FastQCFastQC Report
Thu 2 Feb 2017
SRR4050256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238625
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA597534.824139671006156No Hit
CTTCCGTACTCTGCGTTGATACCAC402863.2524775456655566No Hit
GAGTACGGAAGCAGTGGTATCAACG280102.2613785447572914No Hit
TCCGTACTCTGCGTTGATACCACTG279582.2571803411040468No Hit
GCTTCCGTACTCTGCGTTGATACCA164981.331960843677465No Hit
GCGTTGATACCACTGCTTCCGTACT150411.2143304067009788No Hit
GTACATGGGAAGCAGTGGTATCAAC129061.0419618528610353No Hit
GTACATGGAAGCAGTGGTATCAACG126081.0179029165405187No Hit
GTATCAACGCAGAGTACGGAAGCAG121840.983671409829448No Hit
CCCATGTACTCTGCGTTGATACCAC118690.9582399838530629No Hit
TCCATGTACTCTGCGTTGATACCAC104870.8466646482995256No Hit
TATCAACGCAGAGTACGGAAGCAGT91610.7396104551417904No Hit
ACTCTGCGTTGATACCACTGCTTCC87120.7033605812897366No Hit
GGTATCAACGCAGAGTACGGAAGCA67860.5478655767484105No Hit
ATACCACTGCTTCCGTACTCTGCGT67640.5460894136643455No Hit
CAGTGGTATCAACGCAGAGTACGGA64800.5231607629427792No Hit
ACGCAGAGTACGGAAGCAGTGGTAT60140.48553839943485716No Hit
CATGTACTCTGCGTTGATACCACTG49270.39777979614491876No Hit
GTGGTATCAACGCAGAGTACGGAAG46780.37767685942072865No Hit
GTACTCTGCGTTGATACCACTGCTT45170.3646785750327985No Hit
GAAGCAGTGGTATCAACGCAGAGTA44200.3568473105257846No Hit
GTATCAACGCAGAGTACTTTTTTTT43860.3541023312140478No Hit
AAGCAGTGGTATCAACGCAGAGTAC43800.35361792310021195No Hit
GAGTACATGGAAGCAGTGGTATCAA40000.3229387425572712No Hit
GAGTACATGGGAAGCAGTGGTATCA39470.31865980421838735No Hit
GTTGATACCACTGCTTCCGTACTCT39210.3165607023917651No Hit
GATACCACTGCTTCCGTACTCTGCG36190.29217882732869105No Hit
GTATCAACGCAGAGTACATGGGAAG35360.28547784842062773No Hit
CCACTGCTTCCGTACTCTGCGTTGA33920.27385205368856597No Hit
ATCAACGCAGAGTACGGAAGCAGTG32660.2636794832980119No Hit
GCGTTGATACCACTGCTTCCCATGT30700.2478554849127056No Hit
TATCAACGCAGAGTACATGGGAAGC28120.2270259360177616No Hit
CGGAAGCAGTGGTATCAACGCAGAG27390.2211323039660914No Hit
CTGCTTCCGTACTCTGCGTTGATAC26820.2165304268846503No Hit
TATCAACGCAGAGTACTTTTTTTTT25990.20982944797658695No Hit
GTACGGGAAGCAGTGGTATCAACGC25910.2091835704914724No Hit
AAAAAGTACTCTGCGTTGATACCAC25340.2045816934100313No Hit
CATGGAAGCAGTGGTATCAACGCAG24160.1950550005045918No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23470.18948430719547885No Hit
GCTTCCATGTACTCTGCGTTGATAC23430.18916136845292159No Hit
GCGTTGATACCACTGCTTCCATGTA23420.18908063376728226No Hit
GCAGAGTACGGAAGCAGTGGTATCA22980.1855283075991523No Hit
CAGTGGTATCAACGCAGAGTACATG22810.1841558179432839No Hit
CACTGCTTCCGTACTCTGCGTTGAT22430.1810878998889898No Hit
ACGCAGAGTACATGGGAAGCAGTGG21650.17479059440912303No Hit
TGATACCACTGCTTCCGTACTCTGC20250.16348773841961853No Hit
CCCGTACTCTGCGTTGATACCACTG19620.15840145322434152No Hit
GTATCAACGCAGAGTACATGGAAGC19250.15541426985568674No Hit
TTCCCGTACTCTGCGTTGATACCAC18830.1520234130588354No Hit
GGTATCAACGCAGAGTACATGGGAA18690.15089312745988495No Hit
GGTATCAACGCAGAGTACTTTTTTT18600.15016651528913108No Hit
GCTTCCCATGTACTCTGCGTTGATA18250.14734080129175497No Hit
GCAGTGGTATCAACGCAGAGTACGG17800.14370774043798568No Hit
TACCACTGCTTCCGTACTCTGCGTT16750.1352305984458573No Hit
CTGCGTTGATACCACTGCTTCCGTA16210.13087092542133416No Hit
TATCAACGCAGAGTACATGGAAGCA15740.12707639519628622No Hit
AAAGTACTCTGCGTTGATACCACTG15580.12578464022605712No Hit
ACGCAGAGTACATGGAAGCAGTGGT13670.11036431526894742No Hit
GAGTACGGGAAGCAGTGGTATCAAC13350.10778080532848926No Hit
CCATGTACTCTGCGTTGATACCACT13340.10770007064284993No Hit
GTATCAACGCAGAGTACGGGAAGCA13150.10616611161570289No Hit
TCAACGCAGAGTACGGAAGCAGTGG12540.1012412957917045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT509.0074085E-515.1406094
GTCTATA400.005367976614.2138521
TTTCGTT655.423133E-513.16095617
TTCGTTC655.4251985E-513.16042218
GCCTTAG655.5821536E-513.1204791
AGGCCCG905.3114127E-712.68432610
GTCGTAG1051.9534127E-812.68432611
CGTCAAT905.369275E-712.67299919
CGGAATT604.0737446E-412.67299815
CGTCACT604.0737446E-412.67299815
CGACTTT701.0850595E-412.22088713
TCGTTCT701.0854707E-412.22039119
CGTCGTA1103.7372956E-812.10776610
TATTTGA550.003159101612.0436682
TCCGTCA951.0324202E-612.00648617
CCGTCAA951.0329277E-612.00599918
CCGTCGT1209.798896E-911.8915569
TCGGAAT657.9877884E-411.69815314
CTTTCGT907.4189174E-611.61644516
CCTATTC1407.239578E-1011.4906413
Produced by FastQC (version 0.11.5)