##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9655968
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.403196965855727	31.0	31.0	34.0	28.0	34.0
2	31.49665264010817	33.0	31.0	34.0	28.0	34.0
3	31.589558498951114	33.0	31.0	34.0	28.0	34.0
4	35.10318799730902	37.0	35.0	37.0	32.0	37.0
5	35.037634652476065	37.0	35.0	37.0	32.0	37.0
6	35.059992845875215	37.0	35.0	37.0	32.0	37.0
7	34.99395244474712	37.0	35.0	37.0	32.0	37.0
8	34.9793748280856	37.0	35.0	37.0	32.0	37.0
9	36.645682649321124	39.0	37.0	39.0	33.0	39.0
10	36.534588764171545	39.0	37.0	39.0	32.0	39.0
11	36.56154421804215	39.0	37.0	39.0	32.0	39.0
12	36.45086365240647	39.0	37.0	39.0	32.0	39.0
13	36.4859136857123	39.0	37.0	39.0	32.0	39.0
14	37.71538669142234	40.0	38.0	41.0	33.0	41.0
15	37.7073068179182	40.0	38.0	41.0	33.0	41.0
16	37.68032184862253	40.0	38.0	41.0	32.0	41.0
17	37.656897993033944	40.0	38.0	41.0	32.0	41.0
18	37.61478755936225	40.0	38.0	41.0	32.0	41.0
19	37.632487493744804	40.0	38.0	41.0	32.0	41.0
20	37.59220515229545	40.0	38.0	41.0	32.0	41.0
21	37.55250711269963	40.0	38.0	41.0	32.0	41.0
22	37.51389275523697	40.0	38.0	41.0	32.0	41.0
23	37.44846834620827	40.0	38.0	41.0	32.0	41.0
24	37.39265685221824	40.0	38.0	41.0	32.0	41.0
25	37.09813619929147	40.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4440930666015248
1101	2	0.413509741817208
1101	3	0.391446559957469
1101	4	0.28377157010879017
1101	5	0.39613203316837087
1101	6	0.42888380034807483
1101	7	0.4486598013310399
1101	8	0.4383324616326192
1101	9	0.5038841095954325
1101	10	0.4910433037161752
1101	11	0.48475031176292305
1101	12	0.5609387728560407
1101	13	0.5373306424402386
1101	14	0.5581800279725186
1101	15	0.5893217566872693
1101	16	0.6226168302779413
1101	17	0.5926343514296022
1101	18	0.644403078576957
1101	19	0.6699006476749005
1101	20	0.7114296271505367
1101	21	0.6511938878419201
1101	22	0.6446855692631743
1101	23	0.6506567585328185
1101	24	0.6824811404302551
1101	25	0.7363398446355873
1102	1	0.41689137693336775
1102	2	0.36299796870531864
1102	3	0.4182155689547393
1102	4	0.3542006103660853
1102	5	0.3434256355973062
1102	6	0.3727977284332127
1102	7	0.3934771337969849
1102	8	0.4518904261026293
1102	9	0.4658117034694982
1102	10	0.4925506336592278
1102	11	0.45939736629035366
1102	12	0.5023316069774353
1102	13	0.48905837924714746
1102	14	0.5443907630543805
1102	15	0.6191626181685095
1102	16	0.4928704618056017
1102	17	0.5453251077188668
1102	18	0.5698471777932852
1102	19	0.607264576461688
1102	20	0.6291847918208688
1102	21	0.5398556998726391
1102	22	0.6078125073301521
1102	23	0.62286213931813
1102	24	0.6706522290112247
1102	25	0.8520926537923827
1103	1	0.34818544730886813
1103	2	0.35804986979266573
1103	3	0.3894779226539562
1103	4	0.2428124538740164
1103	5	0.3079027224023321
1103	6	0.34128978448422487
1103	7	0.41149194962197555
1103	8	0.398745634349261
1103	9	0.4420922926858921
1103	10	0.49085934558363675
1103	11	0.49407959231163545
1103	12	0.5599305793832343
1103	13	0.49833693367082077
1103	14	0.5945534872308258
1103	15	0.6257817847781979
1103	16	0.5894394232404139
1103	17	0.5542486418638362
1103	18	0.6083331018407065
1103	19	0.6130841162627974
