##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050250_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1262956
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.334341022173376	31.0	31.0	34.0	26.0	34.0
2	30.469553967042398	31.0	31.0	34.0	27.0	34.0
3	30.573680318237532	31.0	31.0	34.0	27.0	34.0
4	34.14169693956084	37.0	35.0	37.0	30.0	37.0
5	34.14260354279959	37.0	35.0	37.0	30.0	37.0
6	34.11167847494291	37.0	35.0	37.0	30.0	37.0
7	34.000498037936396	37.0	35.0	37.0	30.0	37.0
8	33.92369726261247	37.0	35.0	37.0	30.0	37.0
9	35.423732893307445	39.0	37.0	39.0	30.0	39.0
10	35.304152322012804	39.0	35.0	39.0	30.0	39.0
11	35.150059067774336	39.0	35.0	39.0	28.0	39.0
12	35.05356560323558	39.0	35.0	39.0	27.0	39.0
13	35.082332242770136	39.0	35.0	39.0	28.0	39.0
14	36.22293413230548	40.0	37.0	41.0	27.0	41.0
15	36.193968752672305	40.0	37.0	41.0	27.0	41.0
16	36.19554679656299	40.0	37.0	41.0	28.0	41.0
17	36.163133157449664	40.0	37.0	41.0	27.0	41.0
18	36.15462217211051	40.0	37.0	41.0	28.0	41.0
19	36.156342738781085	40.0	37.0	41.0	27.0	41.0
20	36.088135295291366	40.0	37.0	41.0	27.0	41.0
21	36.00411178219986	40.0	37.0	41.0	27.0	41.0
22	35.927151856438385	40.0	37.0	41.0	27.0	41.0
23	35.84723220761452	40.0	36.0	41.0	27.0	41.0
24	35.77523999252547	40.0	36.0	41.0	27.0	41.0
25	35.597163321604235	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5845372253300276
1101	2	0.6336986087721144
1101	3	0.5663617082927317
1101	4	0.5150645852073907
1101	5	0.6087119032336332
1101	6	0.6769311356699603
1101	7	0.7287963665780097
1101	8	0.7095873264117287
1101	9	0.8271090586155836
1101	10	0.7705123209746318
1101	11	0.46296397187310134
1101	12	0.8201430180717253
1101	13	0.7706995534604317
1101	14	0.8668642063273211
1101	15	0.9800781889025103
1101	16	0.9970393866685896
1101	17	1.027365364044364
1101	18	0.9198581010508775
1101	19	1.039941591251491
1101	20	1.0560837665545861
1101	21	1.0996902515451268
1101	22	1.0647663693164162
1101	23	1.1331465359695798
1101	24	1.1766101553017236
1101	25	1.3261785926656415
1102	1	0.7430577903183178
1102	2	0.6999334838696925
1102	3	0.6744937828438644
1102	4	0.7080046710778021
1102	5	0.6963979151383981
1102	6	0.7552445870751114
1102	7	0.6193219569371067
1102	8	0.561231549674801
1102	9	0.6168735248528989
1102	10	0.6753028898661171
1102	11	0.5517288528254838
1102	12	0.6920222871974104
1102	13	0.8220363996743245
1102	14	0.846197978770725
1102	15	0.8521574970602046
1102	16	0.8864989153571159
1102	17	0.8867373146688777
1102	18	0.7976409611211395
1102	19	0.6923518176371317
1102	20	0.6502283557427191
1102	21	0.8352069377277971
1102	22	0.8519907057723088
1102	23	0.8739148566175103
1102	24	0.9054993287989888
1102	25	0.9537967864595558
1103	1	0.6563811562212827
1103	2	0.625565222414167
1103	3	0.5977375496902262
1103	4	0.6514851292988268
1103	5	0.6722339158122494
1103	6	0.6451472993392642
1103	7	0.6333991975031452
1103	8	0.6699411184079551
1103	9	0.6887000915644919
1103	10	0.7294197193809424
1103	11	0.8301697220977218
1103	12	0.7491798849063045
1103	13	0.697002706951956
1103	14	0.8552149935955242
1103	15	0.8420138818310932
1103	16	0.9564976518800279
1103	17	0.7938022573912491
1103	18	0.8063147313367693
1103	19	0.7869272893352459
1103	20	0.763678069354583
1103	21	0.6229680563261795
