##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050249_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5938651
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.335657710816818	31.0	31.0	34.0	27.0	34.0
2	30.451705277848454	31.0	31.0	34.0	27.0	34.0
3	30.556238950563014	31.0	31.0	34.0	27.0	34.0
4	34.0963843472196	37.0	35.0	37.0	30.0	37.0
5	34.12015826489888	37.0	35.0	37.0	30.0	37.0
6	34.155563275228666	37.0	35.0	37.0	30.0	37.0
7	34.079379980402955	37.0	35.0	37.0	30.0	37.0
8	34.05218777799874	37.0	35.0	37.0	30.0	37.0
9	35.611993868641214	39.0	37.0	39.0	30.0	39.0
10	35.48754557221834	39.0	37.0	39.0	30.0	39.0
11	35.40285748396395	39.0	37.0	39.0	30.0	39.0
12	35.321908965520954	39.0	37.0	39.0	30.0	39.0
13	35.32627207761493	39.0	37.0	39.0	30.0	39.0
14	36.4763792315797	40.0	37.0	41.0	30.0	41.0
15	36.42831680123988	40.0	37.0	41.0	30.0	41.0
16	36.430882367056086	40.0	37.0	41.0	30.0	41.0
17	36.386845598436416	40.0	37.0	41.0	30.0	41.0
18	36.34149354794548	40.0	37.0	41.0	30.0	41.0
19	36.3585691430596	40.0	37.0	41.0	30.0	41.0
20	36.31254000277168	40.0	37.0	41.0	29.0	41.0
21	36.258642745633644	40.0	37.0	41.0	29.0	41.0
22	36.17409761913943	40.0	37.0	41.0	29.0	41.0
23	36.08969848539677	40.0	37.0	41.0	29.0	41.0
24	36.023369785495056	40.0	37.0	41.0	29.0	41.0
25	35.77685403637964	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.671615828030486
1101	2	0.6576348344198095
1101	3	0.65092084208624
1101	4	0.7089038703188137
1101	5	0.7040993638534374
1101	6	0.6874229429982179
1101	7	0.66618735286076
1101	8	0.7508090550834652
1101	9	0.707735772622641
1101	10	0.7607413403027508
1101	11	0.6455761343053013
1101	12	0.6665971086695848
1101	13	0.6343877444553314
1101	14	0.7698607618521436
1101	15	0.79001505751868
1101	16	0.8482038613826788
1101	17	0.826610198416553
1101	18	0.822301493286659
1101	19	0.8317325964900775
1101	20	0.8445313131064651
1101	21	0.8662843524557218
1101	22	0.8941848687056293
1101	23	0.8594707447831738
1101	24	0.8966496888594548
1101	25	0.9458681061994412
1102	1	0.444258003938387
1102	2	0.45321924105436295
1102	3	0.42030139205910544
1102	4	0.36957422104050863
1102	5	0.3238672422597375
1102	6	0.3788149408232613
1102	7	0.4176994833678265
1102	8	0.39994593186091976
1102	9	0.5110115126592589
1102	10	0.5717091947027768
1102	11	0.4841691477019623
1102	12	0.44871951645769315
1102	13	0.47404833202414665
1102	14	0.5892050894174403
1102	15	0.5512246218245949
1102	16	0.5888052517968134
1102	17	0.5421571889559402
1102	18	0.6237936359161438
1102	19	0.6408081105306778
1102	20	0.6016754529191815
1102	21	0.6377060987553591
1102	22	0.6676274590058213
1102	23	0.6527002689467309
1102	24	0.6470945765113001
1102	25	0.6900049559982335
1103	1	0.4784784828835882
1103	2	0.4573159043708941
1103	3	0.4267174315643416
1103	4	0.42468830469142915
1103	5	0.4393842166001036
1103	6	0.3792842755780512
1103	7	0.40931968996838464
1103	8	0.4555045021627251
1103	9	0.48316180492006566
1103	10	0.4292901086080363
1103	11	0.5141057664303901
1103	12	0.3782340659999548
1103	13	0.44503241209128674
1103	14	0.5897533257343568
1103	15	0.5571797584434748
1103	16	0.6114216897761295
1103	17	0.4214374691615319
1103	18	0.51768156291255
1103	19	0.5123713469060007
1103	20	0.5537093797429975
1103	21	0.46686535103045657
1103	22	0.5809428583673721
1103	23	0.6608735522277414
1103	24	0.6874431395890213
1103	25	0.6100049428087146
1104	1	0.43056286516012676
1104	2	0.481333691365915
1104	3	0.4978857972801656
1104	4	0.4858321632726472
1104	5	0.5082241364184696
1104	6	0.5037396533261145
1104	7	0.5218961615105044
1104	8	0.47315818162589807
1104	9	0.5840624291734002
1104	10	0.5247147755179071
1104	11	0.44121285025703116
1104	12	0.48301610754374735
1104	13	0.5287294240107343
1104	14	0.6027412495821451
1104	15	0.47951714766998776