1103	20	0.5845485781928375
1103	21	0.5329423856933531
1103	22	0.6112669126736066
1103	23	0.6701964133675489
1103	24	0.6411039843063193
1103	25	0.7509085798582191
1104	1	0.3792093389081188
1104	2	0.32515498207897053
1104	3	0.3407190105563238
1104	4	0.2893578054466417
1104	5	0.33018847189910616
1104	6	0.33962691368979137
1104	7	0.3422010813372651
1104	8	0.3428023468792176
1104	9	0.41750974041330124
1104	10	0.4333926038118605
1104	11	0.4089810757379695
1104	12	0.4479943535027999
1104	13	0.4541916598145974
1104	14	0.5146606292830143
1104	15	0.529386500157301
1104	16	0.59178165773438
1104	17	0.5772993850854888
1104	18	0.6821577926955413
1104	19	0.6446367842227261
1104	20	0.6409113114898588
1104	21	0.6525755116703778
1104	22	0.6105712825678253
1104	23	0.5939692570217971
1104	24	0.6283240931921839
1104	25	0.7678794238906264
1105	1	0.30263165459314223
1105	2	0.2750658656227998
1105	3	0.279002364091383
1105	4	0.21802051271699696
1105	5	0.23393772790237222
1105	6	0.33373712948930034
1105	7	0.2816459613546698
1105	8	0.3225153273397794
1105	9	0.35786802163895004
1105	10	0.4149289700958221
1105	11	0.3803269157086291
1105	12	0.4500973049344097
1105	13	0.4064278996443065
1105	14	0.4560827473814726
1105	15	0.48909313036136837
1105	16	0.4645033539814847
1105	17	0.4549481636544215
1105	18	0.430870375314953
1105	19	0.4724694762698576
1105	20	0.5560110655469828
1105	21	0.5493652347426448
1105	22	0.5534376118376017
1105	23	0.5432306135900618
1105	24	0.5374944171330043
1105	25	0.598757557604678
1106	1	0.2551949211761553
1106	2	0.21452048479432406
1106	3	0.2502006727833006
1106	4	0.18515683328069343
1106	5	0.21124066012767884
1106	6	0.2531119321354893
1106	7	0.2988434174931385
1106	8	0.3113207303634766
1106	9	0.3740019068397302
1106	10	0.3404730829049498
1106	11	0.37719042592553365
1106	12	0.47216824592489104
1106	13	0.4086294222284934
1106	14	0.41960024845091937
1106	15	0.38340350163353065
1106	16	0.3745550612430364
1106	17	0.3902280130476541
1106	18	0.4306064298423351
1106	19	0.4441576128039344
1106	20	0.4798518075717837
1106	21	0.42365404780896654
1106	22	0.44944276717335185
1106	23	0.45842771852695563
1106	24	0.5396546859200697
1106	25	0.6253084019524096
1107	1	0.22016354933852966
1107	2	0.18930344647856145
1107	3	0.21058841409854878
1107	4	0.17137479952049262
1107	5	0.24414073691327332
1107	6	0.2521413146920466
1107	7	0.27116103257642976
1107	8	0.3061683708141203
1107	9	0.35956607622834014
1107	10	0.3534995251898252
1107	11	0.3620893939333243
1107	12	0.37828932645477664
1107	13	0.24959624564245075
1107	14	0.3353175048875059
1107	15	0.26704334794903417
1107	16	0.2699131307313536
1107	17	0.3213336358412917
1107	18	0.3207016492033148
1107	19	0.31464052234371565
1107	20	0.2903713880087011
1107	21	0.16509639228300443
1107	22	0.11526884489588696
1107	23	0.30086649422936773
1107	24	0.35931679950609663
1107	25	0.5202433036096821
1108	1	0.17127569375064056
1108	2	0.18054451584600884
1108	3	0.16379458973085903
1108	4	0.18188740494364453
1108	5	0.2007316312585843
1108	6	0.21081167382170207
1108	7	0.18426684587629438
1108	8	0.19089291403773956
1108	9	0.25145037622815636