1103	22	0.670984978009507
1103	23	0.7728766996094194
1103	24	0.7876864076399599
1103	25	0.7708474097749161
1104	1	0.5215008287178904
1104	2	0.590759880217707
1104	3	0.643235391329366
1104	4	0.5667922650627659
1104	5	0.6696499626679326
1104	6	0.7008046367528777
1104	7	0.7952458626836147
1104	8	0.6176560931764143
1104	9	0.9067877635747053
1104	10	0.7351469755267033
1104	11	0.881188103631068
1104	12	0.8233788594315641
1104	13	0.8631883867849623
1104	14	1.050892191982328
1104	15	1.049068580572552
1104	16	1.0192400874838583
1104	17	1.0414726463015285
1104	18	0.9884094560190988
1104	19	1.00189800453294
1104	20	0.9735659967201613
1104	21	1.120501647717056
1104	22	1.1804513663523295
1104	23	1.0330473418484445
1104	24	1.094816091688358
1104	25	1.1694125557936061
1105	1	0.6753990470923661
1105	2	0.6698430691474293
1105	3	0.6789380757085333
1105	4	0.8996396627984069
1105	5	0.7345636770306143
1105	6	0.6857359172346804
1105	7	0.724277949146952
1105	8	0.7369851625003321
1105	9	0.8274482134348702
1105	10	0.8716414934714223
1105	11	0.9081343266324993
1105	12	0.8635721093786515
1105	13	1.014090065186899
1105	14	1.1133883012906765
1105	15	1.1550532849196031
1105	16	1.0254074704150682
1105	17	1.0598701001197242
1105	18	0.955087591973161
1105	19	0.9880295987601144
1105	20	1.154607377833642
1105	21	1.084395463243382
1105	22	1.1415506749127573
1105	23	1.109533529468628
1105	24	1.166328985016058
1105	25	1.0423751066838491
1106	1	0.33457767668195615
1106	2	0.47227926309047064
1106	3	0.33237121790880053
1106	4	0.28578064510377743
1106	5	0.384902379424112
1106	6	0.4131159344777089
1106	7	0.38902771992412255
1106	8	0.26134518603844015
1106	9	0.38625461251523774
1106	10	0.3126101138920916
1106	11	0.5205397294488563
1106	12	0.4372759397948158
1106	13	0.31745847759640355
1106	14	0.48716794630319527
1106	15	0.4728494613459162
1106	16	0.6236478601623077
1106	17	0.5364633698636823
1106	18	0.6429920163159437
1106	19	0.5255011682864819
1106	20	0.5304901252232384
1106	21	0.49209817149403534
1106	22	0.589563757720363
1106	23	0.47437143129283044
1106	24	0.575091455422367
1106	25	0.5346307961998136
1107	1	-0.11529245318817516
1107	2	-0.038889568078360526
1107	3	-0.021914496376915338
1107	4	-0.0913622120390798
1107	5	0.10853874306047118
1107	6	0.02298606434784034
1107	7	-0.28577747488107264
1107	8	-0.46384961915636325
1107	9	-0.5238752576146339
1107	10	-0.31440287312089055
1107	11	0.013007261916392565
1107	12	-0.018308475789602596
1107	13	-0.1355285353906055
1107	14	0.05807703721228563
1107	15	0.032849461345918485
1107	16	-0.08440408788963794
1107	17	-0.15548468208437072
1107	18	-0.07960538628146452
1107	19	0.060046622831933405
1107	20	0.16425635898947633
1107	21	0.039111158507019184
1107	22	0.27216116031776494
1107	23	0.15385195077335112
1107	24	0.200546000876912
1107	25	0.12787754944656626
1108	1	0.27543975608364946
1108	2	0.21139243371063543
1108	3	0.2958577365688022
1108	4	0.24451193970249818
1108	5	0.3278039108290045
1108	6	0.3710614172704254
1108	7	0.18368737409698355
1108	8	0.3062540213771072
1108	9	0.2552475977795865
1108	10	0.4432227431064817
1108	11	0.32419304143981975
1108	12	0.25750608768938577
1108	13	0.19705951870961513
1108	14	0.2643051539945205
1108	15	0.28010846852206583
