##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5342520
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.165099615911593	31.0	30.0	34.0	26.0	34.0
2	30.296520930197733	31.0	30.0	34.0	26.0	34.0
3	30.41313350254187	31.0	30.0	34.0	26.0	34.0
4	33.97298596916811	37.0	35.0	37.0	30.0	37.0
5	33.78661773844553	35.0	35.0	37.0	29.0	37.0
6	33.844817239804435	36.0	35.0	37.0	30.0	37.0
7	33.779101809632905	37.0	35.0	37.0	29.0	37.0
8	33.70871423972208	37.0	35.0	37.0	29.0	37.0
9	35.278907332120426	38.0	35.0	39.0	30.0	39.0
10	35.10919771942829	38.0	35.0	39.0	29.0	39.0
11	35.095903618517106	38.0	35.0	39.0	29.0	39.0
12	34.974062053113514	38.0	35.0	39.0	29.0	39.0
13	34.99103475513428	38.0	35.0	39.0	29.0	39.0
14	36.04763332659494	39.0	36.0	41.0	29.0	41.0
15	36.00423639031768	39.0	36.0	41.0	29.0	41.0
16	35.95412801449503	39.0	36.0	41.0	28.0	41.0
17	35.91917540785996	39.0	36.0	41.0	28.0	41.0
18	35.859677642760346	39.0	36.0	41.0	28.0	41.0
19	35.85509029446778	39.0	36.0	41.0	27.0	41.0
20	35.805341673966595	39.0	36.0	41.0	27.0	41.0
21	35.743512237670615	39.0	36.0	41.0	27.0	41.0
22	35.66617438961389	39.0	36.0	41.0	27.0	41.0
23	35.571813114410425	39.0	36.0	41.0	27.0	41.0
24	35.46736895697162	39.0	36.0	41.0	27.0	41.0
25	35.125427700785394	39.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8787997803873324
1101	2	0.8620092867724161
1101	3	0.8832671778834573
1101	4	0.8398447764250037
1101	5	0.9059286118792471
1101	6	0.9699430483777718
1101	7	1.0353530481289965
1101	8	0.9820481050806862
1101	9	1.1182601790033004
1101	10	1.1624940332946707
1101	11	1.1161203963152033
1101	12	1.081842607437764
1101	13	1.085590811599502
1101	14	1.2044426162501694
1101	15	1.2118643873609756
1101	16	1.3439491642962764
1101	17	1.2688418263306431
1101	18	1.4149349422736464
1101	19	1.333381409651352
1101	20	1.4389437458498193
1101	21	1.3648917248410726
1101	22	1.3750290540035621
1101	23	1.368942385928328
1101	24	1.4481187141935976
1101	25	1.5775897979449667
1102	1	0.8450304411406719
1102	2	0.8067652758478872
1102	3	0.8119024399334087
1102	4	0.8308911475901013
1102	5	0.8441600019385262
1102	6	0.8440232677289004
1102	7	0.8898134316492659
1102	8	0.9954527053016307
1102	9	1.1286049750611724
1102	10	1.08157657072001
1102	11	1.0928131552107487
1102	12	1.1218518151942263
1102	13	1.1054156706057299
1102	14	1.1284594247083959
1102	15	1.2703994720325298
1102	16	1.070797465432939
1102	17	1.101808068501093
1102	18	1.1988723553458627
1102	19	1.3021182797240982
1102	20	1.2817175932398612
1102	21	1.148290172215411
1102	22	1.2897969077465277
1102	23	1.3019422428638876
1102	24	1.3399375795527533
1102	25	1.4994870475579916
1103	1	0.8255950942433365
1103	2	0.7640839849237899
1103	3	0.7424030864736828
1103	4	0.753267202264702
1103	5	0.8037059121121075
1103	6	0.7699944457482815
1103	7	0.8624319554444
1103	8	0.8650639909640816
1103	9	0.9816161376259487
1103	10	1.0231243350225796
1103	11	1.0123710051716444
1103	12	0.9805699608960978
1103	13	1.0765861186014547
1103	14	1.2267417919575436
1103	15	1.2020138908981366
1103	16	1.1310616127463362
1103	17	1.1166864758529158
1103	18	1.1863612394250111
1103	19	1.1587435403288495
