##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050253_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9902810
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.669761007229262	31.0	31.0	34.0	27.0	34.0
2	30.77153050497788	31.0	31.0	34.0	27.0	34.0
3	30.882805082597766	31.0	31.0	34.0	27.0	34.0
4	34.43205686062845	37.0	35.0	37.0	31.0	37.0
5	34.44370719018137	37.0	35.0	37.0	31.0	37.0
6	34.48303198789031	37.0	35.0	37.0	31.0	37.0
7	34.42926139146363	37.0	35.0	37.0	31.0	37.0
8	34.39364251156995	37.0	35.0	37.0	31.0	37.0
9	35.9718594015234	39.0	37.0	39.0	31.0	39.0
10	35.855990269428574	39.0	37.0	39.0	31.0	39.0
11	35.74689800167831	39.0	37.0	39.0	30.0	39.0
12	35.67009929504858	39.0	37.0	39.0	30.0	39.0
13	35.67136711701022	39.0	37.0	39.0	30.0	39.0
14	36.854021737264475	40.0	37.0	41.0	31.0	41.0
15	36.804962732800085	40.0	37.0	41.0	31.0	41.0
16	36.80873065321863	40.0	37.0	41.0	31.0	41.0
17	36.7781730640091	40.0	37.0	41.0	31.0	41.0
18	36.74691850091035	40.0	37.0	41.0	31.0	41.0
19	36.764639329644815	40.0	37.0	41.0	30.0	41.0
20	36.724320874580044	40.0	37.0	41.0	30.0	41.0
21	36.675091615410174	40.0	37.0	41.0	30.0	41.0
22	36.59632104422886	40.0	37.0	41.0	30.0	41.0
23	36.52388645243118	40.0	37.0	41.0	30.0	41.0
24	36.46006598127198	40.0	37.0	41.0	30.0	41.0
25	36.22346495590646	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5961860338764247
1101	2	0.5889016243946621
1101	3	0.5767870025974524
1101	4	0.6158143338697428
1101	5	0.6136169064064347
1101	6	0.6485663478250601
1101	7	0.6425899389341865
1101	8	0.6696619045128642
1101	9	0.7151734285948237
1101	10	0.7279745759249039
1101	11	0.614281981121124
1101	12	0.6309318684876146
1101	13	0.5894182979031157
1101	14	0.7121114594435483
1101	15	0.7552978506053876
1101	16	0.7994158068565227
1101	17	0.78643303769055
1101	18	0.818538402951063
1101	19	0.844946413833533
1101	20	0.8031761289066068
1101	21	0.7744033499705338
1101	22	0.8387911401921286
1101	23	0.8512694544604429
1101	24	0.8704863023520133
1101	25	0.885854023258176
1102	1	0.4836873403154094
1102	2	0.50823697674182
1102	3	0.4634412127409391
1102	4	0.45252323690071705
1102	5	0.3991823913739623
1102	6	0.3841041022872673
1102	7	0.4203061470934486
1102	8	0.5021487140752896
1102	9	0.5104224029992963
1102	10	0.5684816591033766
1102	11	0.5397762860041695
1102	12	0.4421683367389164
1102	13	0.4937236609591693
1102	14	0.5567684627346878
1102	15	0.5932936999196698
1102	16	0.6424037868119825
1102	17	0.5775245719306881
1102	18	0.6474803568659908
1102	19	0.6043471508388905
1102	20	0.6112644406056447
1102	21	0.6167209573630927
1102	22	0.654471722245134
1102	23	0.6327435578554628
1102	24	0.575615647492512
1102	25	0.6649935460053769
1103	1	0.5075970858969541
1103	2	0.48752304425363135
1103	3	0.4930259218776456
1103	4	0.4817512244759854
1103	5	0.4662510673574616
1103	6	0.42850769474382844
1103	7	0.4468442995590749
1103	8	0.461502545805736
1103	9	0.5536486855227736
1103	10	0.5233776303389988
1103	11	0.4565731066749592
1103	12	0.4469648895465852
1103	13	0.4333931590764024
1103	14	0.6099632455852699
1103	15	0.5806764454136442
1103	16	0.5818891290328807
