##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050252_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038392
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.544328153529687	31.0	31.0	34.0	27.0	34.0
2	30.63624430850777	31.0	31.0	34.0	27.0	34.0
3	30.762418238969484	31.0	31.0	34.0	27.0	34.0
4	34.290520342991854	37.0	35.0	37.0	30.0	37.0
5	34.29931278361158	37.0	35.0	37.0	30.0	37.0
6	34.303098444518064	37.0	35.0	37.0	30.0	37.0
7	34.18367244739944	37.0	35.0	37.0	30.0	37.0
8	34.119029229809165	37.0	35.0	37.0	30.0	37.0
9	35.5959733896255	39.0	37.0	39.0	30.0	39.0
10	35.54423281381212	39.0	37.0	39.0	30.0	39.0
11	35.39564056733873	39.0	37.0	39.0	30.0	39.0
12	35.350820306782026	39.0	37.0	39.0	30.0	39.0
13	35.309754890253394	39.0	37.0	39.0	30.0	39.0
14	36.48365068297907	40.0	37.0	41.0	30.0	41.0
15	36.4258671099161	40.0	37.0	41.0	29.0	41.0
16	36.314492022280604	40.0	37.0	41.0	29.0	41.0
17	36.379595566992045	40.0	37.0	41.0	30.0	41.0
18	36.320733403184924	40.0	37.0	41.0	29.0	41.0
19	36.36106306674166	40.0	37.0	41.0	29.0	41.0
20	36.359365249347064	40.0	37.0	41.0	29.0	41.0
21	36.30306281250241	40.0	37.0	41.0	29.0	41.0
22	36.26657562847171	40.0	37.0	41.0	30.0	41.0
23	36.17191291920585	40.0	37.0	41.0	29.0	41.0
24	36.092085647809306	40.0	37.0	41.0	29.0	41.0
25	35.87197513077913	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7905126509350993
1101	2	0.7109603306078718
1101	3	0.6965657824580447
1101	4	0.736435056396445
1101	5	0.7919043825511451
1101	6	0.7841912448757355
1101	7	0.8487364891685161
1101	8	0.8377806349711818
1101	9	0.8821929649629112
1101	10	0.8656219833891967
1101	11	0.6663247838140833
1101	12	0.9850511851412378
1101	13	0.8562794507520763
1101	14	1.0230294666842497
1101	15	1.0366712601647023
1101	16	1.0528642337290393
1101	17	1.0352397140772922
1101	18	0.8987882231539004
1101	19	1.0129046106563564
1101	20	0.9125511026241284
1101	21	0.8526177784421733
1101	22	0.8759014838024157
1101	23	1.016027799993502
1101	24	0.9490390387849885
1101	25	1.11889441588972
1102	1	0.45437811263560945
1102	2	0.4550720398945458
1102	3	0.4696316069650379
1102	4	0.4721422364537915
1102	5	0.5485424245664774
1102	6	0.46796626096795535
1102	7	0.39893691049217495
1102	8	0.34919514137880725
1102	9	0.41500647072685126
1102	10	0.33387915905775856
1102	11	0.38327903801268803
1102	12	0.5925179766445439
1102	13	0.6162685153550385
1102	14	0.79189028358401
1102	15	0.8696541000031957
1102	16	0.87935623882003
1102	17	0.7462096728226797
1102	18	0.6859054842575318
1102	19	0.7533306522620649
1102	20	0.7490358296415138
1102	21	0.8109060047866095
1102	22	0.8049585526771352
1102	23	0.7302884354900243
1102	24	0.9540687728244279
1102	25	0.8637348468687165
1103	1	0.5564868876803573
1103	2	0.6215727039051693
1103	3	0.6003289263830176
1103	4	0.685058686595255
1103	5	0.6713663462709576
1103	6	0.6526063239046138
1103	7	0.589587352203587
1103	8	0.77754334277612
1103	9	0.7896095078537115
1103	10	0.9240790479081511
1103	11	1.113245043555736
1103	12	1.037231874675605
1103	13	1.0037809655542418
1103	14	1.1053069069456356
1103	15	1.009303834398949
1103	16	0.9618983529721206
1103	17	1.0496129077092249
1103	18	1.0600132199813004
1103	19	1.099598659941492
1103	20	1.0047484862614056
1103	21	1.1389958194410355
1103	22	1.00891988127578
1103	23	1.1721730708615823
1103	24	1.2126476818261054
1103	25	1.2068235428095875
1104	1	0.3489003999638882
1104	2	0.2939998956059675
1104	3	0.27541482194572353
1104	4	0.30707296748106927
1104	5	0.3454169707603114
1104	6	0.39996159221015404
1104	7	0.25908837283715513
1104	8	0.38097064891562127
1104	9	0.47087908552165914
1104	10	0.514299329108006
1104	11	0.6048244839770831
