FastQCFastQC Report
Thu 2 Feb 2017
SRR4050252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038392
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA464264.470951239994145No Hit
CTTCCGTACTCTGCGTTGATACCAC328953.1678787972172358No Hit
TCCGTACTCTGCGTTGATACCACTG247792.3862857186881254No Hit
GAGTACGGAAGCAGTGGTATCAACG247462.383107728102682No Hit
CCCATGTACTCTGCGTTGATACCAC211592.0376697817394587No Hit
GTACATGGGAAGCAGTGGTATCAAC170991.6466806369848768No Hit
GCTTCCGTACTCTGCGTTGATACCA167331.6114338323099564No Hit
GCGTTGATACCACTGCTTCCGTACT127691.2296897510766647No Hit
GTATCAACGCAGAGTACGGAAGCAG110181.0610636445581245No Hit
TATCAACGCAGAGTACGGAAGCAGT100210.965049807779721No Hit
ACTCTGCGTTGATACCACTGCTTCC95470.9194023066433487No Hit
TCCATGTACTCTGCGTTGATACCAC93390.8993713356805523No Hit
GTACATGGAAGCAGTGGTATCAACG76100.7328638895523077No Hit
CATGTACTCTGCGTTGATACCACTG71900.6924167366466614No Hit
CAGTGGTATCAACGCAGAGTACGGA65510.6308792825830707No Hit
ACGCAGAGTACGGAAGCAGTGGTAT63780.6142189076957449No Hit
GGTATCAACGCAGAGTACGGAAGCA63720.6136410912256642No Hit
ATACCACTGCTTCCGTACTCTGCGT61250.5898543132073436No Hit
GAGTACATGGGAAGCAGTGGTATCA61040.5878319555620614No Hit
GCGTTGATACCACTGCTTCCCATGT56940.5483478301065494No Hit
AAGCAGTGGTATCAACGCAGAGTAC53110.5114638787664004No Hit
GTATCAACGCAGAGTACATGGGAAG51830.4991371274046795No Hit
TATCAACGCAGAGTACATGGGAAGC49340.47515774389633203No Hit
GAAGCAGTGGTATCAACGCAGAGTA46600.4487707917626484No Hit
GTGGTATCAACGCAGAGTACGGAAG45950.44251111333677456No Hit
GTACTCTGCGTTGATACCACTGCTT42180.4062049784667062No Hit
ACGCAGAGTACATGGGAAGCAGTGG39150.37702524672763277No Hit
ATCAACGCAGAGTACGGAAGCAGTG37840.36440958713087157No Hit
GCTTCCCATGTACTCTGCGTTGATA34830.335422460881825No Hit
GATACCACTGCTTCCGTACTCTGCG34120.32858496598587045No Hit
GTTGATACCACTGCTTCCGTACTCT32910.3169323338392438No Hit
AAAAAGTACTCTGCGTTGATACCAC32110.30922811423816826No Hit
CCACTGCTTCCGTACTCTGCGTTGA31660.3048944907125633No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30940.29796069307159534No Hit
CAGTGGTATCAACGCAGAGTACATG30310.2918936201357484No Hit
CGGAAGCAGTGGTATCAACGCAGAG27820.2679142366274008No Hit
GTACGGGAAGCAGTGGTATCAACGC27690.26666230094222604No Hit
TTCCCGTACTCTGCGTTGATACCAC27050.26049892526136564No Hit
GTATCAACGCAGAGTACTTTTTTTT26900.259054384086164No Hit
GGTATCAACGCAGAGTACATGGGAA26710.25722463193090855No Hit
GAGTACATGGAAGCAGTGGTATCAA26570.25587639350072033No Hit
CCCGTACTCTGCGTTGATACCACTG26510.2552985770306397No Hit
CTGCTTCCGTACTCTGCGTTGATAC24700.23786778018320634No Hit
GCAGAGTACGGAAGCAGTGGTATCA24380.23478609234277614No Hit
CATGGAAGCAGTGGTATCAACGCAG22430.2160070570651546No Hit
ATACCACTGCTTCCCATGTACTCTG22030.2121549472646168No Hit
TATCAACGCAGAGTACTTTTTTTTT21150.20368030570343376No Hit
CTGCTTCCCATGTACTCTGCGTTGA21040.2026209755082859No Hit
