##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050254_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680846
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97128866145942	31.0	31.0	34.0	28.0	34.0
2	31.090894269776133	31.0	31.0	34.0	28.0	34.0
3	31.212667769216534	33.0	31.0	34.0	28.0	34.0
4	34.80262790704506	37.0	35.0	37.0	32.0	37.0
5	34.81093521883069	37.0	35.0	37.0	32.0	37.0
6	34.85269209189743	37.0	35.0	37.0	32.0	37.0
7	34.735194155506534	37.0	35.0	37.0	32.0	37.0
8	34.70501552480297	37.0	35.0	37.0	32.0	37.0
9	36.257111006013105	39.0	37.0	39.0	32.0	39.0
10	36.21992785446342	39.0	37.0	39.0	32.0	39.0
11	36.07960243579311	39.0	37.0	39.0	32.0	39.0
12	36.051206880851176	39.0	37.0	39.0	32.0	39.0
13	36.00577663671373	39.0	37.0	39.0	32.0	39.0
14	37.24440769278222	40.0	38.0	41.0	32.0	41.0
15	37.18705404746448	40.0	38.0	41.0	32.0	41.0
16	37.03821128419643	40.0	38.0	41.0	32.0	41.0
17	37.12628112671588	40.0	38.0	41.0	32.0	41.0
18	37.04791979390347	40.0	38.0	41.0	32.0	41.0
19	37.1497240198224	40.0	38.0	41.0	32.0	41.0
20	37.15850574138645	40.0	38.0	41.0	32.0	41.0
21	37.12525886911284	40.0	38.0	41.0	32.0	41.0
22	37.098527420297685	40.0	38.0	41.0	32.0	41.0
23	37.02749520449559	40.0	38.0	41.0	32.0	41.0
24	36.95272499214213	40.0	38.0	41.0	31.0	41.0
25	36.73213178898018	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39770910912804425
1101	2	0.4096557316626317
1101	3	0.34005938700131466
1101	4	0.35401349169701746
1101	5	0.3302679606638037
1101	6	0.46892538509906956
1101	7	0.3747789182704224
1101	8	0.32142262360337526
1101	9	0.29675880491394224
1101	10	0.417373946948679
1101	11	0.2292447910760984
1101	12	0.4292776602724331
1101	13	0.5991699597414168
1101	14	0.6683385896783918
1101	15	0.7247859132664161
1101	16	0.6550041302377707
1101	17	0.5916597757188882
1101	18	0.5947045462833742
1101	19	0.736115286955453
1101	20	0.5094390113573724
1101	21	0.5642957670485771
1101	22	0.6508997642185861
1101	23	0.6957055553324452
1101	24	0.7242403189855864
1101	25	0.6296508675637114
1102	1	0.36404267036692417
1102	2	0.30098524675229044
1102	3	0.3917989008688707
1102	4	0.24571954638774685
1102	5	0.3549469936836829
1102	6	0.24052769322076273
1102	7	0.3423799976839419
1102	8	0.3462494649315744
1102	9	0.39573834956526355
1102	10	0.28133580414768744
1102	11	0.20229413427873055
1102	12	0.30695183461026687
1102	13	0.4061100139763312
1102	14	0.49404864757961064
1102	15	0.5443243549870047
1102	16	0.5319582551630404
1102	17	0.4896654370248186
1102	18	0.4739814911599609
1102	19	0.48641833527472755
1102	20	0.4350996897231951
1102	21	0.5211373925105818
1102	22	0.4302615956543647
1102	23	0.453749134836805
1102	24	0.5014692517600494
1102	25	0.7274628091538275
1103	1	0.5268002882192206
1103	2	0.5465778185847192
1103	3	0.4543055012474291
1103	4	0.598806086489617
1103	5	0.5619925423883885
1103	6	0.5485610647347485
1103	7	0.4999645434560449
1103	8	0.5425987447794967
1103	9	0.6879527461078823
1103	10	0.6286302582049927
1103	11	0.69485215668346
1103	12	0.576025507020276
1103	13	0.735478246049702
1103	14	0.7663684877082844
1103	15	0.7671084555889607
1103	16	0.7143447895784334
1103	17	0.8089352929944056
1103	18	0.873513575092403
1103	19	0.7606277114678761
1103	20	0.5273530792714425
1103	21	0.7727998755526855
1103	22	0.9009591642779924
1103	23	0.7866470462739414
1103	24	0.8551382498835167
1103	25	0.8432980312108711
1104	1	0.2990456733502711
1104	2	0.46953852884125524
1104	3	0.28410717224692306
1104	4	0.3396556474843635
1104	5	0.4486458018184152
1104	6	0.34178060005954336
1104	7	0.4118610134287408
1104	8	0.4917722376916487
1104	9	0.4542629764834487
