##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1262956
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.106448680714134	31.0	31.0	34.0	28.0	34.0
2	30.921787457361933	31.0	31.0	34.0	27.0	34.0
3	31.251818749030054	33.0	31.0	34.0	27.0	34.0
4	34.88441244192197	37.0	35.0	37.0	32.0	37.0
5	34.79003227349171	37.0	35.0	37.0	32.0	37.0
6	34.80346583728966	37.0	35.0	37.0	32.0	37.0
7	34.71425924576945	37.0	35.0	37.0	32.0	37.0
8	34.70119782478566	37.0	35.0	37.0	32.0	37.0
9	36.30453079917273	39.0	37.0	39.0	32.0	39.0
10	36.21460763478696	39.0	37.0	39.0	32.0	39.0
11	36.21793316631775	39.0	37.0	39.0	32.0	39.0
12	36.1105755069852	39.0	37.0	39.0	32.0	39.0
13	36.11496441681262	39.0	37.0	39.0	32.0	39.0
14	37.343107756723114	40.0	38.0	41.0	32.0	41.0
15	37.33689059634698	40.0	38.0	41.0	32.0	41.0
16	37.32799796667501	40.0	38.0	41.0	32.0	41.0
17	37.27382505803844	40.0	38.0	41.0	32.0	41.0
18	37.24813849413598	40.0	38.0	41.0	32.0	41.0
19	37.29656456757005	40.0	38.0	41.0	32.0	41.0
20	37.21507004202839	40.0	38.0	41.0	31.0	41.0
21	37.15318190024039	40.0	38.0	41.0	31.0	41.0
22	37.07673505648653	40.0	37.0	41.0	31.0	41.0
23	37.009083451838386	40.0	37.0	41.0	31.0	41.0
24	36.945248290518435	40.0	37.0	41.0	31.0	41.0
25	36.76676542967451	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4948144870861597
1101	2	0.8849129967257383
1101	3	0.7997034852017677
1101	4	0.7013142369951169
1101	5	0.7607556338799526
1101	6	0.8695382625518704
1101	7	0.827553284998011
1101	8	0.9188796991935533
1101	9	0.9754861160310355
1101	10	0.8775589024229475
1101	11	0.952775607209162
1101	12	0.9914824572244783
1101	13	0.9531057059307457
1101	14	1.0091880221905072
1101	15	1.055011349545282
1101	16	0.9633592335851233
1101	17	1.2061450842947394
1101	18	1.0021223645188542
1101	19	1.1266340281577456
1101	20	0.9878548166987215
1101	21	0.7419774099795973
1101	22	0.9935775960811171
1101	23	1.0362384664764264
1101	24	1.1265278806388537
1101	25	1.0134750191548179
1102	1	0.48737706027117156
1102	2	0.805578614407235
1102	3	0.6800813638122278
1102	4	0.4845688964408623
1102	5	0.5345486910618433
1102	6	0.5385657557212724
1102	7	0.49048446669200274
1102	8	0.5267220830733379
1102	9	0.7681432198561708
1102	10	0.634898749222323
1102	11	0.7270596138446024
1102	12	0.6673259144844366
1102	13	0.6814527004662665
1102	14	0.702567218131172
1102	15	0.8657847931440372
1102	16	0.6890038715632656
1102	17	0.8742760610706952
1102	18	0.8193001065172894
1102	19	0.7785624532162956
1102	20	0.8359135842631247
1102	21	0.9233908565057476
1102	22	0.9499855537313877
1102	23	0.9618983513320103
1102	24	0.9993000069073901
1102	25	1.0104774830502166
1103	1	0.45353482644367915
1103	2	0.7614937929786656
1103	3	0.6397385403620923
1103	4	0.5288811437454441
1103	5	0.56763705022626
1103	6	0.44938743698811834
1103	7	0.4934304741044002
1103	8	0.5356148559843916
1103	9	0.6371964543575217
1103	10	0.5380806056913272
1103	11	0.46116086047533145
1103	12	0.6105094555895576
1103	13	0.6376858541050794
1103	14	0.7609061669182395
1103	15	0.651526454195249
1103	16	0.7142334032344237
1103	17	0.7087373986717722
1103	18	0.7267419865711986
1103	19	0.6849345893348939
1103	20	0.7400177790070614
1103	21	0.7742752904680117
1103	22	0.736674822599312
