##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9902810
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287338644283793	31.0	31.0	34.0	28.0	34.0
2	31.371769225098735	33.0	31.0	34.0	28.0	34.0
3	31.4613039127278	33.0	31.0	34.0	28.0	34.0
4	34.994959006585	37.0	35.0	37.0	32.0	37.0
5	34.92128860394171	37.0	35.0	37.0	32.0	37.0
6	34.939558771702174	37.0	35.0	37.0	32.0	37.0
7	34.86553806444837	37.0	35.0	37.0	32.0	37.0
8	34.84271434067704	37.0	35.0	37.0	32.0	37.0
9	36.487427205005446	39.0	37.0	39.0	32.0	39.0
10	36.37525954754257	39.0	37.0	39.0	32.0	39.0
11	36.40205739583007	39.0	37.0	39.0	32.0	39.0
12	36.2893589799259	39.0	37.0	39.0	32.0	39.0
13	36.31751482659972	39.0	37.0	39.0	32.0	39.0
14	37.52102009429647	40.0	38.0	41.0	32.0	41.0
15	37.49554136654142	40.0	38.0	41.0	32.0	41.0
16	37.47092885756669	40.0	38.0	41.0	32.0	41.0
17	37.443914909000576	40.0	38.0	41.0	32.0	41.0
18	37.39919224947263	40.0	38.0	41.0	32.0	41.0
19	37.41152006349713	40.0	38.0	41.0	32.0	41.0
20	37.36681931694135	40.0	38.0	41.0	32.0	41.0
21	37.324129918679645	40.0	38.0	41.0	32.0	41.0
22	37.28095560754978	40.0	38.0	41.0	32.0	41.0
23	37.20969643969742	40.0	38.0	41.0	32.0	41.0
24	37.14575994086527	40.0	37.0	41.0	32.0	41.0
25	36.84155194333729	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4512693972970574
1101	2	0.41554119481796903
1101	3	0.4212377035195125
1101	4	0.35463243389404653
1101	5	0.38265207420647585
1101	6	0.40275930893295
1101	7	0.4310967835696644
1101	8	0.4423072951632747
1101	9	0.485402498832741
1101	10	0.5313163515201254
1101	11	0.4428366483888837
1101	12	0.4859716578027147
1101	13	0.5099100511620165
1101	14	0.4889859340729643
1101	15	0.5662343610984308
1101	16	0.569274916560083
1101	17	0.554699220554177
1101	18	0.6276678191374927
1101	19	0.5704461593999497
1101	20	0.6725287191595015
1101	21	0.6309410096437347
1101	22	0.6372879915027667
1101	23	0.5844757383190569
1101	24	0.6648210927124154
1101	25	0.7157858562323511
1102	1	0.40585046619911225
1102	2	0.389952760848832
1102	3	0.3754608119180105
1102	4	0.31701209354950066
1102	5	0.31091203549205915
1102	6	0.357101619972255
1102	7	0.3488671038649329
1102	8	0.43978643079756097
1102	9	0.4353284844997134
1102	10	0.507178904720206
1102	11	0.47571041573379347
1102	12	0.5220346070319906
1102	13	0.5336670730039614
1102	14	0.53890147326495
1102	15	0.6142460327424502
1102	16	0.4967242334014159
1102	17	0.5276762750280071
1102	18	0.5885414688210489
1102	19	0.604267684950976
1102	20	0.6200693273125779
1102	21	0.5660837926582118
1102	22	0.5789749420774868
1102	23	0.6029073748792442
1102	24	0.6376925743242765
1102	25	0.7974676601300601
1103	1	0.3544318218955844
1103	2	0.3169885205756131
1103	3	0.3452521619996567
1103	4	0.2385759763284767
1103	5	0.25025752225601394
1103	6	0.29141552979510976
1103	7	0.3563264479093604
1103	8	0.3051203067254207
1103	9	0.34255113026618744
1103	10	0.36910731314965517
1103	11	0.39581367181609295
1103	12	0.41566049497247803
1103	13	0.39359359093791824
1103	14	0.46354436536247334
1103	15	0.5085151785166531
1103	16	0.49193283180763103
1103	17	0.4472570692421627
1103	18	0.5434067903340676
1103	19	0.46333523796984366
1103	20	0.4789277410477766
1103	21	0.48126609411755084
