FastQCFastQC Report
Thu 2 Feb 2017
SRR4050251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9655968
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA158140.16377436213541718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.015.6956671
GTATCAA155450.013.2009161
CGCTTAC5050.012.238296518
ACGCTTA5150.012.18528617
CTCGACG5250.012.1351813
TCGACGC5750.011.07954114
ACCGTCC5350.011.0217398
CGTCGTA14000.010.86942210
GTATTAG14450.010.5461411
CGACGCT6050.010.53014415
AGGCCCG18550.010.51052610
ATACCGT15650.010.4522126
TACCGTC15050.010.3633277
GCGTTAT7400.010.3606941
CGGTCCA15000.010.33500910
CGCCAGT10500.010.32320518
GACGCTT5900.010.31437216
CGCGCTA7350.010.22008716
TAGATCG3350.010.2199685
AACCGCG9000.010.1442657
Produced by FastQC (version 0.11.5)