##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050251_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9655968
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.414521361296973	31.0	31.0	34.0	27.0	34.0
2	30.53997693447203	31.0	31.0	34.0	27.0	34.0
3	30.66657894889461	31.0	31.0	34.0	27.0	34.0
4	34.16892982661086	37.0	35.0	37.0	30.0	37.0
5	34.174629099847884	37.0	35.0	37.0	30.0	37.0
6	34.21179709791913	37.0	35.0	37.0	30.0	37.0
7	34.14680433903675	37.0	35.0	37.0	30.0	37.0
8	34.11900515826068	37.0	35.0	37.0	30.0	37.0
9	35.69151078379713	39.0	37.0	39.0	30.0	39.0
10	35.57119172308773	39.0	37.0	39.0	30.0	39.0
11	35.459390606928274	39.0	37.0	39.0	30.0	39.0
12	35.38067472883092	39.0	37.0	39.0	30.0	39.0
13	35.39109419169575	39.0	37.0	39.0	30.0	39.0
14	36.54904976901332	40.0	37.0	41.0	30.0	41.0
15	36.511003039778096	40.0	37.0	41.0	30.0	41.0
16	36.5121726791141	40.0	37.0	41.0	30.0	41.0
17	36.48357243934528	40.0	37.0	41.0	30.0	41.0
18	36.44872342161863	40.0	37.0	41.0	30.0	41.0
19	36.47024503395206	40.0	37.0	41.0	30.0	41.0
20	36.42608705828354	40.0	37.0	41.0	30.0	41.0
21	36.375207747167345	40.0	37.0	41.0	30.0	41.0
22	36.295133848828	40.0	37.0	41.0	29.0	41.0
23	36.223066087211556	40.0	37.0	41.0	29.0	41.0
24	36.15761278413516	40.0	37.0	41.0	29.0	41.0
25	35.92361024808699	40.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5430052873908551
1101	2	0.5350840168841131
1101	3	0.5408270195784581
1101	4	0.570796480101599
1101	5	0.5287798270619959
1101	6	0.5331859121665445
1101	7	0.5345798778613613
1101	8	0.5195246992963476
1101	9	0.6231303213272099
1101	10	0.6339384666186874
1101	11	0.46819453748456397
1101	12	0.5171816383128416
1101	13	0.4360979545233761
1101	14	0.5942861580808909
1101	15	0.6641815638461708
1101	16	0.6739657710372668
1101	17	0.6844737173707927
1101	18	0.6769305618247969
1101	19	0.6980185364806673
1101	20	0.6737886518353662
1101	21	0.6748193988685571
1101	22	0.7280975700614931
1101	23	0.7040839687901652
1101	24	0.748101425045526
1101	25	0.8113753199069009
1102	1	0.5435528756578272
1102	2	0.5435059559789899
1102	3	0.5642164208123646
1102	4	0.48327963140913255
1102	5	0.4602939727934654
1102	6	0.5006617411621619
1102	7	0.533839564828817
1102	8	0.5624707731348693
1102	9	0.6483559649987285
1102	10	0.6558394518760338
1102	11	0.5466327898115253
1102	12	0.5122953920186788
1102	13	0.5112963821597063
1102	14	0.6737798998780562
1102	15	0.6186465710619515
1102	16	0.6585778892714274
1102	17	0.6482749104308141
1102	18	0.6708414528175553
1102	19	0.7384230147015458
1102	20	0.687699098275651
1102	21	0.7179288764712553
1102	22	0.7197854425982655
1102	23	0.7037421960275694
1102	24	0.655701254476682
1102	25	0.7688451331036958
1103	1	0.5481312952729347
1103	2	0.5440951125244347
1103	3	0.524457292973814
1103	4	0.5180905545483014
1103	5	0.5242804841990605
1103	6	0.5244499284351463
1103	7	0.5286205309784506
1103	8	0.5309907266588283
1103	9	0.549535967654883
1103	10	0.6091909932219934
1103	11	0.5232730236981311
1103	12	0.5265387596589548
1103	13	0.5304872442170137
1103	14	0.7001545727930321
1103	15	0.6273978692918618