1104	10	0.4297224574823062
1104	11	0.6540402013524513
1104	12	0.5113960863110023
1104	13	0.5847024248564594
1104	14	0.6674377965815879
1104	15	0.6513729378570687
1104	16	0.7623181713086211
1104	17	0.7984433128109529
1104	18	0.6905293204203105
1104	19	0.8107315358003717
1104	20	0.7202096381897078
1104	21	0.788453641841663
1104	22	0.8973032397509186
1104	23	0.9305034253390261
1104	24	0.8907540835029764
1104	25	0.9443052089875579
1105	1	0.3643001874022005
1105	2	0.3483102582759763
1105	3	0.39364188328748284
1105	4	0.3705257022557831
1105	5	0.37514795384281285
1105	6	0.4440517210132242
1105	7	0.3561228503753213
1105	8	0.3091694336420403
1105	9	0.5182664080240542
1105	10	0.5149747943257097
1105	11	0.6919228173172414
1105	12	0.6573316116925412
1105	13	0.6001922886509448
1105	14	0.6097157741173476
1105	15	0.6305578494770288
1105	16	0.6175378662719595
1105	17	0.5954096814383547
1105	18	0.490852489656028
1105	19	0.5884588550555065
1105	20	0.5292895726134859
1105	21	0.4448283946716245
1105	22	0.514231523476937
1105	23	0.5469751451875311
1105	24	0.5109416808883793
1105	25	0.4791340462769682
1106	1	0.22802609104787308
1106	2	0.35486642776379895
1106	3	0.19175666567989325
1106	4	0.2510310630244774
1106	5	0.19509643880483907
1106	6	0.18784612004829881
1106	7	0.28348760347064683
1106	8	0.18531251821455186
1106	9	0.3334157514106053
1106	10	0.3427390241392274
1106	11	0.41097772125070264
1106	12	0.3482740690284132
1106	13	0.4589883099871983
1106	14	0.45918695176450797
1106	15	0.4250589260950832
1106	16	0.3719286423537369
1106	17	0.4989740405576413
1106	18	0.5672393578653825
1106	19	0.4723610833081011
1106	20	0.41784027171767235
1106	21	0.34734842231851104
1106	22	0.3221941124942376
1106	23	0.439709547778115
1106	24	0.5727214608772329
1106	25	0.5494390787588657
1107	1	0.3289984864174187
1107	2	0.42873998074687947
1107	3	0.4167919877339159
1107	4	0.19577905946258767
1107	5	0.36572827812412356
1107	6	0.346903407077086
1107	7	0.2798924713839739
1107	8	0.1277278739086256
1107	9	0.14749628965142847
1107	10	0.2812909188656505
1107	11	0.45014344797475303
1107	12	0.42495043194520576
1107	13	0.3328476154190767
1107	14	0.6446267459665478
1107	15	0.6773426898231918
1107	16	0.605900345133989
1107	17	0.5369833410424576
1107	18	0.6301502117290383
1107	19	0.7246036874438531
1107	20	0.6461398660582276
1107	21	0.5749380136908258
1107	22	0.7445627678815967
1107	23	0.6450974967243894
1107	24	0.874946057691325
1107	25	0.762337070249913
1108	1	0.13392705784599102
1108	2	0.2667756950325959
1108	3	0.12591103535296355
1108	4	0.14677905946258818
1108	5	0.07014891304475412
1108	6	0.11471293088661128
1108	7	0.05676151900301818
1108	8	0.15198581041656212
1108	9	0.14478994044507942
1108	10	0.18800123632596666
1108	11	0.212234717816024
1108	12	0.08676392400869304
1108	13	0.011097615419075169
1108	14	0.01549976183956403
1108	15	-0.01353826255775914
1108	16	0.05244002767366851
1108	17	-0.08061586530675413
1108	18	0.09030894188776983
1108	19	-0.09831694747678199
1108	20	0.09529065970902195
1108	21	0.1834578549606647
1108	22	0.20277705359588083
1108	23	0.24364511577201142
1108	24	0.19816431165957482
1108	25	0.2617021496149903
1109	1	-0.003822474281268029
1109	2	-0.11134735549766006
1109	3	0.04661440403668138
1109	4	-0.16097203224047263
1109	5	-0.13977778689286424
1109	6	-0.22845612712960417
1109	7	-0.02698380226959074
1109	8	-0.003866497755616649
1109	9	-0.04278803834468903
1109	10	0.10918417456677076
1109	11	0.10501778082288382
1109	12	0.09575877740856953
1109	13	0.060742811925621254
1109	14	-0.15494627683791862
1109	15	-0.21772572356836406
