1114	17	-1.1623891173976162
1114	18	-1.1036333493374855
1114	19	-1.0604896057819744
1114	20	-1.1191298585990808
1114	21	-1.2101216438567164
1114	22	-1.1630390626340343
1114	23	-1.28489730209796
1114	24	-1.3334541759712195
1114	25	-1.2769481070357216
1115	1	-0.9201509160399084
1115	2	-1.232317143389313
1115	3	-1.1693728016247071
1115	4	-1.0075493198801695
1115	5	-1.2058765061548087
1115	6	-1.170603178325031
1115	7	-1.2930131924780213
1115	8	-1.3404710795721755
1115	9	-1.569000893437682
1115	10	-1.3392110617751811
1115	11	-1.4174227918146087
1115	12	-1.337493078843373
1115	13	-1.6447585204617
1115	14	-1.5489578829476898
1115	15	-1.5529206175341201
1115	16	-1.5836934152392885
1115	17	-1.561381893724409
1115	18	-1.5246474895681033
1115	19	-1.5819370631728873
1115	20	-1.6941825715412975
1115	21	-1.5702426578033837
1115	22	-1.7412029360094152
1115	23	-1.6575784488012388
1115	24	-1.6676622946676005
1115	25	-1.5405831450767877
1116	1	-0.4967585710078417
1116	2	-0.5144662208787416
1116	3	-0.5704421028332725
1116	4	-0.529180829844691
1116	5	-0.6712808421244674
1116	6	-0.6522498712254148
1116	7	-0.8192223484986272
1116	8	-0.882628920183528
1116	9	-0.8903528759005113
1116	10	-0.8521120800821294
1116	11	-0.8937128101725449
1116	12	-1.1166721197140674
1116	13	-1.0133211772387156
1116	14	-1.1160293064890752
1116	15	-1.1610053184041789
1116	16	-1.1806067292352722
1116	17	-1.2285982411507774
1116	18	-1.2505380528362053
1116	19	-1.2101948531542135
1116	20	-1.0926943433249576
1116	21	-1.1080717529465431
1116	22	-1.2152120233091708
1116	23	-1.2681367510321166
1116	24	-1.284913063224053
1116	25	-1.5459100729636148
1201	1	0.8018918251065195
1201	2	0.8781346610190539
1201	3	0.8310372116252402
1201	4	0.7554799232099896
1201	5	0.8721185596836065
1201	6	0.849046553525433
1201	7	0.8934120542738171
1201	8	0.8119723499764291
1201	9	0.9078729043089524
1201	10	0.9520654468722896
1201	11	0.8817797280384383
1201	12	0.924260780182081
1201	13	0.929219060958971
1201	14	1.1324434434473574
1201	15	1.1084246831976898
1201	16	1.116079359968431
1201	17	1.2280309581124698
1201	18	1.1887703690687772
1201	19	1.2351222557237804
1201	20	1.2183007675681026
1201	21	1.213145307498202
1201	22	1.214570932275251
1201	23	1.2657317890151987
1201	24	1.2911238801406952
1201	25	1.3172543872823113
1202	1	0.5643196358098983
1202	2	0.6771754548687099
1202	3	0.5888589719513675
1202	4	0.6046582256336492
1202	5	0.6918068447320636
1202	6	0.6905496920964254
1202	7	0.6804265278355999
1202	8	0.6713284506617043
1202	9	0.8167766656174393
1202	10	0.8814529008107002
1202	11	0.7831087251454534
1202	12	0.8041823645924353
1202	13	0.8335658898230065
1202	14	1.0101114451697342
1202	15	0.9651978730287638
1202	16	1.0076550935458428
1202	17	0.9692432839006813
1202	18	0.9818865016049756
1202	19	0.9622877182437719
1202	20	0.87929831599903
1202	21	0.978649588547988
1202	22	1.007340642739102
1202	23	1.0553681215717319
1202	24	1.0157743746738888
1202	25	1.1128387500366372
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2211	3	-0.45024128175381506
2211	4	-0.33070525463750755
2211	5	-0.3215762298691658
2211	6	-0.40118868545979325
2211	7	-0.3765473877671255
2211	8	-0.39816385252862574
2211	9	-0.3318071741995041
2211	10	-0.508292175191329
2211	11	-0.4865078750478702
2211	12	-0.4419023359798473
2211	13	-0.45699175045484
2211	14	-0.6117706125326094
2211	15	-0.5558314050095348
2211	16	-0.6019621132483124
2211	17	-0.6062908758701084
2211	18	-0.6072711825952695
