##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050252_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1038392 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.999818950839376 31.0 31.0 34.0 28.0 34.0 2 30.777310495458362 31.0 31.0 34.0 27.0 34.0 3 31.151169307929955 31.0 31.0 34.0 27.0 34.0 4 34.72442969514403 37.0 35.0 37.0 32.0 37.0 5 34.63638298446059 37.0 35.0 37.0 32.0 37.0 6 34.67242717586422 37.0 35.0 37.0 32.0 37.0 7 34.53515820614951 37.0 35.0 37.0 32.0 37.0 8 34.566755136788416 37.0 35.0 37.0 32.0 37.0 9 36.165302698788125 39.0 37.0 39.0 32.0 39.0 10 36.108011232752176 39.0 37.0 39.0 32.0 39.0 11 36.116641884760284 39.0 37.0 39.0 32.0 39.0 12 36.060486791115494 39.0 37.0 39.0 32.0 39.0 13 35.97949618255919 39.0 37.0 39.0 31.0 39.0 14 37.18130628895446 40.0 37.0 41.0 32.0 41.0 15 37.1529143136696 40.0 37.0 41.0 32.0 41.0 16 37.00459460396459 40.0 37.0 41.0 31.0 41.0 17 37.03445808519326 40.0 37.0 41.0 31.0 41.0 18 36.96331539534203 40.0 37.0 41.0 31.0 41.0 19 37.04822263653803 40.0 37.0 41.0 31.0 41.0 20 37.027898905230394 40.0 37.0 41.0 31.0 41.0 21 36.981789150917955 40.0 37.0 41.0 31.0 41.0 22 36.95078351913343 40.0 37.0 41.0 31.0 41.0 23 36.86447796207983 40.0 37.0 41.0 31.0 41.0 24 36.7825840337753 40.0 37.0 41.0 31.0 41.0 25 36.56807159531275 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5233090629122934 1101 2 0.8934502464479444 1101 3 0.7103646862783641 1101 4 0.6592204422125079 1101 5 0.8029997378334102 1101 6 0.8587700174759334 1101 7 0.758525624578219 1101 8 0.7275303874803711 1101 9 0.9200640651581722 1101 10 0.7969584479644922 1101 11 0.7956667587853588 1101 12 0.8543991641115269 1101 13 0.9888953306647466 1101 14 0.942211594499895 1101 15 0.877989670984725 1101 16 0.9080509137734509 1101 17 0.8609679559967134 1101 18 0.7798820065578127 1101 19 1.0185773273212888 1101 20 0.9803298916525449 1101 21 0.7277453405453258 1101 22 0.8537110862914332 1101 23 0.9851215053625708 1101 24 1.1509568316364707 1101 25 1.01115183657474 1102 1 0.46466662479420506 1102 2 0.7657685140469859 1102 3 0.5123105652063309 1102 4 0.49533852255659383 1102 5 0.4364984431409198 1102 6 0.32130256765440635 1102 7 0.2399162486638886 1102 8 0.3292039786793879 1102 9 0.43416853294446867 1102 10 0.45483687121921434 1102 11 0.678351709630931 1102 12 0.4340373816783867 1102 13 0.5134094552478103 1102 14 0.5035686809297673 1102 15 0.5464414162594693 1102 16 0.5861369632789746 1102 17 0.5858842032902984 1102 18 0.45391939903250744 1102 19 0.47832116838863925 1102 20 0.406164858590337 1102 21 0.3578628503469474 1102 22 0.5370728248366987 1102 23 0.6233637591515588 1102 24 0.5392685745705208 1102 25 0.33569265961880035 1103 1 0.45560053408458145 1103 2 0.7703687724337271 1103 3 0.5717151569841477 1103 4 0.5654376550122748 1103 5 0.6666782673224532 1103 6 0.5990015543164589 1103 7 0.6326415431381633 1103 8 0.6294811309399151 1103 9 0.735125935219429 1103 10 0.592351125795112 1103 11 0.6196923254286091 1103 12 0.5789035691026001 1103 13 0.7882836901920882 1103 14 0.9134526955957369 1103 15 0.8697739917545846 1103 16 0.9022240641508503 1103 17 0.9103165463817007 1103 18 0.9439087171224614 1103 19 0.8476012942619562 1103 20 0.8892380008040917 1103 21 0.9906622686159068 1103 22 1.0529062303450942 1103 23 0.9918634921038034 1103 24 