1109	16	-0.025975443318216662
1109	17	-0.09284683848204622
1109	18	-0.048898793608174174
1109	19	0.00729752538659767
1109	20	0.07915684810578227
1109	21	-0.17804791546977583
1109	22	-0.1906337386686232
1109	23	-0.1824075978898776
1109	24	0.027768959195448417
1109	25	0.09407894312715825
1110	1	0.048435986417420196
1110	2	0.036344147413547745
1110	3	-0.005388567821640322
1110	4	0.01900128168480819
1110	5	-0.022410610764765693
1110	6	-0.07333270403402281
1110	7	-0.02002419528269428
1110	8	-0.08625823720248604
1110	9	0.03410045631809311
1110	10	-0.04039658113434541
1110	11	0.022428170196974406
1110	12	0.09678376527853771
1110	13	-0.10192321791426195
1110	14	0.11021702374432607
1110	15	0.2313774120454113
1110	16	0.17545590068954198
1110	17	0.21514306326467647
1110	18	0.14383076728459798
1110	19	0.1782703541105164
1110	20	0.014313477169338285
1110	21	0.18333384702415856
1110	22	0.05820860121492899
1110	23	-0.059951114386720405
1110	24	0.04454327991354745
1110	25	0.20560095913879906
1111	1	-0.3236174305375954
1111	2	-0.3533707458967186
1111	3	-0.446462914226867
1111	4	-0.3887745737554056
1111	5	-0.29508217939221737
1111	6	-0.4289351165676649
1111	7	-0.33219980081902634
1111	8	-0.2847696270525404
1111	9	-0.3596032874343109
1111	10	-0.24517659362954447
1111	11	-0.3083009964696899
1111	12	-0.2712717902770194
1111	13	-0.3454984053421697
1111	14	-0.3684720621841677
1111	15	-0.3513101360130264
1111	16	-0.37712003164417496
1111	17	-0.34570793154501445
1111	18	-0.32395205662928106
1111	19	-0.42491111455537833
1111	20	-0.430938949781833
1111	21	-0.4630912058401222
1111	22	-0.34457218021529457
1111	23	-0.4785311076585401
1111	24	-0.4294030388100225
1111	25	-0.2957867282891087
1112	1	-0.28421770651970135
1112	2	-0.20667776257783999
1112	3	-0.2947807943504941
1112	4	-0.37516323166575205
1112	5	-0.32664726020490775
1112	6	-0.5624988323716593
1112	7	-0.3976889239648642
1112	8	-0.558763082163722
1112	9	-0.4641970781349656
1112	10	-0.4371518029259107
1112	11	-0.3201442350571142
1112	12	-0.28483768174988455
1112	13	-0.47749519250513117
1112	14	-0.4497057260403423
1112	15	-0.35395446779954653
1112	16	-0.42467329827686484
1112	17	-0.5557642903098312
1112	18	-0.6000995729393495
1112	19	-0.37200957698336623
1112	20	-0.42548115030697176
1112	21	-0.5409138381610248
1112	22	-0.5476336145473439
1112	23	-0.6531695144728573
1112	24	-0.530593993988262
1112	25	-0.4826930761755861
1113	1	-0.08534034937754242
1113	2	0.05991287388328459
1113	3	-0.09034036094838882
1113	4	0.030163073363539183
1113	5	0.06429841037626716
1113	6	-0.007503775083350206
1113	7	-0.12713214489173197
1113	8	-0.14566762758765606
1113	9	-0.13594265233138714
1113	10	-0.1957559003253877
1113	11	0.007728543061155335
1113	12	0.06039632440586473
1113	13	-0.16022623340803221
1113	14	-0.043265907764507006
1113	15	-0.05846037997118714
1113	16	-0.0789364151710572
1113	17	-0.1433834897327202
1113	18	-0.1435808433921082
1113	19	-0.13074837356590052
1113	20	-0.2366179290957433
1113	21	-0.20301120920714055
1113	22	-0.1571227229959078
1113	23	-0.2832616112976183
1113	24	-0.18745456012893413
1113	25	-0.13505843124637096
1114	1	-0.13620369937492782
1114	2	0.03897677491810114
1114	3	0.09561894583956132
1114	4	0.18908324889792283
1114	5	0.15000514515508456
1114	6	0.25137699541952685
1114	7	0.1637758957919857
1114	8	0.10178251871737132
1114	9	0.02603180877711253
1114	10	0.28182279500408214
1114	11	0.4845041037124602
1114	12	0.2701671896865534
1114	13	0.39555799793273394
1114	14	0.318251518279844
1114	15	0.32465780822028023
1114	16	0.26425371489719396
1114	17	0.20525292209892143
