##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050256_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1238625
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.384131597537593	31.0	31.0	34.0	27.0	34.0
2	30.49339953577556	31.0	31.0	34.0	26.0	34.0
3	30.614490261378545	31.0	31.0	34.0	27.0	34.0
4	34.111547078413565	37.0	35.0	37.0	30.0	37.0
5	34.11636370975881	37.0	35.0	37.0	30.0	37.0
6	34.09882006256938	37.0	35.0	37.0	30.0	37.0
7	33.95612392774245	37.0	35.0	37.0	30.0	37.0
8	33.88485296195378	37.0	35.0	37.0	30.0	37.0
9	35.28471329094762	39.0	35.0	39.0	29.0	39.0
10	35.24961307901907	39.0	35.0	39.0	29.0	39.0
11	35.083877686951254	39.0	35.0	39.0	28.0	39.0
12	35.01483580583308	39.0	35.0	39.0	27.0	39.0
13	34.97761792310021	39.0	35.0	39.0	27.0	39.0
14	36.12106973458472	40.0	37.0	41.0	27.0	41.0
15	36.09081360379453	40.0	37.0	41.0	27.0	41.0
16	35.99778867696034	40.0	36.0	41.0	27.0	41.0
17	36.06687657684933	40.0	37.0	41.0	27.0	41.0
18	36.00413038651731	40.0	36.0	41.0	27.0	41.0
19	36.06846220607529	40.0	37.0	41.0	27.0	41.0
20	36.05404137652639	40.0	37.0	41.0	27.0	41.0
21	35.99227369058432	40.0	37.0	41.0	27.0	41.0
22	35.9484953072964	40.0	37.0	41.0	27.0	41.0
23	35.851394893531136	40.0	36.0	41.0	27.0	41.0
24	35.77555272984156	40.0	36.0	41.0	27.0	41.0
25	35.567469573115346	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7770351010584235
1101	2	0.6775050216248175
1101	3	0.7198828752488531
1101	4	0.7368039388636873
1101	5	0.7186130939439792
1101	6	0.6734045407599751
1101	7	0.766222921636114
1101	8	0.8758311627937161
1101	9	0.9019748490403856
1101	10	0.9289645538850451
1101	11	0.7168092532671011
1101	12	0.8486348740693259
1101	13	0.8695955823701524
1101	14	1.0307646813009583
1101	15	1.0338246412140037
1101	16	1.0738500900024945
1101	17	1.0952720412209018
1101	18	1.101930457293662
1101	19	1.05652750644267
1101	20	1.043861763947362
1101	21	1.0429351878148623
1101	22	1.0931405232449052
1101	23	1.1819872884770604
1101	24	1.0224450285749427
1101	25	1.0502575589964493
1102	1	0.5582178147517531
1102	2	0.47421713676367006
1102	3	0.4149668492045251
1102	4	0.5595049138006516
1102	5	0.5840112966649542
1102	6	0.5655964994729317
1102	7	0.5953972484142938
1102	8	0.6747399737949991
1102	9	0.7948320725161366
1102	10	0.6910780642417933
1102	11	0.7475304254229798
1102	12	0.7108386450208997
1102	13	0.8351430585078461
1102	14	0.8782855462031947
1102	15	0.8524227738935366
1102	16	0.9438380713802417
1102	17	0.9127695686481587
1102	18	0.9362872707431649
1102	19	1.0556390893697127
1102	20	0.8415970904736909
1102	21	0.9428046179154705
1102	22	0.9582694598112838
1102	23	1.0070745742452019
1102	24	0.9715759760919056
1102	25	1.0145996256899679
1103	1	0.6754474213170809
1103	2	0.7909757795744348
1103	3	0.6661028732377581
1103	4	0.6768678297078026
1103	5	0.5877949662705788
1103	6	0.660354719824511
1103	7	0.4976270505625777
1103	8	0.5345154445631692
1103	9	0.7157495912725409
1103	10	0.6598993248912848
1103	11	0.6437459734855366
1103	12	0.6963933573966017
1103	13	0.570023635555799
1103	14	0.7793540550005105
1103	15	0.7263800429296197
1103	16	0.7651694436684835
1103	17	0.6963772135946869
1103	18	0.8728955493784696
1103	19	0.9034865033718873
1103	20	0.9081613238276276
1103	21	0.9527912400413783
1103	22	0.88548568850252
1103	23	0.9240885701614303
1103	24	0.8976744090812758
1103	25	1.0011507158693504
1104	1	0.49639987454821366
1104	2	0.4692651583927834
1104	3	0.6029540856234448
1104	4	0.4587795665119643
1104	5	0.5061860412905048
1104	6	0.46809273826643505
1104	7	0.5782432991870223