1103	16	0.6596563245731986
1103	17	0.5260282293082383
1103	18	0.6225422828565428
1103	19	0.6278178613884577
1103	20	0.6441728443960741
1103	21	0.5889476305247783
1103	22	0.6682086067012349
1103	23	0.6930831247274867
1103	24	0.7479691086629074
1103	25	0.7452729018916173
1104	1	0.4821149963535767
1104	2	0.48056099630236204
1104	3	0.5048314652173111
1104	4	0.5034514634181946
1104	5	0.5276947309891753
1104	6	0.4840754428762466
1104	7	0.4996310528127381
1104	8	0.48312708231013346
1104	9	0.6057809073575697
1104	10	0.5446798981298144
1104	11	0.47038007633432244
1104	12	0.48867359373171126
1104	13	0.49501468744131927
1104	14	0.6106519464232179
1104	15	0.5376842178098755
1104	16	0.6124774841113592
1104	17	0.5534271247070066
1104	18	0.5690979614308986
1104	19	0.6562278143691884
1104	20	0.6538270746566823
1104	21	0.6273539350844999
1104	22	0.6413034439949783
1104	23	0.6150100512846564
1104	24	0.668372984624547
1104	25	0.7357115486750985
1105	1	0.2708738503921708
1105	2	0.35747382536562355
1105	3	0.3477007849657596
1105	4	0.3890264404286512
1105	5	0.3802437004132173
1105	6	0.3220422010762576
1105	7	0.3109295361160491
1105	8	0.3490862659616667
1105	9	0.41009157486523407
1105	10	0.39434529178285516
1105	11	0.335654904874616
1105	12	0.28320243047793525
1105	13	0.3305938516977349
1105	14	0.43681471142055983
1105	15	0.4598832012275551
1105	16	0.3444395281423027
1105	17	0.39922688321529876
1105	18	0.49650495557698804
1105	19	0.4724955979521255
1105	20	0.45206250222017985
1105	21	0.442998391970292
1105	22	0.44619115104124063
1105	23	0.4521238992489103
1105	24	0.4157038720483257
1105	25	0.4476518389891595
1106	1	0.29275665304676934
1106	2	0.258614412150024
1106	3	0.28812326363803464
1106	4	0.34032949547690095
1106	5	0.335918034455311
1106	6	0.31432090917699895
1106	7	0.3135797735111936
1106	8	0.303384322280948
1106	9	0.25381785580876937
1106	10	0.3392926532140166
1106	11	0.3104573934057555
1106	12	0.30043001990632945
1106	13	0.25040163152573314
1106	14	0.303587610055736
1106	15	0.3794495205915567
1106	16	0.2381029674281976
1106	17	0.3508427201238362
1106	18	0.4197712788298773
1106	19	0.35338457749008967
1106	20	0.39859246666608783
1106	21	0.35914398701821426
1106	22	0.4009723065920028
1106	23	0.2401553112188637
1106	24	0.37954430889262625
1106	25	0.4014084251447301
1107	1	0.36222411246559005
1107	2	0.3337816669347937
1107	3	0.3290613770683706
1107	4	0.36327636100079985
1107	5	0.37074804736597144
1107	6	0.3139626781858311
1107	7	0.3952009481836285
1107	8	0.3341103968102104
1107	9	0.41691441111841954
1107	10	0.44967897632081844
1107	11	0.3876727361622727
1107	12	0.38665537333822186
1107	13	0.37307613756796343
1107	14	0.4259583371371747
1107	15	0.41485276746849564
1107	16	0.38829162777930293
1107	17	0.4249409704041085
1107	18	0.425156516115095
1107	19	0.471648607029735
1107	20	0.48212477378550744
1107	21	0.424077129618766
1107	22	0.4855705233576799
1107	23	0.4333673894471701
1107	24	0.49196595425575396
1107	25	0.546256615588014
1108	1	0.16705225685248237
1108	2	0.11896470754511412
1108	3	0.16612669543933478
1108	4	0.1958428586965013