1103	22	0.4853350170791657
1103	23	0.5504702068105871
1103	24	0.5434449348680417
1103	25	0.6647969875360644
1104	1	0.38572144968585675
1104	2	0.351890876699521
1104	3	0.37544697434345764
1104	4	0.3302228259723208
1104	5	0.4087846970992075
1104	6	0.4346114100562488
1104	7	0.41972253014399996
1104	8	0.42352501637100914
1104	9	0.41786269790278396
1104	10	0.4446782285659978
1104	11	0.44972885996271117
1104	12	0.46515601162128206
1104	13	0.4721406878942034
1104	14	0.5149285043378526
1104	15	0.5337750017047753
1104	16	0.5433377386225828
1104	17	0.5428276643833883
1104	18	0.5803312584521763
1104	19	0.6294267856092688
1104	20	0.6394540081111799
1104	21	0.6432646258563111
1104	22	0.5680762167696969
1104	23	0.577792051274983
1104	24	0.6106016224626813
1104	25	0.7822094698583797
1105	1	0.30823419449084355
1105	2	0.2569880856675866
1105	3	0.2580189941250062
1105	4	0.2571808487047562
1105	5	0.2326021290066791
1105	6	0.3316513439589812
1105	7	0.27245934586586884
1105	8	0.2937158564774478
1105	9	0.39293711687223976
1105	10	0.4001073891247984
1105	11	0.37843044003518145
1105	12	0.4523745286519443
1105	13	0.43806964370961055
1105	14	0.48242457120772286
1105	15	0.4630783153148741
1105	16	0.43110345379336934
1105	17	0.4271625604413529
1105	18	0.4515131319962862
1105	19	0.47119432792212024
1105	20	0.5078590038724045
1105	21	0.5349523370976854
1105	22	0.5362996835918423
1105	23	0.5885570479306637
1105	24	0.5932774455958949
1105	25	0.6056448907978407
1106	1	0.21893548303593846
1106	2	0.18218120503861357
1106	3	0.21685239741441364
1106	4	0.16808609525588025
1106	5	0.1557135188373806
1106	6	0.21007931371505606
1106	7	0.2211762985699508
1106	8	0.2769579836201643
1106	9	0.32546222506481115
1106	10	0.31094922043220663
1106	11	0.28218062247426445
1106	12	0.3676998733860799
1106	13	0.3411970568789613
1106	14	0.38066083111704074
1106	15	0.3337504907533386
1106	16	0.270430271038947
1106	17	0.31799753367806716
1106	18	0.3876532312891712
1106	19	0.3945040877421704
1106	20	0.3789931020772528
1106	21	0.35113515884947333
1106	22	0.403060652412897
1106	23	0.40346505159264723
1106	24	0.4459644742588864
1106	25	0.5466574322405364
1107	1	0.1895109322900268
1107	2	0.15677394400356448
1107	3	0.16395774035242994
1107	4	0.1772589228683259
1107	5	0.2007402139779657
1107	6	0.22920180346985575
1107	7	0.19562698732563888
1107	8	0.25748324473417483
1107	9	0.25850042787840977
1107	10	0.30660091426276637
1107	11	0.2813888706307779
1107	12	0.30831314519838315
1107	13	0.1908224331384858
1107	14	0.26075119991232043
1107	15	0.21215053918560756
1107	16	0.18838585393928042
1107	17	0.18303425064012657
1107	18	0.2596014191037028
1107	19	0.21708402514770597
1107	20	0.23912894163904497
1107	21	0.03908109658142678
1107	22	-0.047537265545706475
1107	23	0.17123484996872662
1107	24	0.23919832653235318
1107	25	0.39890442201240006
1108	1	0.15028314219897254
1108	2	0.10961535678397283
1108	3	0.110472772556232
1108	4	0.14274993950098036
1108	5	0.17288542720021383
1108	6	0.12808173647985654
1108	7	0.07841790243926283
1108	8	0.1251013247843531
1108	9	0.19036426101367
1108	10	0.13937238013525644
1108	11	0.17720642655118723
1108	12	0.10633901271135215
1108	13	0.05204858187835271
