##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050257_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8799921
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.531438407231157	31.0	31.0	34.0	27.0	34.0
2	30.665324836438874	31.0	31.0	34.0	27.0	34.0
3	30.772662845496	31.0	31.0	34.0	27.0	34.0
4	34.301739867891996	37.0	35.0	37.0	30.0	37.0
5	34.29658811709787	37.0	35.0	37.0	30.0	37.0
6	34.34224818609167	37.0	35.0	37.0	31.0	37.0
7	34.2783321577546	37.0	35.0	37.0	30.0	37.0
8	34.23302527374962	37.0	35.0	37.0	30.0	37.0
9	35.805854279828196	39.0	37.0	39.0	30.0	39.0
10	35.68912084551668	39.0	37.0	39.0	30.0	39.0
11	35.579830091656504	39.0	37.0	39.0	30.0	39.0
12	35.497761173083255	39.0	37.0	39.0	30.0	39.0
13	35.50645556931704	39.0	37.0	39.0	30.0	39.0
14	36.663677662560836	40.0	37.0	41.0	30.0	41.0
15	36.61755145301873	40.0	37.0	41.0	30.0	41.0
16	36.621575920965654	40.0	37.0	41.0	30.0	41.0
17	36.59184269949696	40.0	37.0	41.0	30.0	41.0
18	36.557990691052794	40.0	37.0	41.0	30.0	41.0
19	36.58222079493668	40.0	37.0	41.0	30.0	41.0
20	36.53719709529211	40.0	37.0	41.0	30.0	41.0
21	36.49036951581724	40.0	37.0	41.0	30.0	41.0
22	36.4130157532096	40.0	37.0	41.0	30.0	41.0
23	36.33832996909859	40.0	37.0	41.0	30.0	41.0
24	36.27050072381331	40.0	37.0	41.0	30.0	41.0
25	36.03816693354406	40.0	37.0	41.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.544961822005618
1101	2	0.55558989966352
1101	3	0.5639785950679794
1101	4	0.5993612807615065
1101	5	0.5852438866740286
1101	6	0.6114307307330762
1101	7	0.6242806142455279
1101	8	0.5919728002903923
1101	9	0.6571809235478057
1101	10	0.6792083655235004
1101	11	0.5643866504026533
1101	12	0.614092649057433
1101	13	0.5121831257163336
1101	14	0.7185987796766184
1101	15	0.7349413332706618
1101	16	0.783680715055894
1101	17	0.7931569678889687
1101	18	0.7828954888100341
1101	19	0.8268226182379621
1101	20	0.7109867048590885
1101	21	0.7847795040205057
1101	22	0.7729794035661612
1101	23	0.7913752746124771
1101	24	0.8275523347374261
1101	25	0.8669448533949762
1102	1	0.4344307088150785
1102	2	0.472506428605282
1102	3	0.47232363233119656
1102	4	0.4270544149194677
1102	5	0.36945359235369324
1102	6	0.3899764560605803
1102	7	0.3708920208551447
1102	8	0.4573260971848683
1102	9	0.5233232329716202
1102	10	0.529464329636717
1102	11	0.4450830088669946
1102	12	0.39699830179466034
1102	13	0.4221967249440155
1102	14	0.5571457318100315
1102	15	0.49248650273483463
1102	16	0.5565734134329503
1102	17	0.5594132693270808
1102	18	0.5841710305088625
1102	19	0.6397235509811807
1102	20	0.6067321961526417
1102	21	0.6496157480168137
1102	22	0.6549467616190583
1102	23	0.6259599115568975
1102	24	0.5449917546532461
1102	25	0.6152192580397369
1103	1	0.5861201827457982
1103	2	0.5718421802821823
1103	3	0.5826876192451991
1103	4	0.597288638131289
1103	5	0.6333541451828921
1103	6	0.5776472568136484
1103	7	0.5924962344885998
1103	8	0.6234135813730077
1103	9	0.6585602661752361
1103	10	0.6679811705072893
1103	11	0.6426995465183722
1103	12	0.5623219900288348
1103	13	0.5414200540003122
1103	14	0.7453569625644718
1103	15	0.7529642279085209
1103	16	0.7692745959624432
1103	17	0.6578022529786764