1103	17	0.48327178126056225
1103	18	0.5474172544959188
1103	19	0.5434330164231156
1103	20	0.5882175233495914
1103	21	0.5288390592438006
1103	22	0.5622615166601861
1103	23	0.5813269740374878
1103	24	0.6313358129495015
1103	25	0.6493178870363394
1104	1	0.5211583600314356
1104	2	0.5447991194021817
1104	3	0.5651681420447687
1104	4	0.5137016699086132
1104	5	0.5629127247211372
1104	6	0.5667358076928508
1104	7	0.5509531040221063
1104	8	0.5635997473018222
1104	9	0.6299253321622373
1104	10	0.5897971847362271
1104	11	0.5381614410541928
1104	12	0.5993615482764341
1104	13	0.5526060629608409
1104	14	0.6230582441890675
1104	15	0.5551674081506377
1104	16	0.6481763197296857
1104	17	0.6183257926563499
1104	18	0.7237364626957614
1104	19	0.6921602731366292
1104	20	0.6856060550983685
1104	21	0.7041732963534812
1104	22	0.7107134118381424
1104	23	0.7250161530422545
1104	24	0.7421775259809991
1104	25	0.873971364373368
1105	1	0.35110313325866827
1105	2	0.4005933947925584
1105	3	0.4516477480235821
1105	4	0.5074579576624387
1105	5	0.46460638507497265
1105	6	0.4249616465444035
1105	7	0.4393644356478319
1105	8	0.5008763592673375
1105	9	0.5232565062544481
1105	10	0.5314867924065183
1105	11	0.49357288792157306
1105	12	0.4184122986183212
1105	13	0.4486429147559292
1105	14	0.6094657765359699
1105	15	0.5363917445302349
1105	16	0.47246558166008157
1105	17	0.5364896955578473
1105	18	0.614365421561132
1105	19	0.6051969116581262
1105	20	0.5736542000530349
1105	21	0.5256546955402328
1105	22	0.5850013783731924
1105	23	0.5641831518639648
1105	24	0.57379037270411
1105	25	0.6225100874920955
1106	1	0.2303982904771935
1106	2	0.235572394436673
1106	3	0.273735989490568
1106	4	0.26857415690669484
1106	5	0.30368600392841216
1106	6	0.3009642789697651
1106	7	0.2846536620934401
1106	8	0.27623812074718046
1106	9	0.2604531310334508
1106	10	0.3119766915278106
1106	11	0.2787609097693746
1106	12	0.23311826371658384
1106	13	0.14244801920571604
1106	14	0.25518615391176525
1106	15	0.32507604664614576
1106	16	0.2258031650161243
1106	17	0.2926435426606133
1106	18	0.31167401454437993
1106	19	0.226895776992464
1106	20	0.29731679571605696
1106	21	0.2793445955232059
1106	22	0.2767452779666755
1106	23	0.09989244729128899
1106	24	0.2853441044488534
1106	25	0.25136795248565136
1107	1	0.3756601274857445
1107	2	0.29874361059815513
1107	3	0.2809547942052468
1107	4	0.31813243066174124
1107	5	0.3476952502673498
1107	6	0.2699421193276663
1107	7	0.3428885858455928
1107	8	0.34586404669700244
1107	9	0.4433023154234377
1107	10	0.42637001540933994
1107	11	0.3577032696961808
1107	12	0.294173539704083
1107	13	0.3540419141082154
1107	14	0.36758167838740263
1107	15	0.38434050549052046
1107	16	0.34456583719007483
1107	17	0.3938603372219234
1107	18	0.4367611104683604
1107	19	0.42703275313740363
1107	20	0.42374561053613036
1107	21	0.4229958481134233
1107	22	0.44527795175149976
1107	23	0.40934062092802037
1107	24	0.4061838537468816
1107	25	0.46288319576158443
1108	1	0.2269682676355309
1108	2	0.15908991441182252
1108	3	0.20089687710385817
1108	4	0.21375118795075565
1108	5	0.23297533425778738
1108	6	0.20839357854177365
1108	7	0.18529755927790603