1104	12	0.5227407410138625
1104	13	0.6117073096102175
1104	14	0.6014532500967462
1104	15	0.7951091768235017
1104	16	0.7626858509428303
1104	17	0.8006741270935436
1104	18	0.8274072377645467
1104	19	0.7766712646462537
1104	20	0.6116941172311812
1104	21	0.6842523351284484
1104	22	0.7055468669869143
1104	23	0.8993511986975946
1104	24	0.8701459452852944
1104	25	0.8180805854473192
1105	1	0.2011490274673946
1105	2	0.29536766971459727
1105	3	0.3406760212938629
1105	4	0.3278201173077093
1105	5	0.439169170961911
1105	6	0.47854592454233824
1105	7	0.3677113600283306
1105	8	0.35898194142351514
1105	9	0.48855067548822717
1105	10	0.5408322578618296
1105	11	0.5829164863193839
1105	12	0.6316092921693013
1105	13	0.5481049549437245
1105	14	0.7461556798062077
1105	15	0.7341303210828869
1105	16	0.7533904821418176
1105	17	0.8059730711684949
1105	18	0.6454572194760431
1105	19	0.7953367017758666
1105	20	0.8254511085059377
1105	21	0.7362930060160693
1105	22	0.9164798726726602
1105	23	0.9093708094701256
1105	24	0.8217775718632154
1105	25	0.8817314518759787
1106	1	0.34564540512572606
1106	2	0.322279832102339
1106	3	0.3304500005279962
1106	4	0.4658463584187871
1106	5	0.4894162246794025
1106	6	0.44987266977653917
1106	7	0.5438973848004736
1106	8	0.5733411041117265
1106	9	0.65091838489964
1106	10	0.610296084523597
1106	11	0.7434463163864891
1106	12	0.8168545096767303
1106	13	0.6904162426277622
1106	14	0.9373518871977851
1106	15	0.799280723379816
1106	16	0.840146399746061
1106	17	0.7753334023540219
1106	18	0.8552642504912953
1106	19	0.8578496387103982
1106	20	0.8972218827188669
1106	21	0.8435076422795476
1106	22	1.0551738265337107
1106	23	0.897792670385563
1106	24	1.0221464831575773
1106	25	0.9750226080206019
1107	1	0.26808732591894824
1107	2	0.21783361250076183
1107	3	0.25700090681891297
1107	4	0.17381850675238297
1107	5	0.26526342757163235
1107	6	0.20913775472330087
1107	7	0.19190856829041536
1107	8	0.20638304631663829
1107	9	0.17866752225562266
1107	10	0.2690559359594147
1107	11	0.49142414705296744
1107	12	0.44748475451115866
1107	13	0.468470084790674
1107	14	0.4171573702372626
1107	15	0.500539483643017
1107	16	0.4603623283957248
1107	17	0.4001347265846391
1107	18	0.3276798184363514
1107	19	0.3975613220809322
1107	20	0.4680451621661206
1107	21	0.4068969479643556
1107	22	0.3909819942695876
1107	23	0.4795371465815279
1107	24	0.500640500237516
1107	25	0.6898308498738714
1108	1	0.06267647532135356
1108	2	0.22690748293252128
1108	3	0.1606900136793996
1108	4	0.23804757492379736
1108	5	0.19450417470406478
1108	6	0.20812517676322528
1108	7	0.05632546305133701
1108	8	-0.08289759389911922
1108	9	0.051164973753820675
1108	10	0.050983245463925186
1108	11	0.18840768413267028
1108	12	-0.1395403612751025
1108	13	-0.03622357325255621
1108	14	-0.07641599049414083
1108	15	-0.13358957088288292
1108	16	0.12844986842927852
1108	17	0.1422247388656075
1108	18	0.17827099058664686
1108	19	0.1283082939791811
1108	20	0.18539383734651693
1108	21	0.1295424935042746
1108	22	0.1662374464580907
1108	23	0.19212112673933035
1108	24	0.23168125218601432
1108	25	0.0880589387784525
1109	1	0.08754090799683922
1109	2	0.061779356967715415
1109	3	0.19680356566204793
1109	4	0.04615588628929146
1109	5	0.18008693608182114
1109	6	0.2881043567887147
1109	7	0.23383711986215872
1109	8	0.17649039384638598
1109	9	0.348150827254031
1109	10	0.2273201174012911
1109	11	0.3717968276638288
1109	12	0.26153665679790095
1109	13	0.3823430150423448
1109	14	0.32780777277562834
1109	15	0.44085094177930273
1109	16	0.35044423199511243
1109	17	0.43996145722597646
1109	18	0.27943073441387867
1109	19	0.3551941641037075
1109	20	0.37737181408831333