1103	18	0.742661863316016
1103	19	0.7561540631630166
1103	20	0.7789029939511849
1103	21	0.7173926248324776
1103	22	0.792107195223906
1103	23	0.8133767272676593
1103	24	0.8323929190389379
1103	25	0.9021961056574312
1104	1	0.5084986715742374
1104	2	0.5319616412869728
1104	3	0.5151870776532483
1104	4	0.5248416201397461
1104	5	0.5567525030742786
1104	6	0.5385928731896854
1104	7	0.5461946393043675
1104	8	0.5583299306726843
1104	9	0.6479078071122046
1104	10	0.5772406396683536
1104	11	0.5490785347620886
1104	12	0.5333134462402498
1104	13	0.5222872842850279
1104	14	0.6134185127795817
1104	15	0.5234140468659518
1104	16	0.5895684518407265
1104	17	0.6351785233413807
1104	18	0.6804709795838235
1104	19	0.7059603449410545
1104	20	0.6807682538469209
1104	21	0.6853812651281714
1104	22	0.7214341524955401
1104	23	0.7077246999379057
1104	24	0.7072062159164076
1104	25	0.8115980422688409
1105	1	0.35365098340109213
1105	2	0.36156379366085645
1105	3	0.37831834397180586
1105	4	0.41961939033358675
1105	5	0.4523083672989472
1105	6	0.3352477381320469
1105	7	0.35848086197088236
1105	8	0.3797404062588896
1105	9	0.3978163528230141
1105	10	0.3564517929750579
1105	11	0.32428126264156276
1105	12	0.20660997122514146
1105	13	0.29568909612547145
1105	14	0.3856269437486972
1105	15	0.37347248005271183
1105	16	0.2597251745963476
1105	17	0.3497361463602573
1105	18	0.4503463855641101
1105	19	0.44627587972311744
1105	20	0.3689001420776563
1105	21	0.3643617399185075
1105	22	0.37413694743528936
1105	23	0.3910336127052716
1105	24	0.3473024038204642
1105	25	0.38368203768413167
1106	1	0.28013100370225175
1106	2	0.30868045090896956
1106	3	0.3105173714710432
1106	4	0.33054296881498146
1106	5	0.34434272072823546
1106	6	0.3287419785757564
1106	7	0.306385033441984
1106	8	0.286789394446771
1106	9	0.28537678233536923
1106	10	0.3727379396421213
1106	11	0.29680141775976665
1106	12	0.3504397037287035
1106	13	0.3099932746657572
1106	14	0.3536622722032945
1106	15	0.3789038832980225
1106	16	0.304789545330415
1106	17	0.39181745865248274
1106	18	0.39095766418831346
1106	19	0.2788752227197975
1106	20	0.3782764060986139
1106	21	0.3611004334542045
1106	22	0.3884104782334674
1106	23	0.1927532315422411
1106	24	0.32882446454139824
1106	25	0.41403511127433035
1107	1	0.33587660289373744
1107	2	0.3220079065724022
1107	3	0.2576857742756786
1107	4	0.38955741400963717
1107	5	0.33080045707040995
1107	6	0.19747140322939316
1107	7	0.2755578500866065
1107	8	0.2781433556027437
1107	9	0.3335209711660241
1107	10	0.37064876233539934
1107	11	0.2786195244702583
1107	12	0.265989401921253
1107	13	0.31586865340268844
1107	14	0.35281370893175534
1107	15	0.3982360679351018
1107	16	0.38549907448035725
1107	17	0.3660688524790885
1107	18	0.4369559466461368
1107	19	0.45429324495462
1107	20	0.37760517663596715
1107	21	0.38786919552168797
1107	22	0.4069718645083995
1107	23	0.32668547549504723
1107	24	0.38149922322337915
1107	25	0.41873112857354045
1108	1	0.2729273094146194
1108	2	0.23458244140461204
1108	3	0.23639073549558276
1108	4	0.2708147630132416
1108	5	0.2894542801348692
1108	6	0.23343543052251192
1108	7	0.22816054058055357
1108	8	0.18247602120855078