1108	14	0.016582100216467666
1108	15	0.0901873606180601
1108	16	0.05327255693287469
1108	17	0.07219320132784901
1108	18	0.08489860951998907
1108	19	0.052094685380311034
1108	20	0.08423753653814003
1108	21	0.15909961377306558
1108	22	0.12827364089402948
1108	23	0.017559549026508137
1108	24	0.08375610378178067
1108	25	0.36025957032116196
1109	1	-0.06732671796685352
1109	2	-0.05043209583804398
1109	3	-0.07724839992570054
1109	4	-0.09034688291313131
1109	5	-0.0401256384729507
1109	6	-0.0395312575720439
1109	7	-0.0280268033272435
1109	8	-0.07016818767201016
1109	9	-0.03580650295442922
1109	10	-0.09893537900001803
1109	11	-0.08406988041461005
1109	12	-0.100066990871575
1109	13	-0.09178150789744421
1109	14	-0.11055952245212097
1109	15	-0.10439600537810634
1109	16	-0.0810806919118221
1109	17	-0.08631040211736263
1109	18	-0.17443575744287898
1109	19	-0.14954585596665027
1109	20	-0.1578838782394527
1109	21	-0.12810795057242785
1109	22	-0.08972890654506216
1109	23	-0.0779619313619122
1109	24	-0.04821354536090183
1109	25	0.07912371382835204
1110	1	-0.022953628809929683
1110	2	-0.09543363282702444
1110	3	-0.02281822079805096
1110	4	-0.014749812991702527
1110	5	0.03027887567601084
1110	6	0.06805211159129243
1110	7	0.08293027717929391
1110	8	0.011677356637306957
1110	9	-0.018369282555887878
1110	10	0.0067704393928949
1110	11	0.05716158034012864
1110	12	0.1272484614722842
1110	13	0.1117180658036716
1110	14	0.09918270033914922
1110	15	0.08065980319819488
1110	16	0.07343171202037979
1110	17	0.09473032213702481
1110	18	0.11731382633489318
1110	19	0.07432149914698982
1110	20	0.13618684289374272
1110	21	0.09363696030719382
1110	22	0.10317938626324263
1110	23	0.15219452980513637
1110	24	0.11200001159360795
1110	25	0.28167905039300933
1111	1	-0.050403821835612206
1111	2	-0.0693675254827042
1111	3	-0.06811299594868103
1111	4	-0.05594993113319191
1111	5	-0.07484281485547228
1111	6	-0.01356014957377738
1111	7	-0.01776960640390257
1111	8	0.015890500404218244
1111	9	-0.04798798410203631
1111	10	-0.0018455381695829942
1111	11	-0.02317773665193812
1111	12	-0.024920497722803248
1111	13	0.0058065244182756715
1111	14	-0.06285683821717214
1111	15	-0.048288058793914956
1111	16	-0.008946521713227185
1111	17	-0.024154224469114638
1111	18	0.04325606704497176
1111	19	0.02720042110141918
1111	20	0.05263287127090166
1111	21	-0.0018204154262519978
1111	22	-0.025271845571602114
1111	23	-0.043369208459708375
1111	24	-0.06261948249250793
1111	25	0.09509366362811278
1112	1	-0.0972192350150074
1112	2	-0.04780550955063845
1112	3	-0.07154538675853672
1112	4	-0.03708064702724556
1112	5	-0.06745568997459372
1112	6	-0.0021702253837290186
1112	7	-0.06683583613297372
1112	8	-0.02700947919944241
1112	9	0.05096403112804637
1112	10	-0.006984760033297732
1112	11	-0.04722731377201228
1112	12	-0.00629222392434059
1112	13	-0.06839370406384404
1112	14	-0.007042376217611945
1112	15	-0.002171410513355454
1112	16	0.01330087457463236
1112	17	0.024525060776653618
1112	18	0.017499892275580464
1112	19	0.040631500177767066
1112	20	-0.041925931292340124
1112	21	-0.017509643174179246
1112	22	-0.002178969511135165
1112	23	-0.04488038212252121
1112	24	-0.01379647638981396
1112	25	0.10443274261927371