1114	18	0.2205327253902425
1114	19	0.18578401531270572
1114	20	0.2471089006666105
1114	21	0.18616570039392144
1114	22	0.11221304110564034
1114	23	0.33928328907411753
1114	24	0.2953686442122674
1114	25	0.32914945640656157
1115	1	0.2776637316199775
1115	2	0.2264372088918698
1115	3	0.25813384985189813
1115	4	0.2442737289721819
1115	5	0.2543973138976341
1115	6	0.2300647432533509
1115	7	-0.07566118888610873
1115	8	0.17028271873213185
1115	9	0.02024303212951395
1115	10	0.12785997833023544
1115	11	0.1559847178160254
1115	12	0.4211589133477176
1115	13	0.07201179452355433
1115	14	0.1422055187692024
1115	15	0.09373785684522318
1115	16	0.10894322596791284
1115	17	0.11374714108983852
1115	18	-0.01768679371990345
1115	19	0.16692825508184939
1115	20	0.2446776533124364
1115	21	0.08397757777516546
1115	22	0.12311287449140451
1115	23	0.07277624583597486
1115	24	0.35550706219262196
1115	25	0.24805609417789043
1116	1	-0.008506668221755831
1116	2	0.06080183641698156
1116	3	0.19165109426312554
1116	4	0.23105740997804958
1116	5	0.013134921881281514
1116	6	-0.11562580254490484
1116	7	-0.1176401746641389
1116	8	0.13157896211023257
1116	9	0.24340923352313126
1116	10	0.05137568404320092
1116	11	0.3257196220870142
1116	12	0.24419212724875194
1116	13	0.3321672030479377
1116	14	0.3231949017511937
1116	15	0.11302653862044565
1116	16	0.1614238273791173
1116	17	0.2658035014090032
1116	18	0.41593486235905175
1116	19	0.28923828080009173
1116	20	0.2873930161155016
1116	21	0.1293801671845216
1116	22	0.005078231799117816
1116	23	0.07094629397524699
1116	24	-0.11150984150684451
1116	25	-0.030492253919618406
1201	1	0.5848895934954612
1201	2	0.527614753886624
1201	3	0.5726734154411162
1201	4	0.4703271538364504
1201	5	0.5756875441051648
1201	6	0.6276245202047335
1201	7	0.6042385088915339
1201	8	0.6415000053867459
1201	9	0.7909235935433401
1201	10	0.7264556698235083
1201	11	0.8945120706809462
1201	12	0.8925213131712582
1201	13	0.8469528785769711
1201	14	1.005005854657817
1201	15	0.9562731193452763
1201	16	0.9120459390037183
1201	17	0.9654124598163989
1201	18	0.9645765073531578
1201	19	0.9649965085771441
1201	20	0.933458277028187
1201	21	0.9281995583570861
1201	22	1.0542905355766905
1201	23	0.9450224816003754
1201	24	1.0795018149004392
1201	25	0.990584700820591
1202	1	0.2223318197507531
1202	2	0.27229553630243686
1202	3	0.1767919877339139
1202	4	0.4411123927959224
1202	5	0.29372827812412083
1202	6	0.18957007374375223
1202	7	0.27811469360619157
1202	8	0.2752834294641815
1202	9	0.3474962896514313
1202	10	0.3755131410878718
1202	11	0.559476781308085
1202	12	0.3165059875007614
1202	13	0.37195872653018824
1202	14	0.19129341263321464
1202	15	0.32800935648985785
1202	16	0.16856701180065414
1202	17	0.44187222993134156
1202	18	0.3110391006179256
1202	19	0.33260368744385005
1202	20	0.3119176438360114
1202	21	0.2024935692463785
1202	22	0.1810072123260369
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2210	14	-0.09806167672187627
2210	15	-0.1347295712490677
2210	16	0.1147527149863592
2210	17	-0.06922101016189686
2210	18	0.03326598484481025
2210	19	-0.010055209215046546
2210	20	-0.015036513118147354
2210	21	-0.012002934250126884
2210	22	0.07152080983963316
2210	23	0.21343782306471581
2210	24	0.10115507090033304
2210	25	0.23288097079381487
2211	1	-0.5595764478507661
2211	2	-0.47250454160193556
2211	3	-0.5045775711325753
2211	4	-0.6809816767337296
2211	5	-0.6325570466397323
2211	6	-0.5900197597356467
2211	7	-0.6677838359487751
2211	8	-0.740230447252145
2211	9	-0.6327845556110105
2211	10	-0.7476747058300361
2211	11	-0.8427006459000168
2211	12	-0.8012893187608086
