##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680846
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.195193920504785	31.0	30.0	34.0	26.0	34.0
2	30.084959006882613	31.0	30.0	34.0	26.0	34.0
3	30.42579526060225	31.0	30.0	34.0	26.0	34.0
4	34.045124741865266	37.0	35.0	37.0	30.0	37.0
5	33.910283676484845	35.0	35.0	37.0	30.0	37.0
6	33.979722286684506	37.0	35.0	37.0	30.0	37.0
7	33.86414108329931	37.0	35.0	37.0	30.0	37.0
8	33.87923994559709	37.0	35.0	37.0	30.0	37.0
9	35.428196684712844	39.0	35.0	39.0	30.0	39.0
10	35.35274202976885	39.0	35.0	39.0	30.0	39.0
11	35.34821971488413	39.0	35.0	39.0	30.0	39.0
12	35.289649935521396	39.0	35.0	39.0	30.0	39.0
13	35.16798071810659	38.0	35.0	39.0	30.0	39.0
14	36.28649797457869	39.0	36.0	41.0	30.0	41.0
15	36.209740528695185	39.0	36.0	41.0	30.0	41.0
16	36.03230686528231	39.0	36.0	40.0	29.0	41.0
17	36.082011203708326	39.0	36.0	40.0	29.0	41.0
18	35.95665099009174	39.0	36.0	40.0	29.0	41.0
19	36.116823775126825	39.0	36.0	41.0	29.0	41.0
20	36.08166310736936	39.0	36.0	41.0	29.0	41.0
21	36.05237601454661	39.0	36.0	41.0	29.0	41.0
22	36.019848835125714	39.0	36.0	41.0	29.0	41.0
23	35.922539017633945	39.0	36.0	41.0	29.0	41.0
24	35.81397702270411	39.0	36.0	41.0	28.0	41.0
25	35.5493650546526	39.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8361831718082229
1101	2	1.1784827522096855
1101	3	0.9529814461005124
1101	4	0.9356739629931852
1101	5	0.9976958287350044
1101	6	0.886760849345869
1101	7	0.8810939392961359
1101	8	0.8913318692172894
1101	9	0.8758219152003406
1101	10	1.0355078837339207
1101	11	1.0080061976859724
1101	12	0.967918959071568
1101	13	1.133149925533779
1101	14	1.1312163361795768
1101	15	1.1955614507500982
1101	16	1.095993905347541
1101	17	1.2644144498057983
1101	18	1.186197810104467
1101	19	1.276722201687413
1101	20	1.341382609639247
1101	21	1.1135521397688066
1101	22	1.2082225340353006
1101	23	1.3873078257660438
1101	24	1.3319239170460548
1101	25	1.2105220634209672
1102	1	0.8296680458160921
1102	2	1.2772954964236938
1102	3	1.0297666236521472
1102	4	0.928860743150203
1102	5	0.9433692565995955
1102	6	0.9077256825289197
1102	7	0.7981866902482224
1102	8	0.7867273514026749
1102	9	1.0844785275990674
1102	10	1.0325913533151336
1102	11	0.9946621627008199
1102	12	0.866695040219156
1102	13	0.9946048217046339
1102	14	1.2184430559581472
1102	15	1.17214415569169
1102	16	1.157506933948035
1102	17	1.3087627225129594
1102	18	1.0855287917167828
1102	19	1.0589419356654872
1102	20	1.007551352228191
1102	21	1.186815516007833
1102	22	1.2228638140057058
1102	23	1.2198624818103312
1102	24	1.0799916743563713
1102	25	1.0395384032578647
1103	1	0.7099481455731969
1103	2	1.111365635092568
1103	3	0.9934576365767036
1103	4	0.8055729828922011
1103	5	0.7733467543859334
1103	6	0.8052293178143373
1103	7	0.9012850094872107
1103	8	0.9826347105201307
1103	9	1.037305926684354
1103	10	1.0440812923073324
1103	11	0.9719503616301353
1103	12	0.9891891303417424
1103	13	1.1495443419281912
1103	14	1.1532735582367977
1103	15	1.2791620843507303
1103	16	1.2682738776275144
1103	17	1.270735606126955
1103	18	1.270323144229799
1103	19	1.128321053286264
1103	20	1.0923132105698485
1103	21	1.330005956222621
1103	22	1.3903777661905323
1103	23	1.3388423273005472
1103	24	1.4937643288864635
1103	25	1.623867276766184
1104	1	0.7109961990713423
1104	2	1.037073193830981
1104	3	0.7921267230860813
1104	4	0.8389749874375809
1104	5	0.8935279155834266
1104	6	0.8726955135926957
1104	7	0.7110052560495603
1104	8	0.5605928864819347
1104	9	0.8599151057363628
1104	10	0.6539372462649169