1108	10	0.24813694543379938
1108	11	0.22164355114347956
1108	12	0.16504426477990108
1108	13	0.16899825827771764
1108	14	0.17292004782863302
1108	15	0.23421403215245107
1108	16	0.2133957017695991
1108	17	0.17792379479372755
1108	18	0.15661621457987707
1108	19	0.22270894461094315
1108	20	0.1831286066539377
1108	21	0.2131327386471753
1108	22	0.21247341310169787
1108	23	0.1379903657491468
1108	24	0.2081823861932719
1108	25	0.3950970997957626
1109	1	0.03146873224111957
1109	2	0.08827577241647688
1109	3	0.03200119341071073
1109	4	0.030788225224270604
1109	5	0.07924020994357761
1109	6	0.09246837297534682
1109	7	0.08093594548677885
1109	8	0.06785845893978149
1109	9	0.07318693950140442
1109	10	0.054059997592730724
1109	11	0.029542317106937332
1109	12	0.027220673128603323
1109	13	0.05889979509988308
1109	14	0.04876496159850774
1109	15	0.05132129521828688
1109	16	-0.011003928035350441
1109	17	0.02347711180475187
1109	18	-0.08577535834487549
1109	19	-0.05233057219607673
1109	20	-0.021624775857475242
1109	21	0.04732354095531832
1109	22	0.09145857434994298
1109	23	0.13226154053162986
1109	24	0.11127390248311286
1109	25	0.26809264189353854
1110	1	0.038679267879007995
1110	2	-0.05123594093532091
1110	3	-0.029296188639072795
1110	4	0.02487906065110934
1110	5	0.06841382928411832
1110	6	0.061522057448549106
1110	7	0.12752500918223575
1110	8	0.09353736307251381
1110	9	0.03472925626758183
1110	10	0.08887920747262967
1110	11	0.09427944096874086
1110	12	0.12949983854066005
1110	13	0.11129358764354436
1110	14	0.07886029733317201
1110	15	0.04984351017624533
1110	16	0.12343596670991985
1110	17	0.07860373386150599
1110	18	0.1476952375556735
1110	19	0.1370699591066895
1110	20	0.10628634999763875
1110	21	0.11862348053496419
1110	22	0.09260098326674893
1110	23	0.16078854681808963
1110	24	0.0924129899141235
1110	25	0.3186954976041747
1111	1	-0.053317587334834826
1111	2	-0.09929704981228582
1111	3	-0.11518961218986234
1111	4	-0.06604286644326862
1111	5	-0.0938073960346344
1111	6	-0.014723712339794304
1111	7	-0.05573247322643482
1111	8	-0.07856636762110725
1111	9	-0.07603875954465877
1111	10	-0.06077534474695767
1111	11	-0.043223324743117075
1111	12	-0.08503165853273487
1111	13	-0.02447326741856415
1111	14	-0.09952934140466141
1111	15	-0.0726775196737961
1111	16	-0.09431897910576481
1111	17	-0.03287178555081738
1111	18	-0.07432374070781123
1111	19	-0.08483535302316625
1111	20	-0.020391685219323108
1111	21	-0.06472443349092316
1111	22	-0.12433495668675931
1111	23	-0.09712392156016847
1111	24	-0.14444248029695927
1111	25	0.018828552286940692
1112	1	-0.16533701697278502
1112	2	-0.10257939752939649
1112	3	-0.17340388862386646
1112	4	-0.13076222665601023
1112	5	-0.1193410607808687
1112	6	-0.01600553238022684
1112	7	-0.1206620585681506
1112	8	-0.052719529874131865
1112	9	-0.07638973629849488
1112	10	-0.10215142542108424
1112	11	-0.1175154094344748
1112	12	-0.08563960252801195
1112	13	-0.17680728189441908
1112	14	-0.13360100750950465
1112	15	-0.1562998536058302
1112	16	-0.1494281033838547
1112	17	-0.14574183775905425
1112	18	-0.050375736677771954
1112	19	-0.07933353442287938
1112	20	-0.15015454190338318
