FastQCFastQC Report
Thu 2 Feb 2017
SRR4050254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680846
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA388095.70011426959988No Hit
CTTCCGTACTCTGCGTTGATACCAC243913.5824547695073483No Hit
GAGTACGGAAGCAGTGGTATCAACG218553.2099769992039318No Hit
TCCGTACTCTGCGTTGATACCACTG209483.0767603834053516No Hit
GCTTCCGTACTCTGCGTTGATACCA157832.3181453662061613No Hit
GTATCAACGCAGAGTACGGAAGCAG97661.4343919182898923No Hit
GCGTTGATACCACTGCTTCCGTACT96911.423376211360572No Hit
CCCATGTACTCTGCGTTGATACCAC93171.3684445528063616No Hit
TATCAACGCAGAGTACGGAAGCAGT84741.2446280069208016No Hit
TCCATGTACTCTGCGTTGATACCAC76921.1297709026710887No Hit
ACTCTGCGTTGATACCACTGCTTCC64610.948966432937845No Hit
GTACATGGGAAGCAGTGGTATCAAC64140.942063256595471No Hit
GTACATGGAAGCAGTGGTATCAACG63460.9320756823128872No Hit
CAGTGGTATCAACGCAGAGTACGGA58120.8536438489761269No Hit
ACGCAGAGTACGGAAGCAGTGGTAT54730.803852853655599No Hit
GGTATCAACGCAGAGTACGGAAGCA51110.7506837082100798No Hit
ATACCACTGCTTCCGTACTCTGCGT47330.6951645452863056No Hit
CATGTACTCTGCGTTGATACCACTG40520.5951419263680774No Hit
AAGCAGTGGTATCAACGCAGAGTAC40340.5924981567050405No Hit
GTGGTATCAACGCAGAGTACGGAAG37560.55166660302036No Hit
GAAGCAGTGGTATCAACGCAGAGTA37190.5462321876018953No Hit
ATCAACGCAGAGTACGGAAGCAGTG30180.4432720468358483No Hit
GATACCACTGCTTCCGTACTCTGCG26770.3931872993305388No Hit
GAGTACATGGGAAGCAGTGGTATCA24580.3610214350969235No Hit
CGGAAGCAGTGGTATCAACGCAGAG24350.357643284971932No Hit
GAGTACATGGAAGCAGTGGTATCAA23870.350593232537167No Hit
CCACTGCTTCCGTACTCTGCGTTGA23410.34383693228718387No Hit
GTTGATACCACTGCTTCCGTACTCT23280.3419275430861017No Hit
GCGTTGATACCACTGCTTCCCATGT21560.3166648551948605No Hit
GTACGGGAAGCAGTGGTATCAACGC21110.31005543103726835No Hit
CTGCTTCCGTACTCTGCGTTGATAC20480.3008022372166393No Hit
GTACTCTGCGTTGATACCACTGCTT20250.29742408709164775No Hit
CCCGTACTCTGCGTTGATACCACTG19870.2918427955807921No Hit
CATGGAAGCAGTGGTATCAACGCAG19080.2802395842819081No Hit
GTATCAACGCAGAGTACATGGGAAG19080.2802395842819081No Hit
AAAAAGTACTCTGCGTTGATACCAC18950.2783301950808259No Hit
TTCCCGTACTCTGCGTTGATACCAC18290.26863637298302406No Hit
GCAGAGTACGGAAGCAGTGGTATCA18060.26525822285803247No Hit
GCTTCCCATGTACTCTGCGTTGATA17650.2592363030700041No Hit
TATCAACGCAGAGTACATGGGAAGC16110.23661738484179978No Hit
GCTTCCATGTACTCTGCGTTGATAC15790.23191734988528978No Hit
CTTCCCGTACTCTGCGTTGATACCA15690.23044858896138037No Hit
CAGTGGTATCAACGCAGAGTACATG15520.22795169539073448No Hit
GCAGTGGTATCAACGCAGAGTACGG15380.22589543009726137No Hit