1108	5	0.19734415396797544
1108	6	0.1953164487015897
1108	7	0.1596601430439364
1108	8	0.16232384311497583
1108	9	0.11109767425352146
1108	10	0.07482282158562015
1108	11	0.12156857874637694
1108	12	0.10850945407390356
1108	13	0.13239939783758103
1108	14	-0.03501049881956675
1108	15	0.11874982691796276
1108	16	0.13366086749410755
1108	17	0.08143397538081132
1108	18	0.014049091903380884
1108	19	0.17401908301660995
1108	20	0.19595758863796675
1108	21	0.26412065684804276
1108	22	0.2025839945234651
1108	23	0.16551331175889317
1108	24	0.1434899843722306
1108	25	0.11493987429341246
1109	1	0.02488290111220337
1109	2	0.034664370338884254
1109	3	0.12393844090676964
1109	4	0.10185139471730764
1109	5	0.1265338828015814
1109	6	0.09028733255765076
1109	7	0.037242691753796464
1109	8	0.04853230572443579
1109	9	0.0295837519142097
1109	10	-0.08761395224627222
1109	11	-0.02100653880071235
1109	12	-0.01762934549736883
1109	13	-0.03867436269884905
1109	14	-0.010926045334173295
1109	15	0.048367472207260676
1109	16	0.06969963688227665
1109	17	-0.03502044956874073
1109	18	0.08224516873537624
1109	19	0.04389314265073807
1109	20	0.01925200969424168
1109	21	0.017177338545515397
1109	22	0.03639374771760373
1109	23	0.05754829336194689
1109	24	0.04052346513567073
1109	25	0.014216425126065246
1110	1	0.009259433863181954
1110	2	-0.00897071262758331
1110	3	0.06011298534076914
1110	4	0.03218063735248222
1110	5	0.022654307829590437
1110	6	0.043383620946436
1110	7	0.06686072816753352
1110	8	0.014648116117605525
1110	9	0.056738922766513156
1110	10	-0.019879175906361013
1110	11	0.01161563181867109
1110	12	-0.01293663247921728
1110	13	-0.018871419824066038
1110	14	-0.051294861490852384
1110	15	-0.0245629326140957
1110	16	0.005895584335746662
1110	17	0.07303459656446165
1110	18	0.025621489596019842
1110	19	0.0022309066443995107
1110	20	-0.026451623383294987
1110	21	-0.004600497917053303
1110	22	0.013157549197877927
1110	23	0.09549142953924417
1110	24	0.06158570999977542
1110	25	0.0951834440009307
1111	1	-0.13393342935719232
1111	2	-0.15848917510692218
1111	3	-0.1563320159592223
1111	4	-0.06543228407759472
1111	5	-0.16300424806403413
1111	6	-0.12568160914312188
1111	7	-0.12513119350593627
1111	8	-0.10355519645575839
1111	9	-0.09012736606505456
1111	10	-0.09123151283363029
1111	11	-0.19480995044717986
1111	12	-0.18420140112468175
1111	13	-0.15338414871597905
1111	14	-0.15967387206358552
1111	15	-0.16753500288240275
1111	16	-0.21578765559935675
1111	17	-0.1831848656629873
1111	18	-0.17869231449894585
1111	19	-0.15774474472722488
1111	20	-0.18022057134716363
1111	21	-0.11353382386170807
1111	22	-0.15897532221060118
1111	23	-0.16758310399238496
1111	24	-0.19945538705065502
1111	25	-0.24803553571785386
1112	1	-0.06574403706150633
1112	2	-0.05264210199726804
1112	3	-0.05669256565358083
1112	4	-0.1157070903356896
1112	5	0.0024199174589938366
1112	6	-0.12070588288892736
1112	7	-0.1240961526717399
1112	8	-0.061009525103678186
1112	9	-0.07214208226464791
1112	10	-0.142076616072913
1112	11	-0.05680176648534285
1112	12	-0.14611957609572102
1112	13	-0.10950263743783495