GGGAAGCAGTGGTATCAACGCAGAG20210.19462784767217003No Hit
CACTGCTTCCGTACTCTGCGTTGAT19850.19116094885168608No Hit
GTATCAACGCAGAGTACGGGAAGCA19550.18827186650128275No Hit
GCAGTGGTATCAACGCAGAGTACGG19040.1833604265055971No Hit
TACCACTGCTTCCCATGTACTCTGC18440.17758226180479048No Hit
GCGTTGATACCACTGCTTCCATGTA18420.1773896563147636No Hit
GTGGTATCAACGCAGAGTACATGGG18140.17469317945438717No Hit
AAAGTACTCTGCGTTGATACCACTG17990.17324863827918552No Hit
GCTTCCATGTACTCTGCGTTGATAC17810.1715151888689435No Hit
GAGTACGGGAAGCAGTGGTATCAAC17800.17141888612393008No Hit
CCATGTACTCTGCGTTGATACCACT17660.17007064769374186No Hit
CTTCCCGTACTCTGCGTTGATACCA17160.1652555104430697No Hit
TGATACCACTGCTTCCGTACTCTGC16840.16217382260263946No Hit
GCAGAGTACATGGGAAGCAGTGGTA15880.15292875908134884No Hit
TACCACTGCTTCCGTACTCTGCGTT15700.15119530967110686No Hit
CATGGGAAGCAGTGGTATCAACGCA15620.1504248877109993No Hit
TATCAACGCAGAGTACGGGAAGCAG15320.147535805360596No Hit
GTATCAACGCAGAGTACATGGAAGC15120.14560975046032712No Hit
GCGTTGATACCACTGCTTCCCGTAC15030.14474302575520612No Hit
CTGCGTTGATACCACTGCTTCCGTA15030.14474302575520612No Hit
GCAGTGGTATCAACGCAGAGTACAT14800.14252806261989692No Hit
TCAACGCAGAGTACGGAAGCAGTGG13990.13472754027380796No Hit
TATCAACGCAGAGTACATGGAAGCA13710.13203106341343152No Hit
CCACTGCTTCCCATGTACTCTGCGT13390.12894937557300135No Hit
GATACCACTGCTTCCCATGTACTCT13360.12866046733796102No Hit
TACGGAAGCAGTGGTATCAACGCAG13190.12702332067273245No Hit
GTTGATACCACTGCTTCCCATGTAC12910.12432684381235602No Hit
ATCAACGCAGAGTACATGGGAAGCA12220.1176819544064284No Hit
CTTCCCATGTACTCTGCGTTGATAC12070.11623741323122673No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11970.11527438578109231No Hit
GCTTCCCGTACTCTGCGTTGATACC11780.11344463362583687No Hit
ACGCAGAGTACATGGAAGCAGTGGT11430.11007403755036634No Hit
GGTATCAACGCAGAGTACTTTTTTT11120.10708865245494957No Hit
TCCCATGTACTCTGCGTTGATACCA11080.1067034414748958No Hit
CTCTGCGTTGATACCACTGCTTCCG10560.10169569873419672No Hit
ACATGGGAAGCAGTGGTATCAACGC10540.10150309324416984No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10510.10121418500912949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA250.00590234719.070921
TGTCCAA250.006057401718.98550410
CGCATCC704.470312E-714.91718211
TAGGACT456.6683895E-414.8042914
GTTATCA602.4780102E-514.303191
GGACGGA1300.013.9364421
CGAACCC759.747328E-713.92270310
ACGCATC759.747328E-713.92270310
CGTCGTA1500.013.92270310
CAGAACC551.9295952E-413.8429734
GTCGTAG1550.013.47358411
ATCGAAC852.719662E-713.4021778
TAAACTG500.001498875213.3001025
CCGTCGT1650.013.2323219
AATCGAA802.0093285E-613.0556777
CCGACCA1251.03682396E-1012.9101429
AAAAAGT5100.012.9009171
GTATTAG905.160582E-712.7139461
ACCGTCG1650.012.6576128
GACCATA1352.7284841E-1112.657001511
Produced by FastQC (version 0.11.5)