1108	16	0.338816772037589
1108	17	0.4565478426151657
1108	18	0.4182544131630124
1108	19	0.4684347132500619
1108	20	0.27574882302466364
1108	21	0.29367705868659044
1108	22	0.29165580342711905
1108	23	0.2842525910445275
1108	24	0.4237399586539894
1108	25	0.3782122794498619
1109	1	0.07878197391377384
1109	2	0.03619522480459736
1109	3	0.024031199110066126
1109	4	-0.04836182812126566
1109	5	0.07649987469132924
1109	6	-0.07249985645918144
1109	7	-0.044964098654851625
1109	8	-0.24370055313573857
1109	9	-0.18697493062256143
1109	10	-0.0035740077966153194
1109	11	-0.14182705768857318
1109	12	-0.11997997452532161
1109	13	-0.30249942382938855
1109	14	-0.1528998350489914
1109	15	-0.16211194829439535
1109	16	-0.08526538765001845
1109	17	-0.0810803504323303
1109	18	-0.10045238841286874
1109	19	-0.09726701154591666
1109	20	-0.03020489840756113
1109	21	-0.10343302513808084
1109	22	0.04873555432335053
1109	23	0.06658849048236704
1109	24	0.18011945494577475
1109	25	0.023194016873127055
1110	1	-0.09365265507778986
1110	2	0.008944427990929427
1110	3	0.026314398910805892
1110	4	-0.02024848916065025
1110	5	0.07080340650161077
1110	6	-0.03847326064089174
1110	7	-0.058331195670092484
1110	8	-0.008471010836579751
1110	9	-0.10246425032126893
1110	10	0.09421151902291314
1110	11	0.04280308700633384
1110	12	-0.07375786300369214
1110	13	0.03814014886631156
1110	14	0.016389051994892156
1110	15	0.01602837393659229
1110	16	-0.1236761883382087
1110	17	-0.10056365539863776
1110	18	-0.2499166436515452
1110	19	-0.07316656920478692
1110	20	-0.11237472234748935
1110	21	-0.037118755369952794
1110	22	-0.13883046243856256
1110	23	-0.1418215260765976
1110	24	-0.04084816051724971
1110	25	-0.12858572584023875
1111	1	-0.2108347487304698
1111	2	-0.17431954350833578
1111	3	-0.0990643735267902
1111	4	-0.15368583436270455
1111	5	-0.047881053359319026
1111	6	-0.28948848812670747
1111	7	-0.2006809497845481
1111	8	-0.21653477184151626
1111	9	-0.06986332360269643
1111	10	-0.15688795560597413
1111	11	-0.1487477398386119
1111	12	-0.1482207257018544
1111	13	0.04244268258061368
1111	14	-0.10237224323699223
1111	15	-0.12208209358563948
1111	16	-0.06668559017114006
1111	17	-0.12945449605418702
1111	18	-0.16491251158858944
1111	19	-0.13489897211366042
1111	20	-0.07668432195120545
1111	21	-0.08616788677202436
1111	22	-0.08550819735159365
1111	23	-0.2965657396443362
1111	24	-0.1858071054761936
1111	25	0.003356664925682651
1112	1	0.1722300538033572
1112	2	0.2889624029594522
1112	3	0.16027018226794
1112	4	0.16047780634961128
1112	5	0.2299508412867297
1112	6	0.19617126655959538
1112	7	0.18173251758467046
1112	8	0.08471184887412875
1112	9	0.10731119604970019
1112	10	0.1317854321824541
1112	11	0.34536318524576615
1112	12	0.164432716155126
1112	13	0.1251074068382536
1112	14	0.19218780353543963
1112	15	0.16410385100738267
1112	16	0.28048474991955175
1112	17	0.3745049742769666
1112	18	0.30510722657274414
1112	19	0.264333486410834
1112	20	0.2532290185815995
1112	21	0.10898792873777552
1112	22	0.18089706551384666
1112	23	0.2017539799143222
1112	24	0.14930387034274162
1112	25	0.26670118402241627
1113	1	-0.3253696612035277
1113	2	-0.1952478489727234
1113	3	-0.23356264204567978
1113	4	-0.24205976191188938
1113	5	-0.22226502361958467
1113	6	-0.39830728148158556