1103	20	1.1346511260453411
1103	21	1.2122837060198322
1103	22	1.2765311512838338
1103	23	1.2960246749652669
1103	24	1.3405260858029067
1103	25	1.385818805568789
1104	1	0.724775936052847
1104	2	0.6650348973636824
1104	3	0.6765381715720302
1104	4	0.6782265904076326
1104	5	0.7939849652041175
1104	6	0.8200544171446467
1104	7	0.858510780198884
1104	8	0.7521772557748747
1104	9	0.9163056721965575
1104	10	0.895885090509978
1104	11	0.8768088033848187
1104	12	0.8609130798843836
1104	13	0.9537240678385572
1104	14	0.9808056213911343
1104	15	1.0320176793096536
1104	16	1.01824133727483
1104	17	1.06736655326538
1104	18	1.148356842833124
1104	19	1.0914563978487521
1104	20	1.1225976791974617
1104	21	1.0720710756657823
1104	22	1.0802984545066536
1104	23	1.161898714129606
1104	24	1.1951074874803922
1104	25	1.3740686314623645
1105	1	0.7526155839855448
1105	2	0.6549918467566442
1105	3	0.6622693996319846
1105	4	0.7049843845720716
1105	5	0.7395950112447309
1105	6	0.7798137247345878
1105	7	0.7614802078418137
1105	8	0.8134471878154059
1105	9	0.856792158543378
1105	10	0.9537471703257694
1105	11	0.9813868611973788
1105	12	0.9637056162091753
1105	13	1.0049124493666781
1105	14	1.0905912573888088
1105	15	1.0825091289948574
1105	16	1.0585459048200505
1105	17	1.0465639038960006
1105	18	1.0453579752959499
1105	19	1.0526370396746998
1105	20	1.15100904108386
1105	21	1.1594582251480858
1105	22	1.1392490339842212
1105	23	1.1854841160915655
1105	24	1.2337884388160134
1105	25	1.2554321821609378
1106	1	0.6660123853173019
1106	2	0.6430162049858907
1106	3	0.5677289103131038
1106	4	0.6006376031297478
1106	5	0.6087470434296876
1106	6	0.6154715927426224
1106	7	0.6860142677884369
1106	8	0.6939296384777336
1106	9	0.7658723635142479
1106	10	0.7724689382748338
1106	11	0.7845840601944332
1106	12	0.8788049855923106
1106	13	0.8082761074713076
1106	14	0.8513931800794765
1106	15	0.8776662927335579
1106	16	0.7588220964529739
1106	17	0.8295733414890947
1106	18	0.8013293088825577
1106	19	0.9223499932024026
1106	20	0.9628000687748539
1106	21	0.9239310713986058
1106	22	0.9454862019051404
1106	23	1.0072807604449707
1106	24	1.088482211273977
1106	25	1.146135191205019
1107	1	0.43802540146655744
1107	2	0.42508594039681924
1107	3	0.41207100942679276
1107	4	0.40365800434307886
1107	5	0.5359899778018118
1107	6	0.4592151531440649
1107	7	0.40075013188670283
1107	8	0.5359276322094644
1107	9	0.5568261682928366
1107	10	0.6019729950621908
1107	11	0.5730431359961514
1107	12	0.570212814939481
1107	13	0.5082567413970622
1107	14	0.6340670537837738
1107	15	0.4527619347991845
1107	16	0.47437177490280646
1107	17	0.48358918447642907
1107	18	0.5703551411501593
1107	19	0.49283656353820504
1107	20	0.5096985320712761
1107	21	0.3512732121110176
1107	22	0.1675904918688147
1107	23	0.47740113230288017
1107	24	0.5667438860442147
1107	25	0.6901901461106021
1108	1	0.42628760273977306
1108	2	0.41335332029915506
1108	3	0.4159874630646563
1108	4	0.4334144329061189
1108	5	0.567427717528119
1108	6	0.5119314697379664
1108	7	0.48839166164143677
1108	8	0.465573443203553
1108	9	0.5721617555091356
1108	10	0.5826803840071335
1108	11	0.516342735944491
1108	12	0.5058884345329702