1112	14	-0.12179829861806013
1112	15	-0.15474144862733397
1112	16	-0.1056228380985047
1112	17	-0.06472913525156088
1112	18	-0.11019302377682294
1112	19	-0.23056786724453815
1112	20	-0.14519759268914356
1112	21	-0.16009052679289226
1112	22	-0.1238509588346588
1112	23	-0.10636138734429323
1112	24	-0.0883270948972168
1112	25	-0.06076671462755456
1113	1	-0.30062479092365635
1113	2	-0.27100187458115954
1113	3	-0.2312709236282906
1113	4	-0.15458666878343053
1113	5	-0.14756950099295096
1113	6	-0.23662608521749462
1113	7	-0.2926606718109852
1113	8	-0.2273104075975354
1113	9	-0.2287526372789941
1113	10	-0.3153457279158616
1113	11	-0.2468993502794561
1113	12	-0.44841629774527547
1113	13	-0.3617794129464329
1113	14	-0.3401142583149408
1113	15	-0.2726038596859013
1113	16	-0.3036912613624594
1113	17	-0.2823845288555944
1113	18	-0.2217003691121846
1113	19	-0.354970905715156
1113	20	-0.2762955688189237
1113	21	-0.2993852899826379
1113	22	-0.31838275366207114
1113	23	-0.28537295994160417
1113	24	-0.3516697282582655
1113	25	-0.29624877941431293
1114	1	-0.31807302837282947
1114	2	-0.3003803362009272
1114	3	-0.341268492193759
1114	4	-0.2876508844719865
1114	5	-0.2898619429721023
1114	6	-0.2800567784038108
1114	7	-0.38010963386093977
1114	8	-0.34575452267180395
1114	9	-0.38306441292428417
1114	10	-0.4263737259838507
1114	11	-0.43684937984415484
1114	12	-0.4975404593154593
1114	13	-0.3700467985120497
1114	14	-0.5961387660578907
1114	15	-0.6032850208251404
1114	16	-0.5619085757699978
1114	17	-0.5702854767085341
1114	18	-0.5743157030604564
1114	19	-0.43275096901210475
1114	20	-0.5683171729201533
1114	21	-0.5614802246008921
1114	22	-0.6264482561687501
1114	23	-0.5212883849208794
1114	24	-0.51904835028256
1114	25	-0.5458295208696811
1115	1	-0.45610709567182894
1115	2	-0.47999380718582785
1115	3	-0.4891904991236977
1115	4	-0.35105124489241035
1115	5	-0.43971381157325595
1115	6	-0.42328826465746516
1115	7	-0.49113144719854773
1115	8	-0.5125326482914829
1115	9	-0.42811795918473194
1115	10	-0.4940203193836723
1115	11	-0.5727121395635564
1115	12	-0.6179009303494638
1115	13	-0.5615364053797336
1115	14	-0.6603835645751346
1115	15	-0.6111757357567811
1115	16	-0.659315662515894
1115	17	-0.6521289173600877
1115	18	-0.5935999863615606
1115	19	-0.6347883929237099
1115	20	-0.6387601347364864
1115	21	-0.7089589727966867
1115	22	-0.8695833330171325
1115	23	-0.7687242217667531
1115	24	-0.673066553105997
1115	25	-0.6628620421141207
1116	1	-0.2824954907622441
1116	2	-0.43832392735161463
1116	3	-0.387314570539143
1116	4	-0.4256384300624987
1116	5	-0.4091007542284757
1116	6	-0.37155418072818946
1116	7	-0.42437729682932
1116	8	-0.43271509403110286
1116	9	-0.47272159660723645
1116	10	-0.48853556757457284
1116	11	-0.518275677406244
1116	12	-0.59955223098126
1116	13	-0.5595347063235039
1116	14	-0.559477783076872
1116	15	-0.6868146282204961
1116	16	-0.605446178377008
1116	17	-0.641226796369061
1116	18	-0.5843303214006283
1116	19	-0.594668845564712
1116	20	-0.6105508501177823
1116	21	-0.6678946915856017
1116	22	-0.7198788213293028
1116	23	-0.6155308986621364
1116	24	-0.6865219530051405