1113	7	-0.3693888463274959
1113	8	-0.4528976844930881
1113	9	-0.34023443108720386
1113	10	-0.34948655232828685
1113	11	-0.23615237569685377
1113	12	-0.4633283356548219
1113	13	-0.4569584456864959
1113	14	-0.3342182276603012
1113	15	-0.3057857865676823
1113	16	-0.28577687317994815
1113	17	-0.3106558339565524
1113	18	-0.3524629754277129
1113	19	-0.2861398367565897
1113	20	-0.35900512179777877
1113	21	-0.19019039904195978
1113	22	-0.14183446604899785
1113	23	-0.40023025567998616
1113	24	-0.25366741457361996
1113	25	-0.15485909535193088
1114	1	-0.4732629331317497
1114	2	-0.3861613894308995
1114	3	-0.47543626110944004
1114	4	-0.42311898541020554
1114	5	-0.46587703548086523
1114	6	-0.731793247023397
1114	7	-0.6330617202819084
1114	8	-0.8564961355696994
1114	9	-1.0860849263713845
1114	10	-0.7261322100520218
1114	11	-0.6217893913555983
1114	12	-0.6926956531364183
1114	13	-0.5036851819329016
1114	14	-0.6610250664638073
1114	15	-0.6338599129867788
1114	16	-0.7711971951311725
1114	17	-0.6518761431395106
1114	18	-0.5951247050294768
1114	19	-0.6176657992751089
1114	20	-0.6879326677287025
1114	21	-0.8258220945876715
1114	22	-0.7854023026268635
1114	23	-0.7244159298263639
1114	24	-0.7771173721227882
1114	25	-0.6344126167897883
1115	1	-0.4592833729395984
1115	2	-0.46758716695461544
1115	3	-0.5266421202740226
1115	4	-0.6067608838646024
1115	5	-0.6297075398695355
1115	6	-0.6743025282763355
1115	7	-0.5893066063001129
1115	8	-0.6764490911474326
1115	9	-0.5746566732053182
1115	10	-0.5995823773443405
1115	11	-0.4972645811608558
1115	12	-0.5068569529039877
1115	13	-0.6050907645665475
1115	14	-0.6179998940650293
1115	15	-0.7221205324459063
1115	16	-0.8968164365260307
1115	17	-0.8097675117607324
1115	18	-0.7780101973081912
1115	19	-0.9318074911853884
1115	20	-0.7452378937399331
1115	21	-0.7206871696737309
1115	22	-0.7601865981526359
1115	23	-0.8576295469659883
1115	24	-0.8488520564013982
1115	25	-0.7306554385697623
1116	1	-0.7881339775973188
1116	2	-0.8178286075134196
1116	3	-0.7956584875284847
1116	4	-0.8265460174708963
1116	5	-0.8832706267928856
1116	6	-0.89260406382067
1116	7	-1.0600900504664637
1116	8	-1.1678842836028238
1116	9	-1.1484984397670601
1116	10	-1.0878614781564764
1116	11	-1.204529398895886
1116	12	-1.3169545933594478
1116	13	-1.12287552625655
1116	14	-1.350750242199922
1116	15	-1.3464272282723755
1116	16	-1.3997394591164465
1116	17	-1.183953041053428
1116	18	-1.4237122480673108
1116	19	-1.4376409960536236
1116	20	-1.5373586006881865
1116	21	-1.421491579887018
1116	22	-1.7020580692126472
1116	23	-1.6374153313128375
1116	24	-1.7917050611167795
1116	25	-1.8041120706665907
1201	1	0.7952913952777685
1201	2	0.7891314720169156
1201	3	0.7703443364932738
1201	4	0.685176380372873
1201	5	0.7012155422438227
1201	6	0.7879591262204642
1201	7	0.8529683426202013
1201	8	0.8513321423136091
1201	9	1.0991942711099156
1201	10	1.0629915010638769
1201	11	1.0954603328629133
1201	12	1.133565000079507
1201	13	1.100537647334967
1201	14	1.1254838880034157
1201	15	1.186186953548038
1201	16	1.1736679928680331
1201	17	1.272223025055652
1201	18	1.2014029503600625
1201	19	1.2869790790220321
1201	20	1.382310291956074
1201	21	1.3648291304913656
1201	22	1.3283923745184154
1201	23	1.376583193329921