1113	1	-0.19877808126398122
1113	2	-0.1790090951740808
1113	3	-0.22652955359655635
1113	4	-0.1635566142574234
1113	5	-0.14059352550416548
1113	6	-0.161489652779089
1113	7	-0.15248750312080261
1113	8	-0.17101135747571306
1113	9	-0.21027144341594095
1113	10	-0.19065190007042077
1113	11	-0.20400294813015307
1113	12	-0.1725132467376298
1113	13	-0.19155530217096128
1113	14	-0.19108039822278045
1113	15	-0.15574339795561087
1113	16	-0.18689999141231084
1113	17	-0.1982250040526452
1113	18	-0.20385534236728375
1113	19	-0.14665957891523362
1113	20	-0.21002183264255336
1113	21	-0.25126029808037487
1113	22	-0.18606809477826403
1113	23	-0.13826975728175483
1113	24	-0.15080497342667343
1113	25	-0.10827038999268268
1114	1	-0.15072146319004887
1114	2	-0.1253915396625267
1114	3	-0.22351218023974084
1114	4	-0.12957522315281977
1114	5	-0.17746099024478923
1114	6	-0.1561824061819692
1114	7	-0.2034827414486955
1114	8	-0.20295603540658647
1114	9	-0.17719714493820504
1114	10	-0.23310049838718783
1114	11	-0.19102863409386828
1114	12	-0.17936992932159512
1114	13	-0.19458344756536405
1114	14	-0.22061848611049584
1114	15	-0.28726995395897603
1114	16	-0.26858090811767
1114	17	-0.24129666985356124
1114	18	-0.21780485992529464
1114	19	-0.3306444878289838
1114	20	-0.25618539016733877
1114	21	-0.12462035225291856
1114	22	-0.14817580870082026
1114	23	-0.2372496002639366
1114	24	-0.24864887239717604
1114	25	-0.1526023498243987
1115	1	-0.291780752202051
1115	2	-0.2710417978898896
1115	3	-0.2588035342555237
1115	4	-0.23410486875373238
1115	5	-0.2890981300650779
1115	6	-0.26787383049674673
1115	7	-0.28600339570478894
1115	8	-0.2744118334645762
1115	9	-0.38308868448940103
1115	10	-0.32587511979821926
1115	11	-0.36540307588776955
1115	12	-0.2939233408124977
1115	13	-0.3124609415510662
1115	14	-0.45032407009193776
1115	15	-0.42360212311464096
1115	16	-0.47894599857180253
1115	17	-0.47619480056238217
1115	18	-0.49127744364795944
1115	19	-0.4041425310948199
1115	20	-0.42110261005898764
1115	21	-0.42929751250999715
1115	22	-0.3848563199305204
1115	23	-0.3600143303139731
1115	24	-0.43324851474188364
1115	25	-0.29043993404567914
1116	1	-0.27646691796855904
1116	2	-0.23676666919603662
1116	3	-0.24794332242182548
1116	4	-0.2120190312202226
1116	5	-0.2577174149559127
1116	6	-0.2576214696205881
1116	7	-0.23657783463973914
1116	8	-0.31705467170444734
1116	9	-0.3480196586877824
1116	10	-0.34753519650918463
1116	11	-0.34461560539056535
1116	12	-0.3392511506978053
1116	13	-0.40984387157502056
1116	14	-0.49246367199940266
1116	15	-0.4850489510370011
1116	16	-0.4750777388135461
1116	17	-0.44433754867883835
1116	18	-0.40774883346218616
1116	19	-0.42416280404228957
1116	20	-0.4479101999138493
1116	21	-0.4807126514741782
1116	22	-0.5757529985678929
1116	23	-0.5287833851206969
1116	24	-0.5667554788600597
1116	25	-0.3852216209904
1201	1	0.3080437317203675
1201	2	0.3182067585888291
1201	3	0.3283588529853425
1201	4	0.22083341442463933
1201	5	0.23883065621837574
1201	6	0.26290985727133176
1201	7	0.32003247552376735
1201	8	0.3130879854938584
1201	9	0.35622874714484
1201	10	0.3574959793016461
1201	11	0.3686350348993557
1201	12	0.4030031834929986
1201	13	0.3306800185029317
