FastQCFastQC Report
Thu 2 Feb 2017
SRR4050249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5938651
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA120030.2021166086372141No Hit
GTATCTGATCGTCTTCGAACCTCCG80170.13499698837328544No Hit
GAATAGGACCGCGGTTCTATTTTGT75180.12659440670953725No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG70060.11797292011266532No Hit
GTCTTGCGCCGGTCCAAGAATTTCA68380.11514399482306671No Hit
GTCCTATTCCATTATTCCTAGCTGC67050.11290442896880117No Hit
GATTAAGAGGGACGGCCGGGGGCAT65620.11049647470444046No Hit
CTATTGGAGCTGGAATTACCGCGGC64920.10931775583377437No Hit
GACTTAATCAACGCAAGCTTATGAC64270.10822323116815587No Hit
GAACTACGACGGTATCTGATCGTCT63530.10697715693345172No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC62960.10601734299590934No Hit
CTTATGACCCGCACTTACTGGGAAT62240.10480494644322423No Hit
GGGTAGGCACACGCTGAGCCAGTCA62220.10477126876120521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.015.7165691
GTATCAA91150.013.5734011
CTCGACG3600.012.41423713
CCGTCCA4300.012.1641439
ACGCTTA3550.012.05307217
CGCTTAC3700.011.821321518
AACCGCG5150.011.8179357
CGTCGTA9600.011.78862110
TAGATCG1950.011.7040025
ACCGTCC5450.011.5168768
CGGTTCT13450.011.38186712
CGAACGA4950.011.33359716
GCGTTAT4400.011.1871761
ATCGCCA9400.011.12719716
CGACCAT9450.011.07001310
CGGAATT3700.011.05064715
GTCGTAG10250.011.0410511
CGGTAGC3650.010.94318410
CGAGCCG14800.010.92215115
GTTTTCG7000.010.86708115
Produced by FastQC (version 0.11.5)