FastQCFastQC Report
Thu 2 Feb 2017
SRR4050252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038392
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA530305.106934568062928No Hit
CTTCCGTACTCTGCGTTGATACCAC343923.312044006502361No Hit
GAGTACGGAAGCAGTGGTATCAACG247782.386189415943112No Hit
TCCGTACTCTGCGTTGATACCACTG241462.3253260810946155No Hit
GTACATGGGAAGCAGTGGTATCAAC207812.001267344124377No Hit
CCCATGTACTCTGCGTTGATACCAC198731.9138244516521699No Hit
GCTTCCGTACTCTGCGTTGATACCA172661.6627631954021216No Hit
GCGTTGATACCACTGCTTCCGTACT128851.2408608694982242No Hit
GTATCAACGCAGAGTACGGAAGCAG110511.0642416351435682No Hit
GTACATGGAAGCAGTGGTATCAACG92640.892148629804544No Hit
ACTCTGCGTTGATACCACTGCTTCC91020.8765475851123661No Hit
TATCAACGCAGAGTACGGAAGCAGT86120.8293592400557785No Hit
TCCATGTACTCTGCGTTGATACCAC82920.7985423616514765No Hit
CATGTACTCTGCGTTGATACCACTG65900.634635089638595No Hit
GAGTACATGGGAAGCAGTGGTATCA64430.6204785861216188No Hit
GGTATCAACGCAGAGTACGGAAGCA60450.5821500936062681No Hit
ATACCACTGCTTCCGTACTCTGCGT58910.5673194708741978No Hit
CAGTGGTATCAACGCAGAGTACGGA58570.5640451775437407No Hit
GTATCAACGCAGAGTACATGGGAAG55770.5370804089399764No Hit
ACGCAGAGTACGGAAGCAGTGGTAT53720.5173383462122204No Hit
GCGTTGATACCACTGCTTCCCATGT53710.5172420434672069No Hit
GTACTCTGCGTTGATACCACTGCTT51010.4912403023135771No Hit
TATCAACGCAGAGTACATGGGAAGC46210.4450149847071241No Hit
AAGCAGTGGTATCAACGCAGAGTAC45410.4373107651060486No Hit
GAAGCAGTGGTATCAACGCAGAGTA41150.3962857957303215No Hit
GTGGTATCAACGCAGAGTACGGAAG40600.39098914475458213No Hit
ACGCAGAGTACATGGGAAGCAGTGG34860.33571136911686533No Hit
GATACCACTGCTTCCGTACTCTGCG33740.32492546167535963No Hit
GCTTCCCATGTACTCTGCGTTGATA33580.3233846177551445No Hit
GTTGATACCACTGCTTCCGTACTCT33470.3223252875599966No Hit
CCACTGCTTCCGTACTCTGCGTTGA31280.30123498640205243No Hit
GTACGGGAAGCAGTGGTATCAACGC31030.2988274177767163No Hit
GAGTACATGGAAGCAGTGGTATCAA29170.28091510720421575No Hit
ATCAACGCAGAGTACGGAAGCAGTG29160.28081880445920226No Hit
AAAAAGTACTCTGCGTTGATACCAC28880.2781223275988259No Hit
GGTATCAACGCAGAGTACATGGGAA28570.27513694250340914No Hit
CAGTGGTATCAACGCAGAGTACATG28130.2708996217228176No Hit
TTCCCGTACTCTGCGTTGATACCAC26160.25192798095516916No Hit
CGGAAGCAGTGGTATCAACGCAGAG25080.24152728449371724No Hit
CTGCTTCCGTACTCTGCGTTGATAC24670.23757887194816601No Hit
CCCGTACTCTGCGTTGATACCACTG24450.23546021155787022No Hit
GCAGAGTACGGAAGCAGTGGTATCA22210.21388839667485882No Hit
ATACCACTGCTTCCCATGTACTCTG20360.19607238884737171No Hit
CATGGAAGCAGTGGTATCAACGCAG19830.1909683433616592No Hit
GTATCAACGCAGAGTACGGGAAGCA19580.18856077473632307No Hit