1201	14	0.4216292235650201
1201	15	0.44362449767361056
1201	16	0.404756049469583
1201	17	0.46455165910780494
1201	18	0.4622817244060329
1201	19	0.4197347284775148
1201	20	0.4777290071987039
1201	21	0.4723236328670737
1201	22	0.4291586383684134
1201	23	0.452062441626623
1201	24	0.48658203928101784
1201	25	0.4853460145099433
1202	1	0.34793555353272154
1202	2	0.3497356923153987
1202	3	0.35854151152030767
1202	4	0.27641056617640203
1202	5	0.22650905612079697
1202	6	0.3487661556181294
1202	7	0.34276343571533374
1202	8	0.3744931948812038
1202	9	0.3855668217550985
1202	10	0.397465805913896
1202	11	0.42895422156127694
1202	12	0.4877524103543607
1202	13	0.47348469910966884
1202	14	0.47775049450897455
1202	15	0.4211922044211178
1202	16	0.4893012219160724
1202	17	0.5362757655665646
1202	18	0.4739240889867986
1202	19	0.40453414195760473
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2210	6	-0.03230229097509607
2210	7	-0.0721980453059885
2210	8	-0.15347284533273609
2210	9	-0.13651013647004362
2210	10	-0.06867978596848445
2210	11	-0.07601800578415663
2210	12	-0.12255838315193301
2210	13	-0.11669829506242735
2210	14	-0.05463320051796927
2210	15	-0.0828219572451232
2210	16	-0.03951772070346493
2210	17	-0.01898629272468355
2210	18	-0.04595829724914324
2210	19	-0.041068876651102926
2210	20	0.0047025063008589996
2210	21	-0.06158356252128527
2210	22	-0.07095817755881484
2210	23	-0.07590475536299834
2210	24	-0.08655410998716917
2210	25	-0.179928011102632
2211	1	-0.13678573058003352
2211	2	-0.1448083560433382
2211	3	-0.10308859291846062
2211	4	-0.09733287928936818
2211	5	-0.03919136798116796
2211	6	-0.10315391913883332
2211	7	-0.11819752560920449
2211	8	-0.18614284944800374
2211	9	-0.18917602132966493
2211	10	-0.15738265551701147
2211	11	-0.20210929120347032
2211	12	-0.1657454536387064
2211	13	-0.12939083134640583
2211	14	-0.1565593516842796
2211	15	-0.18898867453765433
2211	16	-0.09506975484539026
2211	17	-0.14579674749130334
2211	18	-0.14353059078442243
2211	19	-0.11427948916286113
2211	20	-0.1109913148555961
2211	21	-0.12138626135220676
2211	22	-0.1970604436683132
2211	23	-0.1772302017055054
2211	24	-0.20598981187919208
2211	25	-0.254042967872401
2212	1	-0.19827599452229094
2212	2	-0.13992187574402237
2212	3	-0.15740677315545426
2212	4	-0.13810843580378673
2212	5	-0.15172797958911843
2212	6	-0.17384842571708958
2212	7	-0.17518475418664536
2212	8	-0.1693064903530086
2212	9	-0.17991999251848512
2212	10	-0.20362681890105705
2212	11	-0.21122796155270152
2212	12	-0.22711743311636212
2212	13	-0.17154222540658282
2212	14	-0.18976662075898787
2212	15	-0.18408044707979343
2212	16	-0.18553935852787617
2212	17	-0.2326172410049594
2212	18	-0.15339174235612063
2212	19	-0.1491612077332931
2212	20	-0.21706240556590473
2212	21	-0.23449553257095346
2212	22	-0.21677843162136412
2212	23	-0.22904994509977428
2212	24	-0.29435573497747214
2212	25	-0.2951197200034983
2213	1	-0.3010841086980385
2213	2	-0.2963240202049491
2213	3	-0.32038145716736466
2213	4	-0.27437280201682057
2213	5	-0.23930944866529558
2213	6	-0.28056629172598946
2213	7	-0.2979949429607771
2213	8	-0.2727531584550249
2213	9	-0.36417309743139725
2213	10	-0.44362402549047886