1104	8	0.522916006169055
1104	9	0.7172279808297048
1104	10	0.6704696584396501
1104	11	0.6550099661998345
1104	12	0.6995368325227744
1104	13	0.7005954869579156
1104	14	0.6402852582790786
1104	15	0.8217908121020159
1104	16	0.9187291901869017
1104	17	0.8545269498675978
1104	18	0.8974817444985703
1104	19	0.9629787812909143
1104	20	0.8982558102238585
1104	21	0.9261255852772123
1104	22	1.1301879994348596
1104	23	1.2003045607507303
1104	24	1.1588560582293752
1104	25	1.0424697307822655
1105	1	0.5707295738943401
1105	2	0.7070599466117748
1105	3	0.7015495300030139
1105	4	0.6386401323894759
1105	5	0.7385260373571967
1105	6	0.6385533160353347
1105	7	0.6609759396692638
1105	8	0.5524709786740374
1105	9	0.7841411142556325
1105	10	0.7747214387173287
1105	11	0.8122284185816966
1105	12	0.7926341879090373
1105	13	0.6909223018806898
1105	14	0.9392837901502773
1105	15	0.8936263694802378
1105	16	0.9090715553835622
1105	17	0.9206942799618005
1105	18	0.9782071800205756
1105	19	0.9542038532109274
1105	20	0.9637739926102782
1105	21	1.0221073640151133
1105	22	1.0803536403424445
1105	23	1.073030752442925
1105	24	1.232167062182505
1105	25	1.2042505115584703
1106	1	0.3839537578492056
1106	2	0.4384609454963453
1106	3	0.44549485917285736
1106	4	0.29516246013781
1106	5	0.3487223708563221
1106	6	0.4416776998259735
1106	7	0.47281275124385047
1106	8	0.5316321393108083
1106	9	0.36093455940674346
1106	10	0.45031174888952563
1106	11	0.4873310588272659
1106	12	0.4637642459761935
1106	13	0.3955397149638955
1106	14	0.5538423708748113
1106	15	0.4751837935706007
1106	16	0.48902264123642425
1106	17	0.540208434750646
1106	18	0.6192320815006553
1106	19	0.8073030735865245
1106	20	0.7898344613267128
1106	21	0.5930564220793215
1106	22	0.6732946402276667
1106	23	0.6009430130593856
1106	24	0.5546401991332814
1106	25	0.6106122937757377
1107	1	0.3334178533959289
1107	2	0.45287540117544367
1107	3	0.33797902823468107
1107	4	0.2763571377363121
1107	5	0.3321225013971514
1107	6	0.24047821282852766
1107	7	0.2570213188445294
1107	8	0.16541186883937087
1107	9	0.1550230065434306
1107	10	0.3360123752564874
1107	11	0.5158215946172078
1107	12	0.5553071469598336
1107	13	0.46878342552969343
1107	14	0.618321524420594
1107	15	0.6996052343291126
1107	16	0.619678551644192
1107	17	0.610515549225795
1107	18	0.633862960132042
1107	19	0.5577559586533454
1107	20	0.7244037338478648
1107	21	0.6092595161259737
1107	22	0.7500314630471223
1107	23	0.7508416721531574
1107	24	0.7830969546211506
1107	25	0.8448076927840802
1108	1	0.273834508768676
1108	2	0.28179394037438854
1108	3	0.2481923478346033
1108	4	0.19161206414511867
1108	5	0.365658673570735
1108	6	0.23172429383757276
1108	7	0.13804436748067417
1108	8	0.24117488621394045
1108	9	0.1728312628522204
1108	10	0.1901039373414335
1108	11	0.2832887744796935
1108	12	0.21744156270265336
1108	13	0.27177902541765775
1108	14	0.32873068062909283
1108	15	0.3434062263872235
1108	16	0.4711702528702588
1108	17	0.45564199597721
1108	18	0.412852199283094
1108	19	0.4718611637961416
1108	20	0.4923237999850727
1108	21	0.4741345149965781
1108	22	0.46892913107809164
1108	23	0.4547726346663694
1108	24	0.5462837380217991
1108	25	0.5039661600633281
1109	1	-0.007498831237420234
1109	2	0.021484802472880205
1109	3	0.06533367024240277
1109	4	-0.01256942161187169
1109	5	0.12184599132094576
1109	6	0.0591519555463762
1109	7	0.027809241621284286
1109	8	-0.0799425677273291
1109	9	-0.016673484817317785
1109	10	-0.022064843478382556
1109	11	-0.1855599962864929
1109	12	-0.06577127168608854