1108	8	0.13191263288301514
1108	9	0.14485594796944667
1108	10	0.11358810327929802
1108	11	0.07924660293941344
1108	12	0.05691932990637838
1108	13	0.08822258716017473
1108	14	0.056567405026861195
1108	15	0.14380837158123683
1108	16	0.13683008021336462
1108	17	0.08096197011652606
1108	18	0.0471036313099944
1108	19	0.12853668379897698
1108	20	0.12450504161454035
1108	21	0.17098883115675534
1108	22	0.23371223284364362
1108	23	0.16173057974906158
1108	24	0.13505918796322192
1108	25	0.04553566166929812
1109	1	-0.013797649755932895
1109	2	-0.01439208005961845
1109	3	0.054888365504201175
1109	4	0.012538125250159737
1109	5	0.05440862709211558
1109	6	-0.0170683946751069
1109	7	0.05775403972656079
1109	8	0.033261373645686376
1109	9	0.02529633927397157
1109	10	-0.09237548550387231
1109	11	-0.03372171879737351
1109	12	-0.011681650100825891
1109	13	-0.10059863607499864
1109	14	-0.045996109415987974
1109	15	-0.002801073584748792
1109	16	-0.04029773677048354
1109	17	-0.05216972223607286
1109	18	-0.0062188160612350885
1109	19	-0.024241514934097097
1109	20	-0.06179446832948088
1109	21	-0.0021559606650427554
1109	22	0.02614703186377909
1109	23	-0.01549291241962436
1109	24	-0.011323115933635108
1109	25	-0.05158778009532483
1110	1	-0.061470543394992916
1110	2	-0.04792932846748954
1110	3	0.0024678199990297855
1110	4	-0.02026114946348656
1110	5	-0.014301435402295226
1110	6	-0.02435981120753894
1110	7	-0.007448021631596191
1110	8	-0.031241352593646354
1110	9	0.02543713013349702
1110	10	-0.07124576199149146
1110	11	-0.046333625247442
1110	12	-0.09965309803272504
1110	13	-0.06212582931755861
1110	14	-0.11901549792293764
1110	15	-0.09099667144575108
1110	16	-0.03372796427975544
1110	17	-0.013094762749695121
1110	18	-0.08935947546235212
1110	19	-0.10304688682691676
1110	20	-0.07381364859700312
1110	21	-0.11025407742926063
1110	22	-0.13218811891682947
1110	23	-0.012341982359941994
1110	24	0.02857877920936147
1110	25	0.09645330828721654
1111	1	-0.03860357851064933
1111	2	-0.07721131880067489
1111	3	-0.07307647667707329
1111	4	0.053530222114353876
1111	5	-0.029106395625149162
1111	6	-0.029080374763630346
1111	7	-0.04672413261135944
1111	8	-0.03056462688041961
1111	9	-0.026482039123273182
1111	10	0.03898984057683208
1111	11	-0.1279539288381315
1111	12	-0.07887313014964548
1111	13	-0.03642739942977613
1111	14	-0.043679955725089314
1111	15	-0.10079633802620691
1111	16	-0.13230732830531622
1111	17	-0.08944497853153877
1111	18	0.002221492616286014
1111	19	-0.010568370768481827
1111	20	-0.08685739230447354
1111	21	-0.05790310710355584
1111	22	-0.08731997411153714
1111	23	-0.07872011324345607
1111	24	-0.12813596810434547
1111	25	0.00460088966922001
1112	1	-0.02075735478924301
1112	2	-0.042980764469241706
1112	3	-0.049766952913923745
1112	4	-0.08033623884296759
1112	5	0.019791871368681768
1112	6	-0.05355018468331707
1112	7	-0.060026678222669716
1112	8	-0.043025684741579084
1112	9	-0.048932094938351156
1112	10	-0.08179625174489757
1112	11	-0.017401281458418794
1112	12	-0.09723551593614843
1112	13	-0.08294861826055211
1112	14	-0.07356987779839841
1112	15	-0.07398897360111789
