FastQCFastQC Report
Thu 2 Feb 2017
SRR4050257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8799921
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT152330.1731038267275354No Hit
GTACAAAGGGCAGGGACTTAATCAA140720.159910526469499No Hit
GAATAGGACCGCGGTTCTATTTTGT104920.11922834307262532No Hit
GTATCTGATCGTCTTCGAACCTCCG104880.11918288811910925No Hit
GATTAAGAGGGACGGCCGGGGGCAT99850.1134669277144647No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG98380.11179645817274951No Hit
CTATTGGAGCTGGAATTACCGCGGC98140.11152372845165315No Hit
GTCCTATTCCATTATTCCTAGCTGC91160.10359183906310067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.015.5957131
GTATCAA160050.013.4433711
ACGCTTA4850.012.15419417
CGAACGA8650.011.87165416
CTCGACG5200.011.33735613
ATCGCCA14950.011.25733616
CGCTTAC5250.011.04718718
GACGCTT5700.010.84281416
CGACCAT15350.010.78074610
GTATTAG13750.010.7395561
TAGATCG3100.010.7372225
TCGACGC5700.010.67612514
GGTAGCG4450.010.47234711
CGAGCCG17800.010.4699515
CCGACCA16450.010.4067359
CGATAAC10250.010.39207110
CGGTTCT20250.010.37709412
CGCCAGT15900.010.34499318
CGTCGTA14000.010.25786110
CGGTAGC4400.010.1590510
Produced by FastQC (version 0.11.5)