1109	21	0.3616935214410404
1109	22	0.3901230236780293
1109	23	0.522359566903674
1109	24	0.3684696721636911
1109	25	0.3057175228618618
1110	1	-0.20452713546373857
1110	2	0.005243166216885697
1110	3	-0.13734390438476396
1110	4	-0.07769407749874802
1110	5	-0.06578585388919578
1110	6	-0.16485025508951168
1110	7	-0.17536776200045523
1110	8	-0.2968117983684877
1110	9	-0.10418267458847907
1110	10	-0.10154052317486872
1110	11	-0.2631870005144066
1110	12	-0.10260661414034189
1110	13	-0.21053414488142863
1110	14	-0.08903995369311701
1110	15	-0.07957194231167364
1110	16	0.020277158843839516
1110	17	-0.01988879921541553
1110	18	-0.18237586297333053
1110	19	-0.05060161756288295
1110	20	-0.12180607128108534
1110	21	-0.09789482581862785
1110	22	-0.20327056044209968
1110	23	-0.2710372862166395
1110	24	-0.05788850985190663
1110	25	-0.07556815156206653
1111	1	-0.20760919525878307
1111	2	-0.12974669122327853
1111	3	-0.10883080929662015
1111	4	-0.047613444618058054
1111	5	-0.03189071611394212
1111	6	-0.04862460334309304
1111	7	0.04099982918388889
1111	8	0.0017614752813486234
1111	9	0.09358915823506209
1111	10	-0.015793686732337164
1111	11	0.20084028193755188
1111	12	0.03721783215485175
1111	13	0.09516768453932656
1111	14	0.10598295401616298
1111	15	-0.0317998274892517
1111	16	0.09119987798987239
1111	17	0.008628807854861975
1111	18	0.08495193440858628
1111	19	0.007884950830302273
1111	20	-0.046345426105240506
1111	21	0.025796652363119676
1111	22	-0.018387204580193384
1111	23	-0.20085566013587908
1111	24	0.01795414981116039
1111	25	-0.04055592221049409
1112	1	-0.0902224614475351
1112	2	-0.09553554753876625
1112	3	-0.11305110417642794
1112	4	-0.09450553035633646
1112	5	-0.22020548141532714
1112	6	-0.0793400262411339
1112	7	-0.2057092326484664
1112	8	-0.3229540944656293
1112	9	-0.3204320488989296
1112	10	-0.2980323090745074
1112	11	-0.2497853482144663
1112	12	-0.11840442226269943
1112	13	-0.24201356822528197
1112	14	-0.19300862366064564
1112	15	-0.11072897646390345
1112	16	-0.1559952179723183
1112	17	-0.15445050668299842
1112	18	-0.37578872143304665
1112	19	-0.1920803068626995
1112	20	0.07033714919513301
1112	21	-0.12209024531004076
1112	22	-0.2526857412843597
1112	23	-0.4686755087382224
1112	24	-0.3504002647003759
1112	25	-0.2935633703164129
1113	1	-0.12285229270059261
1113	2	-0.0556758988569257
1113	3	-0.05978679447911617
1113	4	0.02704058233386064
1113	5	-0.08431528527118815
1113	6	-0.2528929980068
1113	7	-0.09740789413731221
1113	8	-0.26646202562072574
1113	9	-0.2123684460550379
1113	10	-0.23214880862801124
1113	11	0.04880785708372315
1113	12	-0.09716809904552548
1113	13	-0.08420630796651807
1113	14	-0.05385855136247386
1113	15	0.14133401755325536
1113	16	0.12074236444097153
1113	17	0.06930797901410557
1113	18	0.017711285199816018
1113	19	-0.05626551586411921
1113	20	-0.008060114435920696
1113	21	0.09915752831813052
1113	22	0.2098546580696663
1113	23	-0.1908976145944763
1113	24	-0.06878019911186328
1113	25	0.07582339733729526
1114	1	-0.7452111371759003
1114	2	-0.6982516595274184
1114	3	-0.5572949135174952
1114	4	-0.5726680173788239
1114	5	-0.6132224661095762
1114	6	-0.7258874082956766
1114	7	-0.5042108653308972
1114	8	-0.6503996060451627
1114	9	-0.9618847079612607
1114	10	-0.7883414051543554
1114	11	-0.3993657848071166
1114	12	-0.5082862455218304
1114	13	-0.46633625343108775
1114	14	-0.7181844839455209
1114	15	-0.666581449129751
1114	16	-0.614496645070389
1114	17	-0.6943085164208469
1114	18	-0.662703619788708
1114	19	-0.9032531313519101
1114	20	-0.7990321522634929
1114	21	-0.6429423239392023
1114	22	-0.8870039378983918
1114	23	-0.879990529073936
1114	24	-0.8639469032248286