0.9767063235745042 1103 25 1.020140556437326 1104 1 0.12315343537188284 1104 2 0.5301246720150949 1104 3 0.27362894471897903 1104 4 0.28010869696337437 1104 5 0.21641652205236284 1104 6 0.15792418044520673 1104 7 0.2711376588756167 1104 8 0.3242810894226196 1104 9 0.46890557950135303 1104 10 0.3383570819304893 1104 11 0.4533423612239744 1104 12 0.34511860209022416 1104 13 0.3622643207759637 1104 14 0.45268521109153426 1104 15 0.44395156336527464 1104 16 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6 0.04048502660307918 2210 7 0.009026738697968995 2210 8 0.060098329603690104 2210 9 0.031158747753927685 2210 10 0.11615535464377302 2210 11 0.015813304694447083 2210 12 0.0022174395117033896 2210 13 0.025000323263917323 2210 14 0.155526364498904 2210 15 0.12763881117839304 2210 16 0.3077689471682703 2210 17 0.4524512537911747 2210 18 0.07135459473632011 2210 19 0.23018655101799368 2210 20 0.24118176967161276 2210 21 0.3382166275854104 2210 22 0.17650210811667222 2210 23 0.07557220436481771 2210 24 0.1466394178009267 2210 25 0.19144662275006397 2211 1 -0.182911604125227 2211 2 -0.5036489339851471 2211 3 -0.18174450964826505 2211 4 -0.24152565352123645 2211 5 -0.0777238482703666 2211 6 -0.23748811986025942 2211 7 -0.18722237252492135 2211 8 -0.14487077158794648 2211 9 -0.18598107843760658 2211 10 -0.1485751909335704 2211 11 -0.20919727513733477 2211 12 -0.15820558277220442 2211 13 -0.150934442277034 2211 14 -0.26832449478291664 2211 15 0.02170655344941963 2211 16 -0.004916021776182333 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-0.9602880372342497 2215 25 -0.8690917688962259 2216 1 -0.8675131969424932 2216 2 -1.7999900011546082 2216 3 -1.1799448695762784 2216 4 -0.8990739649880695 2216 5 -0.8039857507249764 2216 6 -0.6799953702268482 2216 7 -0.770098530663887 2216 8 -0.9113360363885334 2216 9 -0.8239995898834422 2216 10 -0.9240869415739397 2216 11 -1.009875484939755 2216 12 -1.0440245061870712 2216 13 -0.9456128458920077 2216 14 -1.0992418216585165 2216 15 -1.1839433171354372 2216 16 -1.2123765978554886 2216 17 -1.2010876597859905 2216 18 -1.2033672804337954 2216 19 -1.346275703801311 2216 20 -1.1364635529584959 2216 21 -1.2901214346556031 2216 22 -1.3658157514872187 2216 23 -1.6439313200765753 2216 24 -1.5791897121818579 2216 25 -1.6734844219424332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12953.0 3 3.0 4 185.0 5 1913.0 6 3077.0 7 2123.0 8 1677.0 9 1208.0 10 1169.0 11 1028.0 12 1005.0 13 957.0 14 952.0 15 985.0 16 976.0 17 1034.0 18 1326.0 19 1708.0 20 2259.0 21 2870.0 22 3383.0 23 4094.0 24 5042.0 25 5848.0 26 6681.0 27 7674.0 28 8644.0 29 10688.0 30 13544.0 31 17949.0 32 24065.0 33 32504.0 34 45370.0 35 64102.0 36 97935.0 37 180422.0 38 471039.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.21387754471982 15.708112520007061 14.022454218921398 24.055555716351716 2 11.089547940706526 22.60556509578703 37.97974174919796 28.325145214308485 3 21.221080285672464 29.13668441205248 27.522746708372175 22.11948859390288 4 14.08239996604768 15.98228306203629 31.518363189511824 38.416953782404214 5 16.39760314262342 33.35241106932637 30.61437054551726 19.635615242532943 6 37.46066068844036 30.59360202589023 13.741633470358444 18.204103815310965 7 24.857170383929862 31.94190471602678 23.117876583650464 20.08304831639289 8 