1109	13	-0.04940487810311822
1109	14	-0.08270759966691088
1109	15	0.022648267451394588
1109	16	0.04918039101865901
1109	17	0.05839250811162344
1109	18	0.014613474581203434
1109	19	0.08515185818590965
1109	20	0.04709966193899362
1109	21	0.08271924460517965
1109	22	0.13974208468683003
1109	23	0.14567449169578595
1109	24	0.13860305633252068
1109	25	0.14438162841331348
1110	1	-0.1790734070504385
1110	2	-0.16230698426791434
1110	3	-0.13932498111088876
1110	4	-0.048547686058938666
1110	5	-0.18026465151380222
1110	6	-0.3099564087351041
1110	7	-0.31166390161139645
1110	8	-0.1878266426201307
1110	9	-0.23937414507440025
1110	10	-0.2741142195065791
1110	11	-0.09570552046435665
1110	12	-0.17343628604642447
1110	13	-0.19269393710863625
1110	14	-0.1563810019953209
1110	15	-0.030040646628854972
1110	16	-0.07788631830905501
1110	17	-0.17269585043974445
1110	18	-0.13915083247260185
1110	19	-0.2436342604985029
1110	20	-0.13829596084308804
1110	21	-0.20081765495448423
1110	22	-0.2133298076346648
1110	23	-0.26571471358388976
1110	24	-0.1750441450612641
1110	25	-0.18725578431993029
1111	1	-0.18936307289851229
1111	2	-0.15866387937738935
1111	3	-0.20311884265223767
1111	4	-0.10684511449500178
1111	5	-0.0955104073818589
1111	6	-0.20614884486192864
1111	7	-0.3288223004639548
1111	8	-0.2649249930185178
1111	9	-0.16876037671097777
1111	10	-0.24308892651072966
1111	11	-0.09809438664806436
1111	12	-0.21751728687466
1111	13	-0.25739741234446
1111	14	-0.2595406501781383
1111	15	-0.03348276331877997
1111	16	-0.15515749789278743
1111	17	-0.06808020033075479
1111	18	-0.052570902461958724
1111	19	-0.07126956973357323
1111	20	-0.11603871351341866
1111	21	-0.17490903586742945
1111	22	-0.2639155409858418
1111	23	-0.4374686895593456
1111	24	-0.29645806863511126
1111	25	-0.3537863921361506
1112	1	0.026939937237386857
1112	2	-0.022398506315362
1112	3	0.07629241531992292
1112	4	0.05863602458737205
1112	5	0.0019790207101806345
1112	6	-0.06874384615347395
1112	7	-0.2874064281523303
1112	8	-0.4667169543693319
1112	9	-0.3630163716927015
1112	10	-0.26367965552656614
1112	11	-0.14774217416208302
1112	12	-0.24607458016439665
1112	13	-0.3319733476058744
1112	14	-0.19161030944993485
1112	15	-0.14739653357316485
1112	16	-0.20901870602676098
1112	17	-0.1346085826936445
1112	18	-0.11725918303070415
1112	19	-0.0843014442907446
1112	20	-0.10035735395083378
1112	21	-0.2942457742442386
1112	22	-0.2699491935199916
1112	23	-0.3613054275588823
1112	24	-0.12790564975389884
1112	25	-0.18793024050934548
1113	1	-0.4145154668562512
1113	2	-0.2925689456343221
1113	3	-0.34687043899277015
1113	4	-0.23379390690737978
1113	5	-0.36662106822159046
1113	6	-0.5652922812124359
1113	7	-0.4391447662796395
1113	8	-0.4096710969420414
1113	9	-0.5094081339907888
1113	10	-0.6063242738643737
1113	11	-0.3190445084224507
1113	12	-0.26589093011089915
1113	13	-0.5703608538837059
1113	14	-0.5008623396946774
1113	15	-0.3754777314522997
1113	16	-0.44449409396182205
1113	17	-0.5494452499756903
1113	18	-0.5235982016448375
1113	19	-0.5708708616884195
1113	20	-0.49428041606213213
1113	21	-0.4554740549430676
1113	22	-0.3868460803126297
1113	23	-0.6095838633920323
1113	24	-0.5475750042244059
1113	25	-0.6069740453596992
1114	1	-0.18079265638663955
1114	2	-0.08894383726583754
1114	3	-0.22445009591520204
1114	4	-0.1074145831267046
1114	5	-0.02083818508420876
1114	6	-0.21477406069597293
1114	7	-0.19412630710898782
1114	8	-0.20665480027054173
1114	9	-0.43161149657955633
1114	10	-0.19236178640589685
1114	11	-0.11060443010491383