2211	19	-0.5592815468649448
2211	20	-0.622843041090583
2211	21	-0.6379293175822198
2211	22	-0.6014719754567892
2211	23	-0.6554567263708506
2211	24	-0.6483234498196424
2211	25	-0.5330685197934528
2212	1	-0.49161454473391686
2212	2	-0.49349758482430417
2212	3	-0.5276837106841157
2212	4	-0.41677461785978664
2212	5	-0.429600954810887
2212	6	-0.4970133502822236
2212	7	-0.6111214684012154
2212	8	-0.588255391376876
2212	9	-0.5810392382797005
2212	10	-0.7342480085153653
2212	11	-0.6561801255184605
2212	12	-0.6668653457808631
2212	13	-0.6257625698257527
2212	14	-0.8129215749494563
2212	15	-0.816725831894189
2212	16	-0.785289761981872
2212	17	-0.7535984465703933
2212	18	-0.8025493422196348
2212	19	-0.8107724053613055
2212	20	-0.7554027136039068
2212	21	-0.7998681391089377
2212	22	-0.81409253028
2212	23	-0.8046951803083786
2212	24	-0.8127773765078743
2212	25	-0.6866147316491507
2213	1	-0.5722190146703916
2213	2	-0.5440275971356421
2213	3	-0.5407456213864386
2213	4	-0.5639940577013576
2213	5	-0.5767904835225153
2213	6	-0.5776239745950775
2213	7	-0.565299554018317
2213	8	-0.6397399775906081
2213	9	-0.7462556240012361
2213	10	-0.7148193308945991
2213	11	-0.7328992903067473
2213	12	-0.7391547367807902
2213	13	-0.7520330719686399
2213	14	-1.037189115933515
2213	15	-1.0908855680014113
2213	16	-0.9848296642469307
2213	17	-0.9493222481433108
2213	18	-0.8140757575346811
2213	19	-1.0197243718739273
2213	20	-0.9100763513283923
2213	21	-0.9269461126475278
2213	22	-0.9007324637128846
2213	23	-0.9282728630419257
2213	24	-0.9883087122253187
2213	25	-0.8488340221502568
2214	1	-1.1725562842792705
2214	2	-1.6850091536368517
2214	3	-1.196239571966668
2214	4	-1.1104466305163
2214	5	-1.1593088942819563
2214	6	-1.1557843311537184
2214	7	-1.1091116547645186
2214	8	-1.2216746830439575
2214	9	-1.4184939306403663
2214	10	-1.365018999085727
2214	11	-1.404907585277627
2214	12	-1.4981880572386004
2214	13	-1.4794129233813536
2214	14	-1.7509573442850552
2214	15	-1.734960386310597
2214	16	-1.7427642723603185
2214	17	-1.675801453976284
2214	18	-1.6745989565174
2214	19	-1.6480309934419708
2214	20	-1.690631128935415
2214	21	-1.817131994354881
2214	22	-1.8446797783716065
2214	23	-1.8007612263581692
2214	24	-1.7813763169236907
2214	25	-1.684737150150056
2215	1	-1.0139509502103756
2215	2	-1.1228708942451924
2215	3	-1.2344432717404352
2215	4	-0.9887758276843712
2215	5	-1.1164293234709532
2215	6	-1.1098623829944714
2215	7	-1.0613394107392864
2215	8	-1.12345164706079
2215	9	-1.3793735311129538
2215	10	-1.317544486251336
2215	11	-1.3754976759391866
2215	12	-1.5396282152728489
2215	13	-1.598893850717964
2215	14	-1.7498973766293915
2215	15	-1.8255495317675425
2215	16	-1.7455563026825445
2215	17	-1.6765974831584813
2215	18	-1.7310727426431214
2215	19	-1.7301375884790886
2215	20	-1.6946850569897265
2215	21	-1.5654685808913626
2215	22	-1.6907278419435627
2215	23	-1.6618295331311046
2215	24	-1.691199913568532
2215	25	-1.6654118882637263
2216	1	-0.9163009541966147
2216	2	-0.9189198061289154
2216	3	-1.0308203124607118
2216	4	-0.9179797237926053
2216	5	-0.8790661823964001
2216	6	-0.9081746598028175
2216	7	-1.0547378070454059
2216	8	-1.008068491400259
2216	9	-1.2745363280500541
2216	10	-1.2489684237750964
2216	11	-1.228199972856956
2216	12	-1.2960743476411665
2216	13	-1.2018587541403534
2216	14	-1.4226641710732224
2216	15	-1.5214870039692912
2216	16	-1.3666660783280378
2216	17	-1.3736638331325537
2216	18	-1.5120615434563476
2216	19	-1.5327019742153851