1112	21	-0.12429394736168575
1112	22	-0.08288908903051606
1112	23	-0.09436289624003535
1112	24	-0.0891489758806614
1112	25	0.031415185640661036
1113	1	-0.23944708860287278
1113	2	-0.24490547969165277
1113	3	-0.2997466343750368
1113	4	-0.24087988758365242
1113	5	-0.22865254207444252
1113	6	-0.1891895157295096
1113	7	-0.2807482400052663
1113	8	-0.26595863116104823
1113	9	-0.3015201612471401
1113	10	-0.2713729544258854
1113	11	-0.2566987747423042
1113	12	-0.23447144189706393
1113	13	-0.2467757286945158
1113	14	-0.28340129553807003
1113	15	-0.21675749361485686
1113	16	-0.30828754899911814
1113	17	-0.24203127353686682
1113	18	-0.26580217623295255
1113	19	-0.19272162787539315
1113	20	-0.19843616828944732
1113	21	-0.249115732638316
1113	22	-0.23434503224800807
1113	23	-0.19465602275641913
1113	24	-0.22682323341523158
1113	25	-0.16525148789109068
1114	1	-0.2605914015197257
1114	2	-0.28583268314165267
1114	3	-0.29391571947963513
1114	4	-0.20855985000208221
1114	5	-0.25015227665671347
1114	6	-0.22601583979409412
1114	7	-0.3098565927271437
1114	8	-0.3423499761200475
1114	9	-0.3318351546173446
1114	10	-0.4097529859369118
1114	11	-0.42414903883448574
1114	12	-0.3844154269385669
1114	13	-0.304547452658106
1114	14	-0.3921282037629297
1114	15	-0.48019687895342855
1114	16	-0.4761313838170267
1114	17	-0.391172103941102
1114	18	-0.4215472352923797
1114	19	-0.4766203699574376
1114	20	-0.4288583103643049
1114	21	-0.3485682754448405
1114	22	-0.34540184661399564
1114	23	-0.4666431070396442
1114	24	-0.5257354113492596
1114	25	-0.40025530979979607
1115	1	-0.37216861051216554
1115	2	-0.3374191859498339
1115	3	-0.3624684967485834
1115	4	-0.3357791865198507
1115	5	-0.30685485030634396
1115	6	-0.3427941271651136
1115	7	-0.3995069418970445
1115	8	-0.3602540280103881
1115	9	-0.510402206522329
1115	10	-0.4683244321934552
1115	11	-0.5138770126337135
1115	12	-0.3968190443584021
1115	13	-0.45400528457962963
1115	14	-0.6614318011352012
1115	15	-0.5524077909488128
1115	16	-0.5981846186821258
1115	17	-0.6664224573838027
1115	18	-0.660447413766569
1115	19	-0.5999235383203043
1115	20	-0.6402938716243298
1115	21	-0.6448998134557229
1115	22	-0.6167994432324733
1115	23	-0.6097188035289136
1115	24	-0.6947198132669428
1115	25	-0.5497020353296449
1116	1	-0.2340459925334173
1116	2	-0.2059701083125276
1116	3	-0.2180522224639212
1116	4	-0.18222359963939283
1116	5	-0.2404770474018747
1116	6	-0.24993645404590836
1116	7	-0.22083156228842427
1116	8	-0.3074712413077947
1116	9	-0.3302095441618036
1116	10	-0.31608056880098445
1116	11	-0.29710879109440924
1116	12	-0.2950970974392604
1116	13	-0.3343297327118435
1116	14	-0.3812965251124254
1116	15	-0.3945518279024114
1116	16	-0.3908335965693581
1116	17	-0.36614324981601953
1116	18	-0.2838704474707754
1116	19	-0.3715435030572536
1116	20	-0.3602994950848242
1116	21	-0.4031084758883523
1116	22	-0.42186341197344746
1116	23	-0.42865620735296517
1116	24	-0.4596455815853844
1116	25	-0.31057207680704124
1201	1	0.4190817088242831
1201	2	0.41915443953754306
1201	3	0.4278838804016196
1201	4	0.37122597427263315
1201	5	0.3388613735769894
1201	6	0.37269930653182115