1114	25	-0.8863429050790117
1115	1	-0.9417848910834081
1115	2	-0.9079696033243607
1115	3	-1.0420503466206341
1115	4	-0.9230891011694595
1115	5	-0.9969620284686869
1115	6	-1.0101409238330064
1115	7	-1.0671912733056317
1115	8	-1.1306423350714851
1115	9	-1.179014962563251
1115	10	-1.2736133937302796
1115	11	-1.1705909125635117
1115	12	-1.3424114354080103
1115	13	-1.5186235077175567
1115	14	-1.4622614988534153
1115	15	-1.4037526248139116
1115	16	-1.7120888720165013
1115	17	-1.6676867176497794
1115	18	-1.4718305174307034
1115	19	-1.5215827184653463
1115	20	-1.5806921559717608
1115	21	-1.7214106133533917
1115	22	-1.6077569118331567
1115	23	-1.4209434893466621
1115	24	-1.3954619916540523
1115	25	-1.4131374311523786
1116	1	-1.2151292895444143
1116	2	-1.039798673989658
1116	3	-1.051896349019028
1116	4	-1.160760957817537
1116	5	-1.1485501653105956
1116	6	-1.416339925947021
1116	7	-1.366402068416349
1116	8	-1.4572499181319074
1116	9	-1.4364183649160296
1116	10	-1.6033592595701975
1116	11	-1.6992699330751222
1116	12	-1.5920385646463657
1116	13	-1.4723258494566522
1116	14	-1.8243404395872886
1116	15	-1.8192336178158044
1116	16	-1.7959953112691736
1116	17	-1.8444880353426285
1116	18	-1.8449492796547773
1116	19	-2.0487186945864337
1116	20	-2.1605845604164102
1116	21	-2.241491226193183
1116	22	-2.219561359584276
1116	23	-2.2582737758157947
1116	24	-2.2597180547777924
1116	25	-2.2652980887081853
1201	1	0.9024364228729205
1201	2	0.9349857185134027
1201	3	0.8712452610443719
1201	4	0.755443812757278
1201	5	0.9475947659256718
1201	6	0.9540671472777902
1201	7	0.9620973123629497
1201	8	1.042483581819944
1201	9	1.197976041718249
1201	10	1.177731106341092
1201	11	1.0242676199169551
1201	12	1.0319197896822487
1201	13	1.1651355472275498
1201	14	1.1969403730810981
1201	15	1.1945627985423712
1201	16	1.2799594419495435
1201	17	1.4188028147477354
1201	18	1.5022442956785156
1201	19	1.203184317312008
1201	20	1.2431283003384337
1201	21	1.2943743054968095
1201	22	1.3342850871190777
1201	23	1.2060376126942458
1201	24	1.351425902277633
1201	25	1.25463230774983
1202	1	0.6126316898690227
1202	2	0.6404859250747847
1202	3	0.5243630967775417
1202	4	0.5970333956805902
1202	5	0.6373528674758617
1202	6	0.5863940922792708
1202	7	0.6292657547482818
1202	8	0.6342890346858283
1202	9	0.6764129837587234
1202	10	0.7131665785155832
1202	11	0.6088061901575799
1202	12	0.5001049041420771
1202	13	0.4829031949278004
1202	14	0.5840080347885177
1202	15	0.547265915757535
1202	16	0.5389812097958284
1202	17	0.6503182306098765
1202	18	0.6830892292508537
1202	19	0.6316874831089905
1202	20	0.6363892574002605
1202	21	0.6081392723317407
1202	22	0.6260986960567081
1202	23	0.6095477377481018
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2210	21	0.3379056433283836
2210	22	0.4879112791048925
2210	23	0.4085647961220573
2210	24	0.40458933721563284
2210	25	0.47653724540222697
2211	1	0.08303974443968443
2211	2	0.16150575315189286
2211	3	0.13874275756100474
2211	4	-0.0016716964118046462
2211	5	0.1302972692497093
2211	6	0.22713643780215165
2211	7	0.19272489202630538
2211	8	-0.06410426395751045
2211	9	0.044521871994426476
2211	10	0.12709701005277907
2211	11	0.01453132016495573
2211	12	0.115156927115315
2211	13	0.174841175822948
2211	14	0.287956831144065
2211	15	0.19597008680583627
2211	16	0.1942251943767488
2211	17	0.05764317416588938
2211	18	0.2197143474848886
2211	19	0.126059503231005
2211	20	0.09511607011515366
2211	21	0.1755825260040993
2211	22	0.18948735343872158
2211	23	0.19848175334819018
2211	24	-0.054649456355818415
2211	25	0.12715023444336992
2212	1	-0.21274105225896278
2212	2	-0.235042868767799
2212	3	-0.07231461847115384