1103	23	0.8611276303077489
1103	24	0.9375054482820531
1103	25	0.727679723616248
1104	1	0.3176209176825573
1104	2	0.7093992709593309
1104	3	0.546094051782152
1104	4	0.38497420659123804
1104	5	0.5678336333282203
1104	6	0.5038206899317998
1104	7	0.5232239605158497
1104	8	0.4089849388735516
1104	9	0.4644039609947299
1104	10	0.4959814224231778
1104	11	0.49448878946221697
1104	12	0.49445652001612217
1104	13	0.5558485114222265
1104	14	0.7099181848336471
1104	15	0.6579143916939785
1104	16	0.5732387332711042
1104	17	0.6764754770212846
1104	18	0.6338392445409227
1104	19	0.6268858286190877
1104	20	0.6729545684199465
1104	21	0.6541407222415145
1104	22	0.6797913740858448
1104	23	0.7997491166381323
1104	24	0.7772395544369317
1104	25	0.9169162921264729
1105	1	0.3149616653065479
1105	2	0.624131155616027
1105	3	0.42155852962586593
1105	4	0.3702139128114794
1105	5	0.5570047773556865
1105	6	0.5378454251101061
1105	7	0.5469789674823105
1105	8	0.5767294921881927
1105	9	0.54952493745364
1105	10	0.549091202744826
1105	11	0.5099666682627841
1105	12	0.6572583386527313
1105	13	0.5823352083693223
1105	14	0.6277281017680849
1105	15	0.5779495012954712
1105	16	0.6034491758309173
1105	17	0.7269458121244341
1105	18	0.6514483734900409
1105	19	0.7246958361005369
1105	20	0.7183044703462187
1105	21	0.776857381419191
1105	22	0.7449218353436109
1105	23	0.8181250655424535
1105	24	0.6791245167297646
1105	25	0.7021953862942283
1106	1	0.3227727583231186
1106	2	0.6659093773942502
1106	3	0.5058889936823583
1106	4	0.44496053605124786
1106	5	0.26096711638651726
1106	6	0.469931421305688
1106	7	0.36187448292577074
1106	8	0.48738157982658237
1106	9	0.5658217912847405
1106	10	0.4835939602612811
1106	11	0.5414549060523939
1106	12	0.661987034614306
1106	13	0.6736280251415891
1106	14	0.6048561791701346
1106	15	0.832795750936242
1106	16	0.7390018423424891
1106	17	0.7956376682135513
1106	18	0.8338333857380675
1106	19	0.7925356675020083
1106	20	0.735489477120268
1106	21	0.7810775448174354
1106	22	0.8233398556794427
1106	23	0.7806767604229208
1106	24	0.8252192439203796
1106	25	0.8012303239593024
1107	1	-0.04242204687168538
1107	2	0.3905847020695745
1107	3	0.1739409417343083
1107	4	0.19976573085644134
1107	5	0.22148659690599715
1107	6	0.31356778494205173
1107	7	0.2548614959127846
1107	8	0.25777119021619654
1107	9	0.2764711419340955
1107	10	0.18904850571583154
1107	11	0.1980782826757732
1107	12	0.17990911253638586
1107	13	0.09648516799873619
1107	14	0.18719384150779916
1107	15	0.10967886781936187
1107	16	0.24237846571910637
1107	17	0.2133000058758867
1107	18	-0.017595185690503
1107	19	0.033055148021489345
1107	20	0.3396453212761088
1107	21	0.2896489733888643
1107	22	0.14282037515995682
1107	23	-0.03698557723942031
1107	24	-0.01789763919650511
1107	25	0.15187967460865792
1108	1	0.19967309258233712
1108	2	0.6500045572014947
1108	3	0.4216562093770726
1108	4	0.350175921390921
1108	5	0.33998438084137916
1108	6	0.29772349458714586
1108	7	0.17362103776916626
1108	8	0.3153253812349135
1108	9	0.34399446273550893
1108	10	0.44512065728254413
1108	11	0.3116677693154912
1108	12	0.43118837703159585
1108	13	0.5204286196791088
1108	14	0.4811539052658915
1108	15	0.5665201518589882
1108	16	0.41295074170826496
1108	17	0.4877231901488912
