FastQCFastQC Report
Thu 2 Feb 2017
SRR4050253_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050253_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9902810
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT163610.1652157316963569No Hit
GTACAAAGGGCAGGGACTTAATCAA154340.15585475233797275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.016.1305751
ACGCTTA7950.013.99204217
GTATCAA197000.013.8007961
CTCGACG8250.013.71525613
CGCTTAC8250.013.71365218
TCGACGC8700.012.6775814
CGACGCT9050.012.39741215
GACGCTT9050.012.18716316
CGTCGTA14600.011.92076310
AACCGCG8200.011.8295387
TAGATCG5200.011.1560445
GTCGTAG16150.010.54110811
CGAACGA8300.010.53908616
CCGTCGT16950.010.3802519
ACCTCGA12150.010.17590111
ATACCGT17650.010.1298096
AGAACCG10250.010.1131485
CGACCAT15950.010.07701410
AGGCCCG19550.010.02135110
TACCGTC17950.09.9603587
Produced by FastQC (version 0.11.5)