2212	4	-0.09472768745284554
2212	5	-0.10656025023047988
2212	6	-0.212780293053207
2212	7	-0.11383819134994866
2212	8	-0.06087473897270712
2212	9	-0.031209357717202124
2212	10	-0.11055162993048384
2212	11	0.010787460804827731
2212	12	-0.10465862656828762
2212	13	-0.302678523537125
2212	14	-0.1348970777441636
2212	15	-0.09342412627847807
2212	16	-0.1859944599834833
2212	17	-0.17376699636334791
2212	18	-0.13174146624335492
2212	19	-0.22635816880323745
2212	20	-0.06558107743235553
2212	21	-0.0296390107138933
2212	22	-0.1687442412558937
2212	23	-0.14137767606233353
2212	24	-0.10939160162152461
2212	25	-0.06502170241262917
2213	1	-0.586915218739243
2213	2	-0.7186941175672636
2213	3	-0.6285066127764445
2213	4	-0.6629928986813383
2213	5	-0.7564663885945109
2213	6	-0.7871380281037261
2213	7	-0.7798233715289768
2213	8	-0.7491450466352916
2213	9	-0.8161921191673898
2213	10	-0.8023025389445948
2213	11	-0.8324597986500564
2213	12	-0.9051318470922354
2213	13	-0.9455521368188755
2213	14	-0.8699046635370209
2213	15	-0.8748009528757734
2213	16	-1.0084412053632832
2213	17	-0.7932108618424181
2213	18	-0.9855288576931116
2213	19	-0.7333578024970393
2213	20	-1.0336463090188914
2213	21	-1.0211298354014104
2213	22	-1.000527640037319
2213	23	-0.8766445844864776
2213	24	-0.9309011155421985
2213	25	-1.0181504351524353
2214	1	-0.47747251281290914
2214	2	-0.7035079259563375
2214	3	-0.5371212728201691
2214	4	-0.5223023454669686
2214	5	-0.5156000737631885
2214	6	-0.6980861443177346
2214	7	-0.7040950610007357
2214	8	-0.5595843369810396
2214	9	-0.7133311106247575
2214	10	-0.8346953781969475
2214	11	-0.8403690714129581
2214	12	-0.7437394780088908
2214	13	-0.6054962114520208
2214	14	-0.5701816151564287
2214	15	-0.717692940406593
2214	16	-0.7355973783881495
2214	17	-0.6891463410101863
2214	18	-0.6261943307680795
2214	19	-0.6372634155660037
2214	20	-0.5452420278725327
2214	21	-0.6831441919420058
2214	22	-0.9207898512227928
2214	23	-0.9302742825817916
2214	24	-0.8780371977033994
2214	25	-0.7933661745116041
2215	1	-0.8659633039858186
2215	2	-0.9661822166321272
2215	3	-0.838661988504537
2215	4	-0.945712967612728
2215	5	-0.8947686596757407
2215	6	-0.9053110795048269
2215	7	-0.8809142961406025
2215	8	-0.8665891699275079
2215	9	-0.7346875528629369
2215	10	-0.9145701389237857
2215	11	-1.003791916436306
2215	12	-1.0281946522850305
2215	13	-1.14956931097867
2215	14	-1.2136586232905913
2215	15	-1.1693285901216655
2215	16	-1.198171922113687
2215	17	-1.3223566487980065
2215	18	-1.3592669050727721
2215	19	-1.3380913669354868
2215	20	-1.4755761478544187
2215	21	-1.5101872439919575
2215	22	-1.577960746044674
2215	23	-1.7188553240717397
2215	24	-1.407756814452874
2215	25	-1.568385119325825
2216	1	-1.2110292226500725
2216	2	-1.2747351149405581
2216	3	-1.3232667969283227
2216	4	-1.1745756287611684
2216	5	-1.3787684972504266
2216	6	-1.3017944445512768
2216	7	-1.3834439435190546
2216	8	-1.3029225707265653
2216	9	-1.679028331324325
2216	10	-1.3997773803011242
2216	11	-1.3640620500374752
2216	12	-1.5198960511096047
2216	13	-1.5079017613358516
2216	14	-1.7248834180224222
2216	15	-1.7563765036860133
2216	16	-1.9600583144888333
2216	17	-1.932738291184414
2216	18	-2.0719812541438074
2216	19	-1.9501178828680779
2216	20	-1.9438959150786417
2216	21	-2.1302090825830646
2216	22	-2.0853425324566572
2216	23	-2.1373568363402455
2216	24	-2.3826443380949556
2216	25	-2.667448699319344
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24365.0
3	2.0
4	197.0
5	1393.0
6	2707.0
7	2668.0
8	2392.0
9	2050.0
10	1931.0
11	2688.0
12	2856.0
13	2505.0
14	1276.0
15	1147.0
16	1206.0
17	1325.0
18	1576.0
19	1993.0
20	2792.0
21	3381.0
22	4119.0