1108	13	0.49348649640534603
1108	14	0.5266263686993398
1108	15	0.5182019252294907
1108	16	0.5290489332669779
1108	17	0.5745180541596113
1108	18	0.4920460522612444
1108	19	0.48620770035138605
1108	20	0.6326279584827219
1108	21	0.6226732432135549
1108	22	0.6558907509387097
1108	23	0.5211349628666468
1108	24	0.5447296490185636
1108	25	0.7544223751192192
1109	1	0.18984738262068745
1109	2	0.2742635729353182
1109	3	0.15559085169845233
1109	4	0.20708011041293872
1109	5	0.26936915520484206
1109	6	0.31104912110930627
1109	7	0.2722083523885672
1109	8	0.17455235278170989
1109	9	0.3157668991403284
1109	10	0.17026755583333397
1109	11	0.3079723232155729
1109	12	0.22376724496956513
1109	13	0.29001958701667974
1109	14	0.2868165761296524
1109	15	0.33099496524365435
1109	16	0.22871400622456406
1109	17	0.2293195962113117
1109	18	0.13687084013326967
1109	19	0.2343788261325912
1109	20	0.2554388642801939
1109	21	0.2998270344478158
1109	22	0.2830307819105826
1109	23	0.292659340524942
1109	24	0.37747055794029194
1109	25	0.4926655279788221
1110	1	0.31888777598755524
1110	2	0.26982121196563114
1110	3	0.26084269384449854
1110	4	0.37381673572177476
1110	5	0.3879969821449407
1110	6	0.47226585329015336
1110	7	0.5011036027065288
1110	8	0.4811786222390353
1110	9	0.5390723489211169
1110	10	0.5460796434878503
1110	11	0.5390107517974272
1110	12	0.6149651618363592
1110	13	0.6548830575135227
1110	14	0.6401836976171538
1110	15	0.651223182579109
1110	16	0.7240223711622491
1110	17	0.6805583457678281
1110	18	0.6848459204089536
1110	19	0.6024059847383825
1110	20	0.6091013695475809
1110	21	0.6518509295124133
1110	22	0.6388913872026905
1110	23	0.6753705645193975
1110	24	0.6486297939027708
1110	25	0.855014637229317
1111	1	0.22532766110058589
1111	2	0.26377721629307516
1111	3	0.2567949487442043
1111	4	0.322834927863326
1111	5	0.25702512331370997
1111	6	0.3236548295633881
1111	7	0.39996542376214705
1111	8	0.3609852683938257
1111	9	0.3544299384431824
1111	10	0.4057777966517122
1111	11	0.3982105584435871
1111	12	0.4158282207658033
1111	13	0.4287725133349838
1111	14	0.41115445668445005
1111	15	0.4334745783841498
1111	16	0.40183980011877196
1111	17	0.3836011112393294
1111	18	0.4024083831640368
1111	19	0.44216743826824256
1111	20	0.45162000798868007
1111	21	0.46547956225008846
1111	22	0.4225942923697943
1111	23	0.517364769019224
1111	24	0.49232791637555096
1111	25	0.6327622695398745
1112	1	-0.08511366824316724
1112	2	0.029245935293939596
1112	3	-0.007984177070312626
1112	4	0.007441819079843981
1112	5	0.04354970234836486
1112	6	0.1137818843796623
1112	7	0.024684703842510203
1112	8	0.023239811879122385
1112	9	0.12924731413237112
1112	10	0.0752099056465596
1112	11	0.018833126837421332
1112	12	0.09059672367423133
1112	13	0.08999210078776088
1112	14	0.11530201788944794
1112	15	0.07479531509370219
1112	16	0.11312621571984494
1112	17	0.10111139362145849
1112	18	0.139988936512637
1112	19	0.12793803085611444
1112	20	0.09374757799222522
1112	21	0.08838611207782776
1112	22	0.15324156689634805
1112	23	0.10400863182003661
1112	24	0.16382952561507125
1112	25	0.2293280687035235
1113	1	-0.24450453704258024
