FastQCFastQC Report
Thu 2 Feb 2017
SRR4050250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262956
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67070.5310557137382458No Hit
TATCAACGCAGAGTACTTTTTTTTT44770.3544858253177466No Hit
GTACGGAAGCAGTGGTATCAACGCA40650.3218639445871432No Hit
CTTCCGTACTCTGCGTTGATACCAC29600.23437079359851015No Hit
GGTATCAACGCAGAGTACTTTTTTT25670.20325331998897822No Hit
TCCGTACTCTGCGTTGATACCACTG23690.18757581420096978No Hit
GAGTACGGAAGCAGTGGTATCAACG23440.18559633114692833No Hit
GATTAAGAGGGACGGCCGGGGGCAT19320.15297445041632488No Hit
GAATAGGACCGCGGTTCTATTTTGT18890.14956973956337355No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18100.1433145731126025No Hit
CTATTGGAGCTGGAATTACCGCGGC17930.1419685246358543No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17900.1417309866693693No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16320.12922065376782724No Hit
GTACATGGGAAGCAGTGGTATCAAC16070.12724117071378577No Hit
GCTTCCGTACTCTGCGTTGATACCA15600.12351974257218779No Hit
GTATCTGATCGTCTTCGAACCTCCG15510.12280712867273286No Hit
CTTTAATATACGCTATTGGAGCTGG15510.12280712867273286No Hit
ATACAGGACTCTTTCGAGGCCCTGT15350.12154025951814632No Hit
ATCAGATACCGTCGTAGTTCCGACC15070.11932323849761987No Hit
CCCATGTACTCTGCGTTGATACCAC14660.11607688628899185No Hit
CTCTTAATCATGGCCTCAGTTCCGA14650.11599770696683019No Hit
GAATAACGCCGCCGCATCGCCAGTC14490.11473083781224366No Hit
GTCCTATTCCATTATTCCTAGCTGC14390.11393904459062708No Hit
GAACTACGACGGTATCTGATCGTCT13660.10815895407282597No Hit
TCGTAGTTCCGACCATAAACGATGC13620.10784223678417934No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13610.10776305746201767No Hit
ATATTAAAGTTGCTGCAGTTAAAAA13600.10768387813985601No Hit
GCGCAAGACGGACCAGAGCGAAAGC13300.10530849847500624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA1350.015.4710269
GTATTAA1101.7644197E-1013.867611
TTAGACC500.001480595313.3221433
AGGCCCG3300.012.94579810
CTCGTAG1650.012.658113510
TCTAGCG1501.8189894E-1212.6571117
CGCCAGT2500.012.5305418
CATCGCC2600.012.412230515
TGTACCG1155.316906E-912.3917695
GTTCAAA4100.012.3244151
TCGCCAG2550.012.284842517
ATCGCCA2550.012.284842516
CGCGCCA701.0974864E-412.206037510
TAAGGGT550.003029904312.111044
CGAACGA1650.012.08178816
AATCGGT550.003085286112.08178814
CCGCATC2600.012.04955112
GTCATAG802.7625234E-511.9174781
ATTCGCA802.8875755E-511.86604214
AAGCTCG1850.011.8051737
Produced by FastQC (version 0.11.5)