1114	12	-0.011388402324733704
1114	13	0.16048617365406415
1114	14	-0.04988263991189967
1114	15	-0.03566436876612755
1114	16	-0.04750966005929058
1114	17	-0.2048760938374059
1114	18	-0.17999389028392443
1114	19	-0.17045930498125728
1114	20	-0.1824626504497644
1114	21	0.0146233062235126
1114	22	-0.10060936458386038
1114	23	-0.1596613042398829
1114	24	-0.17800365636401239
1114	25	-0.17654563674477686
1115	1	-0.690977766867114
1115	2	-0.6537654794417023
1115	3	-0.7604961846948015
1115	4	-0.8082895636872394
1115	5	-0.7395390090836784
1115	6	-0.8381449165820101
1115	7	-0.9727287469518799
1115	8	-0.8234654651411759
1115	9	-0.8934761183898772
1115	10	-0.9832172281114708
1115	11	-0.8201034005440633
1115	12	-0.9569609398852919
1115	13	-0.9729573069047177
1115	14	-0.9425230045436521
1115	15	-1.1186599523534895
1115	16	-1.0813786456641452
1115	17	-1.2189368198035027
1115	18	-0.9537082254425187
1115	19	-1.193166471215875
1115	20	-1.0610415875407284
1115	21	-1.1783665595301756
1115	22	-1.2384961514770438
1115	23	-1.2037828684240282
1115	24	-1.0903491491559194
1115	25	-1.2228210352099609
1116	1	-0.9782139353607633
1116	2	-0.8430117832611401
1116	3	-1.0303692065325158
1116	4	-0.9569551720734637
1116	5	-1.0817519837081804
1116	6	-1.071828376534988
1116	7	-1.0335192350865015
1116	8	-1.1162289469856432
1116	9	-1.28343166043107
1116	10	-1.0414124726656553
1116	11	-1.0904369580593496
1116	12	-1.283950344466433
1116	13	-1.2685977535778505
1116	14	-1.5477527512091953
1116	15	-1.526390047021124
1116	16	-1.4457263237654203
1116	17	-1.334410663656456
1116	18	-1.4907100872813714
1116	19	-1.361411153091062
1116	20	-1.4685278041128882
1116	21	-1.6676579431754277
1116	22	-1.9197678407489036
1116	23	-1.781313419024201
1116	24	-1.927625141481947
1116	25	-1.9814304077859788
1201	1	0.5096281820119621
1201	2	0.4032923021121242
1201	3	0.3988005085021662
1201	4	0.36436151609335354
1201	5	0.5116596635997297
1201	6	0.5117192258320102
1201	7	0.513072064720383
1201	8	0.4908619663355722
1201	9	0.5862355482634314
1201	10	0.4103959958945751
1201	11	0.4553829215225704
1201	12	0.5481695875948134
1201	13	0.6367520353415159
1201	14	0.7118772049201567
1201	15	0.8063402480264301
1201	16	0.6379665661908689
1201	17	0.6443132356349466
1201	18	0.7109746800128889
1201	19	0.7586100971577636
1201	20	0.7216869991930537
1201	21	0.691768817144002
1201	22	0.8631133000430395
1201	23	0.8056512853982838
1201	24	0.7782652103363432
1201	25	0.9916626926046419
1202	1	0.6761551838741582
1202	2	0.6686926745516004
1202	3	0.6175031776517628
1202	4	0.662111360910238
1202	5	0.6941115568337466
1202	6	0.6442207776631719
1202	7	0.6136772788730838
1202	8	0.5530314262983254
1202	9	0.666213822627185
1202	10	0.6575933888182277
1202	11	0.6027261865753388
1202	12	0.5532502828151706
1202	13	0.6159140465147033
1202	14	0.562535181332322
1202	15	0.6661136806769576
1202	16	0.6058467648252517
1202	17	0.6089190705201091
1202	18	0.5117692175672062
1202	19	0.5481973100689999
1202	20	0.5449117043451324
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2210	20	-0.17941551278025258
2210	21	-0.3224886672380265
2210	22	-0.3159213186051275
2210	23	-0.18218235830128293
2210	24	-0.35098267831905616
2210	25	-0.1696844676867073
2211	1	-0.5482105313653669
2211	2	-0.5046106137141315
2211	3	-0.6357205468238405
2211	4	-0.7135257058009401
2211	5	-0.5075403654941866
2211	6	-0.34581316213541413
2211	7	-0.40963685900039337
2211	8	-0.43691265649252387
2211	9	-0.4333485765271021
2211	10	-0.41775880614088123
2211	11	-0.4082575111935398
2211	12	-0.44491668986606925