1201	24	1.3136427512880715
1201	25	1.2956484902961094
1202	1	0.4869193974611754
1202	2	0.6770540195839772
1202	3	0.44960538998672206
1202	4	0.5372457425063715
1202	5	0.4860184508526828
1202	6	0.547097265646542
1202	7	0.5651924926513914
1202	8	0.6694904782462316
1202	9	0.8518065605671836
1202	10	0.6898118184375477
1202	11	0.6225993885397685
1202	12	0.7444776443402645
1202	13	0.7187997925314704
1202	14	0.721197978770725
1202	15	0.8940101756316281
1202	16	0.7135078439285465
1202	17	0.9051524932403083
1202	18	0.866837389692563
1202	19	0.8313027104942705
1202	20	0.9097149628855803
1202	21	0.8497158662992277
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2210	16	-0.19766335873926977
2210	17	-0.17675208937587428
2210	18	-0.16578282330227267
2210	19	-0.09998467117526388
2210	20	0.037171395759934
2210	21	0.024252763889592188
2210	22	-0.07092129939120184
2210	23	0.05413672623885191
2210	24	-0.001049993490170209
2210	25	0.0015686775955288113
2211	1	-0.5920043488874143
2211	2	-0.4591267992016661
2211	3	-0.47260051921483637
2211	4	-0.5705154638505618
2211	5	-0.6375068569091766
2211	6	-0.5342824898827345
2211	7	-0.4211049322338454
2211	8	-0.4526481623852874
2211	9	-0.4929823530508415
2211	10	-0.34825686722805216
2211	11	-0.4600480372148539
2211	12	-0.3711052992193373
2211	13	-0.3653766619475931
2211	14	-0.40936716415596663
2211	15	-0.3455202701883735
2211	16	-0.34845042130305615
2211	17	-0.4201154943101173
2211	18	-0.45672497555295166
2211	19	-0.39767287286224473
2211	20	-0.35605674562742706
2211	21	-0.5068064681700974
2211	22	-0.42119358620153946
2211	23	-0.41928428008439766
2211	24	-0.588776322369462
2211	25	-0.4412452265116542
2212	1	-0.15199103441230477
2212	2	-0.25622201665168376
2212	3	-0.25951679675293704
2212	4	-0.3104629166872286
2212	5	-0.15406181939410146
2212	6	-0.09223243977577056
2212	7	-0.1839314863197572
2212	8	-0.11371848508362348
2212	9	-0.09441780509148856
2212	10	-0.2771961868631152
2212	11	-0.18594113761594855
2212	12	-0.24541878640356174
2212	13	-0.3555140000476058
2212	14	-0.22972149029426703
2212	15	-0.2919332984733387
2212	16	-0.33767801590808944
2212	17	-0.2535767603224315
2212	18	-0.22982878818369556
2212	19	-0.35425489073965366
2212	20	-0.2255284547369456
2212	21	-0.2934372663041813
2212	22	-0.24578366857596734
2212	23	-0.2856879740220464
2212	24	-0.34216452460733393
2212	25	-0.3518039369502475
2213	1	-0.39610163447400026
2213	2	-0.5827443215231973
2213	3	-0.5011847970962151
2213	4	-0.4485176971640641
2213	5	-0.5345038578086303
2213	6	-0.4846325705920549
2213	7	-0.44149436047403157
2213	8	-0.3854310882116181
2213	9	-0.5901194233630846
2213	10	-0.5382917963997116
2213	11	-0.5116467685145665
2213	12	-0.6634911482172683
2213	13	-0.6395511667154281
2213	14	-0.6712538035389528
2213	15	-0.7209568848341164
2213	16	-0.6229781163397377
2213	17	-0.6830171496168376
2213	18	-0.7856319050439211
2213	19	-0.6274851018780581
2213	20	-0.7329070495752674
2213	21	-0.7599793791726484
2213	22	-0.7326498532635028
2213	23	-0.7163395736221787
2213	24	-0.7715033225356152
2213	25	-0.743303662096892
2214	1	-0.6699212765759128
2214	2	-0.6633602376845502
2214	3	-0.674471449647136
2214	4	-0.7664302348389143
2214	5	-0.680541544222379
2214	6	-0.6601836807905173
2214	7	-0.587923697654972