1104	16	0.6247379910145625
1104	17	0.5325338675890308
1104	18	0.5952991958643139
1104	19	0.6704946466010426
1104	20	0.6156737715457723
1104	21	0.6124636563696839
1104	22	0.6480322487602592
1104	23	0.6396174982499119
1104	24	0.5571589620378461
1104	25	0.6518916602708913
1105	1	0.386114156025279
1105	2	0.42125162539825567
1105	3	0.4645318883729246
1105	4	0.46724019456511456
1105	5	0.501967199768302
1105	6	0.45148498827308714
1105	7	0.4581504081923953
1105	8	0.49617030366652415
1105	9	0.5319672684032142
1105	10	0.5206944198178931
1105	11	0.4343323450703451
1105	12	0.44950145232146355
1105	13	0.5190899208960857
1105	14	0.6537932286413906
1105	15	0.6144407243458048
1105	16	0.5186516674288697
1105	17	0.5513801794239654
1105	18	0.6874283477515064
1105	19	0.6176815570309984
1105	20	0.6136978692267547
1105	21	0.5571314520094433
1105	22	0.652668960094168
1105	23	0.6275141450808732
1105	24	0.6242328716939483
1105	25	0.6565364846959056
1106	1	0.2563007386055922
1106	2	0.30322170376910407
1106	3	0.3284893811025107
1106	4	0.3909099828466509
1106	5	0.3398731461742628
1106	6	0.37716316859575016
1106	7	0.4101545115494645
1106	8	0.3413702102127303
1106	9	0.33124738641176776
1106	10	0.3320221994432728
1106	11	0.35469579942090235
1106	12	0.4160290697074842
1106	13	0.3250088813051093
1106	14	0.3773384508869171
1106	15	0.43125112975720725
1106	16	0.31735559659635726
1106	17	0.38540396330959226
1106	18	0.4109943745334519
1106	19	0.3868616875143971
1106	20	0.3932329848806688
1106	21	0.42402262078881847
1106	22	0.43069123245366825
1106	23	0.2927734096263279
1106	24	0.4405205743857792
1106	25	0.44495794060794935
1107	1	0.2184613778861717
1107	2	0.1875182916335767
1107	3	0.15348642441830052
1107	4	0.15758585917259182
1107	5	0.16791912180444513
1107	6	0.10060711125998267
1107	7	0.22751196844007637
1107	8	0.22770764187031034
1107	9	0.2670508987707976
1107	10	0.21657162981538391
1107	11	0.1743007021554135
1107	12	0.20990600840875828
1107	13	0.2427531948818782
1107	14	0.22910897370096706
1107	15	0.269125103747875
1107	16	0.21301779164492274
1107	17	0.27289112052434916
1107	18	0.2641390293605852
1107	19	0.31301683088774723
1107	20	0.2725684961014565
1107	21	0.29120979617161424
1107	22	0.24872822783459014
1107	23	0.14565135313461042
1107	24	0.17693433882724463
1107	25	0.26751974927653066
1108	1	0.2252303652413019
1108	2	0.15270633782034793
1108	3	0.15959217869950493
1108	4	0.20756018988934244
1108	5	0.25350863769178744
1108	6	0.23059775986710207
1108	7	0.2003142240305138
1108	8	0.1588272113087541
1108	9	0.09508420077452939
1108	10	0.0617162923632435
1108	11	0.03744323447813258
1108	12	0.044476755380500776
1108	13	0.09347294610704182
1108	14	-0.027745138238564948
1108	15	0.13282565058539575
1108	16	0.11115958704200324
1108	17	0.0686509928656136
1108	18	0.006743347428241009
1108	19	0.08490044447560052
1108	20	0.1377010794004363
1108	21	0.14841378949953565
1108	22	0.18418778802892888
1108	23	0.09941181958919287
1108	24	0.02922691624488749
1108	25	0.007812952465400258
1109	1	-0.08977149357468761
1109	2	-0.11096606008002396
1109	3	-0.016130089222464505
1109	4	-0.04989202362757794
1109	5	-0.019760287898698436
1109	6	-0.09493749070545476
1109	7	-0.1275879391319208
1109	8	-0.07195107945445756
1109	9	-0.051100839502126405
1109	10	-0.21260229205127956
1109	11	-0.16771074900614735
1109	12	-0.14496832562364403
1109	13	-0.13531023861349212
1109	14	-0.16302931513681074
1109	15	-0.07240571131637097
1109	16	-0.12809721081453063
1109	17	-0.16534173174247258
1109	18	-0.13337171362831413
1109	19	-0.1876398668622059
1109	20	-0.18901721564915164
1109	21	-0.13461803821596874
1109	22	-0.1278912907196741
1109	23	-0.13547871140794854
1109	24	-0.14192365270539398
1109	25	-0.1861076355169402
1110	1	-0.11344975318216655
1110	2	-0.1495392769315842