26.486230304408092 33.67099728064286 23.245638962232434 16.597133452716612 9 28.34154803747716 18.692537606713294 15.85131273123421 37.114601624575336 10 14.636060937916874 25.77070553255182 30.37235236270717 29.220881166824146 11 31.561512995350512 25.00493652828655 17.804540450153585 25.629010026209354 12 26.537195631171294 24.150888146773227 26.447133570223848 22.864782651831632 13 32.994372374842406 18.690110770694744 26.140285147134033 22.17523170732882 14 29.779014464612843 19.882437698529653 23.443209683912862 26.895338152944642 15 34.42693205544042 19.45046880898634 24.9497291925083 21.17286994306494 16 26.04017396261884 21.589989830351776 31.47236475138292 20.897471455646468 17 24.843604342098168 28.08833272983613 27.734323839166713 19.33373908889899 18 28.024965523617283 18.249177574557585 35.10292837387037 18.62292852795476 19 24.707913774374223 32.723672755568224 20.5272190078506 22.04119446220695 20 21.181616558695136 26.77382604433205 28.87428723862206 23.170270158350757 21 22.121483536062716 31.845174462839367 21.75734111505976 24.276000886038158 22 20.995737287466316 28.602069139540752 18.97610145756646 31.426092115426474 23 23.548564791898198 23.86000966885147 20.86911803510448 31.722307504145856 24 19.77345305921192 29.416336341889032 21.10515306653107 29.705057532367974 25 26.35327649062655 26.082818853163197 20.594891098617243 26.969013557593012 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 565.0 1 565.0 2 747.0 3 929.0 4 929.0 5 929.0 6 2276.5 7 3624.0 8 3624.0 9 3624.0 10 2993.5 11 2363.0 12 2363.0 13 2363.0 14 2256.0 15 2149.0 16 2149.0 17 2149.0 18 3579.5 19 5010.0 20 5010.0 21 5010.0 22 7698.5 23 10387.0 24 10387.0 25 10387.0 26 15232.0 27 20077.0 28 20077.0 29 20077.0 30 23808.5 31 27540.0 32 27540.0 33 27540.0 34 32418.5 35 37297.0 36 37297.0 37 37297.0 38 41744.5 39 46192.0 40 46192.0 41 46192.0 42 56240.5 43 66289.0 44 66289.0 45 66289.0 46 134757.5 47 203226.0 48 203226.0 49 203226.0 50 285501.5 51 367777.0 52 367777.0 53 367777.0 54 234723.5 55 101670.0 56 101670.0 57 101670.0 58 75075.5 59 48481.0 60 48481.0 61 48481.0 62 42355.5 63 36230.0 64 36230.0 65 36230.0 66 30178.5 67 24127.0 68 24127.0 69 24127.0 70 18789.5 71 13452.0 72 13452.0 73 13452.0 74 11140.5 75 8829.0 76 8829.0 77 8829.0 78 7846.5 79 6864.0 80 6864.0 81 6864.0 82 5046.0 83 3228.0 84 3228.0 85 3228.0 86 2361.0 87 1494.0 88 1494.0 89 1494.0 90 1008.0 91 522.0 92 522.0 93 522.0 94 291.0 95 60.0 96 60.0 97 60.0 98 35.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18133806886031478 2 0.009533971756330943 3 0.0 4 0.1583217128021017 5 0.051329363092165584 6 0.06028551837841586 7 0.04256581329594219 8 0.026868465858750835 9 0.020512484687863545 10 0.0149269254770838 11 0.020512484687863545 12 0.02109030115794421 13 0.011652632146626707 14 0.012904567831801479 15 0.001540843920215102 16 7.70421960107551E-4 17 0.0 18 0.0 19 0.0 20 9.630274501344387E-5 21 0.005970770190833519 22 0.008282036071156173 23 5.778164700806632E-4 24 0.0019260549002688774 25 0.12143776146195272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1038392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.302757377906936 #Duplication Level Percentage of deduplicated Percentage of total 1 88.4792920165559 26.811665189466716 2 6.9566648152164445 4.216122521098513 