1104	11	0.8253978199777734
1104	12	0.8950261833657223
1104	13	0.7914468615076657
1104	14	0.8911898774598299
1104	15	0.9947128438216382
1104	16	1.1378525306907363
1104	17	0.9870482890458092
1104	18	1.0629769595696388
1104	19	0.803882276941863
1104	20	1.0587313528500601
1104	21	1.0810172215714573
1104	22	1.0090857663867325
1104	23	1.0515563117023703
1104	24	1.1807053533955454
1104	25	1.3052050573780107
1105	1	0.6505161083434885
1105	2	0.9939855871843228
1105	3	0.7455913280120967
1105	4	0.7114969910543465
1105	5	0.47443477167806236
1105	6	0.6013982710110426
1105	7	0.49067825232718576
1105	8	0.5831976727661754
1105	9	0.46774379517056275
1105	10	0.713012712536873
1105	11	0.4658521416554606
1105	12	0.7321807343002149
1105	13	0.7491822071882623
1105	14	0.7093068520498562
1105	15	0.731069139122603
1105	16	0.6567105685709151
1105	17	0.792486273535637
1105	18	0.7891759271828533
1105	19	0.787757123196819
1105	20	0.716311694281849
1105	21	0.48630621350766035
1105	22	0.354040085627247
1105	23	0.6912510475696081
1105	24	0.777665060414769
1105	25	0.695617540906774
1106	1	0.4365716524282277
1106	2	0.8796552069440295
1106	3	0.6243866956446773
1106	4	0.6894201426075739
1106	5	0.4623811137675702
1106	6	0.4315070648400834
1106	7	0.32725871248673855
1106	8	0.5048042531705264
1106	9	0.586649303899506
1106	10	0.5887388617200173
1106	11	0.4782406233968004
1106	12	0.7149213630196556
1106	13	0.6275167934536299
1106	14	0.6790539245336404
1106	15	0.4874234178124226
1106	16	0.5914502151404477
1106	17	0.7415842820513205
1106	18	0.7050339012914009
1106	19	0.6334312391622561
1106	20	0.60879737059809
1106	21	0.7123537537084417
1106	22	0.689619661692447
1106	23	0.7781410204727948
1106	24	0.8386978643809968
1106	25	0.6882211230434478
1107	1	0.4241343317593831
1107	2	0.8035938633207991
1107	3	0.752568747687814
1107	4	0.7070024123216285
1107	5	0.7588362438754217
1107	6	0.7412052937903155
1107	7	0.48798771218991277
1107	8	0.7878959717813885
1107	9	0.6793359567143824
1107	10	0.5188680790941191
1107	11	0.40856297424274857
1107	12	0.47157952073212783
1107	13	0.22280560318945675
1107	14	0.3807450297082724
1107	15	0.4758227450113921
1107	16	0.3950366403902734
1107	17	0.23999686738822135
1107	18	0.14503785894451227
1107	19	0.2173180502832608
1107	20	0.5643252225818642
1107	21	0.7157597099763748
1107	22	0.48601740583017516
1107	23	0.4874909759491999
1107	24	0.027193758315895877
1107	25	0.41614355304245976
1108	1	0.05040020477525786
1108	2	0.5442565617334978
1108	3	0.22857668419575106
1108	4	0.1767682853375021
1108	5	0.054998942288115416
1108	6	0.26890370648872874
1108	7	-0.028750383048183892
1108	8	0.19300311463853603
1108	9	0.035391512269939085
1108	10	0.031959348935387766
1108	11	0.13634868852845727
1108	12	0.28245650485911256
1108	13	0.07122226985612201
1108	14	0.2803005852638236
1108	15	0.330794967233615
1108	16	0.1784572753109117
1108	17	0.3308063911977399
1108	18	0.06708150973816629
1108	19	0.3197624947277049
1108	20	0.2833926828993185
1108	21	0.3612398687065337
1108	22	0.25710073916350495
1108	23	0.18481240452062764
1108	24	0.05513820276033954
1108	25	8.379974869043849E-4
1109	1	0.029661745636143877
1109	2	0.5344507289200209
1109	3	0.17045909218078137
1109	4	0.40084423667873637
1109	5	0.3921909260685297
1109	6	0.33501412445503576
1109	7	0.17705903678962187
1109	8	0.04826278400846462
1109	9	0.08533692711709762
1109	10	0.07637996652740497
1109	11	0.18281624935191587
1109	12	0.23513507628768338
1109	13	0.24908799038013996
1109	14	0.1807372664865312
1109	15	0.11313667029038754
1109	16	0.040692147425694714