ACGCAGAGTACATGGGAAGCAGTGG14760.21678911236902323No Hit
CACTGCTTCCGTACTCTGCGTTGAT14760.21678911236902323No Hit
GCGTTGATACCACTGCTTCCATGTA14170.20812342291795793No Hit
GAGTACGGGAAGCAGTGGTATCAAC12760.18741389389083582No Hit
AAAGTACTCTGCGTTGATACCACTG12740.18712014170605393No Hit
GTATCAACGCAGAGTACATGGAAGC12630.18550450468975363No Hit
TGATACCACTGCTTCCGTACTCTGC12470.18315448721149863No Hit
TACCACTGCTTCCGTACTCTGCGTT12370.18168572628758925No Hit
CTGCGTTGATACCACTGCTTCCGTA11750.17257940855935117No Hit
CCATGTACTCTGCGTTGATACCACT11270.1655293561245862No Hit
TATCAACGCAGAGTACATGGAAGCA10870.15965431242894867No Hit
TACGGAAGCAGTGGTATCAACGCAG10790.1584793036898212No Hit
TCAACGCAGAGTACGGAAGCAGTGG10690.1570105427659118No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10510.15436677310287494No Hit
GTATCAACGCAGAGTACGGGAAGCA10400.15275113608657465No Hit
ACGCAGAGTACATGGAAGCAGTGGT9890.14526045537463686No Hit
GCGTTGATACCACTGCTTCCCGTAC9890.14526045537463686No Hit
GTATCAACGCAGAGTACTTTTTTTT9810.14408544663550935No Hit
CCCCATGTACTCTGCGTTGATACCA9480.13923853558660843No Hit
GGTATCAACGCAGAGTACATGGGAA9230.135566633276835No Hit
ATACCACTGCTTCCCATGTACTCTG8750.12851658084207002No Hit
GCTTCCCGTACTCTGCGTTGATACC8670.1273415721029425No Hit
AGCAGTGGTATCAACGCAGAGTACG8200.12043839576056847No Hit
TATCAACGCAGAGTACGGGAAGCAG8120.11926338702144097No Hit
TATCAACGCAGAGTACTTTTTTTTT8060.11838213046709535No Hit
GGGAAGCAGTGGTATCAACGCAGAG8040.11808837828231349No Hit
CTGCTTCCCATGTACTCTGCGTTGA7870.11559148471166755No Hit
ACGGAAGCAGTGGTATCAACGCAGA7790.11441647597254005No Hit
CTCTGCGTTGATACCACTGCTTCCG7760.11397584769536724No Hit
ATACCACTGCTTCCATGTACTCTGC7340.10780705181494789No Hit
TACCACTGCTTCCCATGTACTCTGC7040.10340076904321975No Hit
TCCCATGTACTCTGCGTTGATACCA6880.10105075156496476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA250.00605430918.98604416
ACGAACG250.00605430918.98604415
TAGCGTA350.002167448916.2869115
ATACCGT702.4978362E-816.2845176
TACCGTC702.5092959E-816.2785367
TAACGAA456.7931594E-414.76692413
CATGGCA400.005223821414.270984
ATAGCGT400.005223821414.270984
AATAGCG400.005223821414.270983
TATACAG400.005272492814.2510485
ATTACCG1101.8735591E-1013.80803315
GGTCCAA707.292354E-613.56345311
CGGTCCA707.292354E-613.56345310
GTCGTAG852.7108217E-713.40388311
CGTCGTA852.7108217E-713.40388310
ACCGTCG852.7108217E-713.4038838
TGTAGGA500.001480694713.320562
AGCGTAT500.001498865913.2990246
AGCTCTA500.001498865913.2990246
GTAAACG500.001506354513.29023217
Produced by FastQC (version 0.11.5)