2211	13	-0.97587105241616
2211	14	-0.8724713161621906
2211	15	-0.7770680609801985
2211	16	-0.7972532238600749
2211	17	-0.785123972230501
2211	18	-1.0375175261227483
2211	19	-1.0281153699132446
2211	20	-0.9629367723681028
2211	21	-0.9801321976247834
2211	22	-0.9709849972367266
2211	23	-0.9410503268222072
2211	24	-0.937336345658565
2211	25	-0.8058701553806245
2212	1	-0.3485596324823135
2212	2	-0.4231145001759238
2212	3	-0.476096506794331
2212	4	-0.3490727595258747
2212	5	-0.3746270408150494
2212	6	-0.2905222066466635
2212	7	-0.3359347986544776
2212	8	-0.3875050946411136
2212	9	-0.6260507871503052
2212	10	-0.4347749393616951
2212	11	-0.5427730461591338
2212	12	-0.6379532454677914
2212	13	-0.6020734138622075
2212	14	-0.5840787652234383
2212	15	-0.5832476680590943
2212	16	-0.5815440007202923
2212	17	-0.4340153772854549
2212	18	-0.3701613331787783
2212	19	-0.4481838507598326
2212	20	-0.4608872462359628
2212	21	-0.5594159249860979
2212	22	-0.48496695705186
2212	23	-0.6214059046361555
2212	24	-0.5026952713040416
2212	25	-0.49757025499902596
2213	1	-0.24219728077834546
2213	2	-0.3126004072601738
2213	3	-0.33257661896802304
2213	4	-0.49224210455857786
2213	5	-0.5273158135866396
2213	6	-0.3001018839281997
2213	7	-0.41073186724036503
2213	8	-0.2827694805887262
2213	9	-0.40236614421100825
2213	10	-0.3867231904817885
2213	11	-0.2734685449705694
2213	12	-0.3360866050918361
2213	13	-0.6468278695897425
2213	14	-0.47784238983592076
2213	15	-0.4536837652032659
2213	16	-0.5674894255891161
2213	17	-0.2680607506683046
2213	18	-0.3910843561721933
2213	19	-0.42865204447854666
2213	20	-0.5463539611022625
2213	21	-0.659079622993481
2213	22	-0.42171589173040047
2213	23	-0.7112270182667899
2213	24	-0.5546906266120288
2213	25	-0.5226012013550232
2214	1	-0.5616803456993686
2214	2	-0.6478293623188129
2214	3	-0.5963126351371315
2214	4	-0.7862501376177065
2214	5	-0.5872676667258361
2214	6	-0.8643277364752251
2214	7	-0.69589098360386
2214	8	-0.7553199768618484
2214	9	-0.7590552107540844
2214	10	-0.713608513413341
2214	11	-0.8493593906302763
2214	12	-0.5988265348025692
2214	13	-0.7259918006393207
2214	14	-0.8303813643335332
2214	15	-0.7656889403471254
2214	16	-0.7516527773315431
2214	17	-0.6253564805309466
2214	18	-0.5800768766732318
2214	19	-0.5125244552974308
2214	20	-0.6517765978509331
2214	21	-0.6955502263740598
2214	22	-0.5972620496366545
2214	23	-0.6189852283364417
2214	24	-0.8255778676939158
2214	25	-0.7073020213964796
2215	1	-0.7663922420593998
2215	2	-0.8120105711294983
2215	3	-0.9035788731932364
2215	4	-0.9143136557691989
2215	5	-0.8741628182702854
2215	6	-0.7408846723931077
2215	7	-0.7641605087484749
2215	8	-0.8373964822355973
2215	9	-0.9372307154994175
2215	10	-1.030870964872392
2215	11	-1.2315901796926454
2215	12	-1.3170775297913693
2215	13	-1.1507815236537766
2215	14	-1.0388758294565577
2215	15	-1.2050590761812217
2215	16	-1.0554123848145025
2215	17	-1.299602383755193
2215	18	-1.2595333791466032
2215	19	-1.5826751940866828
2215	20	-1.266286918342061
2215	21	-1.3167293888110123
2215	22	-1.4353930820080336
2215	23	-1.515220384070311
2215	24	-1.20739978483995
2215	25	-1.3267872858943122
2216	1	-0.5885785370812755
2216	2	-0.3993540140311893
2216	3	-0.5992428251468489
2216	4	-0.4835734209551674
2216	5	-0.5660083044114188
2216	6	-0.7197145215565044
2216	7	-0.5142311143427492
2216	8	-0.48411778898664437
2216	9	-0.8233049868208653
2216	10	-0.6160760669190921
2216	11	-0.5622174455558522
2216	12	-0.4677469860994705
2216	13	-0.6008965099072938
2216	14	-0.3510332482313103
2216	15	-0.5648017836319852
2216	16	-0.7317822774363663