1109	17	-0.12928406419838723
1109	18	0.043941303874163395
1109	19	0.015614023111986342
1109	20	0.034698827627956064
1109	21	0.006806774508156366
1109	22	-0.10651825646968405
1109	23	0.2708970894863114
1109	24	0.2977692071271534
1109	25	0.22362729879694854
1110	1	0.3190509984260501
1110	2	0.6324758077652426
1110	3	0.5583881921322593
1110	4	0.6098288012105186
1110	5	0.4084959660976395
1110	6	0.5499552937903118
1110	7	0.462147434412131
1110	8	0.341118194003613
1110	9	0.3661206789366034
1110	10	0.4565625235385582
1110	11	0.37397269646496767
1110	12	0.48704479850990623
1110	13	0.6910139365227863
1110	14	0.614675585263825
1110	15	0.4846144116780593
1110	16	0.5575991403902734
1110	17	0.6828649229437715
1110	18	0.3400725811667371
1110	19	0.48208888361659064
1110	20	0.4803668892485291
1110	21	0.5613638766430427
1110	22	0.514913239163505
1110	23	0.6694354203936399
1110	24	0.722672924982561
1110	25	0.42844216415356584
1111	1	0.24767220180936533
1111	2	0.5172947475960754
1111	3	0.22506086610381004
1111	4	0.32944685676607577
1111	5	0.15005269908495222
1111	6	0.2371068701071124
1111	7	0.12745945382774693
1111	8	0.1868025461758549
1111	9	0.2523032769758231
1111	10	0.3340014893209542
1111	11	0.2620716247617807
1111	12	0.3602215814779939
1111	13	0.29667642210525713
1111	14	0.2514078516998097
1111	15	0.0900726488945196
1111	16	0.1970497122856969
1111	17	0.428248309141388
1111	18	0.19860956213559433
1111	19	0.25315349818791333
1111	20	0.291310997283901
1111	21	0.0036366803723808516
1111	22	0.16335073916350495
1111	23	0.23385775795611607
1111	24	0.26642713009597685
1111	25	0.43092017741769695
1112	1	-0.17139366135000245
1112	2	0.019352691916836307
1112	3	-0.07596527470667169
1112	4	-0.11865822505993862
1112	5	-0.05206470358366744
1112	6	-0.06134423248014542
1112	7	-0.13821728350345808
1112	8	-0.22035898084565275
1112	9	-0.09622993475848318
1112	10	-0.1911818778395613
1112	11	-0.2671166123205637
1112	12	-0.06408972991386719
1112	13	-0.10090843643155978
1112	14	-0.16190441042954262
1112	15	-0.13700757367939076
1112	16	-0.2538040142178204
1112	17	-0.08049236603124399
1112	18	-0.18378212382895498
1112	19	-0.22308849579770396
1112	20	-0.10747494968342863
1112	21	-0.01649868795644238
1112	22	-0.12174831337740954
1112	23	0.02737039024721355
1112	24	-0.008761452709080686
1112	25	0.10367499145761627
1113	1	0.12216000991364595
1113	2	-0.07600609806543801
1113	3	0.1499013087458323
1113	4	0.4313147976870155
1113	5	0.3252553963032625
1113	6	0.3192108927863586
1113	7	0.38414989000630584
1113	8	0.21175001174663777
1113	9	0.2719792620527386
1113	10	0.43281807426739505
1113	11	0.42313812628444936
1113	12	0.43287617098794584
1113	13	0.5552307407509929
1113	14	0.41461856962524735
1113	15	0.4885436640190193
1113	16	0.48935308020107016
1113	17	0.4270736122477601
1113	18	0.42272489437264227
1113	19	0.43900663026202125
1113	20	0.2635008186818695
1113	21	0.30457252974662197
1113	22	0.4109615124041639
1113	23	0.318768126253282
1113	24	0.23644793342932502
1113	25	0.4795141486597956
1114	1	-0.40277277206575235
1114	2	-0.7727922933276545
1114	3	-0.4735733288149149
1114	4	-0.4833946732885721
1114	5	-0.30437687087867715
1114	6	-0.4233484402716172
1114	7	-0.1561215774275766
1114	8	-0.27067597721678993
1114	9	-0.19969500867723866
1114	10	-0.41394066407528385
1114	11	-0.36662099661335645
1114	12	-0.16122193646277339
1114	13	-0.2839120652987077
1114	14	-0.378351509453843
1114	15	-0.3940570364093716
1114	16	-0.5296190973146437
1114	17	-0.36035285938773853
1114	18	-0.627894745791366
1114	19	-0.5996364123761211