1108	9	0.15211410998457353
1108	10	0.11456527158266283
1108	11	0.19790658645828785
1108	12	0.13016933097506467
1108	13	0.18734977138696252
1108	14	0.10352642416207658
1108	15	0.26029458510475934
1108	16	0.20342772965371125
1108	17	0.16209782457040234
1108	18	0.05096149351206236
1108	19	0.14738991182932892
1108	20	0.2320983007160109
1108	21	0.25384648736111615
1108	22	0.3314032241229157
1108	23	0.20795214204345314
1108	24	0.2610418194091082
1108	25	0.19811543450288838
1109	1	-0.006094012119280734
1109	2	0.02094777968724415
1109	3	0.07664685017150674
1109	4	0.07993897106691605
1109	5	0.08572019041848478
1109	6	0.004021709679221885
1109	7	0.05092948788588103
1109	8	0.058426303074611496
1109	9	0.08018665261007385
1109	10	-0.09885054357253864
1109	11	-0.019698332504233917
1109	12	0.006859533815060104
1109	13	-0.06089049727717821
1109	14	-0.01800223044705973
1109	15	0.03146823680555855
1109	16	0.002573389721241881
1109	17	-0.036630518889680275
1109	18	0.009216529505650328
1109	19	-0.017796234726752402
1109	20	-0.00704896228818086
1109	21	0.033941075023768974
1109	22	0.07435824167738048
1109	23	0.0701373989555023
1109	24	0.04246722412688797
1109	25	0.06363570837357457
1110	1	-0.05222527037797775
1110	2	-0.04142243942022361
1110	3	0.01654686971032504
1110	4	-0.032941181268611786
1110	5	-2.6420169082541634E-4
1110	6	0.04658722729161724
1110	7	0.08089698919251731
1110	8	-0.007665369466778316
1110	9	0.041899836977677296
1110	10	-0.082616999795313
1110	11	-0.04724750363624963
1110	12	-0.12352934093963341
1110	13	-0.06544868278983529
1110	14	-0.1176099597488971
1110	15	-0.05855008036809295
1110	16	-0.030690903969208705
1110	17	0.017830402169387582
1110	18	-0.02130597880021412
1110	19	-0.08098484258793093
1110	20	-0.04389438857995742
1110	21	-0.06012132889325983
1110	22	-0.02777331993732446
1110	23	-0.017644564288360698
1110	24	-0.03460716870345237
1110	25	0.012909690029502485
1111	1	-0.05828008825078257
1111	2	-0.09595369501790785
1111	3	-0.09871993741931107
1111	4	-0.014934625023492742
1111	5	-0.06027166305116083
1111	6	-0.04590890033448858
1111	7	-0.013313683468950899
1111	8	-0.0035721728586821655
1111	9	-0.08991649941913948
1111	10	-0.06339069378876161
1111	11	-0.2124615254435227
1111	12	-0.22147505237030884
1111	13	-0.16925158560236042
1111	14	-0.17506955234357235
1111	15	-0.16400122417550023
1111	16	-0.1932601957287048
1111	17	-0.18956148708577558
1111	18	-0.11683340361101813
1111	19	-0.117514496321391
1111	20	-0.12383097250454256
1111	21	-0.11238894981131153
1111	22	-0.08276298119485404
1111	23	-0.07019478873670693
1111	24	-0.2054280915870237
1111	25	-0.12180839276306443
1112	1	-0.08327234830005636
1112	2	-0.08393905527891832
1112	3	-0.07021280032701682
1112	4	-0.14046230994909337
1112	5	-0.054082234802748985
1112	6	-0.12065851599181343
1112	7	-0.14993348361076642
1112	8	-0.08872886703790783
1112	9	-0.03883777186704407
1112	10	-0.10160712425418694
1112	11	-0.15228601236034933
1112	12	-0.12596820098226402
1112	13	-0.11695231989700261
1112	14	-0.1713919780645483
1112	15	-0.201632890131485
1112	16	-0.13219146884644317
1112	17	-0.12055569090420448
