FastQCFastQC Report
Thu 2 Feb 2017
SRR4050251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9655968
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA155680.1612267149186907No Hit
GTATCAACGCAGAGTACTTTTTTTT137290.1421814985302354No Hit
GAATAGGACCGCGGTTCTATTTTGT103420.10710474599750124No Hit
GATTAAGAGGGACGGCCGGGGGCAT97560.10103596035115278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45600.015.8871091
GTATCAA154300.013.6040341
ACGCTTA4850.011.54611217
CTCGACG4800.011.27149613
AACCGCG8050.011.206427
GAGCGTT5000.011.0153227
CGCCAGT11900.011.00671318
CGTCGTA15350.010.94844210
CGCTTAC5200.010.768970518
GCGTTAT6600.010.6736471
CGTTATT6600.010.6722062
CGAGCCG18300.010.63260115
ATCGCCA12500.010.55459316
CGAACGA7750.010.53254816
GGTAGCG4450.010.45498811
CCGTCGT16750.010.2035649
CGACCAT15950.010.17941610
TACCGTC16900.010.1702377
ATACCGT17500.010.1509056
GCATCGC12950.010.11453714
Produced by FastQC (version 0.11.5)