1112	16	-0.06347520300680998
1112	17	-0.030928826800625586
1112	18	-0.03402302094845311
1112	19	-0.15954914385228847
1112	20	-0.09765092765549355
1112	21	-0.08880342937727193
1112	22	-0.04796828274393761
1112	23	-0.04017453676571847
1112	24	0.013065021228278795
1112	25	0.04199943575854803
1113	1	-0.2833057886517807
1113	2	-0.29372438403004253
1113	3	-0.17604178374933355
1113	4	-0.14971690263774917
1113	5	-0.19312657249463427
1113	6	-0.22483633012674886
1113	7	-0.3066296564004105
1113	8	-0.2891558920663968
1113	9	-0.23427730364283406
1113	10	-0.3117066766053682
1113	11	-0.2727820660353615
1113	12	-0.44249964226629857
1113	13	-0.3529199115306554
1113	14	-0.3736928310609926
1113	15	-0.2866964915225978
1113	16	-0.30000524670745676
1113	17	-0.2993276045102249
1113	18	-0.2657645065212364
1113	19	-0.335534107296084
1113	20	-0.2511709536867528
1113	21	-0.354459750514863
1113	22	-0.34186030686133506
1113	23	-0.33865081610139214
1113	24	-0.3926678088643385
1113	25	-0.3644865193177793
1114	1	-0.3297904834899015
1114	2	-0.3508386819357625
1114	3	-0.3572822325633602
1114	4	-0.3352293865262297
1114	5	-0.34088052151464154
1114	6	-0.3075317390793657
1114	7	-0.3979879486708384
1114	8	-0.3916374606079529
1114	9	-0.4443714474709921
1114	10	-0.43926554100807635
1114	11	-0.4972442483841135
1114	12	-0.5540235363811803
1114	13	-0.38839197163930805
1114	14	-0.652741158770894
1114	15	-0.653995498942912
1114	16	-0.5981036956848769
1114	17	-0.583356130438986
1114	18	-0.6159770232432393
1114	19	-0.503106520562234
1114	20	-0.6283135834641769
1114	21	-0.5846273897292704
1114	22	-0.7082862522818516
1114	23	-0.655914495553354
1114	24	-0.6020184415325289
1114	25	-0.6013530637957061
1115	1	-0.4460375841749311
1115	2	-0.46400652780629414
1115	3	-0.46381351444991736
1115	4	-0.3171114250519125
1115	5	-0.34507567349437096
1115	6	-0.3911951158002438
1115	7	-0.4768333279899366
1115	8	-0.4829123504016408
1115	9	-0.41686635275305406
1115	10	-0.48774297042864845
1115	11	-0.5032517216359622
1115	12	-0.5259263797851332
1115	13	-0.4883667944677157
1115	14	-0.5541425299960707
1115	15	-0.5205704243816882
1115	16	-0.5845532276871239
1115	17	-0.5395628528532868
1115	18	-0.42116253877435383
1115	19	-0.49954068261867945
1115	20	-0.5315973862301675
1115	21	-0.5985221673620842
1115	22	-0.7653798324978425
1115	23	-0.6534254117358742
1115	24	-0.5880793596556373
1115	25	-0.5859002289680646
1116	1	-0.4318740712606264
1116	2	-0.5460075064025212
1116	3	-0.5348424776994136
1116	4	-0.6032457249347658
1116	5	-0.5526358398069533
1116	6	-0.5781489375605204
1116	7	-0.5668073929779354
1116	8	-0.6230715597385412
1116	9	-0.6635233639066911
1116	10	-0.7052492254700908
1116	11	-0.7291126129400922
1116	12	-0.8176338543795723
1116	13	-0.7926945957320299
1116	14	-0.8025538566660586
1116	15	-0.847345322520674
1116	16	-0.7662680800372925
1116	17	-0.7986024611017228
1116	18	-0.7381038039437584
1116	19	-0.7875677018340852
1116	20	-0.7640355144354629
1116	21	-0.8502286987517493
1116	22	-0.8468447639399557
1116	23	-0.7580681968885727
1116	24	-0.883475371810782
1116	25	-0.8987500527311312
1201	1	0.4636936777254519