1112	18	-0.13324993099941196
1112	19	-0.2947164969863323
1112	20	-0.1675327969273397
1112	21	-0.20587992184292858
1112	22	-0.14214967791458832
1112	23	-0.15043726260467594
1112	24	-0.1784414872157285
1112	25	-0.1339865057753329
1113	1	-0.3332879721997841
1113	2	-0.3415017929382387
1113	3	-0.22727062195929548
1113	4	-0.16518350035821783
1113	5	-0.19314726543439775
1113	6	-0.25557562130130407
1113	7	-0.2817742210588321
1113	8	-0.30001592428740054
1113	9	-0.3201037808628868
1113	10	-0.42256422014532546
1113	11	-0.3031749242327493
1113	12	-0.4933349907329827
1113	13	-0.4206046815264628
1113	14	-0.40625742548022714
1113	15	-0.37160458006528785
1113	16	-0.3710017308878548
1113	17	-0.34375245081547945
1113	18	-0.33044698138319006
1113	19	-0.4250889729563312
1113	20	-0.3553111664083559
1113	21	-0.39953238249710665
1113	22	-0.4163080228374838
1113	23	-0.36974493680015286
1113	24	-0.47251749858816794
1113	25	-0.41742665743718277
1114	1	-0.4063218725174096
1114	2	-0.4562136451514327
1114	3	-0.47265391215094965
1114	4	-0.39498218584424194
1114	5	-0.3961730396038732
1114	6	-0.4120454536170399
1114	7	-0.48753699319910737
1114	8	-0.4212258015502428
1114	9	-0.4824896002171144
1114	10	-0.5366060013613136
1114	11	-0.6125282471166571
1114	12	-0.6208330992918221
1114	13	-0.5098557060757614
1114	14	-0.7801020072261906
1114	15	-0.7250387328482617
1114	16	-0.6705786677886962
1114	17	-0.7169053804840715
1114	18	-0.7468713721104479
1114	19	-0.5725939716960582
1114	20	-0.6860478881334942
1114	21	-0.6769782937766493
1114	22	-0.8297295198444559
1114	23	-0.6971649187154227
1114	24	-0.6712802848334931
1114	25	-0.7036038047251623
1115	1	-0.41124818052335144
1115	2	-0.46387684857347367
1115	3	-0.4838340791523734
1115	4	-0.35508247058896103
1115	5	-0.4231996931610311
1115	6	-0.45058007879351436
1115	7	-0.5137924341356381
1115	8	-0.5280430160559817
1115	9	-0.46448835685219336
1115	10	-0.5562412068379885
1115	11	-0.6454186998855533
1115	12	-0.6625992872261364
1115	13	-0.607034973204506
1115	14	-0.6334312898458236
1115	15	-0.5643292886423978
1115	16	-0.6534147381794426
1115	17	-0.6405427790697686
1115	18	-0.5294950160242848
1115	19	-0.5749943191062385
1115	20	-0.5997674340975294
1115	21	-0.6812524880327899
1115	22	-0.8245763790993763
1115	23	-0.7283796504653353
1115	24	-0.6195400548078993
1115	25	-0.6127952009659126
1116	1	-0.5446881348562478
1116	2	-0.7097648306411486
1116	3	-0.6931853536250117
1116	4	-0.7146424751406286
1116	5	-0.6863920142402549
1116	6	-0.7281518575811461
1116	7	-0.670362299461658
1116	8	-0.7549729322423531
1116	9	-0.8136214982814138
1116	10	-0.8123952322828245
1116	11	-0.8687467878147075
1116	12	-0.9408341488835106
1116	13	-0.9135801679078881
1116	14	-0.9507506218677975
1116	15	-1.0188306925296615
1116	16	-0.9685166931479401
1116	17	-1.0311128802986573
1116	18	-0.925634419378575
1116	19	-0.9978371159478883
1116	20	-1.0483404320072722
1116	21	-1.0853973578960847
1116	22	-1.137919806890828
1116	23	-1.0338368937337137
1116	24	-1.1040797752460207
1116	25	-1.0635240571769558
1201	1	0.46495480315802595
1201	2	0.534954238534926
1201	3	0.529270200586069
1201	4	0.5791449562153801
1201	5	0.5514455001741396