1108	18	0.530555493252514
1108	19	0.46849630965633793
1108	20	0.6107546986930998
1108	21	0.6833170143070362
1108	22	0.5672074432963825
1108	23	0.6403836614969833
1108	24	0.461249831665107
1108	25	0.3612704081630369
1109	1	-0.03384479334030388
1109	2	0.3550572122301574
1109	3	0.17372965455054157
1109	4	0.1883281572170361
1109	5	0.2239765025151712
1109	6	0.23473436555817528
1109	7	0.07176711832298821
1109	8	0.11854882236997355
1109	9	0.05410779681845668
1109	10	0.11740282214353925
1109	11	0.004196873565000203
1109	12	0.045365246616370314
1109	13	-0.0489881761902069
1109	14	-0.08841228529098544
1109	15	-0.12911324730303164
1109	16	-0.05902508485875302
1109	17	-0.11852863439311534
1109	18	-0.16151962007365483
1109	19	-0.3396967484001294
1109	20	-0.17335572456897097
1109	21	-0.17948112179627884
1109	22	-0.14479575997911098
1109	23	0.012453770365063122
1109	24	0.057250963475034666
1109	25	0.07137846328175357
1110	1	-0.00395332605002352
1110	2	0.24177821443955594
1110	3	0.12899738855545806
1110	4	0.168116073324434
1110	5	0.1953788484453085
1110	6	0.19574469300130914
1110	7	0.24446927375585403
1110	8	0.26856196806579646
1110	9	0.28575571357160356
1110	10	0.1619344433076506
1110	11	0.11443852374189589
1110	12	0.20002781736096864
1110	13	0.3013307799031679
1110	14	0.27323041930907976
1110	15	0.18243513116385657
1110	16	0.21087219421283976
1110	17	0.2756692705980939
1110	18	0.2487575465445815
1110	19	0.1350796594577659
1110	20	0.17765280398947425
1110	21	0.2744881864633726
1110	22	0.09400618281635786
1110	23	0.21238596624976935
1110	24	0.42428109709987893
1110	25	0.31002819275717286
1111	1	-0.19103559548523208
1111	2	0.13713436861923967
1111	3	-0.11736126956790471
1111	4	-0.08929007819936174
1111	5	-0.13788160246220116
1111	6	-0.1974466060723401
1111	7	-0.20249196144067838
1111	8	-0.2928360403910375
1111	9	-0.14771980225685155
1111	10	-0.05924914258181957
1111	11	-0.19638995179246166
1111	12	-0.13882728620001927
1111	13	-0.2126197811062127
1111	14	-0.22148288716893205
1111	15	-0.22106174292125047
1111	16	-0.20747762413697757
1111	17	-0.1811120685361871
1111	18	-0.2327724408677554
1111	19	-0.07144467647833608
1111	20	-0.17138310975231974
1111	21	-0.12746580372591154
1111	22	-0.09927158693200511
1111	23	-0.12887746913131082
1111	24	-0.048715470014336404
1111	25	-0.09376441103542987
1112	1	-0.02243328440504655
1112	2	0.20846668242943167
1112	3	0.06117970100846293
1112	4	0.01140015346431511
1112	5	0.08122889983414439
1112	6	0.17599892312540533
1112	7	0.1286397334078302
1112	8	0.05201476375180647
1112	9	0.005759984851252398
1112	10	0.004749127611141546
1112	11	0.09124458539959335
1112	12	0.08535063266816678
1112	13	0.14722212032860682
1112	14	0.1286409293265649
1112	15	-0.0036858795276870637
1112	16	0.003021048308596619
1112	17	0.033303070657716205
1112	18	0.16056007104882752
1112	19	0.09583950231026961
1112	20	0.16100148723404573
1112	21	0.19901111567136098
1112	22	0.2527487358847793
1112	23	0.322896428660286
1112	24	0.2550850320162539
1112	25	0.33627431634842964
1113	1	-0.32106711314561664
1113	2	-0.24537905925501136
1113	3	-0.30160509298698557
1113	4	-0.082224718556688
1113	5	-0.2116869649273596
1113	6	-0.16562353919772477
1113	7	-0.14949428576258583