1201	2	0.4909937063077301
1201	3	0.4917933577093443
1201	4	0.5227489775170753
1201	5	0.5717726051425913
1201	6	0.5642977745734825
1201	7	0.5875324789425775
1201	8	0.568984826322108
1201	9	0.6519717016483
1201	10	0.6338889372494734
1201	11	0.5752883006524812
1201	12	0.5969013198322912
1201	13	0.5861486242224032
1201	14	0.7050546791266825
1201	15	0.783273693176092
1201	16	0.6546820160364817
1201	17	0.635419152111119
1201	18	0.6606376205025484
1201	19	0.6997574861930076
1201	20	0.6802436646322931
1201	21	0.7230348833487952
1201	22	0.7041021644312977
1201	23	0.7490083126670797
1201	24	0.7355376712666626
1201	25	0.6058582530524745
1202	1	0.39760412802081646
1202	2	0.4135795252897161
1202	3	0.42288202056733226
1202	4	0.5074673542429196
1202	5	0.5045530305032386
1202	6	0.44885951392950574
1202	7	0.46290988437562675
1202	8	0.4548942207976765
1202	9	0.47577564133091244
1202	10	0.5626188076478087
1202	11	0.4446684129210894
1202	12	0.375433914954705
1202	13	0.33051401887926346
1202	14	0.5193191283618717
1202	15	0.4449881677378187
1202	16	0.4752428432407001
1202	17	0.49264373180741927
1202	18	0.5014011564003624
1202	19	0.4805165173063344
1202	20	0.3475352248647283
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2210	16	0.04476897985176009
2210	17	-0.005705544316157329
2210	18	0.04704656012350483
2210	19	0.043962630821340554
2210	20	-0.019296239215691458
2210	21	-0.05737442990012909
2210	22	-0.051776795357945105
2210	23	-0.10745808178492666
2210	24	0.0034318201764662604
2210	25	-0.066472156070148
2211	1	-0.07799983901821506
2211	2	-0.11033810355267804
2211	3	-0.1205331358977979
2211	4	-0.1358846065255861
2211	5	-0.14929004201486862
2211	6	-0.11488589773222202
2211	7	-0.06739665332278832
2211	8	-0.0643414644551683
2211	9	-0.04682829602838012
2211	10	-0.09447014860585057
2211	11	0.07160078678031567
2211	12	-0.027663912564804605
2211	13	0.044178638013207205
2211	14	0.051843482521498174
2211	15	0.061102503070529224
2211	16	0.013090558803959595
2211	17	-0.12768092298232858
2211	18	-0.0020459822275924466
2211	19	-0.0686830050228906
2211	20	-0.10085687030800727
2211	21	-0.14040463338417197
2211	22	-0.12008858708646386
2211	23	-0.0999972755843288
2211	24	-0.2282242163891084
2211	25	-0.1581482700154666
2212	1	-0.20352176498672847
2212	2	-0.1866800152976431
2212	3	-0.24017813059684556
2212	4	-0.1373399190603095
2212	5	-0.13598495998757443
2212	6	-0.16833280640846482
2212	7	-0.2230404872706515
2212	8	-0.24060355412822787
2212	9	-0.20413062999926979
2212	10	-0.22198895711588307
2212	11	-0.05884900873881094
2212	12	-0.10913735216745835
2212	13	-0.13326308617779148
2212	14	-0.10574258007828519
2212	15	-0.14348818474403657
2212	16	-0.11896935135322906
2212	17	-0.12294059884141717
2212	18	-0.2507422889055917
2212	19	-0.27378278802134304
2212	20	-0.22460774332665778
2212	21	-0.16005783511610616
2212	22	-0.2069436993994671
2212	23	-0.23767890988364115
2212	24	-0.3040779357477774
2212	25	-0.2986089880634495
2213	1	-0.4182696702527373
2213	2	-0.4299230385853612
2213	3	-0.5150688157887693
2213	4	-0.4008571062304398
2213	5	-0.46088178645447186
2213	6	-0.3715900258960403