2213	11	-0.38026326443826974
2213	12	-0.3364143163516857
2213	13	-0.32511704190090285
2213	14	-0.36086334835169254
2213	15	-0.4153812764167526
2213	16	-0.4498242221184867
2213	17	-0.39823378716145896
2213	18	-0.3220635813334596
2213	19	-0.39007667303222604
2213	20	-0.34318027844467736
2213	21	-0.3394805315151288
2213	22	-0.40086748504140957
2213	23	-0.4100600521857416
2213	24	-0.3726958525839663
2213	25	-0.3734423446946238
2214	1	-0.34144609100405177
2214	2	-0.3573787863602078
2214	3	-0.34754279272873134
2214	4	-0.29717893159850917
2214	5	-0.32674950804049274
2214	6	-0.3862558147701165
2214	7	-0.4055484827458926
2214	8	-0.4741679892261885
2214	9	-0.505348707341021
2214	10	-0.4902718958663357
2214	11	-0.4581841663088966
2214	12	-0.49430995155725554
2214	13	-0.48795463770306213
2214	14	-0.5527113209067664
2214	15	-0.5622245828365493
2214	16	-0.5053867457693926
2214	17	-0.5369901578085106
2214	18	-0.538224702435592
2214	19	-0.5019889760389731
2214	20	-0.5194492220389648
2214	21	-0.6108384390956445
2214	22	-0.5985082284903385
2214	23	-0.642863905425358
2214	24	-0.6372944734573665
2214	25	-1.4630529218534676
2215	1	-0.4432932038822166
2215	2	-0.4239432940601233
2215	3	-0.4334913282232442
2215	4	-0.34785379077763423
2215	5	-0.4290700752759875
2215	6	-0.4312262642351712
2215	7	-0.4139104997002079
2215	8	-0.46122788385578417
2215	9	-0.5524018386377776
2215	10	-0.4912697313892451
2215	11	-0.5434029181844267
2215	12	-0.49004095131262204
2215	13	-0.5054702588258593
2215	14	-0.5923269094939698
2215	15	-0.530906003185585
2215	16	-0.5567817156327237
2215	17	-0.652680282745898
2215	18	-0.6337777637123665
2215	19	-0.5749947457550348
2215	20	-0.6462409735105297
2215	21	-0.72753541265336
2215	22	-0.7002089171429233
2215	23	-0.723321520176512
2215	24	-0.7128801826438789
2215	25	-0.7025078646143328
2216	1	-0.4566433707543318
2216	2	-0.3255988553471738
2216	3	-0.32530738520965485
2216	4	-0.27936981422259066
2216	5	-0.315459785193255
2216	6	-0.3602231538402165
2216	7	-0.35320938462020024
2216	8	-0.40452811128437105
2216	9	-0.44378796826699585
2216	10	-0.40245211661342495
2216	11	-0.5100885792892029
2216	12	-0.46790552596763035
2216	13	-0.5194523862396849
2216	14	-0.5371916769938423
2216	15	-0.5653942005501165
2216	16	-0.5945565624299363
2216	17	-0.6060380003545802
2216	18	-0.5565493446812795
2216	19	-0.522881645543201
2216	20	-0.49539406608975156
2216	21	-0.4900411940910345
2216	22	-0.5883272848091465
2216	23	-0.5959703644773029
2216	24	-0.598229244065891
2216	25	-0.7650673160879293
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101259.0
3	22.0
4	1884.0
5	15162.0
6	18410.0
7	14872.0
8	12088.0
9	10427.0
10	9549.0
11	9032.0
12	8312.0
13	7976.0
14	7803.0
15	8318.0
16	8538.0
17	9446.0
18	10379.0
19	12354.0
20	15798.0
21	20726.0
22	26522.0
23	32406.0
24	38770.0
25	46134.0
26	54412.0
27	62935.0
28	75779.0
29	94829.0
30	124510.0
31	166858.0
32	222810.0
33	304077.0
34	424213.0
35	605102.0
36	913677.0
37	1638367.0
38	4769054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.37422209543912	17.9959093594212	13.54785289856068	26.082015646579
2	17.234471993709093	20.915445658756543	36.18196861149707	25.668113736037302