1113	8	-0.23532650246912823
1113	9	-0.25147815962345277
1113	10	-0.2641313041650122
1113	11	-0.20232873298897402
1113	12	-0.31553606084693797
1113	13	-0.20075765790388544
1113	14	-0.17646751431850305
1113	15	-0.20205585793598146
1113	16	-0.22824767789672507
1113	17	-0.20823076575797472
1113	18	-0.1601542074247817
1113	19	-0.18755269774099048
1113	20	-0.04921351747963598
1113	21	-0.2605576584672562
1113	22	-0.24606524094215132
1113	23	-0.2736080612845839
1113	24	-0.3274883920862237
1113	25	-0.25596251756343236
1114	1	-0.4495115194320114
1114	2	-0.8117854439777012
1114	3	-0.477966473222434
1114	4	-0.4298431057479064
1114	5	-0.3153697306229759
1114	6	-0.6341831387729968
1114	7	-0.48968065849484077
1114	8	-0.47721920055894884
1114	9	-0.4033347323112437
1114	10	-0.7080635501406221
1114	11	-0.5609329701471424
1114	12	-0.5073470493386125
1114	13	-0.4808230842870955
1114	14	-0.5073565308901067
1114	15	-0.6201697788005873
1114	16	-0.6906264068373318
1114	17	-0.5386750942606469
1114	18	-0.5620832437211618
1114	19	-0.496871407734659
1114	20	-0.5216733102667348
1114	21	-0.5866020700370669
1114	22	-0.5276415794275593
1114	23	-0.6862132802787855
1114	24	-0.46922060621453454
1114	25	-0.5967212610774837
1115	1	-0.7713038844932605
1115	2	-1.11454952632878
1115	3	-0.7506225102651669
1115	4	-0.6247519436467428
1115	5	-0.6430228000225213
1115	6	-0.8334559880015675
1115	7	-0.7282221514876213
1115	8	-0.7034373356857131
1115	9	-0.8408168236010951
1115	10	-0.9140098072893679
1115	11	-0.9437348824304905
1115	12	-0.8815621636524185
1115	13	-0.8031863627882032
1115	14	-0.9633787534491489
1115	15	-0.9922496816611002
1115	16	-1.064336528605736
1115	17	-1.1291246964136121
1115	18	-1.0784411287382767
1115	19	-1.1280437627249356
1115	20	-1.2112332931080587
1115	21	-1.2027656327678997
1115	22	-1.29788873707016
1115	23	-1.184740285110017
1115	24	-1.1967057938617316
1115	25	-1.2155199754756865
1116	1	-0.8483047930445586
1116	2	-1.3589688047885673
1116	3	-0.9538320620517986
1116	4	-0.5768899765620148
1116	5	-0.6762146349459144
1116	6	-0.772082568448127
1116	7	-0.776769203757226
1116	8	-0.6524554292102351
1116	9	-0.8651481933398983
1116	10	-0.8447396060320429
1116	11	-0.9605276037167343
1116	12	-0.9117540394730526
1116	13	-1.137598539589888
1116	14	-1.1622821200598779
1116	15	-1.22505595281158
1116	16	-1.2560216461016864
1116	17	-1.327242203435624
1116	18	-1.340357645504902
1116	19	-1.4692518851418797
1116	20	-1.3979472499336794
1116	21	-1.303653690255402
1116	22	-1.3486643497548059
1116	23	-1.579334664745268
1116	24	-1.6335926601325141
1116	25	-1.5220632846314501
1201	1	0.5858374665406672
1201	2	0.9434962882999365
1201	3	0.6860906042988155
1201	4	0.6324337397034938
1201	5	0.5494353293962178
1201	6	0.5991723332321897
1201	7	0.6069725944212578
1201	8	0.447658900758924
1201	9	0.6110744425635914
1201	10	0.6432692849366077
1201	11	0.5922976441003698
1201	12	0.5808839891881163
1201	13	0.6255029188068804
1201	14	0.7200149723420282
1201	15	0.7008425949219514
1201	16	0.6682444556244036
1201	17	0.7481673001536677
1201	18	0.8140511592309494
1201	19	0.7178413443579075
1201	20	0.8112942182306853
1201	21	0.8730652950584599
1201	22	0.9421559958711256
1201	23	0.8249139860793591
1201	24	0.9195350154850601
