FastQCFastQC Report
Thu 2 Feb 2017
SRR4050254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680846
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA443906.5198297412337No Hit
CTTCCGTACTCTGCGTTGATACCAC283814.168490378147188No Hit
GAGTACGGAAGCAGTGGTATCAACG217553.195289389964838No Hit
TCCGTACTCTGCGTTGATACCACTG213663.138154590024763No Hit
GCTTCCGTACTCTGCGTTGATACCA177442.606169383384789No Hit
GCGTTGATACCACTGCTTCCGTACT105741.5530678009417695No Hit
GTATCAACGCAGAGTACGGAAGCAG101041.484036037518029No Hit
CCCATGTACTCTGCGTTGATACCAC91591.3452381302085934No Hit
GTACATGGAAGCAGTGGTATCAACG82371.2098183730241494No Hit
GTACATGGGAAGCAGTGGTATCAAC82051.2051183380676393No Hit
TATCAACGCAGAGTACGGAAGCAGT70301.0325389295082883No Hit
TCCATGTACTCTGCGTTGATACCAC67460.990826119269262No Hit
ACTCTGCGTTGATACCACTGCTTCC60540.8891878633347335No Hit
CAGTGGTATCAACGCAGAGTACGGA51030.7495086994709523No Hit
GGTATCAACGCAGAGTACGGAAGCA47540.6982489432265152No Hit
ATACCACTGCTTCCGTACTCTGCGT47390.6960458018406511No Hit
ACGCAGAGTACGGAAGCAGTGGTAT43900.6447860455962141No Hit
CATGTACTCTGCGTTGATACCACTG37220.5466728158790681No Hit
AAGCAGTGGTATCAACGCAGAGTAC33370.49012552030855727No Hit
GAAGCAGTGGTATCAACGCAGAGTA32190.4727941414064267No Hit
GTGGTATCAACGCAGAGTACGGAAG30930.4542877537651686No Hit
GTACTCTGCGTTGATACCACTGCTT29180.4285844375967547No Hit
GAGTACATGGAAGCAGTGGTATCAA27310.4011186083196494No Hit
GAGTACATGGGAAGCAGTGGTATCA26560.39010290139032905No Hit
GATACCACTGCTTCCGTACTCTGCG26360.38716537954251035No Hit
CCACTGCTTCCGTACTCTGCGTTGA25140.369246496270816No Hit
GTTGATACCACTGCTTCCGTACTCT24970.3667496027001701No Hit
GTACGGGAAGCAGTGGTATCAACGC24970.3667496027001701No Hit
ATCAACGCAGAGTACGGAAGCAGTG24080.35367763047737666No Hit
GCGTTGATACCACTGCTTCCCATGT22340.32812119040135357No Hit
CTGCTTCCGTACTCTGCGTTGATAC21670.3182804922111608No Hit
CGGAAGCAGTGGTATCAACGCAGAG21480.315489846455733No Hit
GTATCAACGCAGAGTACATGGGAAG21160.310789811499223No Hit
CCCGTACTCTGCGTTGATACCACTG20440.30021473284707556No Hit
TTCCCGTACTCTGCGTTGATACCAC20020.2940459369666562No Hit
GCTTCCCATGTACTCTGCGTTGATA17630.25894255088522217No Hit
CTTCCCGTACTCTGCGTTGATACCA17570.25806129433087655No Hit
CATGGAAGCAGTGGTATCAACGCAG16900.2482205961406838No Hit
TATCAACGCAGAGTACATGGGAAGC16640.24440181773851943No Hit
GCTTCCATGTACTCTGCGTTGATAC16280.2391142784124457No Hit
AAAAAGTACTCTGCGTTGATACCAC16260.23882052622766384No Hit
GCAGAGTACGGAAGCAGTGGTATCA15640.22971420849942573No Hit
CACTGCTTCCGTACTCTGCGTTGAT15520.22795169539073448No Hit
GTATCAACGCAGAGTACATGGAAGC14730.21634848409185042No Hit
GCGTTGATACCACTGCTTCCATGTA14550.21370471442881356No Hit
CAGTGGTATCAACGCAGAGTACATG14380.21120782085816767No Hit
GAGTACGGGAAGCAGTGGTATCAAC13530.19872335300493796No Hit
TGATACCACTGCTTCCGTACTCTGC13390.19666708771146485No Hit
ACGCAGAGTACATGGGAAGCAGTGG12770.18756076998322674No Hit
GCAGTGGTATCAACGCAGAGTACGG12290.18051071754846176No Hit
CTGCGTTGATACCACTGCTTCCGTA11810.1734606651136968No Hit
TACCACTGCTTCCGTACTCTGCGTT11540.1694950106191415No Hit
GTATCAACGCAGAGTACGGGAAGCA11180.16420747129306776No Hit
CCATGTACTCTGCGTTGATACCACT10970.16112307335285808No Hit
GCGTTGATACCACTGCTTCCCGTAC10760.15803867541264838No Hit
AAAGTACTCTGCGTTGATACCACTG10470.1537792687333112No Hit
TATCAACGCAGAGTACATGGAAGCA10250.1505479947007106No Hit
GGTATCAACGCAGAGTACATGGGAA10190.14966673814636497No Hit
GCTTCCCGTACTCTGCGTTGATACC9980.1465823402061553No Hit
CCCCATGTACTCTGCGTTGATACCA9370.13762289857030813No Hit
ACGCAGAGTACATGGAAGCAGTGGT8890.13057284613554315No Hit
TACGGAAGCAGTGGTATCAACGCAG8490.12469780243990565No Hit
CTCTGCGTTGATACCACTGCTTCCG8480.12455092634751472No Hit
TCAACGCAGAGTACGGAAGCAGTGG8350.12264153714643253No Hit
ATACCACTGCTTCCCATGTACTCTG7800.11456335206493097No Hit
CTGCTTCCCATGTACTCTGCGTTGA7780.11426959988014912No Hit
TATCAACGCAGAGTACGGGAAGCAG7310.10736642353777508No Hit
GGGAAGCAGTGGTATCAACGCAGAG7260.10663204307582037No Hit
GGTATCAACGCAGAGTACATGGAAG6930.10178513202691945No Hit
TCCCATGTACTCTGCGTTGATACCA6830.10031637110301007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA359.827717E-519.0254239
GAGTGTT453.4938275E-516.9114887
TCCGATA453.4938275E-516.9114888
GGTTAAT453.612975E-516.8455031
AATTCCG402.7286974E-416.6484745
TTCCGAT402.7302367E-416.6472457
CGATAAC402.7302367E-416.64724510
GCATGTC402.7425773E-416.6374112
CAAGACG402.843046E-416.5591324
CGAACGA350.00215984916.29546216
GCCCCGT350.00220608416.243881
TAATTCC551.1696273E-515.4838644
TTGAACA808.103598E-915.3763374
ATTCCGA508.598242E-515.2214646
CACTCTA508.6036656E-515.2203399
CTCTAAT508.6036656E-515.22033911
GATAACG456.6872116E-414.79755111
GTTAATT456.965686E-414.7181462
TGTACTA400.005225876314.2701215
TACCGTC400.005228440314.2690677
Produced by FastQC (version 0.11.5)