3 1.485369307277941 1.3503235720509943 4 0.6445040472549601 0.781209990921845 5 0.40371702005662585 0.6116869454053762 6 0.2718027010077955 0.4941822781979406 7 0.19915157958786625 0.42243893983746245 8 0.14986470673059626 0.36330510780547487 9 0.12348360653966081 0.3367704392208238 >10 0.9228591520460553 5.75839853766106 >50 0.15642584704818566 3.370138880885256 >100 0.1644067567116792 10.145338816201578 >500 0.015004111777570718 3.0747027311957185 >1k 0.020431130931160127 13.334481936618431 >5k 0.003830837049592524 7.874814407571616 >10k+ 0.00319236420799377 21.054419705861193 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGAAGCAGTGGTATCAACGCA 46426 4.470951239994145 No Hit CTTCCGTACTCTGCGTTGATACCAC 32895 3.1678787972172358 No Hit TCCGTACTCTGCGTTGATACCACTG 24779 2.3862857186881254 No Hit GAGTACGGAAGCAGTGGTATCAACG 24746 2.383107728102682 No Hit CCCATGTACTCTGCGTTGATACCAC 21159 2.0376697817394587 No Hit GTACATGGGAAGCAGTGGTATCAAC 17099 1.6466806369848768 No Hit GCTTCCGTACTCTGCGTTGATACCA 16733 1.6114338323099564 No Hit GCGTTGATACCACTGCTTCCGTACT 12769 1.2296897510766647 No Hit GTATCAACGCAGAGTACGGAAGCAG 11018 1.0610636445581245 No Hit TATCAACGCAGAGTACGGAAGCAGT 10021 0.965049807779721 No Hit ACTCTGCGTTGATACCACTGCTTCC 9547 0.9194023066433487 No Hit TCCATGTACTCTGCGTTGATACCAC 9339 0.8993713356805523 No Hit GTACATGGAAGCAGTGGTATCAACG 7610 0.7328638895523077 No Hit CATGTACTCTGCGTTGATACCACTG 7190 0.6924167366466614 No Hit CAGTGGTATCAACGCAGAGTACGGA 6551 0.6308792825830707 No Hit ACGCAGAGTACGGAAGCAGTGGTAT 6378 0.6142189076957449 No Hit GGTATCAACGCAGAGTACGGAAGCA 6372 0.6136410912256642 No Hit ATACCACTGCTTCCGTACTCTGCGT 6125 0.5898543132073436 No Hit GAGTACATGGGAAGCAGTGGTATCA 6104 0.5878319555620614 No Hit GCGTTGATACCACTGCTTCCCATGT 5694 0.5483478301065494 No Hit AAGCAGTGGTATCAACGCAGAGTAC 5311 0.5114638787664004 No Hit GTATCAACGCAGAGTACATGGGAAG 5183 0.4991371274046795 No Hit TATCAACGCAGAGTACATGGGAAGC 4934 0.47515774389633203 No Hit GAAGCAGTGGTATCAACGCAGAGTA 4660 0.4487707917626484 No Hit GTGGTATCAACGCAGAGTACGGAAG 4595 0.44251111333677456 No Hit GTACTCTGCGTTGATACCACTGCTT 4218 0.4062049784667062 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3915 0.37702524672763277 No Hit ATCAACGCAGAGTACGGAAGCAGTG 3784 0.36440958713087157 No Hit GCTTCCCATGTACTCTGCGTTGATA 3483 0.335422460881825 No Hit GATACCACTGCTTCCGTACTCTGCG 3412 0.32858496598587045 No Hit GTTGATACCACTGCTTCCGTACTCT 3291 0.3169323338392438 No Hit AAAAAGTACTCTGCGTTGATACCAC 3211 0.30922811423816826 No Hit CCACTGCTTCCGTACTCTGCGTTGA 3166 0.3048944907125633 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3094 0.29796069307159534 No Hit CAGTGGTATCAACGCAGAGTACATG 3031 0.2918936201357484 No Hit CGGAAGCAGTGGTATCAACGCAGAG 2782 0.2679142366274008 No Hit GTACGGGAAGCAGTGGTATCAACGC 2769 0.26666230094222604 No Hit TTCCCGTACTCTGCGTTGATACCAC 2705 0.26049892526136564 No Hit GTATCAACGCAGAGTACTTTTTTTT 2690 0.259054384086164 No Hit GGTATCAACGCAGAGTACATGGGAA 2671 0.25722463193090855 No Hit GAGTACATGGAAGCAGTGGTATCAA 2657 0.25587639350072033 No Hit CCCGTACTCTGCGTTGATACCACTG 2651 0.2552985770306397 No Hit CTGCTTCCGTACTCTGCGTTGATAC 2470 0.23786778018320634 No Hit GCAGAGTACGGAAGCAGTGGTATCA 2438 0.23478609234277614 No Hit CATGGAAGCAGTGGTATCAACGCAG 2243 0.2160070570651546 No Hit ATACCACTGCTTCCCATGTACTCTG 2203 