CTGCTTCCCATGTACTCTGCGTTGA19540.18817556375626932No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18980.18278261003551646No Hit
GCTTCCATGTACTCTGCGTTGATAC18600.1791231057250056No Hit
GGGAAGCAGTGGTATCAACGCAGAG18520.17835268376489802No Hit
CTTCCCGTACTCTGCGTTGATACCA18500.17816007827487115No Hit
CACTGCTTCCGTACTCTGCGTTGAT18450.17767856454980394No Hit
GCGTTGATACCACTGCTTCCATGTA17850.1719003998489973No Hit
TACCACTGCTTCCCATGTACTCTGC17390.16747047357837888No Hit
GAGTACGGGAAGCAGTGGTATCAAC17290.16650744612824445No Hit
GTGGTATCAACGCAGAGTACATGGG16860.16236642809266635No Hit
TGATACCACTGCTTCCGTACTCTGC16800.16178861162258568No Hit
GTATCAACGCAGAGTACTTTTTTTT16800.16178861162258568No Hit
AAAGTACTCTGCGTTGATACCACTG16780.1615960061325588No Hit
CCATGTACTCTGCGTTGATACCACT16130.15533632770668496No Hit
GTATCAACGCAGAGTACATGGAAGC16100.1550474194716446No Hit
GCAGTGGTATCAACGCAGAGTACGG15940.15350657555142952No Hit
TACCACTGCTTCCGTACTCTGCGTT14890.1433947873250179No Hit
GCGTTGATACCACTGCTTCCCGTAC14590.1405057049746146No Hit
CATGGGAAGCAGTGGTATCAACGCA14460.13925376928943983No Hit
GCAGTGGTATCAACGCAGAGTACAT14460.13925376928943983No Hit
GCAGAGTACATGGGAAGCAGTGGTA14380.13848334732933226No Hit
CTGCGTTGATACCACTGCTTCCGTA13340.1284678618479341No Hit
TATCAACGCAGAGTACATGGAAGCA13190.12702332067273245No Hit
GTTGATACCACTGCTTCCCATGTAC13110.1262528987126249No Hit
CTTCCCATGTACTCTGCGTTGATAC12980.12500096302745012No Hit
TATCAACGCAGAGTACGGGAAGCAG12800.12326751361720815No Hit
CCACTGCTTCCCATGTACTCTGCGT12230.11777825715144186No Hit
GATACCACTGCTTCCCATGTACTCT12170.11720044068136118No Hit
GCTTCCCGTACTCTGCGTTGATACC11700.11267421166572933No Hit
TATCAACGCAGAGTACTTTTTTTTT11480.11055555127543355No Hit
TCAACGCAGAGTACGGAAGCAGTGG10920.1051625975546807No Hit
ATCAACGCAGAGTACATGGGAAGCA10910.10506629480966725No Hit
ACGCAGAGTACATGGAAGCAGTGGT10740.10342914814443871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT250.006010537519.0110413
CGAACGA702.4836481E-816.2943916
TCTAGGA509.0272166E-515.13627052
AACGAAC704.4027183E-714.93724614
TTAGCCT456.97841E-414.7158174
CGGACCA602.5305293E-514.2714129
TCCGATA801.2635792E-714.2714118
CCGATAA801.2635792E-714.2714119
ACGAACG801.2778764E-714.2582815
CCTTACT400.005426692314.1902522
CTGTAGA955.0695235E-913.9648551
GATAACG759.5014184E-713.954267511
CGACCAA551.9495201E-413.82621214
TTCCGAT852.6472117E-713.4319167
GGGTAGG1153.9108272E-1013.1842131
CGAGCGA655.3728047E-513.1736110
TTAACCA655.6681303E-513.0986954
CGATAAC801.9641557E-613.08212710
CGCCAGT801.9845647E-613.07008918
AACCGCG957.233757E-813.0195327
Produced by FastQC (version 0.11.5)