2214	8	-0.5436972085617313
2214	9	-0.5892937383255514
2214	10	-0.7113309474241447
2214	11	-0.8812585673565678
2214	12	-0.8195107162500435
2214	13	-0.7276587636977254
2214	14	-0.8066942542637108
2214	15	-0.9294764675991303
2214	16	-0.8583280431545361
2214	17	-0.9138778248427073
2214	18	-0.9121165175657708
2214	19	-0.748612620920035
2214	20	-0.8137142581252235
2214	21	-0.9127923065490862
2214	22	-0.9290862845816079
2214	23	-1.0020327161526836
2214	24	-0.93134647184295
2214	25	-0.9004508126335793
2215	1	-0.9856957341177726
2215	2	-0.9060666015711831
2215	3	-0.8659336351328939
2215	4	-0.8902822393801628
2215	5	-0.9373806007536061
2215	6	-0.9443960272447782
2215	7	-1.0655587462412939
2215	8	-1.0937881017264246
2215	9	-1.0748937128436111
2215	10	-1.2874582388078366
2215	11	-1.3521875432784114
2215	12	-1.1819721805059373
2215	13	-1.3052619598608715
2215	14	-1.3260241247836149
2215	15	-1.2467814340744567
2215	16	-1.3795783872744636
2215	17	-1.5897157142101364
2215	18	-1.612332659008736
2215	19	-1.8584086061496095
2215	20	-1.863959635542308
2215	21	-1.768024863365845
2215	22	-1.7997458800103274
2215	23	-1.9027987669299975
2215	24	-1.9876563231148836
2215	25	-1.9642755606012798
2216	1	-0.963912842383646
2216	2	-0.8709031774721616
2216	3	-0.8616804811908239
2216	4	-0.7745803357683911
2216	5	-0.9735640742500138
2216	6	-1.0397316652069506
2216	7	-1.1665973578734778
2216	8	-1.0721978204758145
2216	9	-1.2342557397522995
2216	10	-1.0917216565738599
2216	11	-1.1055587726879779
2216	12	-1.1035779666820957
2216	13	-1.072503764634206
2216	14	-1.296841015980526
2216	15	-1.368976189042037
2216	16	-1.5575744957565334
2216	17	-1.5084393312690523
2216	18	-1.647405892484521
2216	19	-1.4991417925865136
2216	20	-1.4271282308615696
2216	21	-1.5164375739107214
2216	22	-1.6439087123016307
2216	23	-1.6223714424468483
2216	24	-1.6869723593571067
2216	25	-1.8278935803820673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34614.0
3	1.0
4	333.0
5	1967.0
6	2984.0
7	3237.0
8	2556.0
9	2323.0
10	2332.0
11	3192.0
12	3434.0
13	3181.0
14	1681.0
15	1730.0
16	1769.0
17	2060.0
18	2100.0
19	2592.0
20	3370.0
21	4275.0
22	5193.0
23	6293.0
24	7366.0
25	8329.0
26	9439.0
27	10250.0
28	11745.0
29	13977.0
30	17366.0
31	22074.0
32	28543.0
33	37120.0
34	51033.0
35	71166.0
36	105287.0
37	191654.0
38	586390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09573410691664	17.394994712948396	12.2025785581302	25.30669262200476
2	16.812559941901398	20.4468111898413	37.808520125035535	24.932108743221765
3	18.656149585650347	25.691737797416565	28.229000753219	27.42311186371409
4	12.777162104035748	15.927290471350839	36.3172165878613	34.9783308367521
5	15.38085624242117	35.80502517498137	31.98129519081424	16.832823391783222
6	32.7254114921473	36.151567924925956	16.48258398211255	14.640436600814189
7	28.952114265248653	31.102511318921817	21.30690957381902	18.638464842010514
8	27.524047318879454	31.443171940211933	20.083740692287027	20.949040048621583
9	28.719771705270364	14.439705029519232	18.61002924986611	38.2304940153443
10	16.829694904298147	26.004825889327748	29.50113993019413	27.664339276179973
11	36.41216166677824	22.52571667192635	21.443735715173712	19.6183859461217
12	25.876050492107723	24.377309927921885	27.042312743037666	22.704326836932726