2216	17	-0.7160946978319274
2216	18	-0.6224288425210318
2216	19	-0.9192419754514205
2216	20	-0.6414447002312968
2216	21	-0.7366395900225484
2216	22	-0.8996147778103136
2216	23	-0.6039817025793539
2216	24	-0.7092326484299463
2216	25	-0.9330386187550204
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11639.0
3	1.0
4	96.0
5	624.0
6	1202.0
7	1293.0
8	1151.0
9	1052.0
10	934.0
11	1443.0
12	1739.0
13	1367.0
14	586.0
15	561.0
16	517.0
17	621.0
18	720.0
19	969.0
20	1234.0
21	1615.0
22	2033.0
23	2251.0
24	2726.0
25	3026.0
26	3471.0
27	3714.0
28	4267.0
29	5417.0
30	6808.0
31	8976.0
32	12144.0
33	16832.0
34	24856.0
35	37438.0
36	60714.0
37	123814.0
38	332995.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.39086018104274	13.789760330955424	13.382980570197828	23.43639891780401
2	10.23108225235416	21.298644062816766	40.35051637261095	28.119757312218123
3	21.059173371101487	29.88522634446201	27.518791027321182	21.53680925711532
4	13.448152735960925	13.944224278411339	32.710598974060574	39.89702401156717
5	17.69332144258133	31.810602064818312	29.403606306408225	21.092470186192134
6	38.22144953435432	29.281963633050335	13.323330193268307	19.173256639327036
7	26.131303184844434	32.380182536999754	22.065078408606574	19.423435869549245
8	25.56773671067658	34.5076332680264	24.143379332340746	15.78125068895627
9	28.89020490695132	20.607520308477245	14.595799583837863	35.906475200733574
10	13.462069695135211	24.573148153590257	30.3656966868656	31.599085464408933
11	31.437700058225627	25.95355545769184	17.76753492798643	24.841209556096107
12	26.803202542657	22.45486916747774	25.59833522132311	25.143593068542152
13	32.352893405212576	19.18044672032508	27.699790475262347	20.766869399199997
14	32.725015206722844	18.123460872954475	22.66510367146392	26.486420248858757
15	34.90479811589602	18.05048394598705	26.02426005359951	21.02045788451742
16	28.298460929819225	20.50228661011895	31.786537785900077	19.412714674161748
17	24.131516944498575	29.181650693546384	28.070913567999433	18.615918793955604
18	27.12484923996274	17.53569894346185	38.03684966511712	17.302602151458295
19	24.270737973599505	33.90736201754127	18.54649617576706	23.275403833092163
20	19.01399301382766	29.25260357458167	29.09610858980302	22.637294821787645
21	20.649469725716123	33.84586536658541	20.55792857132311	24.946736336375356
22	19.486890296560816	30.594081184058435	17.805606444650792	32.11342207472996
23	24.34446644196714	24.182363892173754	18.269311684227777	33.20385798163133
24	17.979995985026513	28.81835787584285	19.728133155414902	33.47351298371574
25	26.816357439245632	28.621090249925825	19.006230800911077	25.556321509917467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	186.0
2	245.0
3	304.0
4	304.0
5	304.0
6	641.0
7	978.0
8	978.0
9	978.0
10	1103.0
11	1228.0
12	1228.0
13	1228.0
14	1593.5
15	1959.0
16	1959.0
17	1959.0
18	3079.0
19	4199.0
20	4199.0
21	4199.0
22	5364.5
23	6530.0
24	6530.0
25	6530.0
26	8928.5
27	11327.0
28	11327.0
29	11327.0
30	13629.0
31	15931.0
32	15931.0
33	15931.0
34	19582.5
35	23234.0
36	23234.0
37	23234.0
38	26525.5
39	29817.0
40	29817.0
41	29817.0
42	36178.0
43	42539.0
44	42539.0
45	42539.0
46	82212.0
47	121885.0
48	121885.0
49	121885.0
50	198262.5
51	274640.0
52	274640.0
53	274640.0
54	169094.0
55	63548.0
56	63548.0
57	63548.0
58	46268.5
59	28989.0
60	28989.0
61	28989.0
62	24913.5
63	20838.0
64	20838.0
65	20838.0
66	17322.5
67	13807.0
68	13807.0
69	13807.0
70	10653.0
71	7499.0
72	7499.0
73	7499.0
74	6234.5
75	4970.0
76	4970.0
77	4970.0
78	4275.5
79	3581.0
80	3581.0
81	3581.0
82	2655.5
83	1730.0
84	1730.0
85	1730.0