3	18.60573339877479	23.936293549370934	28.538373133454115	28.919599918400156
4	13.815686127346016	16.95088049829071	35.68527363738645	33.54815973697682
5	15.97619372034833	35.053437772995345	32.491638622016225	16.478729884640103
6	32.97695614619001	36.17963075901781	16.045291663019647	14.798121431772529
7	29.595624024942875	30.523717259020135	20.791367756918746	19.089290959118248
8	27.354106998638063	31.825658858260482	19.538590139680252	21.281644003421203
9	27.771274740263785	14.821301950651963	18.48713272416142	38.92029058492283
10	16.882696583453296	25.97035687864817	30.12825433739939	27.018692200499146
11	36.40078463121737	21.993838965929037	22.019721249223252	19.585655153630345
12	25.452958601549064	23.78072685305608	27.60166775678631	23.164646788608543
13	29.66593756434852	20.25137821343709	24.24271098817012	25.839973234044265
14	24.373955498492244	19.925571961931446	24.772856740215126	30.92761579936119
15	25.338415293626504	26.513151556610374	22.532077324483833	25.616355825279292
16	25.625964033387444	26.003609627801566	23.156503581048888	25.213922757762102
17	25.394808447529922	24.887183520831478	24.29878172239234	25.419226309246262
18	26.731815897729216	23.87848477797921	25.017177487372884	24.372521836918693
19	27.52714633523212	24.231253553284372	24.394348674770093	23.847251436713417
20	26.614486468058363	24.023391012083987	24.40375549590123	24.95836702395642
21	26.567218045963152	24.137865222388573	24.450897836794073	24.8440188948542
22	25.894715922998813	24.013784777004663	24.917457755416443	25.174041544580085
23	24.818478162193067	23.574194094338743	25.23005860058006	26.37726914288813
24	25.17238938324767	24.738700763941086	24.822415078190076	25.266494774621172
25	25.230324309815906	24.049429946195463	25.052472619881	25.66777312410763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1686.0
1	1686.0
2	2237.5
3	2789.0
4	2789.0
5	2789.0
6	6269.0
7	9749.0
8	9749.0
9	9749.0
10	13161.0
11	16573.0
12	16573.0
13	16573.0
14	25027.0
15	33481.0
16	33481.0
17	33481.0
18	60780.0
19	88079.0
20	88079.0
21	88079.0
22	132815.5
23	177552.0
24	177552.0
25	177552.0
26	246119.0
27	314686.0
28	314686.0
29	314686.0
30	379793.5
31	444901.0
32	444901.0
33	444901.0
34	540072.0
35	635243.0
36	635243.0
37	635243.0
38	715968.5
39	796694.0
40	796694.0
41	796694.0
42	891204.5
43	985715.0
44	985715.0
45	985715.0
46	1065062.0
47	1144409.0
48	1144409.0
49	1144409.0
50	1182541.0
51	1220673.0
52	1220673.0
53	1220673.0
54	1153547.5
55	1086422.0
56	1086422.0
57	1086422.0
58	1014186.0
59	941950.0
60	941950.0
61	941950.0
62	837931.5
63	733913.0
64	733913.0
65	733913.0
66	625301.5
67	516690.0
68	516690.0
69	516690.0
70	412018.5
71	307347.0
72	307347.0
73	307347.0
74	251167.0
75	194987.0
76	194987.0
77	194987.0
78	161759.5
79	128532.0
80	128532.0
81	128532.0
82	100081.0
83	71630.0
84	71630.0
85	71630.0
86	53037.0
87	34444.0
88	34444.0
89	34444.0
90	23950.5
91	13457.0
92	13457.0
93	13457.0
94	7255.0
95	1053.0
96	1053.0
97	1053.0
98	604.0
99	155.0
100	155.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03168797543323562
2	0.002696204410667275
3	0.00838145940394696
4	0.16695261244030735
5	0.02250876266433467
6	0.08979269520469443
7	0.053651438329120726