13	29.408369339438252	20.355292081363846	24.996397399121204	25.239941180076702
14	25.066808895115805	20.73483814216742	24.44548816067569	29.752864802041085
15	26.09156729334622	26.57077254032123	22.725586327802183	24.612073838530364
16	25.559032191562203	25.97763473246928	23.962866237424613	24.50046683854391
17	24.566045606007776	25.869381438867478	24.320232312666867	25.24434064245788
18	25.834784041493837	23.812628205587437	26.53207915732363	23.8205085955951
19	26.58790784915821	24.85154103549879	24.9128433026477	23.6477078126953
20	26.308763298983845	24.051090387317355	24.79778380121079	24.84236251248801
21	27.400408722491438	24.405684538523328	24.1780769025618	24.01582983642343
22	26.068108694994613	24.332994238573065	24.39476361274423	25.204133453688094
23	24.622560324671756	23.645456275111233	25.0597654281577	26.672217972059315
24	24.4380752565274	25.368912349291143	24.75182983708958	25.441182557091874
25	24.88176140877043	24.612411430499908	25.005770648175123	25.500056512554544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	252.0
1	252.0
2	490.0
3	728.0
4	728.0
5	728.0
6	1690.5
7	2653.0
8	2653.0
9	2653.0
10	3173.5
11	3694.0
12	3694.0
13	3694.0
14	4692.5
15	5691.0
16	5691.0
17	5691.0
18	8958.5
19	12226.0
20	12226.0
21	12226.0
22	17088.5
23	21951.0
24	21951.0
25	21951.0
26	32124.0
27	42297.0
28	42297.0
29	42297.0
30	50711.0
31	59125.0
32	59125.0
33	59125.0
34	70943.5
35	82762.0
36	82762.0
37	82762.0
38	90636.5
39	98511.0
40	98511.0
41	98511.0
42	110153.5
43	121796.0
44	121796.0
45	121796.0
46	137765.0
47	153734.0
48	153734.0
49	153734.0
50	164553.0
51	175372.0
52	175372.0
53	175372.0
54	152232.5
55	129093.0
56	129093.0
57	129093.0
58	119290.5
59	109488.0
60	109488.0
61	109488.0
62	99395.0
63	89302.0
64	89302.0
65	89302.0
66	76300.0
67	63298.0
68	63298.0
69	63298.0
70	49701.5
71	36105.0
72	36105.0
73	36105.0
74	30377.0
75	24649.0
76	24649.0
77	24649.0
78	20758.0
79	16867.0
80	16867.0
81	16867.0
82	12448.5
83	8030.0
84	8030.0
85	8030.0
86	5917.0
87	3804.0
88	3804.0
89	3804.0
90	2591.5
91	1379.0
92	1379.0
93	1379.0
94	750.0
95	121.0
96	121.0
97	121.0
98	74.5
99	28.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18440864131450344
2	0.02177431359445618
3	0.02969224581062206
4	0.04457795837701393
5	0.031117473609531925
6	0.06666898926011675
7	0.07205318316710954
8	0.07640804588600078
9	0.05764254653368763
10	0.04734923465267198
11	0.643569530529963
12	0.5728623958396016
13	0.5478417300365175
14	0.5353313971349755
15	0.5477625507143559
16	0.525196443898283
17	0.5319266862820241
18	0.5283636167847494
19	0.5451496330830211
20	0.5345396039133589
21	0.5421408188408781
22	0.5281260788182645
23	0.5380234940884718
24	0.519416353380482
25	0.522425167622625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1262956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.23431413599174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03351090989872	39.5683013490636
2	11.385100191100632	10.983049981745749
3	2.8712940151707875	4.154846925136224
4	1.1144439936309405	2.150177667030558
5	0.570130433454058	1.3749925212856082
6	0.3353311798215931	0.9704681680264473
7	0.22586502193129213	0.7626111094116624
8	0.1709513784850524	0.6596577993463069