1201	7	0.4462250794160951
1201	8	0.40938464373927275
1201	9	0.4765298791922703
1201	10	0.4651816798147479
1201	11	0.5113458255920733
1201	12	0.5467422670566364
1201	13	0.5182260316658756
1201	14	0.5313353277922559
1201	15	0.5962432540978639
1201	16	0.5769674080530152
1201	17	0.5895427259628221
1201	18	0.6249268639666212
1201	19	0.6504616730339947
1201	20	0.6194567526905246
1201	21	0.6143836457443896
1201	22	0.6437980993449415
1201	23	0.6241976723962921
1201	24	0.627360481844029
1201	25	0.5871693486757792
1202	1	0.3874356342817755
1202	2	0.4212765697470111
1202	3	0.4126009744826682
1202	4	0.27544687224069975
1202	5	0.26238716926864925
1202	6	0.3864165517587992
1202	7	0.36843849178318067
1202	8	0.41720729994354144
1202	9	0.48082065785612826
1202	10	0.5285628857112954
1202	11	0.5220403056473089
1202	12	0.565979980853605
1202	13	0.5311440572289996
1202	14	0.601507082319074
1202	15	0.535871431832625
1202	16	0.5961309300065238
1202	17	0.6011004995257565
1202	18	0.5875613164775686
1202	19	0.4805020016003283
1202	20	0.6032751760721098
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2210	10	-0.018580768442937767
2210	11	0.00755972669775673
2210	12	-0.0782858057097684
2210	13	-0.02322486351797437
2210	14	-0.028631579229923432
2210	15	-0.0017542167563604494
2210	16	0.01905122357037925
2210	17	-0.013372753247217872
2210	18	-0.011478639046352157
2210	19	1.8819154483651346E-5
2210	20	0.01602444287709659
2210	21	-0.02571744921807806
2210	22	-0.007187641938259048
2210	23	-0.03354747121029078
2210	24	-0.004351847378401885
2210	25	-0.16666262075187888
2211	1	-0.18765265744829307
2211	2	-0.15022838271537609
2211	3	-0.10434638756933978
2211	4	-0.07117442839157206
2211	5	-0.03841680698976546
2211	6	-0.13874341606239682
2211	7	-0.13799034965096268
2211	8	-0.20345683600172748
2211	9	-0.20584984377110516
2211	10	-0.1381435208985593
2211	11	-0.18979477522542254
2211	12	-0.21209580989948051
2211	13	-0.20936473554093737
2211	14	-0.19814780163487455
2211	15	-0.23536569500329563
2211	16	-0.1683249661057573
2211	17	-0.24248013572434246
2211	18	-0.22567804951120252
2211	19	-0.22436272026394022
2211	20	-0.22198633890543107
2211	21	-0.19610709601310106
2211	22	-0.2847625926039967
2211	23	-0.26724248938343464
2211	24	-0.30135318839987235
2211	25	-0.2868584587492222
2212	1	-0.3225000035961365
2212	2	-0.26624371627368504
2212	3	-0.2767625199697257
2212	4	-0.24011192896661981
2212	5	-0.1669404665492067
2212	6	-0.2468488771457089
2212	7	-0.2773514036650937
2212	8	-0.2744124129747405
2212	9	-0.29248875736993796
2212	10	-0.30196171168514496
2212	11	-0.3505758550561353
2212	12	-0.3376441664536216
2212	13	-0.25423731594453614
2212	14	-0.28067063713525187
2212	15	-0.2658462609742216
2212	16	-0.33386954574671535
2212	17	-0.3160086923417893
2212	18	-0.31442953151941566
2212	19	-0.24492468850714033
2212	20	-0.26700008336558767
2212	21	-0.2447395924138931
2212	22	-0.34324866824832156
2212	23	-0.3491535587370791
2212	24	-0.38577151881028726
2212	25	-0.41051220691487345
2213	1	-0.3495543403940786
2213	2	-0.31842046128348755
2213	3	-0.35032055390822237
2213	4	-0.2753875241727144
2213	5	-0.2827499623864753