2216	20	-1.631061122234854
2216	21	-1.5940173412024734
2216	22	-1.6810224211183282
2216	23	-1.632000362222925
2216	24	-1.677198821609231
2216	25	-1.6184854863523697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	38108.0
3	5.0
4	704.0
5	5635.0
6	7326.0
7	5880.0
8	4733.0
9	3908.0
10	3505.0
11	3246.0
12	2932.0
13	2750.0
14	2662.0
15	2761.0
16	2922.0
17	3045.0
18	3554.0
19	4372.0
20	6178.0
21	8261.0
22	10665.0
23	12649.0
24	15008.0
25	17486.0
26	18879.0
27	21398.0
28	24312.0
29	29318.0
30	37646.0
31	49728.0
32	64793.0
33	85918.0
34	117695.0
35	162753.0
36	238236.0
37	418695.0
38	1366086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74001158803773	19.709947903994863	13.537866618604138	25.012173889363265
2	14.621987874991929	22.421349202301162	40.21023481368955	22.746428109017355
3	17.69874796344327	27.03124242087032	30.48736122167724	24.782648394009172
4	11.712965572047878	18.67524327702228	39.23745734938797	30.37433380154188
5	13.214702200270962	37.65641702823367	34.868671148382525	14.260209623112848
6	28.959985498061652	39.77252938926286	18.094165539055318	13.173319573620173
7	25.737497873348214	32.67161322008786	23.49346560602175	18.097423300542175
8	24.270518760840435	36.234506849603335	21.171407181624254	18.32356720793198
9	25.980232881212178	15.879107494363792	20.563766987534894	37.576892636889134
10	14.38536382322823	29.232691427668534	33.73270087952169	22.649243869581547
11	33.458370129842336	23.583447349010665	24.920498140319893	18.037684380827105
12	22.957015249891928	25.6479670377488	31.749635837317363	19.64538187504191
13	28.408531565708806	22.543454657777183	26.3941301435434	22.65388363297061
14	21.532160000456578	22.220022564916942	28.302633845877267	27.945183588749217
15	22.571100020829945	30.011206795698186	24.85700813509218	22.560685048379685
16	21.835915960643913	28.888831822134343	26.96354219052164	22.31171002670011
17	22.019931800995877	27.66800068484356	27.530318594929305	22.781748919231262
18	22.62724414211854	26.7069318739514	29.47369659997489	21.192127383955167
19	24.157327616185444	26.29954606882477	27.964649935853288	21.5784763791365
20	23.051102331283264	27.13945290583807	28.534884359632752	21.274560403245914
21	23.07542777287742	26.559030748856223	28.016524179738617	22.34901729852774
22	22.423843818480645	27.157389917366693	28.541804579072316	21.87696168508035
23	22.17325118468537	26.588562676864626	28.430879502713918	22.807306635736087
24	22.032697096738065	27.104435109074736	28.895941539565584	21.96692625462161
25	22.39345262192958	26.759500849810863	28.74700604409029	22.100040484169263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	786.0
1	786.0
2	1486.0
3	2186.0
4	2186.0
5	2186.0
6	5866.5
7	9547.0
8	9547.0
9	9547.0
10	11233.0
11	12919.0
12	12919.0
13	12919.0
14	16626.0
15	20333.0
16	20333.0
17	20333.0
18	34222.0
19	48111.0
20	48111.0
21	48111.0
22	70782.0
23	93453.0
24	93453.0
25	93453.0
26	128880.5
27	164308.0
28	164308.0
29	164308.0
30	199158.0
31	234008.0
32	234008.0
33	234008.0
34	250288.5
35	266569.0
36	266569.0
37	266569.0
38	279902.0
39	293235.0
40	293235.0
41	293235.0
42	308242.0
43	323249.0
44	323249.0
45	323249.0
46	324024.0
47	324799.0
48	324799.0
49	324799.0
50	313954.5
51	303110.0
52	303110.0
53	303110.0
54	270786.5
55	238463.0
56	238463.0
57	238463.0
58	210134.0
59	181805.0
60	181805.0
61	181805.0
62	151790.0
63	121775.0
64	121775.0
65	121775.0
66	98238.5
67	74702.0
68	74702.0
69	74702.0
70	57000.0
71	39298.0
72	39298.0
73	39298.0