1201	6	0.5868723898176924
1201	7	0.6062442007184004
1201	8	0.6022793792669461
1201	9	0.6762298802901228
1201	10	0.6557001914879805
1201	11	0.6200627399936991
1201	12	0.6433159630450191
1201	13	0.6935430825740383
1201	14	0.7591027199751679
1201	15	0.789399030415801
1201	16	0.7034494120672079
1201	17	0.6923237211542315
1201	18	0.7379551458995195
1201	19	0.7261751971442436
1201	20	0.6818909960875601
1201	21	0.7137888260170229
1201	22	0.7211657236290989
1201	23	0.7169829226518445
1201	24	0.7172408838109234
1201	25	0.681313697885571
1202	1	0.4611904985035338
1202	2	0.4152985497698438
1202	3	0.4583551790627389
1202	4	0.5411562747640843
1202	5	0.5607743174576711
1202	6	0.5146723675413867
1202	7	0.5283902551798718
1202	8	0.4806330107844943
1202	9	0.5283455981917413
1202	10	0.6285775682775423
1202	11	0.5441177730512479
1202	12	0.5209094687098315
1202	13	0.46888902659982534
1202	14	0.5981878725932077
1202	15	0.56562469625284
1202	16	0.6326936363004094
1202	17	0.5469358307333465
1202	18	0.6554258370693304
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2210	13	0.05514156007387072
2210	14	-0.016390077701778694
2210	15	0.016470976276501403
2210	16	0.09960380406779024
2210	17	-0.026021868136915316
2210	18	-0.006634663644817351
2210	19	0.07722198169024352
2210	20	0.017792753460362576
2210	21	-0.029327661661739057
2210	22	0.021616830846348023
2210	23	-0.008696060853793597
2210	24	0.10094035573025906
2210	25	0.03518668881853415
2211	1	-0.118527969801697
2211	2	-0.1690369490253012
2211	3	-0.13804860261743812
2211	4	-0.20667117406798496
2211	5	-0.17436387283916588
2211	6	-0.1748962556495428
2211	7	-0.14537224623702372
2211	8	-0.1602409625149761
2211	9	-0.1051347483998839
2211	10	-0.20358555706960146
2211	11	-0.015558968835932774
2211	12	-0.09838419009311394
2211	13	-0.025808899225317816
2211	14	-0.04333679446099836
2211	15	0.01877536237768851
2211	16	-0.08082384153833289
2211	17	-0.14125361360252242
2211	18	-0.10302279901073774
2211	19	-0.16925924734018594
2211	20	-0.16046269302204053
2211	21	-0.13129675940646024
2211	22	-0.11089862237511738
2211	23	-0.03439462082059208
2211	24	-0.20163209150705086
2211	25	-0.20257551087409098
2212	1	-0.28728883289873863
2212	2	-0.20609639476445452
2212	3	-0.2926840849871901
2212	4	-0.21391687381860436
2212	5	-0.22210096042293515
2212	6	-0.2348681355706077
2212	7	-0.2862022328501439
2212	8	-0.34858463780839344
2212	9	-0.2423813771093819
2212	10	-0.3255174235454561
2212	11	-0.13813015648069182
2212	12	-0.1455368413731719
2212	13	-0.16611629037220155
2212	14	-0.18210651183760262
2212	15	-0.23637867650664646
2212	16	-0.20688719653391274
2212	17	-0.20549497955801854
2212	18	-0.335866207508964
2212	19	-0.29878986281807585
2212	20	-0.30875908565481325
2212	21	-0.29948072557620264
2212	22	-0.27466958509514683
2212	23	-0.2452429756882637
2212	24	-0.36797887320305733
2212	25	-0.29213401306925846
2213	1	-0.29990868137426574
2213	2	-0.3760739335161567
2213	3	-0.3877424495361623
2213	4	-0.3268117427468269
2213	5	-0.3584984662852193
2213	6	-0.22170212544508416
2213	7	-0.27472271248839775
2213	8	-0.341282687777543
2213	9	-0.26695419585190905