1113	2	-0.23454939946757136
1113	3	-0.28906967182037846
1113	4	-0.14297193764109295
1113	5	-0.10359997254045084
1113	6	-0.12558396603376565
1113	7	-0.12585582985239796
1113	8	-0.18875195298632264
1113	9	-0.2300189491141893
1113	10	-0.21158063266627636
1113	11	-0.17394254444333512
1113	12	-0.17660218745773903
1113	13	-0.2280902903716182
1113	14	-0.1417725558615146
1113	15	-0.14438119590244725
1113	16	-0.16090828659375234
1113	17	-0.17950474725608956
1113	18	-0.20031157116137166
1113	19	-0.06318329393721456
1113	20	-0.14394172002985073
1113	21	-0.2039962343056203
1113	22	-0.1803537677535374
1113	23	-0.11823857961145023
1113	24	-0.022236441095628834
1113	25	-0.03361307629560173
1114	1	-0.5608293026391209
1114	2	-0.5310388750924169
1114	3	-0.5963919101049804
1114	4	-0.5013711400245313
1114	5	-0.6279002431822178
1114	6	-0.5294993913730934
1114	7	-0.5911579779009699
1114	8	-0.6495416700835577
1114	9	-0.657058897618235
1114	10	-0.7713918158646678
1114	11	-0.7483012532128654
1114	12	-0.7505817229385698
1114	13	-0.6341954779149006
1114	14	-0.8689885458384552
1114	15	-0.9337696800988198
1114	16	-0.9281784195078728
1114	17	-0.7823147218843047
1114	18	-0.7210151862665342
1114	19	-0.8698239911848162
1114	20	-0.8840301555719847
1114	21	-0.7812240085674276
1114	22	-0.7816847692335855
1114	23	-0.8729876578484479
1114	24	-0.8768500518895905
1114	25	-0.798333848656064
1115	1	-1.3190353691775023
1115	2	-1.2119144701302886
1115	3	-1.3034723765233842
1115	4	-1.2332820079446307
1115	5	-1.3717613471229768
1115	6	-1.3064325380940645
1115	7	-1.4199924650321165
1115	8	-1.370591982163205
1115	9	-1.5644840266941316
1115	10	-1.557444369824168
1115	11	-1.547526433455019
1115	12	-1.5446202786693277
1115	13	-1.5713127617921216
1115	14	-1.832445328953746
1115	15	-1.7708837804682958
1115	16	-1.8078051973339413
1115	17	-1.839885774516894
1115	18	-1.898025887838763
1115	19	-1.8322091212119318
1115	20	-1.8637615672428822
1115	21	-1.866410264865884
1115	22	-1.9009293803796226
1115	23	-1.8531584319432852
1115	24	-1.991812845122297
1115	25	-1.8382207075580155
1116	1	-3.6413284784024285
1116	2	-3.618437777956494
1116	3	-3.376235199727038
1116	4	-3.7186844094912317
1116	5	-4.351352918603265
1116	6	-4.217262700687549
1116	7	-4.051331979061679
1116	8	-4.216111803780201
1116	9	-4.762725349200732
1116	10	-4.671148368422369
1116	11	-4.955991538628588
1116	12	-4.522605459632565
1116	13	-4.659142175346457
1116	14	-5.1563528919385035
1116	15	-5.042335375979452
1116	16	-5.055456747787211
1116	17	-5.0146441660660415
1116	18	-5.0888747812248845
1116	19	-5.154262018010126
1116	20	-5.113431937064114
1116	21	-5.0926324839858275
1116	22	-5.228136975906182
1116	23	-5.2901926894125
1116	24	-5.391323538667084
1116	25	-5.260926823299005
1201	1	0.90004132833716
1201	2	0.8571157304066759
1201	3	0.8566422160280993
1201	4	0.7801383697308211
1201	5	0.8398289620069264
1201	6	0.8053589454595027
1201	7	0.8777213085725961
1201	8	0.8903132464969374
1201	9	0.9896896395175645
1201	10	1.0066380273200153
1201	11	1.0062638201784964
1201	12	1.0628074260298632
1201	13	1.045171541728152
1201	14	1.1722043688158976
1201	15	1.176121851665613