2213	7	-0.42982393295761057
2213	8	-0.45561577404920683
2213	9	-0.4074512058937785
2213	10	-0.38553601977577756
2213	11	-0.29410220260530195
2213	12	-0.41106719696108485
2213	13	-0.32879746200703863
2213	14	-0.5457775936430806
2213	15	-0.4304834445163337
2213	16	-0.32898595155023
2213	17	-0.4486186845968021
2213	18	-0.40642690557671557
2213	19	-0.3936115770498958
2213	20	-0.4553803411782624
2213	21	-0.42567221710686454
2213	22	-0.49056237184881724
2213	23	-0.4498218938594718
2213	24	-0.4393213931416824
2213	25	-0.36648978581404634
2214	1	-0.391817711683629
2214	2	-0.45809515785983024
2214	3	-0.5289608849690737
2214	4	-0.6347283150412721
2214	5	-0.5588891014906991
2214	6	-0.5090973142163548
2214	7	-0.5593541793954273
2214	8	-0.5393777038017333
2214	9	-0.4990426471403637
2214	10	-0.5627810237172923
2214	11	-0.4800437774680404
2214	12	-0.45969758561538754
2214	13	-0.5714046568582631
2214	14	-0.6477749557212746
2214	15	-0.5295434909068675
2214	16	-0.5694206377334226
2214	17	-0.6165302541968742
2214	18	-0.7082118578577052
2214	19	-0.6407938022584432
2214	20	-0.716563263196349
2214	21	-0.7048907803470854
2214	22	-0.6363004666163832
2214	23	-0.5823141883317646
2214	24	-0.6706615511095748
2214	25	-0.6861222041456969
2215	1	-0.514980663079939
2215	2	-0.5272495131197701
2215	3	-0.6993007678900618
2215	4	-0.5701186702548782
2215	5	-0.6086359450639662
2215	6	-0.5747494563888367
2215	7	-0.5575461829388928
2215	8	-0.6452815598841823
2215	9	-0.8017933265447041
2215	10	-0.6643913392213321
2215	11	-0.634966602821379
2215	12	-0.6216098609416463
2215	13	-0.648225342899579
2215	14	-0.742172743581456
2215	15	-0.712413969397744
2215	16	-0.7700764177857664
2215	17	-0.8008114473933929
2215	18	-0.7901274183316218
2215	19	-0.8754907772200724
2215	20	-0.8012356767655504
2215	21	-0.7668382271099716
2215	22	-0.8419510890570976
2215	23	-0.7508557582603075
2215	24	-0.8507988078077133
2215	25	-0.802064000875788
2216	1	-0.6259117883874978
2216	2	-0.7172697800707191
2216	3	-0.7015545651800146
2216	4	-0.6537240530925459
2216	5	-0.6977457447787572
2216	6	-0.6324238758801073
2216	7	-0.7234729983788881
2216	8	-0.689572881999382
2216	9	-0.6669121491284358
2216	10	-0.7739984251334562
2216	11	-0.6706982429871644
2216	12	-0.6690478135637932
2216	13	-0.7355572828183199
2216	14	-0.7548235632324989
2216	15	-0.677110607492601
2216	16	-0.8680355884590156
2216	17	-0.9841660215017569
2216	18	-0.8646363742904413
2216	19	-0.8379686570411593
2216	20	-0.7298588900054312
2216	21	-0.7935874954802244
2216	22	-0.7791797338189923
2216	23	-0.9879619008617837
2216	24	-1.05884819139942
2216	25	-1.0211278923481473
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	234071.0
3	16.0
4	1939.0
5	11227.0
6	15896.0
7	16315.0
8	13798.0
9	13070.0
10	13479.0
11	15689.0
12	27004.0
13	22618.0
14	10538.0
15	10442.0
16	11075.0
17	11355.0
18	13075.0
19	15468.0
20	19469.0
21	24706.0
22	30857.0
23	37580.0
24	44178.0
25	52148.0
26	59243.0
27	67469.0
28	78574.0
29	95491.0
30	121853.0
31	157566.0
32	206557.0
33	277395.0
34	384479.0
35	547847.0
36	828270.0
37	1562944.0