1201	25	0.8319182386646844
1202	1	0.5487609888426022
1202	2	0.7966500429786656
1202	3	0.6722688638122278
1202	4	0.3724037178694317
1202	5	0.23320940534755863
1202	6	0.3097711128641265
1202	7	0.25555143097771804
1202	8	0.329177440216192
1202	9	0.35296464842760145
1202	10	0.34472017779375363
1202	11	0.21255068527317178
1202	12	0.44243752162729066
1202	13	0.4526580576091206
1202	14	0.37555828955974846
1202	15	0.4333071145726066
1202	16	0.5121065501346962
1202	17	0.4931376682135493
1202	18	0.5430724279458587
1202	19	0.4509954889305803
1202	20	0.4536591199774094
1202	21	0.557319427934317
1202	22	0.4053426965885336
1202	23	0.5751796013320103
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2210	21	-0.06633913488840193
2210	22	-0.17125879867825233
2210	23	-0.20035907121532404
2210	24	-0.1350811785487167
2210	25	-0.1867527772768085
2211	1	-0.20700682917798474
2211	2	-0.41822522218338776
2211	3	-0.3461316439078992
2211	4	-0.283678925752767
2211	5	-0.38897433456759245
2211	6	-0.26763936234104335
2211	7	-0.2482151742524934
2211	8	-0.20720029399579687
2211	9	-0.45413647829378334
2211	10	-0.2765213674489786
2211	11	-0.2893423979094649
2211	12	-0.3762079349730669
2211	13	-0.21283892562915696
2211	14	-0.35579886199123223
2211	15	-0.34409368873800616
2211	16	-0.3135787416305007
2211	17	-0.21484501366153808
2211	18	-0.34266935203049087
2211	19	-0.40682168400724095
2211	20	-0.3044576643458612
2211	21	-0.30128616741969694
2211	22	-0.3033501631624773
2211	23	-0.39920563566200684
2211	24	-0.34456464307869794
2211	25	-0.3305105933512351
2212	1	-0.25757672188037617
2212	2	-0.874933040528461
2212	3	-0.4351455435565583
2212	4	-0.1331239585295947
2212	5	-0.03930020753043806
2212	6	-0.008524040507438713
2212	7	-0.07715733683598103
2212	8	-0.06487045038164752
2212	9	0.023784947350087293
2212	10	0.0363958056178717
2212	11	0.074182810751104
2212	12	0.08766529795680356
2212	13	0.1584904133616405
2212	14	0.11572545587883099
2212	15	0.11721070338039397
2212	16	0.09126398409367908
2212	17	-1.1394613933088635
2212	18	-0.4248729687347108
2212	19	-0.2559314857826607
2212	20	-0.0421399980274586
2212	21	-0.007310612037876751
2212	22	0.028618081103651605
2212	23	-0.04477146531728238
2212	24	0.11449405669884527
2212	25	0.1033278371517099
2213	1	-0.5232745594687174
2213	2	-0.914183723022969
2213	3	-0.6185991397285591
2213	4	-0.49141003332218247
2213	5	-0.5526852179331172
2213	6	-0.42102001048463933
2213	7	-0.5694498457484158
2213	8	-0.5773316287951076
2213	9	-0.6621177429655631
2213	10	-0.4185753289814116
2213	11	-0.4938469893829591
2213	12	-0.5633300886818446
2213	13	-0.5671769468168577
2213	14	-0.7222293568381843
2213	15	-0.8492685527295833
2213	16	-0.7183074070353967
2213	17	-1.4683307370703886
2213	18	-1.20198823782426
2213	19	-1.0614967443187737
2213	20	-0.8293559539024784
2213	21	-0.7439602772270604
2213	22	-0.6210009512733805
2213	23	-0.5870332416817448
2213	24	-0.47607673369590486
2213	25	-0.5117923445066452
2214	1	-0.7876703316349492
2214	2	-2.383605045415468
2214	3	-1.2083544803899322
2214	4	-0.8404395417596717
2214	5	-0.617963292244319
2214	6	-0.4946401554770148
2214	7	-0.6130896684720497
2214	8	-0.6394857896085426
2214	9	-0.8567527620744073
2214	10	-0.6905989984680545