2213	10	-0.23729730098964552
2213	11	-0.14194566945296572
2213	12	-0.2876400400325494
2213	13	-0.1061963708558693
2213	14	-0.34088413397385864
2213	15	-0.30545720024959166
2213	16	-0.16125133286053028
2213	17	-0.3116726534491079
2213	18	-0.25777507150696266
2213	19	-0.26293619950684644
2213	20	-0.30257432619490743
2213	21	-0.19766656292880924
2213	22	-0.31578309406614125
2213	23	-0.227486646150318
2213	24	-0.25914439524401445
2213	25	-0.22281551384530474
2214	1	-0.47355725768129275
2214	2	-0.5251104529976978
2214	3	-0.5371736858521068
2214	4	-0.6672291866902711
2214	5	-0.5895856260571435
2214	6	-0.5423281354640395
2214	7	-0.5596082393817667
2214	8	-0.5282108050564034
2214	9	-0.5746636729971542
2214	10	-0.5641585924465105
2214	11	-0.4557865347154504
2214	12	-0.45251784961834574
2214	13	-0.5653703232728162
2214	14	-0.6354561526508391
2214	15	-0.4606071331605719
2214	16	-0.5461667986675565
2214	17	-0.5802540935708791
2214	18	-0.6624084933762546
2214	19	-0.6936104737648847
2214	20	-0.7115340717363097
2214	21	-0.6808152017372393
2214	22	-0.5999524730355645
2214	23	-0.5562390284583216
2214	24	-0.6725057978923132
2214	25	-0.564770772926181
2215	1	-0.6254952555247968
2215	2	-0.6017613859907307
2215	3	-0.79267930925797
2215	4	-0.7452435349557547
2215	5	-0.7421620801773159
2215	6	-0.6824266278286615
2215	7	-0.6928899452210047
2215	8	-0.759246728941406
2215	9	-0.896746704485011
2215	10	-0.8340153570587745
2215	11	-0.7513866688519499
2215	12	-0.7783940124325426
2215	13	-0.7638952184573853
2215	14	-0.9464655001253348
2215	15	-0.917857105340758
2215	16	-0.9426901275374604
2215	17	-0.9218440973291564
2215	18	-0.9315049796531056
2215	19	-0.9911988933473026
2215	20	-0.9692814972739114
2215	21	-0.9093100488090755
2215	22	-1.0343790417508956
2215	23	-0.86805568935754
2215	24	-0.9671982354162054
2215	25	-0.9267461448715721
2216	1	-0.6216375541930041
2216	2	-0.7058986645065595
2216	3	-0.7060694680415622
2216	4	-0.619477828984877
2216	5	-0.6675115914864875
2216	6	-0.6021120035210359
2216	7	-0.6429676863020859
2216	8	-0.614926119560451
2216	9	-0.6172143438761637
2216	10	-0.6786119223060467
2216	11	-0.5441977955806507
2216	12	-0.6122415293229082
2216	13	-0.6511491073596929
2216	14	-0.6417602690325452
2216	15	-0.681253277874653
2216	16	-0.837597527328775
2216	17	-0.9275795539301512
2216	18	-0.8004225330607824
2216	19	-0.8072297687415144
2216	20	-0.70555849768364
2216	21	-0.758383447942748
2216	22	-0.7788092653580492
2216	23	-0.9252994185214263
2216	24	-1.030094576601833
2216	25	-0.9863912972652287
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	225444.0
3	10.0
4	1884.0
5	10782.0
6	15142.0
7	15484.0
8	13033.0
9	12609.0
10	12604.0
11	14753.0
12	24306.0
13	21015.0
14	9947.0
15	9903.0
16	10433.0
17	10830.0
18	12233.0
19	14722.0
20	18633.0
21	23397.0
22	29360.0
23	35367.0
24	42069.0
25	48621.0
26	55480.0
27	63082.0
28	73251.0
29	88860.0
30	112623.0
31	146462.0
32	189717.0
33	252736.0
34	348962.0
35	493156.0
36	740970.0
37	1379313.0
38	4222728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80055853079544	17.679832577321463	12.941163082343463	25.578445809539634