1114	20	-0.35760556066039584
1114	21	-0.28345340477772396
1114	22	-0.383097348268187
1114	23	-0.40012286740235226
1114	24	-0.4511231815748147
1114	25	-0.36998395151127994
1115	1	-1.2204020340307125
1115	2	-1.6532514340021116
1115	3	-1.1548721430963589
1115	4	-1.0570136976049298
1115	5	-0.9799024436393893
1115	6	-1.1254992785858988
1115	7	-0.857561683687841
1115	8	-0.9341424077495262
1115	9	-1.207812112463536
1115	10	-0.9982245529835865
1115	11	-1.3518975801282593
1115	12	-1.1136922158231926
1115	13	-1.2783912695895054
1115	14	-1.3638358752905475
1115	15	-1.3833708110179828
1115	16	-1.5191173515547618
1115	17	-1.4567735661583328
1115	18	-1.2415672109441402
1115	19	-1.5794912366155813
1115	20	-1.7490033725377785
1115	21	-1.4378939266026336
1115	22	-1.6223635465507797
1115	23	-1.95877768076722
1115	24	-1.818928961418763
1115	25	-1.618738228525892
1116	1	-3.366122254724008
1116	2	-4.381654704834986
1116	3	-3.2190853187497552
1116	4	-2.9978727308627917
1116	5	-2.6810927366515003
1116	6	-3.4328267795201093
1116	7	-3.6802047276726313
1116	8	-3.3463102137856175
1116	9	-3.6946342609259375
1116	10	-4.249658839577133
1116	11	-4.163890634004677
1116	12	-4.304040181444275
1116	13	-3.8008782456077945
1116	14	-4.367122095148545
1116	15	-4.099810702869476
1116	16	-4.419536097295865
1116	17	-4.401336465945114
1116	18	-5.217997650792025
1116	19	-4.564258123828996
1116	20	-4.981528156570487
1116	21	-4.508393783723509
1116	22	-4.7026286422797945
1116	23	-4.934158508586886
1116	24	-4.529269013734506
1116	25	-4.7574738581832
1201	1	0.9133555457138094
1201	2	1.3220096729598367
1201	3	1.0471176486759184
1201	4	0.8689205409766032
1201	5	1.0986095852748932
1201	6	1.0723325371861563
1201	7	1.1465769438898548
1201	8	0.9934422512732226
1201	9	1.0504777191664942
1201	10	1.1512149232159175
1201	11	1.0713143354042742
1201	12	0.9186196497904078
1201	13	1.1997953188579373
1201	14	1.3421880625777973
1201	15	1.2388144772154703
1201	16	1.1541864689847472
1201	17	1.2574657119133263
1201	18	1.514882585703944
1201	19	1.43455378329395
1201	20	1.3536496831585936
1201	21	1.3505272034226365
1201	22	1.5217899406154132
1201	23	1.397291338925605
1201	24	1.427529309838377
1201	25	1.3532994312437054
1202	1	0.6099121095371629
1202	2	0.8151494188763522
1202	3	0.597013192132259
1202	4	0.7096690789882985
1202	5	0.5695029105420844
1202	6	0.49142751601253565
1202	7	0.36976548996769054
1202	8	0.5870070828925051
1202	9	0.580669290047716
1202	10	0.47264585687189253
1202	11	0.5978963075760788
1202	12	0.5026906318432438
1202	13	0.5250278254116765
1202	14	0.593189474152716
1202	15	0.7069338561225038
1202	16	0.5870366403902736
1202	17	0.7902190896104386
1202	18	0.7245934145000703
1202	19	0.8297624947277029
1202	20	0.7678807781374175
1202	21	0.9122041544208201
1202	22	0.5713507391635062
1202	23	0.7266020870603072
1202	24	0.6094159805381167
1202	25	0.8365879974869017
1203	1	0.867586075473902
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2211	11	-0.13655884871956658
2211	12	-0.2270910411531588
2211	13	-0.06985463636649314
2211	14	-0.3922673376108463
2211	15	-0.3613555586209003
2211	16	-0.20932756255061236
2211	17	-0.09327336340348324
2211	18	-0.17924298631792368
2211	19	-0.10815161570174325
2211	20	-0.08820569571592785
2211	21	-0.23814115670976577
2211	22	-0.1153521530363193
2211	23	-0.10076493991407887
2211	24	-0.04165189364632482
2211	25	-0.08021480707050443
2212	1	-0.01771758581924132
2212	2	-0.7433598978938321
2212	3	-0.25546280116360975
2212	4	-0.030579762577310987
2212	5	-0.11168805859919217