86	1268.5
87	807.0
88	807.0
89	807.0
90	545.5
91	284.0
92	284.0
93	284.0
94	155.0
95	26.0
96	26.0
97	26.0
98	18.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16406059520067678
2	0.019975148565167453
3	0.029668970662969307
4	0.04391595162489021
5	0.030109598940142118
6	0.057722304309638296
7	0.0640379762824486
8	0.06888488733134952
9	0.05023162359770051
10	0.04141905805424428
11	0.612179553085426
12	0.5521072312975328
13	0.5286070565149829
14	0.5161225886617531
15	0.5289008086997647
16	0.5029037403465688
17	0.5078975274878608
18	0.5059881382867785
19	0.5266976673139007
20	0.5152413321074075
21	0.5221445084497816
22	0.5062818904715604
23	0.5136256950911072
24	0.49673494446614946
25	0.5001130945911411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	680846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.936232134710494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57456282680405	29.831848267635387
2	5.62036678271245	3.702274100752665
3	1.230895777634386	1.2162320719740336
4	0.5266341200964835	0.6938137451822715
5	0.32621369708896486	0.537212502642214
6	0.21492357673527007	0.42472636887450715
7	0.16497991043737353	0.38036716394103576
8	0.12659927523362102	0.33357624937445174
9	0.09113433953073427	0.27014595860050084
>10	0.8323373640811088	5.644213596736101
>50	0.14366220862709417	3.2999497039889607
>100	0.11143890949577076	7.468408080684798
>500	0.01029355388916758	2.3892832783290547
>1k	0.01969201613579885	13.61238465690994
>5k	0.00313282074887709	7.448950656245323
>10k+	0.00313282074887709	22.7466135981288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	44390	6.5198297412337	No Hit
CTTCCGTACTCTGCGTTGATACCAC	28381	4.168490378147188	No Hit
GAGTACGGAAGCAGTGGTATCAACG	21755	3.195289389964838	No Hit
TCCGTACTCTGCGTTGATACCACTG	21366	3.138154590024763	No Hit
GCTTCCGTACTCTGCGTTGATACCA	17744	2.606169383384789	No Hit
GCGTTGATACCACTGCTTCCGTACT	10574	1.5530678009417695	No Hit
GTATCAACGCAGAGTACGGAAGCAG	10104	1.484036037518029	No Hit
CCCATGTACTCTGCGTTGATACCAC	9159	1.3452381302085934	No Hit
GTACATGGAAGCAGTGGTATCAACG	8237	1.2098183730241494	No Hit
GTACATGGGAAGCAGTGGTATCAAC	8205	1.2051183380676393	No Hit
TATCAACGCAGAGTACGGAAGCAGT	7030	1.0325389295082883	No Hit
TCCATGTACTCTGCGTTGATACCAC	6746	0.990826119269262	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6054	0.8891878633347335	No Hit
CAGTGGTATCAACGCAGAGTACGGA	5103	0.7495086994709523	No Hit
GGTATCAACGCAGAGTACGGAAGCA	4754	0.6982489432265152	No Hit
ATACCACTGCTTCCGTACTCTGCGT	4739	0.6960458018406511	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	4390	0.6447860455962141	No Hit
CATGTACTCTGCGTTGATACCACTG	3722	0.5466728158790681	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3337	0.49012552030855727	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	3219	0.4727941414064267	No Hit
GTGGTATCAACGCAGAGTACGGAAG	3093	0.4542877537651686	No Hit
GTACTCTGCGTTGATACCACTGCTT	2918	0.4285844375967547	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2731	0.4011186083196494	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2656	0.39010290139032905	No Hit
GATACCACTGCTTCCGTACTCTGCG	2636	0.38716537954251035	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2514	0.369246496270816	No Hit
GTTGATACCACTGCTTCCGTACTCT	2497	0.3667496027001701	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2497	0.3667496027001701	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	2408	0.35367763047737666	No Hit
GCGTTGATACCACTGCTTCCCATGT	2234	0.32812119040135357	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2167	0.3182804922111608	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2148	0.315489846455733	No Hit