8	0.02219572020466918
9	0.04056424388633126
10	0.026638903503147085
11	0.04187700258815427
12	0.033172402580681645
13	0.006856639681060225
14	0.003625233645803565
15	0.003352583761578784
16	0.004079650119511532
17	0.0022518860808194846
18	0.003170817172095597
19	0.0
20	0.002049923203615943
21	8.078515088141648E-5
22	2.019628772035412E-5
23	7.068700702123942E-5
24	8.38145940394696E-4
25	0.1494121365551798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9902810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.26435198469034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1032787209962	34.65616445712936
2	12.400870210874041	10.730312274394313
3	3.080256381659876	3.9979588889766458
4	1.253833343875642	2.169851484783082
5	0.6497043574506657	1.4054519003366335
6	0.4246440971902869	1.1023171013436976
7	0.2820336035394405	0.8541400766528675
8	0.20815264804986516	0.7204471545419786
9	0.16062651074558448	0.6254461709072651
>10	1.1460590906635477	9.901810682955702
>50	0.1473896324394633	4.458616175420855
>100	0.11335018859878125	9.953740711482324
>500	0.015536426971541506	4.643926684325305
>1k	0.013207265716642522	11.27163095593129
>5k	9.400188698923087E-4	2.6992117554710466
>10k+	1.1750235873653859E-4	0.8089735253478025
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	27150	0.2741646058038072	No Hit
TATCAACGCAGAGTACTTTTTTTTT	16361	0.1652157316963569	No Hit
GTACAAAGGGCAGGGACTTAATCAA	15177	0.15325952936590725	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	10589	0.10692924533541491	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10289	0.10389980217736178	No Hit
GAATAGGACCGCGGTTCTATTTTGT	9908	0.10005240936663432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.039257544070824E-5
2	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
3	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
4	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
5	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
6	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
7	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
8	0.0	1.009814386017706E-5	0.0	0.0	4.039257544070824E-5
9	0.0	1.009814386017706E-5	0.0	1.009814386017706E-5	6.0588863161062364E-5
10	0.0	1.009814386017706E-5	0.0	5.0490719300885306E-5	6.0588863161062364E-5
11	1.009814386017706E-5	1.009814386017706E-5	0.0	6.0588863161062364E-5	6.0588863161062364E-5
12	1.009814386017706E-5	1.009814386017706E-5	0.0	6.0588863161062364E-5	2.1206102106371828E-4
13	1.009814386017706E-5	1.009814386017706E-5	0.0	8.078515088141648E-5	2.524535965044265E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6015	0.0	16.395514	1
GTATCAA	19730	0.0	13.388652	1
CTCGACG	720	0.0	11.864766	13
ACGCTTA	740	0.0	11.800274	17
CGTCGTA	1700	0.0	11.6730585	10
CGCTTAC	750	0.0	11.516383	18
TCGACGC	760	0.0	11.489972	14
CGACGCT	820	0.0	11.227893	15
ATACCGT	1920	0.0	10.786039	6
TAGATCG	525	0.0	10.678204	5
TACCGTC	1885	0.0	10.62951	7
GACGCTT	845	0.0	10.558725	16
CGAACGA	885	0.0	10.510495	16
GCTCGTA	745	0.0	10.451057	9
GTCGTAG	1870	0.0	10.357999	11
GAGCGTT	710	0.0	10.298513	7
GCATCGC	1415	0.0	10.19605	14
AACCGCG	880	0.0	10.143489	7
CGACCAT	1640	0.0	10.131681	10
ACCGTCG	2070	0.0	9.999743	8
>>END_MODULE