2212	6	-0.21993835207383228
2212	7	-0.1699549083365568
2212	8	-0.29558938757974573
2212	9	-0.22209517805836754
2212	10	-0.23398452861563612
2212	11	-0.2544787293952311
2212	12	-0.19503351288711457
2212	13	-0.14675133262834805
2212	14	-0.04427794179917299
2212	15	-0.04150427855165617
2212	16	0.07509658320797996
2212	17	-1.0850099353328702
2212	18	-0.41208330836298757
2212	19	-0.1289952692474472
2212	20	-0.15345847060555684
2212	21	-0.1832421632366774
2212	22	0.07018303730015418
2212	23	0.12422566982674965
2212	24	0.11297981766717413
2212	25	-0.1300348951484125
2213	1	-0.07705790809952262
2213	2	-0.36340437301077344
2213	3	0.09716134028040813
2213	4	0.05651916717172156
2213	5	0.12050820154738062
2213	6	0.1923164049014261
2213	7	-0.18772304618751434
2213	8	0.142485037037126
2213	9	0.2899109126226733
2213	10	0.1400391549318627
2213	11	0.1231873128670884
2213	12	0.2398969810495899
2213	13	0.14325710230762212
2213	14	-0.017406645776738117
2213	15	-0.01590917738719355
2213	16	0.22433470035500136
2213	17	-0.874881439490089
2213	18	-0.5131984726251488
2213	19	-0.3685091102105673
2213	20	-0.037297352374046966
2213	21	0.09910362532028927
2213	22	0.1333683758478088
2213	23	0.38272907118729194
2213	24	0.3870385554940228
2213	25	0.2698084560406926
2214	1	-0.6880708588326669
2214	2	-2.0617848876929905
2214	3	-1.1044134096520075
2214	4	-0.8384363549127585
2214	5	-0.8217541859704838
2214	6	-0.6807573988293143
2214	7	-0.6832993924329571
2214	8	-0.7214324953718929
2214	9	-0.8355026483140051
2214	10	-0.6652187011167499
2214	11	-0.6214589235674737
2214	12	-0.642054704734214
2214	13	-0.7907759053263632
2214	14	-0.7976191227653686
2214	15	-0.6866776605036122
2214	16	-0.7962950708830405
2214	17	-0.7033437652151875
2214	18	-0.5390902837967673
2214	19	-0.5315148775350664
2214	20	-0.581968370281075
2214	21	-0.5880367214915907
2214	22	-0.6961383119313851
2214	23	-0.6960856663865727
2214	24	-0.6405734760717792
2214	25	-0.6683682068926586
2215	1	-1.4656022392487102
2215	2	-2.3209653714106224
2215	3	-1.6760787872643554
2215	4	-1.5220983970970607
2215	5	-1.5001512469266416
2215	6	-1.2009874950249895
2215	7	-1.330007872799051
2215	8	-1.5861261032737985
2215	9	-1.4294263685247657
2215	10	-1.6826712880876329
2215	11	-1.7562758778543852
2215	12	-1.6516198905997328
2215	13	-1.4938301583999518
2215	14	-1.7808473176059323
2215	15	-2.023606246894417
2215	16	-1.7278301734434294
2215	17	-1.6728596447530606
2215	18	-1.733137269826642
2215	19	-2.0108114567071738
2215	20	-1.9939823565204193
2215	21	-1.6631865740559988
2215	22	-1.5651260820285486
2215	23	-2.021559796677735
2215	24	-2.086004181345622
2215	25	-2.1810190665307587
2216	1	-4.521562215963272
2216	2	-5.382657369635396
2216	3	-4.813679003980301
2216	4	-4.776950010074657
2216	5	-4.839049453832732
2216	6	-4.322769176763792
2216	7	-4.1830444897247965
2216	8	-4.100751547800854
2216	9	-4.391990994257476
2216	10	-4.396343989371214
2216	11	-5.014392639334279
2216	12	-4.645282678281507
2216	13	-5.059081254948055
2216	14	-5.17452970194244
2216	15	-5.429599941382573
2216	16	-5.16533701786328
2216	17	-5.157211139574358
2216	18	-5.416760806561722
2216	19	-5.071229672374123
2216	20	-5.397647507328205
2216	21	-5.548036054456464
2216	22	-5.3640644044396275
2216	23	-6.070211373920255
2216	24	-6.214607228049061
2216	25	-6.156978582147563
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13217.0
3	0.0
4	227.0
5	1765.0
6	2363.0
7	1566.0
8	1164.0
9	1034.0
10	928.0
11	836.0
12	808.0
13	789.0
14	766.0
15	845.0
16	811.0
17	874.0
18	960.0
19	1285.0
20	1604.0
21	2155.0