2211	13	-0.49831229299456226
2211	14	-0.5943239963230482
2211	15	-0.5220232469366834
2211	16	-0.5538630777163362
2211	17	-0.587828272716969
2211	18	-0.4761537780373146
2211	19	-0.6960621346536442
2211	20	-0.6126382316700045
2211	21	-0.5529018200967784
2211	22	-0.5091632712384637
2211	23	-0.5769133436682239
2211	24	-0.5735938631901476
2211	25	-0.5247356479229097
2212	1	-0.38880525133849986
2212	2	-0.40149525818825893
2212	3	-0.3478743363607677
2212	4	-0.4606466344738678
2212	5	-0.38735563736342016
2212	6	-0.45600342279841755
2212	7	-0.49529898225451774
2212	8	-0.522492807133915
2212	9	-0.35971265280967657
2212	10	-0.3746865320522019
2212	11	-0.43489662911869686
2212	12	-0.6045654112171377
2212	13	-0.6218999989848015
2212	14	-0.6820510369334158
2212	15	-0.5661926167183609
2212	16	-0.5886026252176038
2212	17	-0.5114040419098274
2212	18	-0.6988275513084972
2212	19	-0.533100085249167
2212	20	-0.5553339270429376
2212	21	-0.5356878190754628
2212	22	-0.4578494062768286
2212	23	-0.44050744897058536
2212	24	-0.3397953414708752
2212	25	-0.403854771712453
2213	1	-0.38258764986867533
2213	2	-0.5541246882657589
2213	3	-0.5521637620151765
2213	4	-0.44189491809603965
2213	5	-0.4649356722134854
2213	6	-0.5517606583182655
2213	7	-0.5352375450417455
2213	8	-0.752541874274975
2213	9	-0.7058814544680985
2213	10	-0.7554983392735011
2213	11	-0.5591930053438574
2213	12	-0.6800571154922253
2213	13	-0.7772309166302591
2213	14	-0.8890973603002195
2213	15	-0.8582747987115198
2213	16	-0.7399121759336893
2213	17	-0.8200025784015423
2213	18	-0.8971142113162216
2213	19	-0.9024237655520793
2213	20	-0.9165392921058668
2213	21	-1.0332624101218641
2213	22	-1.123222197465644
2213	23	-1.0454365215554446
2213	24	-1.0637728663386028
2213	25	-0.9890697412004172
2214	1	-0.7502774808822856
2214	2	-0.8422146797941465
2214	3	-0.7712997513517408
2214	4	-0.898546073028001
2214	5	-0.8112784463499523
2214	6	-0.9121179679592402
2214	7	-0.8323351375548071
2214	8	-0.7753687647236376
2214	9	-0.8773694311136637
2214	10	-0.8150856942763269
2214	11	-0.9499544883689524
2214	12	-0.9064552249848887
2214	13	-0.9286036230172954
2214	14	-0.9657710905556058
2214	15	-1.0055020467983695
2214	16	-0.9757848811473622
2214	17	-0.9060061125426131
2214	18	-0.9925224093032199
2214	19	-0.8964938710835
2214	20	-0.8863092443973102
2214	21	-1.01532300679564
2214	22	-0.9492321615844901
2214	23	-1.0658181430831988
2214	24	-1.0990410597729863
2214	25	-1.0505264831729164
2215	1	-0.7212499588037957
2215	2	-0.6775098967873738
2215	3	-0.6380116127736599
2215	4	-0.6698291638101566
2215	5	-0.7763504479744512
2215	6	-0.7941632507678946
2215	7	-0.634621560888597
2215	8	-0.8052768208010761
2215	9	-0.7692272769860722
2215	10	-0.8928503239443941
2215	11	-0.6599883529877459
2215	12	-0.88819896982411
2215	13	-0.8118281924316761
2215	14	-0.8705337012850052
2215	15	-0.9049028344270482
2215	16	-0.8201734086878645
2215	17	-1.022685403191609
2215	18	-0.9806275630327761
2215	19	-1.2252870632977846
2215	20	-1.2596722822755453
2215	21	-1.1173032199387762
2215	22	-1.1785780236587229
2215	23	-1.3865306388489955
2215	24	-1.3747690502416532
2215	25	-1.3099223649693172
2216	1	-1.3791562135794386
2216	2	-1.4657359187909336
2216	3	-1.1979956207324491
2216	4	-1.1770216860806073
2216	5	-1.3232535809685757
2216	6	-1.4863677584108714
2216	7	-1.382959475230308
2216	8	-1.3921741267005956
2216	9	-1.6260045646510335
2216	10	-1.4486982203242817
2216	11	-1.3717939340975676
2216	12	-1.5011801001679004
2216	13	-1.4655355623210085
2216	14	-1.6666957845622363