2	16.589232590536067	20.604585628573897	37.85265759235435	24.953524188535688
3	18.598576822769925	24.367043577545626	28.525175392762534	28.50920420692191
4	12.975700598749215	16.584807890049756	37.19450121253918	33.244990298661854
5	15.61113265360258	35.28306036858836	32.98174545184624	16.124061525962826
6	32.60891888102393	36.69730508590027	16.445256526115205	14.248519506960594
7	29.022870360811027	30.685571879543794	21.311514180160373	18.980043579484803
8	26.685268980244135	32.50756571784634	20.07919627999211	20.727969021917414
9	27.40136970630664	14.972489203436615	18.809433975566527	38.81670711469022
10	16.30023234192289	26.341606306891475	30.76125411874728	26.596907232438355
11	36.39200978035513	22.230807638024324	22.194713797242564	19.18246878437798
12	24.9744451485819	23.88647651904946	28.359204665304656	22.77987366706398
13	29.35751166961083	20.403056410258756	24.63789518163207	25.601536738498336
14	23.906247247501675	20.40481772141068	25.09671063724354	30.592224393844102
15	24.904992897488416	26.883160767505938	22.863120909249602	25.348725425756047
16	25.116067780105894	26.465410062685297	23.670185383559055	24.748336773649754
17	24.778163914541416	25.318888836651567	24.69214391466267	25.210803334144344
18	26.15420361134862	24.44329866007293	25.432851379360915	23.969646349217534
19	26.977112700425888	24.717816804824487	24.565841868505817	23.739228626243815
20	25.94989616279989	24.793792808606067	24.580512698249084	24.675798330344957
21	26.343693595845323	24.806257680459264	24.46865205859472	24.38139666510069
22	25.223439367630927	24.69193902393965	25.24495653497878	24.83966507345064
23	24.56388542906912	24.039010754606707	25.242970847158315	26.154132969165854
24	24.666784789351752	25.49965266668825	24.992922361710953	24.840640182249043
25	24.902744118781424	24.676583876592204	25.125255692723115	25.295416311903256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5138.0
1	5138.0
2	5281.0
3	5424.0
4	5424.0
5	5424.0
6	9505.5
7	13587.0
8	13587.0
9	13587.0
10	18835.5
11	24084.0
12	24084.0
13	24084.0
14	33651.0
15	43218.0
16	43218.0
17	43218.0
18	66779.0
19	90340.0
20	90340.0
21	90340.0
22	131434.0
23	172528.0
24	172528.0
25	172528.0
26	237723.5
27	302919.0
28	302919.0
29	302919.0
30	371330.0
31	439741.0
32	439741.0
33	439741.0
34	524884.0
35	610027.0
36	610027.0
37	610027.0
38	674737.0
39	739447.0
40	739447.0
41	739447.0
42	822588.5
43	905730.0
44	905730.0
45	905730.0
46	965929.5
47	1026129.0
48	1026129.0
49	1026129.0
50	1047786.0
51	1069443.0
52	1069443.0
53	1069443.0
54	997353.0
55	925263.0
56	925263.0
57	925263.0
58	860051.0
59	794839.0
60	794839.0
61	794839.0
62	704985.0
63	615131.0
64	615131.0
65	615131.0
66	517230.5
67	419330.0
68	419330.0
69	419330.0
70	331424.5
71	243519.0
72	243519.0
73	243519.0
74	199858.0
75	156197.0
76	156197.0
77	156197.0
78	131259.5
79	106322.0
80	106322.0
81	106322.0
82	80529.0
83	54736.0
84	54736.0
85	54736.0
86	40161.0
87	25586.0
88	25586.0
89	25586.0
90	17895.0
91	10204.0
92	10204.0
93	10204.0
94	5523.5
95	843.0
96	843.0
97	843.0
98	519.5
99	196.0
100	196.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14296719254638762
2	0.16100144535388441