1116	25	-0.6759762724736973
1201	1	0.5292988701317292
1201	2	0.5301652505079737
1201	3	0.562543125844563
1201	4	0.5822814861560701
1201	5	0.6198333300867986
1201	6	0.60360776928961
1201	7	0.5914228772819854
1201	8	0.6222070416852574
1201	9	0.7055676964478579
1201	10	0.712944482946078
1201	11	0.6197108982909754
1201	12	0.6227992716812594
1201	13	0.6832090894757883
1201	14	0.743379238728167
1201	15	0.8046599481007419
1201	16	0.7321902048499354
1201	17	0.7405802739154694
1201	18	0.7494240744164387
1201	19	0.7382615843866418
1201	20	0.7241506459401208
1201	21	0.739445345184194
1201	22	0.7588786218317196
1201	23	0.8100595575405904
1201	24	0.7945820386337132
1201	25	0.7060164852047635
1202	1	0.41963920260408116
1202	2	0.39193496086739543
1202	3	0.46571946567692635
1202	4	0.5540296577598767
1202	5	0.5339491712996391
1202	6	0.4846387748303229
1202	7	0.5051072763516871
1202	8	0.5024660817086826
1202	9	0.4991081342865442
1202	10	0.5777200985547424
1202	11	0.4429988892771348
1202	12	0.4284268212202207
1202	13	0.39793785025490536
1202	14	0.5354588379416185
1202	15	0.5415654918376376
1202	16	0.5379459941514071
1202	17	0.5753335646541728
1202	18	0.6053710754955759
1202	19	0.6156906843714438
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2210	15	-0.04218268620605414
2210	16	0.04317237110647909
2210	17	-0.044298345010162166
2210	18	-0.04774196199260672
2210	19	-0.0032156564942127375
2210	20	-0.06776652993504229
2210	21	-0.0782228866388337
2210	22	-0.10528009790820647
2210	23	-0.12145593485397654
2210	24	-0.05625709832131065
2210	25	-0.09634023037173733
2211	1	-0.22296027173777588
2211	2	-0.26517388322064406
2211	3	-0.2324315153397869
2211	4	-0.3289241335481776
2211	5	-0.3353612582393808
2211	6	-0.23172341636629312
2211	7	-0.22294763031570852
2211	8	-0.28568285636875146
2211	9	-0.2222844666504642
2211	10	-0.31937184054014267
2211	11	-0.123733736573719
2211	12	-0.18609702591057697
2211	13	-0.12229672483717025
2211	14	-0.1465296122694255
2211	15	-0.07268770937302804
2211	16	-0.12664907115286184
2211	17	-0.3123762589519856
2211	18	-0.2584865083537764
2211	19	-0.27902363015387976
2211	20	-0.2581177059267503
2211	21	-0.29505229126817767
2211	22	-0.3607554016296035
2211	23	-0.3406772724339078
2211	24	-0.37245859871151765
2211	25	-0.37809912392269496
2212	1	-0.28805980637900674
2212	2	-0.24810203015660193
2212	3	-0.3330002880915188
2212	4	-0.245837420634885
2212	5	-0.21327340124518201
2212	6	-0.2708661387661664
2212	7	-0.27426238464031627
2212	8	-0.25593020329457516
2212	9	-0.2757410860216041
2212	10	-0.3377687533274667
2212	11	-0.13454443595914256
2212	12	-0.1806136001827099
2212	13	-0.21749877372267434
2212	14	-0.13262531724943472
2212	15	-0.26096179828748234
2212	16	-0.18737223005712877
2212	17	-0.19947436833998466
2212	18	-0.3216361430035448
2212	19	-0.3387261086357114
2212	20	-0.26289292936301223
2212	21	-0.2313855452882123
2212	22	-0.23573213924217384
2212	23	-0.28332701730440135
2212	24	-0.35511370529744113
2212	25	-0.3744381545005382
2213	1	-0.3911496958887746
2213	2	-0.4062598415313765
2213	3	-0.43158154130485116