38	4849109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71877702990579	17.333867429692283	12.995753691236548	25.951601849165378
2	17.09198117930938	20.287650939669042	37.051924803819645	25.56844307720193
3	18.571969495030405	23.741622458361782	28.35339141539648	29.33301663121134
4	13.527752799927878	16.37338955263583	36.10553908597022	33.99331856146607
5	16.109155846895376	34.689724743966174	32.5278377700525	16.673281639085957
6	33.092137160378634	35.85196341101853	16.192206743387846	14.863692685214994
7	29.75428559419578	30.344464130983017	20.828315152024622	19.07293512279658
8	27.26736286566184	31.65922038282154	19.68316691224904	21.390249839267575
9	27.855224641939895	14.767973335769307	18.506414241402474	38.870387780888315
10	16.869440108842856	25.729868184462934	30.1769626060847	27.22372910060951
11	36.38400965537048	22.001764619022495	21.995337468133737	19.61888825747329
12	25.418945516802648	23.658017252473723	27.644731762032237	23.27830546869139
13	29.55792295995193	20.296423761433786	24.184817883337708	25.960835395276575
14	24.357313118258453	19.979657249249826	24.62226087737922	31.040768755112495
15	25.339733068544746	26.454095755311275	22.47207104564045	25.734100130503528
16	25.575909180602153	26.033253234331415	23.099742928866412	25.29109465620002
17	25.393025676098496	24.892893425680246	24.194172076803238	25.51990882141802
18	26.77523136494542	23.91398364118284	24.78487065603623	24.52591433783552
19	27.567534212373303	24.31770438980513	24.135856486545613	23.978904911275954
20	26.63354091572453	24.172472145140606	24.15272885869673	25.041258080438134
21	26.601879808963446	24.27003476191943	24.18434216292389	24.943743266193234
22	25.90018311117816	24.17913321411911	24.675850112933638	25.24483356176909
23	24.846876593526762	23.7401171483884	24.912210873197527	26.50079538488731
24	25.12392740760107	24.920900535434708	24.550810796195094	25.404361260769125
25	25.251410662385705	24.19407201620282	24.732499136536585	25.822018184874885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4415.0
1	4415.0
2	5032.0
3	5649.0
4	5649.0
5	5649.0
6	9771.0
7	13893.0
8	13893.0
9	13893.0
10	19334.0
11	24775.0
12	24775.0
13	24775.0
14	34647.5
15	44520.0
16	44520.0
17	44520.0
18	70329.5
19	96139.0
20	96139.0
21	96139.0
22	136570.5
23	177002.0
24	177002.0
25	177002.0
26	241818.0
27	306634.0
28	306634.0
29	306634.0
30	369446.5
31	432259.0
32	432259.0
33	432259.0
34	530442.5
35	628626.0
36	628626.0
37	628626.0
38	708569.5
39	788513.0
40	788513.0
41	788513.0
42	885513.5
43	982514.0
44	982514.0
45	982514.0
46	1061125.5
47	1139737.0
48	1139737.0
49	1139737.0
50	1179495.5
51	1219254.0
52	1219254.0
53	1219254.0
54	1152265.0
55	1085276.0
56	1085276.0
57	1085276.0
58	1013719.5
59	942163.0
60	942163.0
61	942163.0
62	839577.0
63	736991.0
64	736991.0
65	736991.0
66	627601.0
67	518211.0
68	518211.0
69	518211.0
70	413295.0
71	308379.0
72	308379.0
73	308379.0
74	253117.0
75	197855.0
76	197855.0
77	197855.0
78	163860.5
79	129866.0
80	129866.0
81	129866.0
82	100558.0
83	71250.0
84	71250.0
85	71250.0
86	52614.5
87	33979.0
88	33979.0
89	33979.0
90	23767.5
91	13556.0
92	13556.0
93	13556.0
94	7340.0
95	1124.0
96	1124.0