GTATCAACGCAGAGTACATGGGAAG	2116	0.310789811499223	No Hit
CCCGTACTCTGCGTTGATACCACTG	2044	0.30021473284707556	No Hit
TTCCCGTACTCTGCGTTGATACCAC	2002	0.2940459369666562	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1763	0.25894255088522217	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1757	0.25806129433087655	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1690	0.2482205961406838	No Hit
TATCAACGCAGAGTACATGGGAAGC	1664	0.24440181773851943	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1628	0.2391142784124457	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1626	0.23882052622766384	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1564	0.22971420849942573	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1552	0.22795169539073448	No Hit
GTATCAACGCAGAGTACATGGAAGC	1473	0.21634848409185042	No Hit
GCGTTGATACCACTGCTTCCATGTA	1455	0.21370471442881356	No Hit
CAGTGGTATCAACGCAGAGTACATG	1438	0.21120782085816767	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1353	0.19872335300493796	No Hit
TGATACCACTGCTTCCGTACTCTGC	1339	0.19666708771146485	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1277	0.18756076998322674	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1229	0.18051071754846176	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1181	0.1734606651136968	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1154	0.1694950106191415	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1118	0.16420747129306776	No Hit
CCATGTACTCTGCGTTGATACCACT	1097	0.16112307335285808	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1076	0.15803867541264838	No Hit
AAAGTACTCTGCGTTGATACCACTG	1047	0.1537792687333112	No Hit
TATCAACGCAGAGTACATGGAAGCA	1025	0.1505479947007106	No Hit
GGTATCAACGCAGAGTACATGGGAA	1019	0.14966673814636497	No Hit
GCTTCCCGTACTCTGCGTTGATACC	998	0.1465823402061553	No Hit
CCCCATGTACTCTGCGTTGATACCA	937	0.13762289857030813	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	889	0.13057284613554315	No Hit
TACGGAAGCAGTGGTATCAACGCAG	849	0.12469780243990565	No Hit
CTCTGCGTTGATACCACTGCTTCCG	848	0.12455092634751472	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	835	0.12264153714643253	No Hit
ATACCACTGCTTCCCATGTACTCTG	780	0.11456335206493097	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	778	0.11426959988014912	No Hit
TATCAACGCAGAGTACGGGAAGCAG	731	0.10736642353777508	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	726	0.10663204307582037	No Hit
GGTATCAACGCAGAGTACATGGAAG	693	0.10178513202691945	No Hit
TCCCATGTACTCTGCGTTGATACCA	683	0.10031637110301007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4687609239093715E-4	0.0
11	0.0	0.0	0.0	1.4687609239093715E-4	0.0
12	0.0	0.0	0.0	8.81256554345623E-4	0.0
13	0.0	0.0	0.0	0.0014687609239093715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAA	35	9.827717E-5	19.025423	9
GAGTGTT	45	3.4938275E-5	16.911488	7
TCCGATA	45	3.4938275E-5	16.911488	8
GGTTAAT	45	3.612975E-5	16.845503	1
AATTCCG	40	2.7286974E-4	16.648474	5
TTCCGAT	40	2.7302367E-4	16.647245	7
CGATAAC	40	2.7302367E-4	16.647245	10
GCATGTC	40	2.7425773E-4	16.63741	12
CAAGACG	40	2.843046E-4	16.559132	4
CGAACGA	35	0.002159849	16.295462	16
GCCCCGT	35	0.002206084	16.24388	1
TAATTCC	55	1.1696273E-5	15.483864	4
TTGAACA	80	8.103598E-9	15.376337	4
ATTCCGA	50	8.598242E-5	15.221464	6
CACTCTA	50	8.6036656E-5	15.220339	9
CTCTAAT	50	8.6036656E-5	15.220339	11
GATAACG	45	6.6872116E-4	14.797551	11
GTTAATT	45	6.965686E-4	14.718146	2
TGTACTA	40	0.0052258763	14.270121	5
TACCGTC	40	0.0052284403	14.269067	7
>>END_MODULE