2213	4	-0.418638646454184
2213	5	-0.41578545140477274
2213	6	-0.3286654124475703
2213	7	-0.36391491062673254
2213	8	-0.4324645095817701
2213	9	-0.32382360403167354
2213	10	-0.2858546599696723
2213	11	-0.25431779036078694
2213	12	-0.3747282769649729
2213	13	-0.32036280885927937
2213	14	-0.5300521655614858
2213	15	-0.4326912079277534
2213	16	-0.3735844043998853
2213	17	-0.48459815486775426
2213	18	-0.4075805133403705
2213	19	-0.3896697181633826
2213	20	-0.42652112390919683
2213	21	-0.3746331670502414
2213	22	-0.39750907506054034
2213	23	-0.4131145594597001
2213	24	-0.4331897208082367
2213	25	-0.39301766113937475
2214	1	-0.4261413604802762
2214	2	-0.49461005149179016
2214	3	-0.5373355292314876
2214	4	-0.6948374841272198
2214	5	-0.5316836674189318
2214	6	-0.5313670261069987
2214	7	-0.5540948906352483
2214	8	-0.5228010337543694
2214	9	-0.5367815028397018
2214	10	-0.5086509591149593
2214	11	-0.46057130444235383
2214	12	-0.44227499555120176
2214	13	-0.5269857409850474
2214	14	-0.6694958334159082
2214	15	-0.5232170171338169
2214	16	-0.6072431545869676
2214	17	-0.6294816300212958
2214	18	-0.6903425921932183
2214	19	-0.6620962237865768
2214	20	-0.6610499507528047
2214	21	-0.6580959519099565
2214	22	-0.6039300322046941
2214	23	-0.5715282500825367
2214	24	-0.6636617358784278
2214	25	-0.6279506427523245
2215	1	-0.598601309461813
2215	2	-0.5808972890682007
2215	3	-0.7201614854445175
2215	4	-0.5882634444989918
2215	5	-0.6025743920032767
2215	6	-0.6189310414770617
2215	7	-0.577359075415508
2215	8	-0.630779022823198
2215	9	-0.815847109056044
2215	10	-0.7356524155070829
2215	11	-0.6888301473318137
2215	12	-0.6367653531879682
2215	13	-0.6346489760164431
2215	14	-0.7325592788698998
2215	15	-0.7234927582335118
2215	16	-0.7763225229042519
2215	17	-0.7234892047082297
2215	18	-0.7726220836558113
2215	19	-0.8849638788865093
2215	20	-0.8437668965410552
2215	21	-0.7611773996950078
2215	22	-0.9183733505523861
2215	23	-0.8169781744611839
2215	24	-0.929737751232949
2215	25	-0.8548230902211458
2216	1	-0.4969203335667771
2216	2	-0.621089484806074
2216	3	-0.622818682838318
2216	4	-0.5036981703925036
2216	5	-0.5454966923256777
2216	6	-0.5136411987292604
2216	7	-0.5858478536651717
2216	8	-0.5570114475074703
2216	9	-0.561531130010664
2216	10	-0.6262571536833335
2216	11	-0.46674608863994393
2216	12	-0.5001287956431852
2216	13	-0.5068890761095872
2216	14	-0.5644479068897823
2216	15	-0.499884582771692
2216	16	-0.7132484295918644
2216	17	-0.7946647050277633
2216	18	-0.6821806197494666
2216	19	-0.6957747535060648
2216	20	-0.5904106719446318
2216	21	-0.6448071024003426
2216	22	-0.6261792090383835
2216	23	-0.7683284006235738
2216	24	-0.9038229270324649
2216	25	-0.8220187788435567
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	284434.0
3	11.0
4	2435.0
5	13255.0
6	16978.0
7	16948.0
8	14060.0
9	13637.0
10	13860.0
11	16409.0
12	26710.0
13	23209.0
14	10850.0
15	10830.0
16	11556.0
17	12302.0
18	13816.0
19	16388.0
20	20871.0
21	25829.0
22	32317.0
23	39151.0
24	45979.0
25	53512.0
26	60342.0
27	68708.0
28	80066.0
29	97115.0
30	122880.0
31	157715.0
32	204457.0