1201	16	1.1569902159641998
1201	17	1.1931889506259665
1201	18	1.1992109167689335
1201	19	1.2894076611972096
1201	20	1.205637046847201
1201	21	1.2897136495796815
1201	22	1.2519744308726501
1201	23	1.2570019076346384
1201	24	1.2359642243681819
1201	25	1.2424599856969252
1202	1	0.7280204159464638
1202	2	0.6556147404710231
1202	3	0.7405968027299643
1202	4	0.6735913890345628
1202	5	0.5959566637063176
1202	6	0.6585941789193299
1202	7	0.7455123084076121
1202	8	0.8371897932713921
1202	9	0.8450218866715034
1202	10	0.9480653907913918
1202	11	0.9646964220593688
1202	12	1.0000081767310718
1202	13	0.9862171612628785
1202	14	1.090012280051667
1202	15	1.0277383340942592
1202	16	1.0647183136378473
1202	17	1.13989514597327
1202	18	1.0157152064900998
1202	19	0.8186771711790612
1202	20	1.0747729155157373
1202	21	1.0449120491837078
1202	22	0.9747837857645791
1202	23	1.0480413400715278
1202	24	1.046079716861172
1202	25	1.0725544420572604
1203	1	0.656576555836704
1203	2	0.6284567178389935
1203	3	0.6537355680590977
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2211	11	0.04324444647311765
2211	12	0.0360296993780338
2211	13	0.030151469004607634
2211	14	0.0057242138775563944
2211	15	0.04500704594777716
2211	16	0.07813543538536294
2211	17	0.11862868275451177
2211	18	0.08011128744095686
2211	19	0.11818688849355397
2211	20	0.12370963250899081
2211	21	0.09899051047506191
2211	22	0.11241669614042138
2211	23	0.1536223712311866
2211	24	0.15289830022127404
2211	25	0.03205050694643319
2212	1	-0.06475537752377392
2212	2	-0.0020453438557268555
2212	3	-0.028319575769746308
2212	4	0.004955897482410876
2212	5	0.06452824033345195
2212	6	-0.030555159813339117
2212	7	0.003325108581520908
2212	8	0.007625822735199961
2212	9	-0.06059439880589679
2212	10	0.05977352569396999
2212	11	-0.057287278813220155
2212	12	5.00980140543561E-4
2212	13	0.01309603508619972
2212	14	0.012063788630953809
2212	15	0.07207069675995115
2212	16	0.0061499978473094075
2212	17	-0.016385490724033502
2212	18	-0.005192140250578348
2212	19	0.13287581426659756
2212	20	-0.099398806711541
2212	21	-0.09238049588661568
2212	22	-0.0724917473442872
2212	23	-0.055537895729926845
2212	24	-0.12358879002955803
2212	25	-0.14604284209558216
2213	1	-0.2806234857144716
2213	2	-0.30812823604532724
2213	3	-0.20369572922302837
2213	4	-0.18222974110312862
2213	5	-0.10784924116535421
2213	6	-0.26464374995052964
2213	7	-0.24277969871364036
2213	8	-0.23616714891478807
2213	9	-0.32659281993726097
2213	10	-0.4094114291398654
2213	11	-0.39128912963141005
2213	12	-0.28449135503668543
2213	13	-0.30799255532797787
2213	14	-0.2420670055597114
2213	15	-0.30023406593043944
2213	16	-0.2952124291534304
2213	17	-0.2600334100190693
2213	18	-0.3191151493664037
2213	19	-0.32406224471407796
2213	20	-0.3090726090072806
2213	21	-0.30586470179906655
2213	22	-0.3454969992190584
2213	23	-0.3429856836179326
2213	24	-0.38129787385239666
2213	25	-0.34063488842208756
2214	1	-0.5645539622896791
2214	2	-0.5754513829480423
2214	3	-0.548013990072004
2214	4	-0.4923299037249649
2214	5	-0.49276691663490624
2214	6	-0.5360526040362785
2214	7	-0.6437314951744284
2214	8	-0.695358908027309