2216	15	-1.6056797948049635
2216	16	-1.6984527187356164
2216	17	-1.7607828264988612
2216	18	-1.9502883577255332
2216	19	-1.9647481825934747
2216	20	-1.8019617936351366
2216	21	-1.8680609370510837
2216	22	-2.1309733448394326
2216	23	-2.117474069708962
2216	24	-2.1217144794573883
2216	25	-2.3264637808494015
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31418.0
3	1.0
4	267.0
5	1848.0
6	3707.0
7	3947.0
8	3232.0
9	2755.0
10	2641.0
11	3633.0
12	3651.0
13	3232.0
14	1693.0
15	1552.0
16	1560.0
17	1831.0
18	2043.0
19	2684.0
20	3523.0
21	4525.0
22	5585.0
23	6650.0
24	7589.0
25	8464.0
26	9380.0
27	10265.0
28	11451.0
29	13447.0
30	16596.0
31	21191.0
32	27515.0
33	36268.0
34	49755.0
35	70586.0
36	105672.0
37	197948.0
38	560520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.39393275167166	14.953536356820074	13.34540657406561	23.30712431744265
2	11.731284636957447	21.35077976810045	40.80262859330045	26.115307001641657
3	20.272769413904328	30.29334664230995	27.474637839164895	21.95924610462083
4	13.282173183649107	14.5924351795088	32.8571901656849	39.26820147115719
5	17.007899833152166	33.599406906822026	30.009836430974584	19.382856829051228
6	36.89373015251111	31.191790460603112	14.391494469967533	17.52298491691824
7	25.92233527390122	32.83785028381411	22.45742788310058	18.78238655918409
8	26.38919186190248	34.21260141516675	22.354076077735606	17.04413064519517
9	29.603543943461048	18.588658767003004	15.698541855319673	36.10925543421627
10	14.48085688277471	26.08085655969094	29.914407032241336	29.52387952529302
11	32.14373919486293	25.970756262105006	17.838875095865127	24.046629447166943
12	26.953328602813308	23.807397472473408	26.351811047658636	22.887462877054652
13	31.73383873023088	20.538945728002858	26.376604560747058	21.35061098101921
14	30.380323148478034	19.7407215938464	23.19668492414719	26.68227033352838
15	33.04686961003543	21.281375934936914	25.366324820317605	20.305429634710055
16	26.067671753773947	22.796472700922074	30.565732745504253	20.570122799799726
17	23.7877243497145	29.383036256277244	26.744157737042173	20.085081656966082
18	26.57152548864944	19.720451782639252	34.958458914170066	18.749563814541244
19	23.642808096319516	32.357925630607454	20.749050781370478	23.250215491702555
20	20.83627188493917	27.652629520128098	29.0010493571166	22.510049237816133
21	22.220544848772608	32.0949447481302	21.556873105345815	24.127637297751374
22	20.874865391903306	28.753263257325234	20.089654875795375	30.28221647497608
23	24.515809635112323	24.84750357096481	20.621185560316842	30.015501233606024
24	19.187308913166248	28.84291445151993	21.61699220398175	30.352784431332076
25	26.046264041137295	27.62688854970256	21.425864661410092	24.90098274775005
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	458.0
1	458.0
2	750.0
3	1042.0
4	1042.0
5	1042.0
6	2328.0
7	3614.0
8	3614.0
9	3614.0
10	3729.5
11	3845.0
12	3845.0
13	3845.0
14	4522.5
15	5200.0
16	5200.0
17	5200.0
18	8141.5
19	11083.0
20	11083.0
21	11083.0
22	13368.0
23	15653.0
24	15653.0
25	15653.0
26	23275.0
27	30897.0
28	30897.0
29	30897.0
30	36629.0
31	42361.0
32	42361.0
33	42361.0
34	48404.5
35	54448.0
36	54448.0
37	54448.0
38	59967.0
39	65486.0
40	65486.0
41	65486.0
42	77132.0
43	88778.0
44	88778.0
45	88778.0
46	153070.5
47	217363.0
48	217363.0
49	217363.0
50	309371.0
51	401379.0
52	401379.0
53	401379.0
54	257977.0
55	114575.0
56	114575.0
57	114575.0
58	88486.5
59	62398.0
60	62398.0
61	62398.0
62	54447.0
63	46496.0
64	46496.0
65	46496.0
66	38650.5
67	30805.0
68	30805.0