33	271599.0
34	372758.0
35	526326.0
36	786912.0
37	1460178.0
38	4711565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.325382971359716	17.94762842088636	12.927170335252075	25.799818272501845
2	16.119081662968266	20.392495680591125	39.07487265834928	24.413549998091327
3	18.734752572409295	24.353804746402634	28.433587120384118	28.477855560803956
4	12.837635422281968	16.683381783825542	37.430067598943026	33.04891519494946
5	15.278871829521053	35.624160093364	33.46156910953088	15.635398967584067
6	32.308248592161036	36.881069553431765	16.81050608828558	14.00017576612162
7	28.903181817239798	30.472599233611664	21.685795288514743	18.938423660633795
8	25.957982886752717	34.0486823512539	20.098086355453663	19.895248406539725
9	27.139297942605783	15.092079446276617	19.14231490556083	38.62630770555677
10	16.17498327592827	26.725133986889155	31.35987041500052	25.740012322182054
11	36.04172547173924	21.853915578133922	23.10478683200306	18.99957211812377
12	25.02817273829841	23.711175422528516	29.161974685824966	22.098677153348113
13	29.589169925774705	20.698743727237943	24.71798245659765	24.994103890389706
14	23.073374097010184	20.98311395357445	25.3698680697796	30.573643879635764
15	24.381460164193243	28.357231348818996	22.541798501787216	24.719509985200546
16	24.363234820291815	26.63411979016232	24.945241374851804	24.057404014694058
17	24.272079303636733	26.100683488814507	25.249851789463673	24.377385418085083
18	25.420597916296057	24.65284287716065	26.719873268489657	23.206685938053635
19	26.744761955208617	24.753918093581298	24.78589763668361	23.71542231452647
20	25.80009849932945	25.284994764811735	25.128069462152574	23.786837273706237
21	25.61783284755134	24.976110577918092	24.65651103396755	24.749545540563016
22	25.052529507444632	25.595164115610793	25.392959919923257	23.95934645702131
23	23.858227444825143	25.07688962067787	25.74231765781007	25.322565276686916
24	24.628902749483668	25.709849811582863	25.661691178149514	23.99955626078395
25	24.465805706093104	25.291792406151757	25.6386259187679	24.603775968987236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3795.0
1	3795.0
2	4463.5
3	5132.0
4	5132.0
5	5132.0
6	8668.5
7	12205.0
8	12205.0
9	12205.0
10	17523.5
11	22842.0
12	22842.0
13	22842.0
14	33664.0
15	44486.0
16	44486.0
17	44486.0
18	71865.0
19	99244.0
20	99244.0
21	99244.0
22	151278.5
23	203313.0
24	203313.0
25	203313.0
26	289943.0
27	376573.0
28	376573.0
29	376573.0
30	471446.0
31	566319.0
32	566319.0
33	566319.0
34	661656.5
35	756994.0
36	756994.0
37	756994.0
38	822294.0
39	887594.0
40	887594.0
41	887594.0
42	961007.5
43	1034421.0
44	1034421.0
45	1034421.0
46	1077816.5
47	1121212.0
48	1121212.0
49	1121212.0
50	1118675.5
51	1116139.0
52	1116139.0
53	1116139.0
54	1022677.0
55	929215.0
56	929215.0
57	929215.0
58	860133.0
59	791051.0
60	791051.0
61	791051.0
62	701853.5
63	612656.0
64	612656.0
65	612656.0
66	521980.0
67	431304.0
68	431304.0
69	431304.0
70	344926.5
71	258549.0
72	258549.0
73	258549.0
74	213822.5
75	169096.0
76	169096.0
77	169096.0
78	140736.5
79	112377.0
80	112377.0
81	112377.0
82	86550.0
