FastQCFastQC Report
Thu 2 Feb 2017
SRR4050255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5342520
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA104190.19502032748590553No Hit
GAATAGGACCGCGGTTCTATTTTGT70900.13270890890441214No Hit
GTATCAACGCAGAGTACTTTTTTTT68380.127992033721914No Hit
GATTAAGAGGGACGGCCGGGGGCAT64740.12117876956941667No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG59670.11168886592843827No Hit
CTTATGACCCGCACTTACTGGGAAT59260.11092143782334928No Hit
ATCAGATACCGTCGTAGTTCCGACC58540.10957375919977838No Hit
ATACAGGACTCTTTCGAGGCCCTGT58500.10949888816513555No Hit
GTATCTGATCGTCTTCGAACCTCCG57440.10751480574710062No Hit
TCGTAGTTCCGACCATAAACGATGC54450.10191819590754925No Hit
GAACTACGACGGTATCTGATCGTCT54230.10150640521701369No Hit
CTATTGGAGCTGGAATTACCGCGGC54140.10133794538906733No Hit
GTCCTATTCCATTATTCCTAGCTGC54020.10111333228513884No Hit
GACTTAATCAACGCAAGCTTATGAC53640.10040205745603199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.016.5218241
ACGCTTA2450.013.56164717
TTCGCGG500.00150855513.29041517
GTATCAA82400.012.9601981
CTCGACG2600.012.77948513
CGACCGT1457.2759576E-1212.45235719
GGTAGCG2900.011.78736211
GTTCGAC1459.822543E-1111.78460416
TCGACGC2850.011.65825914
AACCGCG6900.011.4232577
CGTCGTA10150.011.4131610
ACCGTTA1251.8551873E-811.39178518
CGGTAGC2850.011.32781710
CGAACGA5650.011.25736816
GCGCGCT4550.011.0579615
TACCGTC10750.011.0423757
GCGTTAT5200.010.9823161
ATACCGT10900.010.9804176
CGACCAT9100.010.64316810
CGCTTAC2950.010.61946118
Produced by FastQC (version 0.11.5)