2214	9	-0.6127475089655405
2214	10	-0.7396630490693994
2214	11	-0.6169613319513232
2214	12	-0.7497296426391031
2214	13	-0.6641395571820752
2214	14	-0.7940072216884246
2214	15	-0.722689048980186
2214	16	-0.6507697603796387
2214	17	-0.7812245594846701
2214	18	-0.7819766278193754
2214	19	-0.8265049016500683
2214	20	-0.7797583212213794
2214	21	-0.8731526312339142
2214	22	-0.8462753348533596
2214	23	-0.8725757552514466
2214	24	-0.8891812966351367
2214	25	-1.6956371376049049
2215	1	-1.1313709223937707
2215	2	-1.1520207055015206
2215	3	-1.151484135822784
2215	4	-1.016111834259604
2215	5	-1.1726652406876
2215	6	-1.218432726138687
2215	7	-1.191587798451927
2215	8	-1.250218213949168
2215	9	-1.3941188147375954
2215	10	-1.3771799786286678
2215	11	-1.4549173139190898
2215	12	-1.509223868451798
2215	13	-1.5594383948293213
2215	14	-1.776309050198634
2215	15	-1.6156613084998313
2215	16	-1.55868132895624
2215	17	-1.6904220366780507
2215	18	-1.6613533707687367
2215	19	-1.653332391843911
2215	20	-1.6436026082167388
2215	21	-1.753419613840478
2215	22	-1.7847533071730268
2215	23	-1.7110201071809144
2215	24	-1.7448597414756861
2215	25	-1.8714597334338734
2216	1	-2.6865352012006163
2216	2	-2.2499432787455333
2216	3	-2.200817646641802
2216	4	-2.292109830477628
2216	5	-2.5577998034038067
2216	6	-2.5414159836822954
2216	7	-2.556765038229493
2216	8	-2.648716526019072
2216	9	-3.1253264134024086
2216	10	-2.8901081125345485
2216	11	-3.009900769367089
2216	12	-2.904534506195759
2216	13	-3.086644996904159
2216	14	-3.250518946851507
2216	15	-3.3449187242945086
2216	16	-3.4357584047657
2216	17	-3.3516024519572554
2216	18	-3.299619235490681
2216	19	-3.1065408628194575
2216	20	-3.345143935925499
2216	21	-3.3273052457165164
2216	22	-3.3886068276870063
2216	23	-3.578174476632089
2216	24	-3.6190385388668425
2216	25	-3.678716807018869
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108841.0
3	20.0
4	2025.0
5	15303.0
6	16943.0
7	12873.0
8	10370.0
9	8958.0
10	8275.0
11	7632.0
12	7334.0
13	6930.0
14	7040.0
15	7373.0
16	7393.0
17	8157.0
18	8833.0
19	10302.0
20	12650.0
21	16454.0
22	20671.0
23	25562.0
24	31152.0
25	36847.0
26	42792.0
27	50411.0
28	60199.0
29	74594.0
30	95949.0
31	126287.0
32	165985.0
33	221409.0
34	305228.0
35	425763.0
36	627097.0
37	1055424.0
38	1693444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24617623291821	18.653647547644027	13.052221016470863	26.0479552029669
2	17.51744807879273	20.614077285545772	36.09933846483861	25.769136170822897
3	19.16929947021965	24.155715214703115	28.051422125732888	28.623563189344342
4	13.761361090777187	16.859694688113045	35.12143959794135	34.25750462316842
5	15.64155836181359	35.516141641481234	32.89967710726449	15.942622889440688
6	33.49229889221071	36.69877443809839	15.67199877432572	14.136927895365176
7	29.58636698725421	30.294183679564508	20.868131059466826	19.251318273714464
8	26.89810502132656	33.165908355698264	19.276724096616505	20.659262526358667
9	27.19748421361267	14.630872081114491	18.25467319379127	39.91697051148157
10	16.354522914296883	26.297281166761145	30.41377830729473	26.934417611647245