83	60723.0
84	60723.0
85	60723.0
86	44542.0
87	28361.0
88	28361.0
89	28361.0
90	19760.5
91	11160.0
92	11160.0
93	11160.0
94	6073.0
95	986.0
96	986.0
97	986.0
98	603.5
99	221.0
100	221.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1455990740648685
2	0.16321512250247724
3	0.1765436670875463
4	0.19659344355739372
5	0.18633036066399558
6	0.07023635538145942
7	0.057042442559875915
8	0.06110210804344008
9	0.06761621413824072
10	0.06941820851104726
11	0.7167587962180487
12	0.6745672727995785
13	0.6721024758988431
14	0.6722578202413264
15	0.6739251828506474
16	0.6803875074979535
17	0.6719367752668608
18	0.6827901666616957
19	0.677943423176216
20	0.6840847028490566
21	0.6721542573463375
22	0.6748986740635429
23	0.6591364014462351
24	0.6705593887635087
25	0.6632789172457904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9655968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.36525303433104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.00049985654708	40.97340677394404
2	11.092413409678285	10.951595894603505
3	2.552920783324287	3.780767413362181
4	0.9727051923635972	1.9207135179534651
5	0.5050159362601527	1.2465119739926018
6	0.32270431729332233	0.9558228167073545
7	0.21343179986559108	0.7375280364156324
8	0.1582884556845187	0.6251159733823808
9	0.1298573857048284	0.576939843332602
>10	0.8419390646471683	8.09746594434937
>50	0.0992058070465793	3.4305566044409175
>100	0.08755953088660787	8.90220789817701
>500	0.011783048697477087	4.016685900828118
>1k	0.010832422008952965	10.749315629995406
>5k	8.219152418620067E-4	2.870843191804202
>10k+	2.1074749791333505E-5	0.16452258671118603
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	15814	0.16377436213541718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.213773699332889E-5
2	0.0	0.0	0.0	0.0	6.213773699332889E-5
3	0.0	0.0	0.0	1.0356289498888148E-5	6.213773699332889E-5
4	0.0	0.0	0.0	2.0712578997776296E-5	6.213773699332889E-5
5	0.0	0.0	0.0	3.1068868496664446E-5	8.285031599110518E-5
6	0.0	0.0	0.0	3.1068868496664446E-5	8.285031599110518E-5
7	0.0	0.0	0.0	4.142515799555259E-5	8.285031599110518E-5
8	0.0	0.0	0.0	5.1781447494440745E-5	8.285031599110518E-5
9	0.0	0.0	0.0	8.285031599110518E-5	8.285031599110518E-5
10	0.0	0.0	0.0	8.285031599110518E-5	9.320660548999334E-5
11	0.0	0.0	0.0	8.285031599110518E-5	9.320660548999334E-5
12	0.0	0.0	0.0	8.285031599110518E-5	1.9676950047887483E-4
13	0.0	0.0	0.0	1.0356289498888149E-4	2.0712578997776298E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4740	0.0	15.695667	1
GTATCAA	15545	0.0	13.200916	1
CGCTTAC	505	0.0	12.2382965	18
ACGCTTA	515	0.0	12.185286	17
CTCGACG	525	0.0	12.13518	13
TCGACGC	575	0.0	11.079541	14
ACCGTCC	535	0.0	11.021739	8
CGTCGTA	1400	0.0	10.869422	10
GTATTAG	1445	0.0	10.546141	1
CGACGCT	605	0.0	10.530144	15
AGGCCCG	1855	0.0	10.510526	10
ATACCGT	1565	0.0	10.452212	6
TACCGTC	1505	0.0	10.363327	7
GCGTTAT	740	0.0	10.360694	1
CGGTCCA	1500	0.0	10.335009	10
CGCCAGT	1050	0.0	10.323205	18
GACGCTT	590	0.0	10.314372	16
CGCGCTA	735	0.0	10.220087	16
TAGATCG	335	0.0	10.219968	5
AACCGCG	900	0.0	10.144265	7
>>END_MODULE