FastQCFastQC Report
Thu 2 Feb 2017
SRR4050253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9902810
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT271500.2741646058038072No Hit
TATCAACGCAGAGTACTTTTTTTTT163610.1652157316963569No Hit
GTACAAAGGGCAGGGACTTAATCAA151770.15325952936590725No Hit
GATTAAGAGGGACGGCCGGGGGCAT105890.10692924533541491No Hit
GGTATCAACGCAGAGTACTTTTTTT102890.10389980217736178No Hit
GAATAGGACCGCGGTTCTATTTTGT99080.10005240936663432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.016.3955141
GTATCAA197300.013.3886521
CTCGACG7200.011.86476613
ACGCTTA7400.011.80027417
CGTCGTA17000.011.673058510
CGCTTAC7500.011.51638318
TCGACGC7600.011.48997214
CGACGCT8200.011.22789315
ATACCGT19200.010.7860396
TAGATCG5250.010.6782045
TACCGTC18850.010.629517
GACGCTT8450.010.55872516
CGAACGA8850.010.51049516
GCTCGTA7450.010.4510579
GTCGTAG18700.010.35799911
GAGCGTT7100.010.2985137
GCATCGC14150.010.1960514
AACCGCG8800.010.1434897
CGACCAT16400.010.13168110
ACCGTCG20700.09.9997438
Produced by FastQC (version 0.11.5)