23	4883.0
24	5529.0
25	6276.0
26	6904.0
27	7666.0
28	8880.0
29	10531.0
30	12996.0
31	17014.0
32	22049.0
33	29625.0
34	41047.0
35	58416.0
36	90223.0
37	170287.0
38	481497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.93048644247337	14.501141290401003	13.112876804309698	23.45549546281593
2	11.043929898647022	21.67626382983642	39.89639302068865	27.38341325082791
3	20.772792822126256	30.560101880492716	27.10525884831501	21.561846449066017
4	13.388943162746425	15.077470311557633	31.89289230846306	39.64069421723288
5	16.957739314893114	33.28738209944155	29.857223639934993	19.897654945730338
6	37.47946240403341	29.699122412262906	14.405660040993281	18.415755142710395
7	25.791734849054315	32.3741541047243	22.494212929500858	19.339898116720537
8	26.760927159338525	33.82574176891177	22.8715494385715	16.541781633178203
9	29.80874854017553	18.75888903021403	15.176296690293661	36.25606573931678
10	14.766287245952878	25.517343708148587	29.708507001651412	30.007862044247126
11	31.18010845423642	26.165844280182487	17.51392501230673	25.140122253274367
12	27.43096239171568	23.701411254790848	25.945242461978236	22.922383891515235
13	32.28756334980711	19.21564830994574	26.244608913263406	22.25217942698375
14	30.61216192670319	20.08082783187811	22.68435521654115	26.622655024877552
15	35.12962206392326	19.24145582548992	24.670402332790538	20.95851977779628
16	25.93000183900036	21.762827027497895	31.9126571619385	20.394513971563246
17	24.855947321196094	28.535158726548186	27.204540768868167	19.404353183387553
18	28.049709473121787	18.36470584819039	35.17286905818571	18.41271562050211
19	24.372502400793035	32.56588627985502	20.40867693070227	22.65293438864967
20	20.997416485898118	27.856167280844147	28.207443889151858	22.93897234410588
21	21.923205374558936	32.50010890451736	21.618853550045255	23.957832170878447
22	20.71675665002507	28.83026150990075	18.32350259496648	32.1294792451077
23	23.808509711486803	24.759378735885313	19.906297341380665	31.525814211247223
24	19.249500596187495	29.975746008640847	20.187334499899347	30.58741889527231
25	26.0118040580563	27.1947263076798	19.714499750291324	27.078969883972576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	422.0
1	422.0
2	593.5
3	765.0
4	765.0
5	765.0
6	1804.0
7	2843.0
8	2843.0
9	2843.0
10	2636.5
11	2430.0
12	2430.0
13	2430.0
14	2782.0
15	3134.0
16	3134.0
17	3134.0
18	4852.0
19	6570.0
20	6570.0
21	6570.0
22	8578.5
23	10587.0
24	10587.0
25	10587.0
26	14897.0
27	19207.0
28	19207.0
29	19207.0
30	22767.0
31	26327.0
32	26327.0
33	26327.0
34	31957.0
35	37587.0
36	37587.0
37	37587.0
38	41993.0
39	46399.0
40	46399.0
41	46399.0
42	57064.0
43	67729.0
44	67729.0
45	67729.0
46	136239.0
47	204749.0
48	204749.0
49	204749.0
50	285933.5
51	367118.0
52	367118.0
53	367118.0
54	232699.0
55	98280.0
56	98280.0
57	98280.0
58	73327.5
59	48375.0
60	48375.0
61	48375.0
62	42581.5
63	36788.0
64	36788.0
65	36788.0
66	30606.0
67	24424.0
68	24424.0
69	24424.0
70	18972.5
71	13521.0
72	13521.0
73	13521.0
74	11213.5
75	8906.0
76	8906.0
77	8906.0
78	7850.5
79	6795.0
80	6795.0
81	6795.0
82	5007.0
83	3219.0
84	3219.0
85	3219.0
86	2384.0
87	1549.0
88	1549.0
89	1549.0
90	1075.5
91	602.0
92	602.0
93	602.0
94	324.5
95	47.0
96	47.0
97	47.0
98	33.0
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17777486729481737
2	0.02292005331319964
3	0.03014275918920793
4	0.04295102427599596
5	0.032261419579503695
6	0.0636561144538864
7	0.0706862148398678
8	0.07424941640536521
9	0.057396436028012546
10	0.04757355603664127
11	0.6198044669065248
12	0.5483478301065494
13	0.5235980246380942
14	0.5134862364116827
15	0.5263908042434842
16	0.5028929344602039
