FastQCFastQC Report
Thu 2 Feb 2017
SRR4050256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238625
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA528584.26747401352306No Hit
CTTCCGTACTCTGCGTTGATACCAC386543.12071853870219No Hit
TCCGTACTCTGCGTTGATACCACTG287022.317246947219699No Hit
GAGTACGGAAGCAGTGGTATCAACG286452.312645070138258No Hit
GCTTCCGTACTCTGCGTTGATACCA161291.3021697446765566No Hit
GCGTTGATACCACTGCTTCCGTACT143661.1598344938944394No Hit
CCCATGTACTCTGCGTTGATACCAC125701.0148349984862246No Hit
GTATCAACGCAGAGTACGGAAGCAG119280.9630033303057827No Hit
TCCATGTACTCTGCGTTGATACCAC115790.9348269250176608No Hit
GTACATGGGAAGCAGTGGTATCAAC112010.9043092138459986No Hit
GTACATGGAAGCAGTGGTATCAACG110980.8959935412251488No Hit
TATCAACGCAGAGTACGGAAGCAGT108670.8773438288424664No Hit
ACTCTGCGTTGATACCACTGCTTCC94780.765203350489454No Hit
CAGTGGTATCAACGCAGAGTACGGA76450.6172166717125845No Hit
ATACCACTGCTTCCGTACTCTGCGT70690.5707134927843375No Hit
ACGCAGAGTACGGAAGCAGTGGTAT70690.5707134927843375No Hit
GGTATCAACGCAGAGTACGGAAGCA70600.5699868806135836No Hit
GTATCAACGCAGAGTACTTTTTTTT68130.5500454132606721No Hit
CATGTACTCTGCGTTGATACCACTG54260.43806640427893834No Hit
GTGGTATCAACGCAGAGTACGGAAG53150.42910485417297406No Hit
AAGCAGTGGTATCAACGCAGAGTAC53100.4287011807447775No Hit
GAAGCAGTGGTATCAACGCAGAGTA51570.41634877384196184No Hit
TATCAACGCAGAGTACTTTTTTTTT49810.4021394691694419No Hit
ATCAACGCAGAGTACGGAAGCAGTG39600.31970935513169846No Hit
GTTGATACCACTGCTTCCGTACTCT39220.3166414370774044No Hit
GAGTACATGGGAAGCAGTGGTATCA39020.315026743364618No Hit
GAGTACATGGAAGCAGTGGTATCAA38310.30929458068422644No Hit
GATACCACTGCTTCCGTACTCTGCG38100.3075991522858008No Hit
GTACTCTGCGTTGATACCACTGCTT37730.30461196891714604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36740.29661923503885357No Hit
CCACTGCTTCCGTACTCTGCGTTGA35320.28515490967807045No Hit
GTATCAACGCAGAGTACATGGGAAG34220.2762740942577455No Hit
CGGAAGCAGTGGTATCAACGCAGAG31190.2518114845090322No Hit
TATCAACGCAGAGTACATGGGAAGC30600.24704813805631246No Hit
GCGTTGATACCACTGCTTCCCATGT30310.24470683217277223No Hit
CATGGAAGCAGTGGTATCAACGCAG27660.22331214047835302No Hit
AAAAAGTACTCTGCGTTGATACCAC27590.22274699767887782No Hit
CTGCTTCCGTACTCTGCGTTGATAC27490.22193965082248462No Hit
GCAGAGTACGGAAGCAGTGGTATCA26960.21766071248360075No Hit
GGTATCAACGCAGAGTACTTTTTTT26670.21531940660006055No Hit
CAGTGGTATCAACGCAGAGTACATG25710.20756887677868605No Hit
ACGCAGAGTACATGGGAAGCAGTGG24810.20030275507114745No Hit
GCGTTGATACCACTGCTTCCATGTA24200.19537793924714908No Hit
GTACGGGAAGCAGTGGTATCAACGC24030.19400544959128066No Hit
CACTGCTTCCGTACTCTGCGTTGAT23020.18585124634170955No Hit
GCTTCCATGTACTCTGCGTTGATAC22970.18544757291351296No Hit
TGATACCACTGCTTCCGTACTCTGC20540.16582904430315876No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20480.16534463618932282No Hit
CCCGTACTCTGCGTTGATACCACTG20370.16445655464729034No Hit
GCAGTGGTATCAACGCAGAGTACGG19520.15759410636794832No Hit
TACCACTGCTTCCGTACTCTGCGTT19490.15735190231103038No Hit
GCTTCCCATGTACTCTGCGTTGATA19140.15452618831365425No Hit
TTCCCGTACTCTGCGTTGATACCAC19010.15347663740034312No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18400.14855182157634475No Hit
GTATCAACGCAGAGTACATGGAAGC18360.14822888283378746No Hit
TATCAACGCAGAGTACATGGAAGCA17930.1447572913512968No Hit
CTGCGTTGATACCACTGCTTCCGTA16900.13644161873044708No Hit
TCAACGCAGAGTACGGAAGCAGTGG16850.13603794530225047No Hit
AAAGTACTCTGCGTTGATACCACTG16820.13579574124533253No Hit
GGTATCAACGCAGAGTACATGGGAA16460.1328892925623171No Hit
ACGCAGAGTACATGGAAGCAGTGGT15840.12788374205267938No Hit
CCATGTACTCTGCGTTGATACCACT14840.1198102734887476No Hit
GAGTACGGGAAGCAGTGGTATCAAC14520.11722676354828944No Hit
GTATCAACGCAGAGTACGGGAAGCA13740.11092945806842264No Hit
TACGGAAGCAGTGGTATCAACGCAG13040.10527803007367041No Hit
GGGAAGCAGTGGTATCAACGCAGAG12480.1007568876778686No Hit
ATACCACTGCTTCCATGTACTCTGC12470.10067615299222928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG400.00522609214.2713652
CAGACCG400.00527539514.2511835
GTGTAGG551.9045512E-413.8647061
GCTCTTA1009.600626E-913.3447791
CGGAAGT500.001482866813.3194014
AGATAGT500.001501963913.2968046
CGGACCA1001.0137228E-813.2930469
TCTTACA655.3746495E-513.1735672
TTACCGC1900.012.98953116
GTTTATA751.4277923E-512.7093141
AGACTGT604.0992803E-412.6636236
ATTACCG1950.012.65646715
GATCCAT1155.3641998E-912.38432612
ACGGTAT853.9645947E-612.2876899
CGAGGTT550.003034524412.1085464
TACCGCG2050.012.03907817
ATCGCCA951.049355E-611.99033616
TCGCCAG1052.7465103E-711.75243417
GTATAGA657.798335E-411.7316731
GCTCCAT657.798335E-411.7316731
Produced by FastQC (version 0.11.5)