22	2618.0
23	3127.0
24	3923.0
25	4427.0
26	5259.0
27	6271.0
28	7183.0
29	8729.0
30	11521.0
31	14901.0
32	19625.0
33	26545.0
34	36315.0
35	51611.0
36	79362.0
37	142725.0
38	222707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.47020844455622	15.61532544735499	14.415368392257562	23.499097715831226
2	11.072871223137826	22.602858443866683	37.41109593266646	28.913174400329027
3	21.7328147628098	28.86541156149849	27.459072976855264	21.942700698836447
4	14.485883019173206	15.336652668583742	31.74941715512867	38.428047157114385
5	17.2286361238581	32.54779568968178	29.860275393687193	20.363292792772935
6	37.93843761985443	30.43968300385151	12.67999288771853	18.94188648857553
7	25.360036201967013	32.10118332241201	22.389094745131686	20.149685730489285
8	25.653463470242492	34.433591540709166	23.946444861976822	15.966500127071523
9	27.736026441424897	20.49283878075652	15.251413881748071	36.51972089607051
10	13.429389394271007	25.30218880318692	30.82009979685157	30.44832200569051
11	31.908823957141326	25.316085664466637	17.769985706919876	25.005104671472157
12	26.186527447054488	23.24872529735717	25.697946520513494	24.86680073507485
13	32.75499157604983	19.014331731779077	27.337517644760478	20.89315904741061
14	31.733387779563277	18.545110562078985	23.153406169854613	26.56809548850313
15	34.00299924945325	18.828882684107946	25.926763674712156	21.241354391726656
16	28.28657288138581	20.771363862018724	30.891420379939078	20.050642876656394
17	24.579567185530944	29.0807319129436	27.757084568316476	18.58261633320898
18	27.098785922220298	17.915211369384558	37.38716244202066	17.59884026637448
19	24.51038854601481	34.12372254518643	18.646654309491424	22.719234599307335
20	19.490751212462143	28.47016799687448	29.455265948540493	22.583814842122887
21	21.154134267106528	33.0760463087268	20.531651444871052	25.238167979295618
22	19.997649858257077	30.461509084766675	18.134868759272045	31.405972297704203
23	24.299657043820545	23.771930468755738	19.222583701136088	32.705828786287626
24	18.671200282004584	28.510222666118324	20.447829152223722	32.37074789965337
25	27.37392792604693	27.613449060221406	19.766154443344945	25.24646857038672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	317.0
1	317.0
2	400.5
3	484.0
4	484.0
5	484.0
6	957.5
7	1431.0
8	1431.0
9	1431.0
10	1329.0
11	1227.0
12	1227.0
13	1227.0
14	1298.0
15	1369.0
16	1369.0
17	1369.0
18	2284.5
19	3200.0
20	3200.0
21	3200.0
22	4915.0
23	6630.0
24	6630.0
25	6630.0
26	9440.5
27	12251.0
28	12251.0
29	12251.0
30	14437.0
31	16623.0
32	16623.0
33	16623.0
34	19881.5
35	23140.0
36	23140.0
37	23140.0
38	26499.5
39	29859.0
40	29859.0
41	29859.0
42	36087.0
43	42315.0
44	42315.0
45	42315.0
46	82279.0
47	122243.0
48	122243.0
49	122243.0
50	195700.5
51	269158.0
52	269158.0
53	269158.0
54	168007.0
55	66856.0
56	66856.0
57	66856.0
58	48342.5
59	29829.0
60	29829.0
61	29829.0
62	25525.0
63	21221.0
64	21221.0
65	21221.0
66	17509.0
67	13797.0
68	13797.0
69	13797.0
70	10727.0
71	7657.0
72	7657.0
73	7657.0
74	6220.0
75	4783.0
76	4783.0
77	4783.0
78	4167.5
79	3552.0
80	3552.0
81	3552.0
82	2652.5
83	1753.0
84	1753.0
85	1753.0
86	1286.0
87	819.0
88	819.0
89	819.0
90	553.0
91	287.0
92	287.0
93	287.0
94	163.5
95	40.0
96	40.0
97	40.0
98	22.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13292286361379813
2	0.00822506117389248
3	0.0
4	0.14555420755941872
5	0.04303469507054459
6	0.04890973876618207
7	0.03231274032600617
8	0.01865326373364902
9	0.014100104869529966
10	0.00925319382062904
11	0.014246980961920905
12	0.014540733146702777