11	37.482952541222836	21.42621230742269	21.780511112958465	19.310324038396008
12	25.017762539697756	23.685969905811266	28.14205987796597	23.15420767652501
13	30.173884493555374	19.71646199133629	24.246717039647102	25.862936475461236
14	23.77647457411383	20.12123787293702	24.492053182189995	31.610234370759155
15	25.276859965124366	27.14768160898111	22.03813558370264	25.537322842191877
16	25.580256582368378	25.994248254241835	23.38587797419052	25.039617189199266
17	25.209744592836618	25.360592986363933	24.1492760811987	25.280386339600746
18	26.50441681942206	24.048319684397647	25.27456195405115	24.17270154212914
19	27.33872779137935	24.309370858695896	24.074743753883933	24.27715759604082
20	26.37541610075965	24.456673110644243	24.171841565003064	24.996069223593047
21	26.763949188724784	24.36914075112564	23.828758552853852	25.03815150729572
22	25.89881290080578	24.451967321033393	24.62858812481528	25.02063165334555
23	24.829003595688167	23.760030397696962	24.97552648474219	26.435439521872677
24	25.275564493546828	25.149799119810844	24.54412625727938	25.03051012936295
25	25.204242464105665	24.38697278160087	24.811376600860463	25.597408153432998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	402.0
1	402.0
2	619.5
3	837.0
4	837.0
5	837.0
6	2147.5
7	3458.0
8	3458.0
9	3458.0
10	5517.5
11	7577.0
12	7577.0
13	7577.0
14	12294.0
15	17011.0
16	17011.0
17	17011.0
18	31132.5
19	45254.0
20	45254.0
21	45254.0
22	71007.0
23	96760.0
24	96760.0
25	96760.0
26	137824.5
27	178889.0
28	178889.0
29	178889.0
30	219778.0
31	260667.0
32	260667.0
33	260667.0
34	311773.0
35	362879.0
36	362879.0
37	362879.0
38	401172.0
39	439465.0
40	439465.0
41	439465.0
42	490767.5
43	542070.0
44	542070.0
45	542070.0
46	576600.5
47	611131.0
48	611131.0
49	611131.0
50	619968.5
51	628806.0
52	628806.0
53	628806.0
54	588709.0
55	548612.0
56	548612.0
57	548612.0
58	516684.0
59	484756.0
60	484756.0
61	484756.0
62	437674.0
63	390592.0
64	390592.0
65	390592.0
66	336235.0
67	281878.0
68	281878.0
69	281878.0
70	225945.0
71	170012.0
72	170012.0
73	170012.0
74	142116.0
75	114220.0
76	114220.0
77	114220.0
78	97776.0
79	81332.0
80	81332.0
81	81332.0
82	63090.0
83	44848.0
84	44848.0
85	44848.0
86	33099.0
87	21350.0
88	21350.0
89	21350.0
90	15048.5
91	8747.0
92	8747.0
93	8747.0
94	4797.0
95	847.0
96	847.0
97	847.0
98	483.5
99	120.0
100	120.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027178185575346465
2	0.0018343403487492793
3	0.005727634150176322
4	0.14927412531913778
5	0.015123948997851201
6	0.0639772991022963
7	0.03767884818400306
8	0.015423433136422512
9	0.028563299716238777
10	0.01804391934892148
11	0.02897509040677433
12	0.022891818842044578
13	0.0044548265612482495
14	0.002433308625891901
15	0.002021517935356349
16	0.0026579217298203844
17	0.0013102431062494853
18	0.0018904936247314001
19	0.0
20	0.0011979365542852437
21	9.358879330353466E-5
22	1.8717758660706933E-5
23	1.8717758660706933E-4
24	5.989682771426219E-4
25	0.11404730351968734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5342520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.552540140264014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.13805075879533	41.336686269302454