2213	6	-0.34136300425101496
2213	7	-0.32152494262062703
2213	8	-0.3334402413608544
2213	9	-0.393996906887331
2213	10	-0.45309495804903577
2213	11	-0.4089037920948613
2213	12	-0.33871473332972357
2213	13	-0.3550750470487358
2213	14	-0.44461306811300005
2213	15	-0.47298041353182896
2213	16	-0.5039901471644157
2213	17	-0.41675705745947056
2213	18	-0.43592186143222733
2213	19	-0.3849799085132659
2213	20	-0.41025786285863575
2213	21	-0.40975489698720935
2213	22	-0.47999881243870135
2213	23	-0.43442162342271473
2213	24	-0.47877725960222506
2213	25	-0.44381650416176655
2214	1	-0.4029193190848055
2214	2	-0.46291626655285967
2214	3	-0.40716965825690465
2214	4	-0.2800815440226003
2214	5	-0.38345211809944857
2214	6	-0.4509748086977936
2214	7	-0.4858995436251661
2214	8	-0.549869937301267
2214	9	-0.5842368187901812
2214	10	-0.5951036218300203
2214	11	-0.5774987646506347
2214	12	-0.6534556421364215
2214	13	-0.6291352790844371
2214	14	-0.6865703817778837
2214	15	-0.6376102712369516
2214	16	-0.6383736459911376
2214	17	-0.6695026301433771
2214	18	-0.692584266926751
2214	19	-0.6691298620965682
2214	20	-0.6323155226900425
2214	21	-0.7269760682059783
2214	22	-0.6829300217959826
2214	23	-0.723619321128794
2214	24	-0.6983634837639698
2214	25	-1.5651215215624532
2215	1	-0.6058134336281498
2215	2	-0.6127173956857135
2215	3	-0.6035117906736502
2215	4	-0.49626573225459936
2215	5	-0.5888772904089379
2215	6	-0.576228264692972
2215	7	-0.5628384957972301
2215	8	-0.6081707471867901
2215	9	-0.7011317799065964
2215	10	-0.6946152651556545
2215	11	-0.7614670954279532
2215	12	-0.7421865160307703
2215	13	-0.7562655226731252
2215	14	-0.8361353327564487
2215	15	-0.8563184227628469
2215	16	-0.799474902232177
2215	17	-0.943222067717123
2215	18	-0.9097404879578193
2215	19	-0.8409455852052119
2215	20	-0.8868054304487387
2215	21	-0.8786753780372223
2215	22	-0.9065086558373423
2215	23	-0.8878891661148529
2215	24	-0.9040222330518404
2215	25	-0.9324945153487363
2216	1	-0.4137364533286032
2216	2	-0.29753090916094393
2216	3	-0.2841071236190409
2216	4	-0.24311044412859673
2216	5	-0.24099105326959602
2216	6	-0.32181729366018885
2216	7	-0.32388462314331434
2216	8	-0.3508402676626048
2216	9	-0.4071016217525596
2216	10	-0.37011192604384036
2216	11	-0.43564428157187507
2216	12	-0.4183422966985546
2216	13	-0.410749769888632
2216	14	-0.46768528084271566
2216	15	-0.5020582005977658
2216	16	-0.49646698106838016
2216	17	-0.483341332811392
2216	18	-0.42271708451506385
2216	19	-0.42331656081974955
2216	20	-0.4439620412547072
2216	21	-0.4984608917427309
2216	22	-0.5567879264952609
2216	23	-0.5737901506136538
2216	24	-0.5587065958869317
2216	25	-0.6774185769512613
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90310.0
3	8.0
4	1652.0
5	13696.0
6	16317.0
7	12694.0
8	10346.0
9	8916.0
10	8047.0
11	7786.0
12	7359.0
13	6988.0
14	7011.0
15	7266.0
16	7728.0
17	8427.0
18	9529.0
19	10989.0
20	13701.0
21	18015.0
22	23288.0
23	29202.0
24	35319.0
25	42370.0
26	50018.0
27	58240.0
28	69745.0
29	87830.0
30	115808.0
31	154669.0
32	207485.0
33	282838.0
34	395543.0
35	566192.0
36	852424.0
37	1527572.0