13	0.007196928527155921
14	0.007637556804328732
15	0.0013218848315184344
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0035250262173824915
22	0.005287539326073737
23	1.4687609239093715E-4
24	8.812565543456229E-4
25	0.10854143227690255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	680846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.74436124003104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.92945710345981	30.683564478590547
2	5.401674948237226	3.645521415090859
3	1.1428912485433316	1.1569840544674888
4	0.5025462224830901	0.6783240508512961
5	0.3314678601749284	0.5592585606601441
6	0.224912173674169	0.4553710581445054
7	0.14796098512702405	0.3494994252090012
8	0.11612500570310985	0.3134851313157123
9	0.08886618230187308	0.26988593018551726
>10	0.8350322222034862	5.80134174988876
>50	0.1327425629071869	3.1791295693175674
>100	0.10777118968699835	7.200458680898605
>500	0.012704733743589236	3.1519284607025515
>1k	0.018838053481873698	12.64245438913848
>5k	0.004819036937223503	11.908911449219804
>10k+	0.0021904713351015926	18.003881596319165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	38809	5.70011426959988	No Hit
CTTCCGTACTCTGCGTTGATACCAC	24391	3.5824547695073483	No Hit
GAGTACGGAAGCAGTGGTATCAACG	21855	3.2099769992039318	No Hit
TCCGTACTCTGCGTTGATACCACTG	20948	3.0767603834053516	No Hit
GCTTCCGTACTCTGCGTTGATACCA	15783	2.3181453662061613	No Hit
GTATCAACGCAGAGTACGGAAGCAG	9766	1.4343919182898923	No Hit
GCGTTGATACCACTGCTTCCGTACT	9691	1.423376211360572	No Hit
CCCATGTACTCTGCGTTGATACCAC	9317	1.3684445528063616	No Hit
TATCAACGCAGAGTACGGAAGCAGT	8474	1.2446280069208016	No Hit
TCCATGTACTCTGCGTTGATACCAC	7692	1.1297709026710887	No Hit
ACTCTGCGTTGATACCACTGCTTCC	6461	0.948966432937845	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6414	0.942063256595471	No Hit
GTACATGGAAGCAGTGGTATCAACG	6346	0.9320756823128872	No Hit
CAGTGGTATCAACGCAGAGTACGGA	5812	0.8536438489761269	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	5473	0.803852853655599	No Hit
GGTATCAACGCAGAGTACGGAAGCA	5111	0.7506837082100798	No Hit
ATACCACTGCTTCCGTACTCTGCGT	4733	0.6951645452863056	No Hit
CATGTACTCTGCGTTGATACCACTG	4052	0.5951419263680774	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4034	0.5924981567050405	No Hit
GTGGTATCAACGCAGAGTACGGAAG	3756	0.55166660302036	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	3719	0.5462321876018953	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	3018	0.4432720468358483	No Hit
GATACCACTGCTTCCGTACTCTGCG	2677	0.3931872993305388	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2458	0.3610214350969235	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2435	0.357643284971932	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2387	0.350593232537167	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2341	0.34383693228718387	No Hit
GTTGATACCACTGCTTCCGTACTCT	2328	0.3419275430861017	No Hit
GCGTTGATACCACTGCTTCCCATGT	2156	0.3166648551948605	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2111	0.31005543103726835	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2048	0.3008022372166393	No Hit
GTACTCTGCGTTGATACCACTGCTT	2025	0.29742408709164775	No Hit
CCCGTACTCTGCGTTGATACCACTG	1987	0.2918427955807921	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1908	0.2802395842819081	No Hit
GTATCAACGCAGAGTACATGGGAAG	1908	0.2802395842819081	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1895	0.2783301950808259	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1829	0.26863637298302406	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1806	0.26525822285803247	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1765	0.2592363030700041	No Hit
