FastQCFastQC Report
Thu 2 Feb 2017
SRR4050255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5342520
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA111360.20844096044563243No Hit
GTATCTGATCGTCTTCGAACCTCCG73120.13686425132708907No Hit
GAATAGGACCGCGGTTCTATTTTGT69380.1298638095879847No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG64900.12147825370798798No Hit
GTCCTATTCCATTATTCCTAGCTGC64230.12022416387772061No Hit
GATTAAGAGGGACGGCCGGGGGCAT61020.1142157633476337No Hit
CTATTGGAGCTGGAATTACCGCGGC59680.11170758368709897No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC59250.11090272006468857No Hit
GGGTAGGCACACGCTGAGCCAGTCA58510.10951760592379627No Hit
GACTTAATCAACGCAAGCTTATGAC57690.10798274971361829No Hit
GTCTTGCGCCGGTCCAAGAATTTCA57540.10770198333370769No Hit
CTTATGACCCGCACTTACTGGGAAT57500.10762711229906487No Hit
GAACTACGACGGTATCTGATCGTCT57240.10714045057388648No Hit
ATACAGGACTCTTTCGAGGCCCTGT53640.10040205745603199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.016.00271
ACGCTTA2500.014.07105217
CTCGACG2600.013.53049613
GTATCAA85150.013.4012331
CGCTTAC2700.013.3808818
TCGACGC2800.012.56403114
AACCGCG6300.012.0738847
CGTCGTA9400.011.63355810
GACGCTT2950.011.602880516
ACCGTTA752.0605233E-411.40896118
CGGTCCA10400.011.33785510
GCTCGTA5400.011.2701269
AGAACCG7600.011.0094775
CTCGTAG5550.010.965528510
CGAACGA4650.010.83697916
CCGTCCA2550.010.8143499
CCGTCGT10300.010.8016789
CCGACCA10400.010.7892499
TCCATTA12750.010.7397678
CGACGCT3550.010.71314415
Produced by FastQC (version 0.11.5)