38	4890640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.025153220492165	18.61751870081993	13.369344547488692	25.987983531199216
2	16.231246209472424	21.0025158332201	38.20375390652186	24.562484050785613
3	18.82455306868823	24.55704585308581	28.580465968363516	28.03793510986245
4	13.168266718591456	17.210983089206245	37.01626927222889	32.604480919973405
5	15.133562478907345	35.91679740919515	33.51709797371697	15.43254213818054
6	32.25817218596866	37.171486696518585	16.665015060368972	13.905326057143782
7	28.853415498525408	30.607829276779274	21.669038490272623	18.869716734422692
8	26.018337022207728	34.281577943946026	19.927504217767858	19.77258081607839
9	27.02053546646598	15.167436746227454	19.115123207722146	38.69690457958443
10	16.15122674232382	26.947050212091696	31.323531300772412	25.578191744812074
11	36.10350382664281	21.85500044395252	23.108894353079325	18.93260137632534
12	25.03136153884485	23.858327582811402	29.109392383328807	22.000918495014943
13	29.684353256479667	20.650897189414458	24.724662126647498	24.94008742745838
14	23.08393375328294	21.000159182186756	25.38926880071602	30.526638263814288
15	24.392523374165087	28.490843537128924	22.536861620447908	24.579771468258084
16	24.424137193563705	26.64301534194565	24.906118809327896	24.026728655162756
17	24.285485110577152	26.211664410842577	25.24360880623736	24.259241672342906
18	25.417702672584568	24.766354946605475	26.740510655291523	23.07543172551843
19	26.73742290778097	24.766248189720596	24.875496687644365	23.62083221485407
20	25.871654437716863	25.28433758198202	25.15131875470185	23.692689225599267
21	25.68167309931661	24.976190841127146	24.692790391994652	24.64934566756159
22	25.09396781223132	25.585415275903	25.42854852637219	23.892068385493488
23	23.864094630111122	25.04746812174126	25.84486214412372	25.2435751040239
24	24.649169547037424	25.692627545406292	25.723955622872086	23.934247284684197
25	24.463537119647725	25.289537224821785	25.76025854147649	24.486667114054004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	866.0
1	866.0
2	1169.5
3	1473.0
4	1473.0
5	1473.0
6	3722.0
7	5971.0
8	5971.0
9	5971.0
10	9588.0
11	13205.0
12	13205.0
13	13205.0
14	23351.5
15	33498.0
16	33498.0
17	33498.0
18	62164.0
19	90830.0
20	90830.0
21	90830.0
22	147131.5
23	203433.0
24	203433.0
25	203433.0
26	293505.5
27	383578.0
28	383578.0
29	383578.0
30	480104.0
31	576630.0
32	576630.0
33	576630.0
34	671171.0
35	765712.0
36	765712.0
37	765712.0
38	830304.5
39	894897.0
40	894897.0
41	894897.0
42	968235.5
43	1041574.0
44	1041574.0
45	1041574.0
46	1085192.5
47	1128811.0
48	1128811.0
49	1128811.0
50	1121719.5
51	1114628.0
52	1114628.0
53	1114628.0
54	1022484.0
55	930340.0
56	930340.0
57	930340.0
58	860453.5
59	790567.0
60	790567.0
61	790567.0
62	701088.0
63	611609.0
64	611609.0
65	611609.0
66	520906.0
67	430203.0
68	430203.0
69	430203.0
70	343629.0
71	257055.0
72	257055.0
73	257055.0
74	212049.0
75	167043.0
76	167043.0
77	167043.0
78	139871.5
79	112700.0
80	112700.0
81	112700.0
82	86959.5
83	61219.0
84	61219.0
85	61219.0
86	44471.5
87	27724.0
88	27724.0
89	27724.0
90	19527.5
91	11331.0
92	11331.0
93	11331.0
94	6116.5
95	902.0
96	902.0