TATCAACGCAGAGTACATGGGAAGC	1611	0.23661738484179978	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1579	0.23191734988528978	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1569	0.23044858896138037	No Hit
CAGTGGTATCAACGCAGAGTACATG	1552	0.22795169539073448	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1538	0.22589543009726137	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1476	0.21678911236902323	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1476	0.21678911236902323	No Hit
GCGTTGATACCACTGCTTCCATGTA	1417	0.20812342291795793	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1276	0.18741389389083582	No Hit
AAAGTACTCTGCGTTGATACCACTG	1274	0.18712014170605393	No Hit
GTATCAACGCAGAGTACATGGAAGC	1263	0.18550450468975363	No Hit
TGATACCACTGCTTCCGTACTCTGC	1247	0.18315448721149863	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1237	0.18168572628758925	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1175	0.17257940855935117	No Hit
CCATGTACTCTGCGTTGATACCACT	1127	0.1655293561245862	No Hit
TATCAACGCAGAGTACATGGAAGCA	1087	0.15965431242894867	No Hit
TACGGAAGCAGTGGTATCAACGCAG	1079	0.1584793036898212	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	1069	0.1570105427659118	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1051	0.15436677310287494	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1040	0.15275113608657465	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	989	0.14526045537463686	No Hit
GCGTTGATACCACTGCTTCCCGTAC	989	0.14526045537463686	No Hit
GTATCAACGCAGAGTACTTTTTTTT	981	0.14408544663550935	No Hit
CCCCATGTACTCTGCGTTGATACCA	948	0.13923853558660843	No Hit
GGTATCAACGCAGAGTACATGGGAA	923	0.135566633276835	No Hit
ATACCACTGCTTCCCATGTACTCTG	875	0.12851658084207002	No Hit
GCTTCCCGTACTCTGCGTTGATACC	867	0.1273415721029425	No Hit
AGCAGTGGTATCAACGCAGAGTACG	820	0.12043839576056847	No Hit
TATCAACGCAGAGTACGGGAAGCAG	812	0.11926338702144097	No Hit
TATCAACGCAGAGTACTTTTTTTTT	806	0.11838213046709535	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	804	0.11808837828231349	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	787	0.11559148471166755	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	779	0.11441647597254005	No Hit
CTCTGCGTTGATACCACTGCTTCCG	776	0.11397584769536724	No Hit
ATACCACTGCTTCCATGTACTCTGC	734	0.10780705181494789	No Hit
TACCACTGCTTCCCATGTACTCTGC	704	0.10340076904321975	No Hit
TCCCATGTACTCTGCGTTGATACCA	688	0.10105075156496476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4687609239093715E-4	0.0
11	0.0	0.0	0.0	1.4687609239093715E-4	0.0
12	0.0	0.0	0.0	8.81256554345623E-4	0.0
13	0.0	0.0	0.0	0.0014687609239093715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	25	0.006054309	18.986044	16
ACGAACG	25	0.006054309	18.986044	15
TAGCGTA	35	0.0021674489	16.286911	5
ATACCGT	70	2.4978362E-8	16.284517	6
TACCGTC	70	2.5092959E-8	16.278536	7
TAACGAA	45	6.7931594E-4	14.766924	13
CATGGCA	40	0.0052238214	14.27098	4
ATAGCGT	40	0.0052238214	14.27098	4
AATAGCG	40	0.0052238214	14.27098	3
TATACAG	40	0.0052724928	14.251048	5
ATTACCG	110	1.8735591E-10	13.808033	15
GGTCCAA	70	7.292354E-6	13.563453	11
CGGTCCA	70	7.292354E-6	13.563453	10
GTCGTAG	85	2.7108217E-7	13.403883	11
CGTCGTA	85	2.7108217E-7	13.403883	10
ACCGTCG	85	2.7108217E-7	13.403883	8
TGTAGGA	50	0.0014806947	13.32056	2
AGCGTAT	50	0.0014988659	13.299024	6
AGCTCTA	50	0.0014988659	13.299024	6
GTAAACG	50	0.0015063545	13.290232	17
>>END_MODULE