17	0.506841347005755
18	0.5074191634758357
19	0.5200348230725969
20	0.5106934568062927
21	0.5178198599372876
22	0.5044337783804189
23	0.5139677501367499
24	0.4969221642693703
25	0.4996186411297468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1038392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.832707170788748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.62523793739406	27.32556009265149
2	6.821487791989629	4.206498711190531
3	1.4772720120868732	1.3664488608082936
4	0.6612377825668346	0.8155100368057957
5	0.3911801116167341	0.6030570916257609
6	0.26790625968977655	0.49561651525417
7	0.19544426587056069	0.42182530724577444
8	0.15882948267134311	0.39177143434347106
9	0.11417990598478099	0.31684280454152475
>10	0.9259852379805278	5.908276799865235
>50	0.157237920044908	3.437388597256135
>100	0.16351488182485305	10.120565339841098
>500	0.015692407084918716	3.3309439134110703
>1k	0.017889344076807333	11.675915450824077
>5k	0.004080025842078866	8.468834421881258
>10k+	0.0028246332752853687	21.114944622454317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	53030	5.106934568062928	No Hit
CTTCCGTACTCTGCGTTGATACCAC	34392	3.312044006502361	No Hit
GAGTACGGAAGCAGTGGTATCAACG	24778	2.386189415943112	No Hit
TCCGTACTCTGCGTTGATACCACTG	24146	2.3253260810946155	No Hit
GTACATGGGAAGCAGTGGTATCAAC	20781	2.001267344124377	No Hit
CCCATGTACTCTGCGTTGATACCAC	19873	1.9138244516521699	No Hit
GCTTCCGTACTCTGCGTTGATACCA	17266	1.6627631954021216	No Hit
GCGTTGATACCACTGCTTCCGTACT	12885	1.2408608694982242	No Hit
GTATCAACGCAGAGTACGGAAGCAG	11051	1.0642416351435682	No Hit
GTACATGGAAGCAGTGGTATCAACG	9264	0.892148629804544	No Hit
ACTCTGCGTTGATACCACTGCTTCC	9102	0.8765475851123661	No Hit
TATCAACGCAGAGTACGGAAGCAGT	8612	0.8293592400557785	No Hit
TCCATGTACTCTGCGTTGATACCAC	8292	0.7985423616514765	No Hit
CATGTACTCTGCGTTGATACCACTG	6590	0.634635089638595	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6443	0.6204785861216188	No Hit
GGTATCAACGCAGAGTACGGAAGCA	6045	0.5821500936062681	No Hit
ATACCACTGCTTCCGTACTCTGCGT	5891	0.5673194708741978	No Hit
CAGTGGTATCAACGCAGAGTACGGA	5857	0.5640451775437407	No Hit
GTATCAACGCAGAGTACATGGGAAG	5577	0.5370804089399764	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	5372	0.5173383462122204	No Hit
GCGTTGATACCACTGCTTCCCATGT	5371	0.5172420434672069	No Hit
GTACTCTGCGTTGATACCACTGCTT	5101	0.4912403023135771	No Hit
TATCAACGCAGAGTACATGGGAAGC	4621	0.4450149847071241	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4541	0.4373107651060486	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	4115	0.3962857957303215	No Hit
GTGGTATCAACGCAGAGTACGGAAG	4060	0.39098914475458213	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3486	0.33571136911686533	No Hit
GATACCACTGCTTCCGTACTCTGCG	3374	0.32492546167535963	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3358	0.3233846177551445	No Hit
GTTGATACCACTGCTTCCGTACTCT	3347	0.3223252875599966	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	3128	0.30123498640205243	No Hit
GTACGGGAAGCAGTGGTATCAACGC	3103	0.2988274177767163	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2917	0.28091510720421575	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	2916	0.28081880445920226	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2888	0.2781223275988259	No Hit
GGTATCAACGCAGAGTACATGGGAA	2857	0.27513694250340914	No Hit
CAGTGGTATCAACGCAGAGTACATG	2813	0.2708996217228176	No Hit
TTCCCGTACTCTGCGTTGATACCAC	2616	0.25192798095516916	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2508	0.24152728449371724	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2467	0.23757887194816601	No Hit