0.2121549472646168 No Hit TATCAACGCAGAGTACTTTTTTTTT 2115 0.20368030570343376 No Hit CTGCTTCCCATGTACTCTGCGTTGA 2104 0.2026209755082859 No Hit GGGAAGCAGTGGTATCAACGCAGAG 2021 0.19462784767217003 No Hit CACTGCTTCCGTACTCTGCGTTGAT 1985 0.19116094885168608 No Hit GTATCAACGCAGAGTACGGGAAGCA 1955 0.18827186650128275 No Hit GCAGTGGTATCAACGCAGAGTACGG 1904 0.1833604265055971 No Hit TACCACTGCTTCCCATGTACTCTGC 1844 0.17758226180479048 No Hit GCGTTGATACCACTGCTTCCATGTA 1842 0.1773896563147636 No Hit GTGGTATCAACGCAGAGTACATGGG 1814 0.17469317945438717 No Hit AAAGTACTCTGCGTTGATACCACTG 1799 0.17324863827918552 No Hit GCTTCCATGTACTCTGCGTTGATAC 1781 0.1715151888689435 No Hit GAGTACGGGAAGCAGTGGTATCAAC 1780 0.17141888612393008 No Hit CCATGTACTCTGCGTTGATACCACT 1766 0.17007064769374186 No Hit CTTCCCGTACTCTGCGTTGATACCA 1716 0.1652555104430697 No Hit TGATACCACTGCTTCCGTACTCTGC 1684 0.16217382260263946 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1588 0.15292875908134884 No Hit TACCACTGCTTCCGTACTCTGCGTT 1570 0.15119530967110686 No Hit CATGGGAAGCAGTGGTATCAACGCA 1562 0.1504248877109993 No Hit TATCAACGCAGAGTACGGGAAGCAG 1532 0.147535805360596 No Hit GTATCAACGCAGAGTACATGGAAGC 1512 0.14560975046032712 No Hit GCGTTGATACCACTGCTTCCCGTAC 1503 0.14474302575520612 No Hit CTGCGTTGATACCACTGCTTCCGTA 1503 0.14474302575520612 No Hit GCAGTGGTATCAACGCAGAGTACAT 1480 0.14252806261989692 No Hit TCAACGCAGAGTACGGAAGCAGTGG 1399 0.13472754027380796 No Hit TATCAACGCAGAGTACATGGAAGCA 1371 0.13203106341343152 No Hit CCACTGCTTCCCATGTACTCTGCGT 1339 0.12894937557300135 No Hit GATACCACTGCTTCCCATGTACTCT 1336 0.12866046733796102 No Hit TACGGAAGCAGTGGTATCAACGCAG 1319 0.12702332067273245 No Hit GTTGATACCACTGCTTCCCATGTAC 1291 0.12432684381235602 No Hit ATCAACGCAGAGTACATGGGAAGCA 1222 0.1176819544064284 No Hit CTTCCCATGTACTCTGCGTTGATAC 1207 0.11623741323122673 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1197 0.11527438578109231 No Hit GCTTCCCGTACTCTGCGTTGATACC 1178 0.11344463362583687 No Hit ACGCAGAGTACATGGAAGCAGTGGT 1143 0.11007403755036634 No Hit GGTATCAACGCAGAGTACTTTTTTT 1112 0.10708865245494957 No Hit TCCCATGTACTCTGCGTTGATACCA 1108 0.1067034414748958 No Hit CTCTGCGTTGATACCACTGCTTCCG 1056 0.10169569873419672 No Hit ACATGGGAAGCAGTGGTATCAACGC 1054 0.10150309324416984 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1051 0.10121418500912949 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.815137250672193E-4 0.0 10 0.0 0.0 0.0 9.630274501344386E-4 0.0 11 0.0 0.0 0.0 0.0013482384301882141 0.0 12 0.0 0.0 0.0 0.002118660390295765 0.0 13 0.0 0.0 0.0 0.003081687840430204 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 25 0.005902347 19.07092 1 TGTCCAA 25 0.0060574017 18.985504 10 CGCATCC 70 4.470312E-7 14.917182 11 TAGGACT 45 6.6683895E-4 14.804291 4 GTTATCA 60 2.4780102E-5 14.30319 1 GGACGGA 130 0.0 13.936442 1 CGAACCC 75 9.747328E-7 13.922703 10 ACGCATC 75 9.747328E-7 13.922703 10 CGTCGTA 150 0.0 13.922703 10 CAGAACC 55 1.9295952E-4 13.842973 4 GTCGTAG 155 0.0 13.473584 11 ATCGAAC 85 2.719662E-7 13.402177 8 TAAACTG 50 0.0014988752 13.300102 5 CCGTCGT 165 0.0 13.232321 9 AATCGAA 80 2.0093285E-6 13.055677 7 CCGACCA 125 1.03682396E-10 12.910142 9 AAAAAGT 510 0.0 12.900917 1 GTATTAG 90 5.160582E-7 12.713946 1 ACCGTCG 165 0.0 12.657612 8 GACCATA 135 2.7284841E-11 12.6570015 11 >>END_MODULE