FastQCFastQC Report
Thu 2 Feb 2017
SRR4050257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8799921
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT247760.2815479820784755No Hit
TATCAACGCAGAGTACTTTTTTTTT149760.17018334596412854No Hit
GTACAAAGGGCAGGGACTTAATCAA138900.15784232608451826No Hit
GATTAAGAGGGACGGCCGGGGGCAT109670.12462611880265743No Hit
GAATAGGACCGCGGTTCTATTTTGT108990.1238533845928844No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG99190.11271692098144973No Hit
CTATTGGAGCTGGAATTACCGCGGC97780.11111463387000861No Hit
GGTATCAACGCAGAGTACTTTTTTT94520.10741005515844972No Hit
GTATCTGATCGTCTTCGAACCTCCG91910.1044441194415268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53850.016.6185131
GTATCAA173000.013.20733451
TCGACGC4200.012.42935614
ACGCTTA4350.012.00034817
TAGATCG3500.011.6728695
CTCGACG4400.011.64866913
CGTCGTA13850.011.380177510
AACCGCG8800.011.1146217
GAGCGTT5400.010.9027997
CGCTTAC4800.010.67758318
GAACGCG1355.6597855E-810.56064219
CGCCAGT14500.010.53848718
ATCGCCA14800.010.51755716
CCGTCGT15350.010.5157119
CGACCAT13650.010.50352410
GTATTAG12900.010.4058951
TCGCCAG15250.010.331387517
GACGCTT5150.010.32080416
CGAGCCG19800.010.2584415
GTCGTAG15150.010.21564311
Produced by FastQC (version 0.11.5)