69	30805.0
70	24009.5
71	17214.0
72	17214.0
73	17214.0
74	14260.5
75	11307.0
76	11307.0
77	11307.0
78	9538.0
79	7769.0
80	7769.0
81	7769.0
82	5782.5
83	3796.0
84	3796.0
85	3796.0
86	2846.5
87	1897.0
88	1897.0
89	1897.0
90	1282.5
91	668.0
92	668.0
93	668.0
94	371.5
95	75.0
96	75.0
97	75.0
98	46.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1842365526289232
2	0.01969926329599354
3	0.02930669088707236
4	0.04456554647290342
5	0.0299525683721869
6	0.06038954485820971
7	0.06805933999394491
8	0.07193460490463215
9	0.055061055606014736
10	0.0448884852154607
11	0.6239983853062873
12	0.557634473710768
13	0.5320415783631042
14	0.5196084367746493
15	0.5320415783631042
16	0.5081441114138662
17	0.5164597840347159
18	0.5127459884953073
19	0.5295388031082854
20	0.5200928448884853
21	0.5280048440811383
22	0.512180845695832
23	0.5219497426581895
24	0.5006357856494097
25	0.5057220708446867
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1238625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.68993240379645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09910376886373	32.827593334797264
2	8.527891737709353	6.428313262823196
3	1.770194766061634	2.0015556322325176
4	0.6635898225899237	1.00042622229046
5	0.3451428677693554	0.6504205677939731
6	0.235039609745732	0.5315176202118801
7	0.16172902776883258	0.4266889287037313
8	0.12100407213801163	0.36485082395726137
9	0.1046820586938707	0.35509137444560157
>10	0.7094124525652457	5.5373329200233
>50	0.11366770430766597	3.00205002260233
>100	0.11840313258555252	8.786465647316348
>500	0.013777819063329767	3.5699225739502642
>1k	0.012701426949007126	10.864579583096692
>5k	0.0012916705371871656	3.5633503722935984
>10k+	0.0023680626515098033	20.08984111346164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	59753	4.824139671006156	No Hit
CTTCCGTACTCTGCGTTGATACCAC	40286	3.2524775456655566	No Hit
GAGTACGGAAGCAGTGGTATCAACG	28010	2.2613785447572914	No Hit
TCCGTACTCTGCGTTGATACCACTG	27958	2.2571803411040468	No Hit
GCTTCCGTACTCTGCGTTGATACCA	16498	1.331960843677465	No Hit
GCGTTGATACCACTGCTTCCGTACT	15041	1.2143304067009788	No Hit
GTACATGGGAAGCAGTGGTATCAAC	12906	1.0419618528610353	No Hit
GTACATGGAAGCAGTGGTATCAACG	12608	1.0179029165405187	No Hit
GTATCAACGCAGAGTACGGAAGCAG	12184	0.983671409829448	No Hit
CCCATGTACTCTGCGTTGATACCAC	11869	0.9582399838530629	No Hit
TCCATGTACTCTGCGTTGATACCAC	10487	0.8466646482995256	No Hit
TATCAACGCAGAGTACGGAAGCAGT	9161	0.7396104551417904	No Hit
ACTCTGCGTTGATACCACTGCTTCC	8712	0.7033605812897366	No Hit
GGTATCAACGCAGAGTACGGAAGCA	6786	0.5478655767484105	No Hit
ATACCACTGCTTCCGTACTCTGCGT	6764	0.5460894136643455	No Hit
CAGTGGTATCAACGCAGAGTACGGA	6480	0.5231607629427792	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	6014	0.48553839943485716	No Hit
CATGTACTCTGCGTTGATACCACTG	4927	0.39777979614491876	No Hit
GTGGTATCAACGCAGAGTACGGAAG	4678	0.37767685942072865	No Hit
GTACTCTGCGTTGATACCACTGCTT	4517	0.3646785750327985	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	4420	0.3568473105257846	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4386	0.3541023312140478	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4380	0.35361792310021195	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4000	0.3229387425572712	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3947	0.31865980421838735	No Hit
GTTGATACCACTGCTTCCGTACTCT	3921	0.3165607023917651	No Hit
GATACCACTGCTTCCGTACTCTGCG	3619	0.29217882732869105	No Hit