74	31199.0
75	23100.0
76	23100.0
77	23100.0
78	18646.0
79	14192.0
80	14192.0
81	14192.0
82	10915.0
83	7638.0
84	7638.0
85	7638.0
86	5893.5
87	4149.0
88	4149.0
89	4149.0
90	2993.0
91	1837.0
92	1837.0
93	1837.0
94	999.5
95	162.0
96	162.0
97	162.0
98	90.0
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46163141390536677
2	0.004458311576772839
3	0.0
4	0.005635305833040868
5	0.1720551603708174
6	0.04879176189620195
7	0.0017476581380949526
8	4.636644039843752E-4
9	1.7833246307091355E-4
10	0.002460987990378607
11	0.0015693256750240392
12	0.0021399895568509626
13	0.004993308965985579
14	0.010164950395042071
15	0.00342398329096154
16	6.419968670552887E-4
17	0.00684796658192308
18	2.8533194091346167E-4
19	8.203293301262024E-4
20	0.0036736487392608186
21	0.0036736487392608186
22	0.0014266597045673084
23	0.0019259906011658665
24	0.0027819864239062514
25	0.006669634118852167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2803752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.5211319736685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36330686246987	61.01432768237933
2	5.860349272474585	7.913948332770973
3	1.383924843828699	2.803325160653885
4	0.6453568293589971	1.7430089458102844
5	0.37510319781573687	1.2663696261730726
6	0.2504196658336036	1.014517158333163
7	0.17687849335792497	0.836012526532687
8	0.12706852645056588	0.6863848595334596
9	0.09929783880165298	0.6034232230583825
>10	0.6059321150300775	7.730578351964154
>50	0.05813688118080939	2.765007177602095
>100	0.048261708522189875	6.483223310396847
>500	0.004380464466029474	2.020100233507737
>1k	0.0013194170078011816	1.4188951982031806
>5k	1.583300409361418E-4	0.6648498810276199
>10k+	1.0555336062409453E-4	1.03602833205317
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17259	0.6155679960281794	No Hit
TATCAACGCAGAGTACTTTTTTTTT	11814	0.4213639437439545	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6715	0.2395004979042369	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6540	0.23325886169675492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5402	0.192670393101815	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4633	0.1652428602815085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.566649261418271E-5	0.0
5	0.0	0.0	0.0	3.566649261418271E-5	0.0
6	0.0	0.0	0.0	3.566649261418271E-5	3.566649261418271E-5
7	0.0	0.0	0.0	3.566649261418271E-5	3.566649261418271E-5
8	0.0	0.0	0.0	3.566649261418271E-5	3.566649261418271E-5
9	0.0	0.0	0.0	7.133298522836542E-5	3.566649261418271E-5
10	0.0	0.0	0.0	1.0699947784254813E-4	3.566649261418271E-5
11	0.0	0.0	0.0	1.0699947784254813E-4	3.566649261418271E-5
12	0.0	0.0	0.0	1.4266597045673083E-4	7.133298522836542E-5
13	0.0	0.0	0.0	1.4266597045673083E-4	7.133298522836542E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1955	0.0	15.9119835	1
GTATCAA	5855	0.0	12.793703	1
AACCGCG	120	7.512426E-10	12.656822	7
CCGACCA	220	0.0	12.513664	9
CGGTCTA	70	1.09890534E-4	12.205445	10
CGACCAT	225	0.0	11.813666	10
GTCTAAT	240	0.0	11.53029	1
CGAACGA	160	4.5474735E-11	11.272884	16
TATAACG	120	1.2521014E-7	11.098867	2
AGAACCG	190	0.0	11.014828	5
CGTCGTA	165	8.185452E-11	10.931477	10
CGGACGG	70	0.0014753768	10.871777	5
ATACCGT	185	5.456968E-12	10.779633	6
CGAGCTC	185	5.456968E-12	10.77598	10
GCGTTAT	125	2.1090091E-7	10.6873865	1
ACTGTTC	1140	0.0	10.658947	8
AATCGCT	190	9.094947E-12	10.492401	15
AGGCCCG	245	0.0	10.46181	10
CGAGCCG	220	0.0	10.356135	15
GATATAC	1115	0.0	10.184169	1
>>END_MODULE