3	0.17444474785625916
4	0.19445629114170457
5	0.1846266574438566
6	0.06886425457683086
7	0.05633005114477732
8	0.060409633222843706
9	0.06726196746538975
10	0.06801197419840473
11	0.6998699192867754
12	0.6584263654185077
13	0.6564831661556962
14	0.6575399938249447
15	0.6584490928952658
16	0.6645173291896598
17	0.6564604386789382
18	0.6670400791098011
19	0.6630514069387669
20	0.6685741837909681
21	0.6564604386789382
22	0.6598241052391266
23	0.644221692444739
24	0.6545286031545056
25	0.6477899062957497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8799921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.48762189700183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.36720251095194	39.93789772064818
2	11.527407748213635	11.178731766959041
3	2.639783556268531	3.839904808988141
4	1.02689838705031	1.991674428717459
5	0.523286248869034	1.2686452889531061
6	0.3235036971313528	0.9411554969272339
7	0.22688818661399188	0.7700888023804942
8	0.16283199726852693	0.6316269053031975
9	0.12953841471211575	0.5652908706328279
>10	0.8664843731869749	8.196124587078245
>50	0.09935466360774302	3.333031021393439
>100	0.08236120864383838	8.139555956280509
>500	0.011640935251502463	3.866412388717698
>1k	0.011828441662303945	11.750845812346554
>5k	8.953800378241605E-4	3.014510021438477
>10k+	9.425053029728005E-5	0.5745041232354144
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	15233	0.1731038267275354	No Hit
GTACAAAGGGCAGGGACTTAATCAA	14072	0.159910526469499	No Hit
GAATAGGACCGCGGTTCTATTTTGT	10492	0.11922834307262532	No Hit
GTATCTGATCGTCTTCGAACCTCCG	10488	0.11918288811910925	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	9985	0.1134669277144647	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	9838	0.11179645817274951	No Hit
CTATTGGAGCTGGAATTACCGCGGC	9814	0.11152372845165315	No Hit
GTCCTATTCCATTATTCCTAGCTGC	9116	0.10359183906310067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.1363738379014994E-5	0.0	0.0	0.0	2.2727476758029988E-5
7	2.2727476758029988E-5	0.0	0.0	0.0	3.409121513704498E-5
8	2.2727476758029988E-5	0.0	0.0	1.1363738379014994E-5	3.409121513704498E-5
9	2.2727476758029988E-5	0.0	0.0	1.1363738379014994E-5	3.409121513704498E-5
10	2.2727476758029988E-5	0.0	0.0	3.409121513704498E-5	3.409121513704498E-5
11	2.2727476758029988E-5	0.0	0.0	4.5454953516059976E-5	3.409121513704498E-5
12	3.409121513704498E-5	0.0	0.0	4.5454953516059976E-5	5.681869189507497E-5
13	3.409121513704498E-5	0.0	0.0	4.5454953516059976E-5	1.0227364541113494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4710	0.0	15.595713	1
GTATCAA	16005	0.0	13.443371	1
ACGCTTA	485	0.0	12.154194	17
CGAACGA	865	0.0	11.871654	16
CTCGACG	520	0.0	11.337356	13
ATCGCCA	1495	0.0	11.257336	16
CGCTTAC	525	0.0	11.047187	18
GACGCTT	570	0.0	10.842814	16
CGACCAT	1535	0.0	10.780746	10
GTATTAG	1375	0.0	10.739556	1
TAGATCG	310	0.0	10.737222	5
TCGACGC	570	0.0	10.676125	14
GGTAGCG	445	0.0	10.472347	11
CGAGCCG	1780	0.0	10.46995	15
CCGACCA	1645	0.0	10.406735	9
CGATAAC	1025	0.0	10.392071	10
CGGTTCT	2025	0.0	10.377094	12
CGCCAGT	1590	0.0	10.344993	18
CGTCGTA	1400	0.0	10.257861	10
CGGTAGC	440	0.0	10.15905	10
>>END_MODULE