GTATCAACGCAGAGTACATGGGAAG	3536	0.28547784842062773	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	3392	0.27385205368856597	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	3266	0.2636794832980119	No Hit
GCGTTGATACCACTGCTTCCCATGT	3070	0.2478554849127056	No Hit
TATCAACGCAGAGTACATGGGAAGC	2812	0.2270259360177616	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2739	0.2211323039660914	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2682	0.2165304268846503	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2599	0.20982944797658695	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2591	0.2091835704914724	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2534	0.2045816934100313	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2416	0.1950550005045918	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2347	0.18948430719547885	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2343	0.18916136845292159	No Hit
GCGTTGATACCACTGCTTCCATGTA	2342	0.18908063376728226	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	2298	0.1855283075991523	No Hit
CAGTGGTATCAACGCAGAGTACATG	2281	0.1841558179432839	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	2243	0.1810878998889898	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2165	0.17479059440912303	No Hit
TGATACCACTGCTTCCGTACTCTGC	2025	0.16348773841961853	No Hit
CCCGTACTCTGCGTTGATACCACTG	1962	0.15840145322434152	No Hit
GTATCAACGCAGAGTACATGGAAGC	1925	0.15541426985568674	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1883	0.1520234130588354	No Hit
GGTATCAACGCAGAGTACATGGGAA	1869	0.15089312745988495	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1860	0.15016651528913108	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1825	0.14734080129175497	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1780	0.14370774043798568	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1675	0.1352305984458573	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1621	0.13087092542133416	No Hit
TATCAACGCAGAGTACATGGAAGCA	1574	0.12707639519628622	No Hit
AAAGTACTCTGCGTTGATACCACTG	1558	0.12578464022605712	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1367	0.11036431526894742	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1335	0.10778080532848926	No Hit
CCATGTACTCTGCGTTGATACCACT	1334	0.10770007064284993	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1315	0.10616611161570289	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	1254	0.1012412957917045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.073468563931779E-5	0.0
8	0.0	0.0	0.0	8.073468563931779E-5	0.0
9	0.0	0.0	0.0	8.073468563931779E-5	0.0
10	0.0	0.0	0.0	5.651427994752246E-4	0.0
11	0.0	0.0	0.0	6.458774851145423E-4	0.0
12	0.0	0.0	0.0	9.688162276718135E-4	0.0
13	0.0	0.0	0.0	0.0013724896558684025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACT	50	9.0074085E-5	15.140609	4
GTCTATA	40	0.0053679766	14.213852	1
TTTCGTT	65	5.423133E-5	13.160956	17
TTCGTTC	65	5.4251985E-5	13.160422	18
GCCTTAG	65	5.5821536E-5	13.120479	1
AGGCCCG	90	5.3114127E-7	12.684326	10
GTCGTAG	105	1.9534127E-8	12.684326	11
CGTCAAT	90	5.369275E-7	12.672999	19
CGGAATT	60	4.0737446E-4	12.672998	15
CGTCACT	60	4.0737446E-4	12.672998	15
CGACTTT	70	1.0850595E-4	12.220887	13
TCGTTCT	70	1.0854707E-4	12.220391	19
CGTCGTA	110	3.7372956E-8	12.107766	10
TATTTGA	55	0.0031591016	12.043668	2
TCCGTCA	95	1.0324202E-6	12.006486	17
CCGTCAA	95	1.0329277E-6	12.005999	18
CCGTCGT	120	9.798896E-9	11.891556	9
TCGGAAT	65	7.9877884E-4	11.698153	14
CTTTCGT	90	7.4189174E-6	11.616445	16
CCTATTC	140	7.239578E-10	11.490641	3
>>END_MODULE