97	1124.0
98	677.0
99	230.0
100	230.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14598886578657977
2	0.16403424886471618
3	0.17773743008297646
4	0.198014502954212
5	0.1885020514379252
6	0.06781913416494914
7	0.05519645433972782
8	0.05985169865926944
9	0.06771815272634736
10	0.06773834901406772
11	0.7020835500226703
12	0.6625594149539373
13	0.660489295462601
14	0.6604085103117197
15	0.6617111708696825
16	0.6688303622911073
17	0.6605397861819019
18	0.6705369486034771
19	0.6666592613611692
20	0.6720819646140842
21	0.6596208550906258
22	0.6626099056732382
23	0.6480685785145832
24	0.6583585871081037
25	0.6515726344340647
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9902810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.058570234611615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68889228137604	35.55037227732017
2	11.88051179646135	10.468767268150485
3	3.066342441960295	4.052959915274345
4	1.212151821335473	2.136227046212853
5	0.6714028816598769	1.479052550866616
6	0.41513183323757313	1.0974069018792414
7	0.29954293607096305	0.9238203441015012
8	0.19993662530215112	0.7047137478676839
9	0.15980599849672747	0.6336741427812275
>10	1.1249949689006067	9.852184381105243
>50	0.14088600053444894	4.3252906713387755
>100	0.11145527750948869	9.957131617846048
>500	0.015281857438659636	4.6299994544205525
>1k	0.012647765731895922	10.978306950522338
>5k	9.693542581096723E-4	2.8867802562952827
>10k+	4.615972657665106E-5	0.3233124740177833
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16361	0.1652157316963569	No Hit
GTACAAAGGGCAGGGACTTAATCAA	15434	0.15585475233797275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.039257544070824E-5
2	0.0	0.0	0.0	0.0	5.0490719300885306E-5
3	0.0	0.0	0.0	0.0	5.0490719300885306E-5
4	0.0	0.0	0.0	1.009814386017706E-5	5.0490719300885306E-5
5	0.0	0.0	0.0	1.009814386017706E-5	5.0490719300885306E-5
6	2.019628772035412E-5	0.0	0.0	1.009814386017706E-5	7.068700702123942E-5
7	2.019628772035412E-5	0.0	0.0	1.009814386017706E-5	8.078515088141648E-5
8	2.019628772035412E-5	0.0	0.0	1.009814386017706E-5	8.078515088141648E-5
9	2.019628772035412E-5	0.0	0.0	3.0294431580531182E-5	8.078515088141648E-5
10	2.019628772035412E-5	0.0	0.0	8.078515088141648E-5	8.078515088141648E-5
11	2.019628772035412E-5	0.0	0.0	8.078515088141648E-5	8.078515088141648E-5
12	2.019628772035412E-5	0.0	0.0	8.078515088141648E-5	1.4137401404247884E-4
13	3.0294431580531182E-5	0.0	0.0	8.078515088141648E-5	1.4137401404247884E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5405	0.0	16.130575	1
ACGCTTA	795	0.0	13.992042	17
GTATCAA	19700	0.0	13.800796	1
CTCGACG	825	0.0	13.715256	13
CGCTTAC	825	0.0	13.713652	18
TCGACGC	870	0.0	12.67758	14
CGACGCT	905	0.0	12.397412	15
GACGCTT	905	0.0	12.187163	16
CGTCGTA	1460	0.0	11.920763	10
AACCGCG	820	0.0	11.829538	7
TAGATCG	520	0.0	11.156044	5
GTCGTAG	1615	0.0	10.541108	11
CGAACGA	830	0.0	10.539086	16
CCGTCGT	1695	0.0	10.380251	9
ACCTCGA	1215	0.0	10.175901	11
ATACCGT	1765	0.0	10.129809	6
AGAACCG	1025	0.0	10.113148	5
CGACCAT	1595	0.0	10.077014	10
AGGCCCG	1955	0.0	10.021351	10
TACCGTC	1795	0.0	9.960358	7
>>END_MODULE