FastQCFastQC Report
Thu 2 Feb 2017
SRR4050250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262956
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48400.3832279192624288No Hit
GTACGGAAGCAGTGGTATCAACGCA46350.3669961582192887No Hit
CTTCCGTACTCTGCGTTGATACCAC27720.21948508103211828No Hit
TATCAACGCAGAGTACTTTTTTTTT26640.21093371423865914No Hit
GAGTACGGAAGCAGTGGTATCAACG23730.18789253148961643No Hit
TCCGTACTCTGCGTTGATACCACTG22390.17728250231995415No Hit
GTACATGGGAAGCAGTGGTATCAAC18350.14529405616664398No Hit
GGTATCAACGCAGAGTACTTTTTTT18060.14299785582395586No Hit
CTATTGGAGCTGGAATTACCGCGGC18010.14260195921314758No Hit
GAATAGGACCGCGGTTCTATTTTGT17550.13895971039371127No Hit
GATTAAGAGGGACGGCCGGGGGCAT17540.1388805310715496No Hit
GTCCTATTCCATTATTCCTAGCTGC17450.13816791717209467No Hit
GTATCTGATCGTCTTCGAACCTCCG17330.13721776530615476No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16950.13420895106401173No Hit
CTTTAATATACGCTATTGGAGCTGG15690.12423235647164271No Hit
GCTTCCGTACTCTGCGTTGATACCA15580.12336138392786448No Hit
CTCTTAATCATGGCCTCAGTTCCGA14570.11536427238953693No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14300.11322643069117214No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC14150.11203874085874725No Hit
GAACTACGACGGTATCTGATCGTCT13690.10839649203931095No Hit
CCCATGTACTCTGCGTTGATACCAC13650.1080797747506643No Hit
GAATAACGCCGCCGCATCGCCAGTC13580.10752551949553271No Hit
GTCCTGTATTGTTATTTTTCGTCAC13390.10602111237446118No Hit
ATACAGGACTCTTTCGAGGCCCTGT13380.10594193305229953No Hit
ATCAGATACCGTCGTAGTTCCGACC13370.10586275373013787No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13310.10538767779716791No Hit
GGGTAGGCACACGCTGAGCCAGTCA13020.1030914774544798No Hit
GTACAAAGGGCAGGGACTTAATCAA12780.1011911737226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTGA851.0095391E-915.657495514
CGAACGA1006.530172E-1014.257816
CCAATTG759.587384E-713.94373813
TAGGACG852.849556E-713.3515354
GCGTTAT1001.0506483E-813.2591971
CGTTATT1001.07193046E-813.24027252
GTAACAC604.2493592E-412.6097833
TAAGACT854.145011E-612.2389074
GCGCGCT1900.012.00800515
TCAATAC803.00039E-511.8216713
ATACCGT2900.011.812746
CGTCGTA2850.011.68562610
ACGAACG1157.0025635E-811.57293115
GTAGGAC1157.5229764E-811.513283
CCGTCGT3000.011.4185269
GTCGTAG2950.011.28950311
TAGAACT1604.9112714E-1111.2305874
TCCATTA3650.011.2099698
AGGCCGG1201.2451528E-711.10178856
GACGGCC3950.011.0812711
Produced by FastQC (version 0.11.5)