2214	11	-0.7215007973027383
2214	12	-0.7153828327983618
2214	13	-0.6075094963953589
2214	14	-0.7803632043944688
2214	15	-0.8500158169537855
2214	16	-0.8040027707334545
2214	17	-0.8578532631090496
2214	18	-0.9120907424399789
2214	19	-0.8391143562735479
2214	20	-0.7999034989003846
2214	21	-0.9751480080415007
2214	22	-0.8608668743475505
2214	23	-0.7882932329693304
2214	24	-0.7330973230124513
2214	25	-0.8786034323937102
2215	1	-1.018088485696019
2215	2	-2.3379593854218825
2215	3	-1.36778154630397
2215	4	-0.9640346792597541
2215	5	-0.8502700674822421
2215	6	-0.9449831378193991
2215	7	-1.0243592833079944
2215	8	-1.0576491788883828
2215	9	-1.0327838136366552
2215	10	-1.02790296386722
2215	11	-1.1440816626420727
2215	12	-1.1152788573884322
2215	13	-1.0994683521653101
2215	14	-1.2393543471456567
2215	15	-1.1654817610254824
2215	16	-1.2292414932692708
2215	17	-1.3876295908431828
2215	18	-1.4400012007280978
2215	19	-1.4810528492443993
2215	20	-1.385194049879047
2215	21	-1.4575964128038876
2215	22	-1.3708091532064088
2215	23	-1.3786123714977947
2215	24	-1.4736734423407256
2215	25	-1.4341627040653009
2216	1	-0.9186429505686284
2216	2	-2.0563975808232513
2216	3	-1.2003207071599284
2216	4	-0.948447206366076
2216	5	-1.0322854457478314
2216	6	-0.7870222320696882
2216	7	-0.8462214467037086
2216	8	-0.8179567878759997
2216	9	-0.9837537450000582
2216	10	-1.0573811030141016
2216	11	-1.1373252275520187
2216	12	-1.1609862859947953
2216	13	-1.0530007454488413
2216	14	-1.1798066978889068
2216	15	-1.0979357539805648
2216	16	-1.1116632752874906
2216	17	-1.2252842852323624
2216	18	-1.2847940571066232
2216	19	-1.2256843233697339
2216	20	-1.2110737360244173
2216	21	-1.4138338696466946
2216	22	-1.3418350333293176
2216	23	-1.3788386123831913
2216	24	-1.3636924194960756
2216	25	-1.4109749787252284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14581.0
3	1.0
4	251.0
5	2182.0
6	3124.0
7	2260.0
8	1730.0
9	1470.0
10	1419.0
11	1437.0
12	1251.0
13	1082.0
14	1105.0
15	1162.0
16	1235.0
17	1339.0
18	1479.0
19	1937.0
20	2564.0
21	3365.0
22	4048.0
23	5037.0
24	5957.0
25	6879.0
26	7854.0
27	9168.0
28	10470.0
29	12491.0
30	16277.0
31	21387.0
32	28499.0
33	37781.0
34	52672.0
35	74831.0
36	110560.0
37	197568.0
38	616503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.075888186154394	18.247845721706035	12.872045824670344	25.80422026746923
2	16.367169528340508	21.183618814433196	36.96480707358579	25.48440458364051
3	18.718783552237767	25.405952384722823	28.385074381055237	27.490189681984173
4	13.131550250807939	16.52421833178024	35.87881912088348	34.46541229652834
5	15.224051101222472	36.08932731987144	31.999344043590998	16.687277535315094
6	32.74899161042054	36.62147697480749	16.151346777471336	14.478184637300629
7	28.628463063037373	31.349008312777155	21.251289217154074	18.771239407031402
8	27.33587938615944	31.729325332961615	20.076381584783242	20.858413696095703
9	28.28619284636676	14.540098121903993	18.65472414598702	38.51898488574223
10	16.745816614441164	26.284117824536562	29.640408206253916	27.32965735476836
11	36.53895955277036	22.357443175653575	21.43426780717934	19.66932946439673
12	25.77136295240358	24.616535994297934	27.076423536865445	22.53567751643304