2	9.567450529675096	9.290480517640805
3	2.1349380520186187	3.1096999640283314
4	0.841306795144057	1.633903277660348
5	0.45628266116605287	1.1076841110785631
6	0.3015652178212334	0.8785054405903736
7	0.2057622133223981	0.6993194685179712
8	0.15880649151636678	0.6168366843106314
9	0.1199851081550298	0.5243023601937903
>10	0.8342160216146131	8.03809676293334
>50	0.1101837293761872	3.7520557344064316
>100	0.10370483874594348	10.415386010737025
>500	0.014770132339120839	5.1211980745362595
>1k	0.01235390915411446	11.629270690682816
>5k	5.845698337908963E-4	1.649430828058708
>10k+	3.897132225272642E-5	0.197143805322164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	10419	0.19502032748590553	No Hit
GAATAGGACCGCGGTTCTATTTTGT	7090	0.13270890890441214	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6838	0.127992033721914	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	6474	0.12117876956941667	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	5967	0.11168886592843827	No Hit
CTTATGACCCGCACTTACTGGGAAT	5926	0.11092143782334928	No Hit
ATCAGATACCGTCGTAGTTCCGACC	5854	0.10957375919977838	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	5850	0.10949888816513555	No Hit
GTATCTGATCGTCTTCGAACCTCCG	5744	0.10751480574710062	No Hit
TCGTAGTTCCGACCATAAACGATGC	5445	0.10191819590754925	No Hit
GAACTACGACGGTATCTGATCGTCT	5423	0.10150640521701369	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5414	0.10133794538906733	No Hit
GTCCTATTCCATTATTCCTAGCTGC	5402	0.10111333228513884	No Hit
GACTTAATCAACGCAAGCTTATGAC	5364	0.10040205745603199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8717758660706933E-5
2	0.0	0.0	0.0	0.0	1.8717758660706933E-5
3	0.0	0.0	0.0	0.0	1.8717758660706933E-5
4	0.0	0.0	0.0	0.0	1.8717758660706933E-5
5	0.0	0.0	0.0	0.0	1.8717758660706933E-5
6	5.61532759821208E-5	0.0	0.0	0.0	1.8717758660706933E-5
7	5.61532759821208E-5	0.0	0.0	0.0	1.8717758660706933E-5
8	5.61532759821208E-5	1.8717758660706933E-5	0.0	0.0	1.8717758660706933E-5
9	5.61532759821208E-5	1.8717758660706933E-5	0.0	1.8717758660706933E-5	1.8717758660706933E-5
10	7.487103464282773E-5	1.8717758660706933E-5	0.0	5.61532759821208E-5	1.8717758660706933E-5
11	7.487103464282773E-5	1.8717758660706933E-5	0.0	5.61532759821208E-5	1.8717758660706933E-5
12	7.487103464282773E-5	1.8717758660706933E-5	0.0	5.61532759821208E-5	1.8717758660706933E-5
13	7.487103464282773E-5	1.8717758660706933E-5	0.0	5.61532759821208E-5	3.7435517321413866E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2650	0.0	16.521824	1
ACGCTTA	245	0.0	13.561647	17
TTCGCGG	50	0.001508555	13.290415	17
GTATCAA	8240	0.0	12.960198	1
CTCGACG	260	0.0	12.779485	13
CGACCGT	145	7.2759576E-12	12.452357	19
GGTAGCG	290	0.0	11.787362	11
GTTCGAC	145	9.822543E-11	11.784604	16
TCGACGC	285	0.0	11.658259	14
AACCGCG	690	0.0	11.423257	7
CGTCGTA	1015	0.0	11.41316	10
ACCGTTA	125	1.8551873E-8	11.391785	18
CGGTAGC	285	0.0	11.327817	10
CGAACGA	565	0.0	11.257368	16
GCGCGCT	455	0.0	11.05796	15
TACCGTC	1075	0.0	11.042375	7
GCGTTAT	520	0.0	10.982316	1
ATACCGT	1090	0.0	10.980417	6
CGACCAT	910	0.0	10.643168	10
CGCTTAC	295	0.0	10.619461	18
>>END_MODULE