CCCGTACTCTGCGTTGATACCACTG	2445	0.23546021155787022	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	2221	0.21388839667485882	No Hit
ATACCACTGCTTCCCATGTACTCTG	2036	0.19607238884737171	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1983	0.1909683433616592	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1958	0.18856077473632307	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1954	0.18817556375626932	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1898	0.18278261003551646	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1860	0.1791231057250056	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1852	0.17835268376489802	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1850	0.17816007827487115	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1845	0.17767856454980394	No Hit
GCGTTGATACCACTGCTTCCATGTA	1785	0.1719003998489973	No Hit
TACCACTGCTTCCCATGTACTCTGC	1739	0.16747047357837888	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1729	0.16650744612824445	No Hit
GTGGTATCAACGCAGAGTACATGGG	1686	0.16236642809266635	No Hit
TGATACCACTGCTTCCGTACTCTGC	1680	0.16178861162258568	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1680	0.16178861162258568	No Hit
AAAGTACTCTGCGTTGATACCACTG	1678	0.1615960061325588	No Hit
CCATGTACTCTGCGTTGATACCACT	1613	0.15533632770668496	No Hit
GTATCAACGCAGAGTACATGGAAGC	1610	0.1550474194716446	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1594	0.15350657555142952	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1489	0.1433947873250179	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1459	0.1405057049746146	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1446	0.13925376928943983	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1446	0.13925376928943983	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1438	0.13848334732933226	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1334	0.1284678618479341	No Hit
TATCAACGCAGAGTACATGGAAGCA	1319	0.12702332067273245	No Hit
GTTGATACCACTGCTTCCCATGTAC	1311	0.1262528987126249	No Hit
CTTCCCATGTACTCTGCGTTGATAC	1298	0.12500096302745012	No Hit
TATCAACGCAGAGTACGGGAAGCAG	1280	0.12326751361720815	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1223	0.11777825715144186	No Hit
GATACCACTGCTTCCCATGTACTCT	1217	0.11720044068136118	No Hit
GCTTCCCGTACTCTGCGTTGATACC	1170	0.11267421166572933	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1148	0.11055555127543355	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	1092	0.1051625975546807	No Hit
ATCAACGCAGAGTACATGGGAAGCA	1091	0.10506629480966725	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1074	0.10342914814443871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.778164700806632E-4	0.0
10	0.0	0.0	0.0	8.667247051209947E-4	0.0
11	0.0	0.0	0.0	0.0012519356851747702	0.0
12	0.0	0.0	0.0	0.0016371466652285458	0.0
13	0.0	0.0	0.0	0.0025038713703495405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATT	25	0.0060105375	19.01104	13
CGAACGA	70	2.4836481E-8	16.29439	16
TCTAGGA	50	9.0272166E-5	15.1362705	2
AACGAAC	70	4.4027183E-7	14.937246	14
TTAGCCT	45	6.97841E-4	14.715817	4
CGGACCA	60	2.5305293E-5	14.271412	9
TCCGATA	80	1.2635792E-7	14.271411	8
CCGATAA	80	1.2635792E-7	14.271411	9
ACGAACG	80	1.2778764E-7	14.25828	15
CCTTACT	40	0.0054266923	14.190252	2
CTGTAGA	95	5.0695235E-9	13.964855	1
GATAACG	75	9.5014184E-7	13.9542675	11
CGACCAA	55	1.9495201E-4	13.826212	14
TTCCGAT	85	2.6472117E-7	13.431916	7
GGGTAGG	115	3.9108272E-10	13.184213	1
CGAGCGA	65	5.3728047E-5	13.17361	10
TTAACCA	65	5.6681303E-5	13.098695	4
CGATAAC	80	1.9641557E-6	13.082127	10
CGCCAGT	80	1.9845647E-6	13.070089	18
AACCGCG	95	7.233757E-8	13.019532	7
>>END_MODULE