13	29.599808048501014	20.18586986733649	25.02203403654839	25.19228804761411
14	24.90412232431401	20.516278417842155	24.793812514501422	29.785786743342413
15	26.062186791581844	26.521592130090415	22.811747537683985	24.604473540643752
16	25.61544448746545	25.798428915181848	23.995901655569625	24.59022494178308
17	24.54883622232287	25.760042313429764	24.630707641438025	25.060413822809345
18	25.813092459278074	23.50105625215764	26.962855396387525	23.722995892176765
19	26.547243134360976	24.53363379246783	25.34553856191348	23.573584511257717
20	26.253112739567126	23.66458028987573	25.395916719123008	24.686390251434137
21	27.203809032119107	24.275374081573023	24.562650992601906	23.95816589370596
22	25.89511109703372	24.138463487639168	24.910045452385855	25.05637996294126
23	24.422282838920143	23.368000981832147	25.746726896262306	26.462989282985404
24	24.43957745910109	25.0063542663851	25.28222048552138	25.27184778899243
25	24.867954292327752	24.174887732412735	25.51956210686527	25.437595868394247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	906.0
1	906.0
2	861.0
3	816.0
4	816.0
5	816.0
6	1649.5
7	2483.0
8	2483.0
9	2483.0
10	2847.5
11	3212.0
12	3212.0
13	3212.0
14	3793.5
15	4375.0
16	4375.0
17	4375.0
18	7651.5
19	10928.0
20	10928.0
21	10928.0
22	16737.5
23	22547.0
24	22547.0
25	22547.0
26	33537.5
27	44528.0
28	44528.0
29	44528.0
30	52429.5
31	60331.0
32	60331.0
33	60331.0
34	72116.5
35	83902.0
36	83902.0
37	83902.0
38	90906.5
39	97911.0
40	97911.0
41	97911.0
42	109672.5
43	121434.0
44	121434.0
45	121434.0
46	137320.0
47	153206.0
48	153206.0
49	153206.0
50	164153.0
51	175100.0
52	175100.0
53	175100.0
54	151893.5
55	128687.0
56	128687.0
57	128687.0
58	119201.0
59	109715.0
60	109715.0
61	109715.0
62	99409.0
63	89103.0
64	89103.0
65	89103.0
66	75939.0
67	62775.0
68	62775.0
69	62775.0
70	49572.5
71	36370.0
72	36370.0
73	36370.0
74	30159.0
75	23948.0
76	23948.0
77	23948.0
78	20532.0
79	17116.0
80	17116.0
81	17116.0
82	12688.5
83	8261.0
84	8261.0
85	8261.0
86	6035.0
87	3809.0
88	3809.0
89	3809.0
90	2581.5
91	1354.0
92	1354.0
93	1354.0
94	734.5
95	115.0
96	115.0
97	115.0
98	69.5
99	24.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18409192402585678
2	0.009263980692914084
3	0.0
4	0.16081320331032908
5	0.053604401103443035
6	0.06294756111851878
7	0.04449877905485227
8	0.027554404112257277
9	0.01908221664095978
10	0.012906229512350391
11	0.01971565121825305
12	0.020269906473384662
13	0.01084756713614726
14	0.011797719002087165
15	0.001504407121071518
16	5.542552551316119E-4
17	0.0
18	0.0
19	0.0
20	7.917932216165884E-5
21	0.004988297296184507
22	0.007601214927519248
23	8.709725437782473E-4
24	0.0019794830540414707
25	0.11568098967818356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1262956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.38475024974036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53685698371169	38.636036043219754
2	11.67494962565991	11.064291443803889
3	3.022154359715542	4.296120886538548
4	1.1342994559948498	2.1499398572292923
5	0.5714879897366502	1.3539907832198674
6	0.34830146087332714	0.9902506641061392
7	0.24636557989123126	0.8171780031294913
8	0.1628692648075572	0.6174015549011939
9	0.13119144932835905	0.5594826655193185