97	902.0
98	535.5
99	169.0
100	169.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03157632668210997
2	0.0033140126396442077
3	0.008792489784556039
4	0.16968780343928233
5	0.02210032179062731
6	0.09107320985322237
7	0.05350059155125618
8	0.02242136676509284
9	0.040751999178124866
10	0.02688492753911364
11	0.041787628128013676
12	0.033357608475918725
13	0.00672123188477841
14	0.0037800456670941743
15	0.0034072192451342007
16	0.004277147563040805
17	0.0022887399792542807
18	0.0030654616916708922
19	0.0
20	0.0019884075837865245
21	2.0712578997776298E-4
22	2.07125789977763E-5
23	1.4498805298443406E-4
24	9.631349233965978E-4
25	0.1533455786100368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9655968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.27503178200614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.21886992334572	39.69118558630143
2	11.668304777784318	11.26575567979344
3	2.655628115722183	3.8460159506303238
4	1.0103153698855105	1.9509202636428926
5	0.5337964587807861	1.288452050638239
6	0.3172847685711307	0.9190159372030678
7	0.2242672948883293	0.7578557551879047
8	0.1622843276404681	0.6267424859652075
9	0.1275767612612418	0.5542894984078671
>10	0.8614008685830586	8.149907886725547
>50	0.10461387356296536	3.529321695013409
>100	0.09092453908984947	8.999320456813415
>500	0.012529344877898408	4.139744272226046
>1k	0.011298010335432339	11.056444285632665
>5k	8.408824090287657E-4	2.812440836280379
>10k+	6.468326223298197E-5	0.41258735953826553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	15568	0.1612267149186907	No Hit
GTATCAACGCAGAGTACTTTTTTTT	13729	0.1421814985302354	No Hit
GAATAGGACCGCGGTTCTATTTTGT	10342	0.10710474599750124	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	9756	0.10103596035115278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0356289498888148E-5
2	0.0	0.0	0.0	0.0	1.0356289498888148E-5
3	0.0	0.0	0.0	0.0	1.0356289498888148E-5
4	0.0	0.0	0.0	0.0	1.0356289498888148E-5
5	0.0	0.0	0.0	1.0356289498888148E-5	1.0356289498888148E-5
6	0.0	0.0	0.0	1.0356289498888148E-5	1.0356289498888148E-5
7	0.0	0.0	0.0	3.1068868496664446E-5	1.0356289498888148E-5
8	0.0	0.0	0.0	4.142515799555259E-5	1.0356289498888148E-5
9	0.0	0.0	0.0	8.285031599110518E-5	1.0356289498888148E-5
10	0.0	0.0	0.0	9.320660548999334E-5	2.0712578997776296E-5
11	0.0	0.0	0.0	9.320660548999334E-5	2.0712578997776296E-5
12	0.0	0.0	0.0	9.320660548999334E-5	9.320660548999334E-5
13	0.0	0.0	0.0	1.0356289498888149E-4	1.1391918448776963E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4560	0.0	15.887109	1
GTATCAA	15430	0.0	13.604034	1
ACGCTTA	485	0.0	11.546112	17
CTCGACG	480	0.0	11.271496	13
AACCGCG	805	0.0	11.20642	7
GAGCGTT	500	0.0	11.015322	7
CGCCAGT	1190	0.0	11.006713	18
CGTCGTA	1535	0.0	10.948442	10
CGCTTAC	520	0.0	10.7689705	18
GCGTTAT	660	0.0	10.673647	1
CGTTATT	660	0.0	10.672206	2
CGAGCCG	1830	0.0	10.632601	15
ATCGCCA	1250	0.0	10.554593	16
CGAACGA	775	0.0	10.532548	16
GGTAGCG	445	0.0	10.454988	11
CCGTCGT	1675	0.0	10.203564	9
CGACCAT	1595	0.0	10.179416	10
TACCGTC	1690	0.0	10.170237	7
ATACCGT	1750	0.0	10.150905	6
GCATCGC	1295	0.0	10.114537	14
>>END_MODULE