9	0.12210473479255635	0.530067432192851
>10	0.8890384037306995	8.340218516230438
>50	0.12044923778833612	4.151278546195785
>100	0.13365914731694814	13.771115108864334
>500	0.02067746535170529	6.834891206818686
>1k	0.007443887526613903	5.74832366865174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4840	0.3832279192624288	No Hit
GTACGGAAGCAGTGGTATCAACGCA	4635	0.3669961582192887	No Hit
CTTCCGTACTCTGCGTTGATACCAC	2772	0.21948508103211828	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2664	0.21093371423865914	No Hit
GAGTACGGAAGCAGTGGTATCAACG	2373	0.18789253148961643	No Hit
TCCGTACTCTGCGTTGATACCACTG	2239	0.17728250231995415	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1835	0.14529405616664398	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1806	0.14299785582395586	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1801	0.14260195921314758	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1755	0.13895971039371127	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1754	0.1388805310715496	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1745	0.13816791717209467	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1733	0.13721776530615476	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1695	0.13420895106401173	No Hit
CTTTAATATACGCTATTGGAGCTGG	1569	0.12423235647164271	No Hit
GCTTCCGTACTCTGCGTTGATACCA	1558	0.12336138392786448	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1457	0.11536427238953693	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1430	0.11322643069117214	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1415	0.11203874085874725	No Hit
GAACTACGACGGTATCTGATCGTCT	1369	0.10839649203931095	No Hit
CCCATGTACTCTGCGTTGATACCAC	1365	0.1080797747506643	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1358	0.10752551949553271	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1339	0.10602111237446118	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1338	0.10594193305229953	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1337	0.10586275373013787	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1331	0.10538767779716791	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1302	0.1030914774544798	No Hit
GTACAAAGGGCAGGGACTTAATCAA	1278	0.1011911737226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.917932216165884E-5	0.0
10	0.0	0.0	0.0	1.5835864432331767E-4	0.0
11	0.0	0.0	0.0	1.5835864432331767E-4	0.0
12	0.0	0.0	0.0	1.5835864432331767E-4	0.0
13	0.0	0.0	0.0	1.5835864432331767E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTGA	85	1.0095391E-9	15.6574955	14
CGAACGA	100	6.530172E-10	14.2578	16
CCAATTG	75	9.587384E-7	13.943738	13
TAGGACG	85	2.849556E-7	13.351535	4
GCGTTAT	100	1.0506483E-8	13.259197	1
CGTTATT	100	1.07193046E-8	13.2402725	2
GTAACAC	60	4.2493592E-4	12.609783	3
TAAGACT	85	4.145011E-6	12.238907	4
GCGCGCT	190	0.0	12.008005	15
TCAATAC	80	3.00039E-5	11.821671	3
ATACCGT	290	0.0	11.81274	6
CGTCGTA	285	0.0	11.685626	10
ACGAACG	115	7.0025635E-8	11.572931	15
GTAGGAC	115	7.5229764E-8	11.51328	3
CCGTCGT	300	0.0	11.418526	9
GTCGTAG	295	0.0	11.289503	11
TAGAACT	160	4.9112714E-11	11.230587	4
TCCATTA	365	0.0	11.209969	8
AGGCCGG	120	1.2451528E-7	11.1017885	6
GACGGCC	395	0.0	11.08127	11
>>END_MODULE