>10	0.8815337417433048	8.13486338366306
>50	0.12520941044190367	4.237588239536234
>100	0.13613782126539273	14.048496398906154
>500	0.02055546196071695	6.670967818949163
>1k	0.007918907476669646	5.8879232820671135
>5k	1.6848739312063076E-4	0.5354689752108386
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6707	0.5310557137382458	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4477	0.3544858253177466	No Hit
GTACGGAAGCAGTGGTATCAACGCA	4065	0.3218639445871432	No Hit
CTTCCGTACTCTGCGTTGATACCAC	2960	0.23437079359851015	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2567	0.20325331998897822	No Hit
TCCGTACTCTGCGTTGATACCACTG	2369	0.18757581420096978	No Hit
GAGTACGGAAGCAGTGGTATCAACG	2344	0.18559633114692833	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1932	0.15297445041632488	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1889	0.14956973956337355	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1810	0.1433145731126025	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1793	0.1419685246358543	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1790	0.1417309866693693	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1632	0.12922065376782724	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1607	0.12724117071378577	No Hit
GCTTCCGTACTCTGCGTTGATACCA	1560	0.12351974257218779	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1551	0.12280712867273286	No Hit
CTTTAATATACGCTATTGGAGCTGG	1551	0.12280712867273286	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1535	0.12154025951814632	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1507	0.11932323849761987	No Hit
CCCATGTACTCTGCGTTGATACCAC	1466	0.11607688628899185	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1465	0.11599770696683019	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1449	0.11473083781224366	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1439	0.11393904459062708	No Hit
GAACTACGACGGTATCTGATCGTCT	1366	0.10815895407282597	No Hit
TCGTAGTTCCGACCATAAACGATGC	1362	0.10784223678417934	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1361	0.10776305746201767	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1360	0.10768387813985601	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1330	0.10530849847500624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.917932216165884E-5	0.0
10	0.0	0.0	0.0	7.917932216165884E-5	0.0
11	0.0	0.0	0.0	7.917932216165884E-5	0.0
12	0.0	0.0	0.0	7.917932216165884E-5	0.0
13	0.0	0.0	0.0	7.917932216165884E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	135	0.0	15.471026	9
GTATTAA	110	1.7644197E-10	13.86761	1
TTAGACC	50	0.0014805953	13.322143	3
AGGCCCG	330	0.0	12.945798	10
CTCGTAG	165	0.0	12.6581135	10
TCTAGCG	150	1.8189894E-12	12.65711	17
CGCCAGT	250	0.0	12.53054	18
CATCGCC	260	0.0	12.4122305	15
TGTACCG	115	5.316906E-9	12.391769	5
GTTCAAA	410	0.0	12.324415	1
TCGCCAG	255	0.0	12.2848425	17
ATCGCCA	255	0.0	12.2848425	16
CGCGCCA	70	1.0974864E-4	12.2060375	10
TAAGGGT	55	0.0030299043	12.11104	4
CGAACGA	165	0.0	12.081788	16
AATCGGT	55	0.0030852861	12.081788	14
CCGCATC	260	0.0	12.049551	12
GTCATAG	80	2.7625234E-5	11.917478	1
ATTCGCA	80	2.8875755E-5	11.866042	14
AAGCTCG	185	0.0	11.805173	7
>>END_MODULE