1110	3	-0.06752849526934313
1110	4	-0.07499601607806738
1110	5	-0.03845825283872273
1110	6	-0.025161150054422876
1110	7	-0.011954903422264351
1110	8	-0.01926381023186252
1110	9	-0.06407581827854614
1110	10	-0.24095696052607707
1110	11	-0.1897905174538721
1110	12	-0.22325415431227214
1110	13	-0.2511659339321852
1110	14	-0.25515628176516714
1110	15	-0.24393125831402074
1110	16	-0.16520320325910376
1110	17	-0.212420708497298
1110	18	-0.27472049863576586
1110	19	-0.2857650802389742
1110	20	-0.24925944196412786
1110	21	-0.2598466917651123
1110	22	-0.2791765500321972
1110	23	-0.26490723683783557
1110	24	-0.27402176871628114
1110	25	-0.26322130877527883
1111	1	-0.0697710643458862
1111	2	-0.16083172673406665
1111	3	-0.18150048420075748
1111	4	-0.12246008724878976
1111	5	-0.13197443880036275
1111	6	-0.13340387740353776
1111	7	-0.16300306891276506
1111	8	-0.19795107758824315
1111	9	-0.15104956532243108
1111	10	-0.12284743216967087
1111	11	-0.271770061640332
1111	12	-0.29221783212716446
1111	13	-0.30868344979079865
1111	14	-0.31315884298640384
1111	15	-0.2838688432979879
1111	16	-0.2698297911764911
1111	17	-0.2650932434697708
1111	18	-0.18688437242123257
1111	19	-0.18631569388792002
1111	20	-0.22640464541223793
1111	21	-0.14276055524165798
1111	22	-0.09709396513745361
1111	23	-0.09958466492594908
1111	24	-0.21123539874241715
1111	25	-0.1624322315700084
1112	1	-0.09671142387918508
1112	2	-0.10920284124161839
1112	3	-0.13030650558633639
1112	4	-0.19289710391532822
1112	5	-0.06453892236305592
1112	6	-0.11815959281352661
1112	7	-0.08327693012910231
1112	8	-0.05577302160563136
1112	9	-0.0875881749522236
1112	10	-0.16261542460693335
1112	11	-0.13590592176939964
1112	12	-0.20844285167697763
1112	13	-0.15540038676292056
1112	14	-0.15431720813741379
1112	15	-0.2144032440184631
1112	16	-0.10250520636061822
1112	17	-0.1312793133984087
1112	18	-0.14741805515521378
1112	19	-0.2591751961411788
1112	20	-0.2110207534954185
1112	21	-0.2443373294162754
1112	22	-0.1986180466877201
1112	23	-0.17945954734160097
1112	24	-0.1266609863808128
1112	25	-0.09493845946734325
1113	1	-0.3153864585558921
1113	2	-0.28854818726666664
1113	3	-0.17915651565978763
1113	4	-0.13560214420497374
1113	5	-0.1684009915572915
1113	6	-0.20785733544892082
1113	7	-0.19743110032976574
1113	8	-0.22161660871526578
1113	9	-0.3064816749659016
1113	10	-0.41723167594073374
1113	11	-0.27435244253272373
1113	12	-0.4210372332625454
1113	13	-0.36116494911507857
1113	14	-0.3935644851970608
1113	15	-0.29612148840282515
1113	16	-0.30136765279100786
1113	17	-0.33656393600886503
1113	18	-0.24681269475373568
1113	19	-0.3485648280333038
1113	20	-0.2641973306949339
1113	21	-0.32862005423866236
1113	22	-0.3237376637142546
1113	23	-0.3036996030233112
1113	24	-0.32196563981685955
1113	25	-0.30936670624777207
1114	1	-0.3746625173257563
1114	2	-0.34818588771501524
1114	3	-0.39192044929295733
1114	4	-0.37805526524221733
1114	5	-0.34800807576180404
1114	6	-0.37132959839537705
1114	7	-0.4205216837029866
1114	8	-0.3801661366650819
1114	9	-0.4242246399394958
1114	10	-0.45850841919200747
1114	11	-0.6267441728994285
1114	12	-0.5805445114034669
1114	13	-0.5337350217734027
1114	14	-0.7536369999999906
1114	15	-0.8289972541886641
1114	16	-0.7157135409213566
1114	17	-0.6379615498358504
1114	18	-0.7016483296479592
1114	19	-0.5761610254845095
1114	20	-0.7980928208381997
1114	21	-0.7280420058110266
1114	22	-0.8385682304709334
1114	23	-0.7890159158788776
1114	24	-0.7209009642850361
1114	25	-0.7699747591391031
1115	1	-0.430813450651538
1115	2	-0.5067768029788482
1115	3	-0.44533102853766593
1115	4	-0.30154849225014857
1115	5	-0.2976743977084837
1115	6	-0.3734496913076839
1115	7	-0.46322288364817155
1115	8	-0.44612688536653167
1115	9	-0.33782167209666625
1115	10	-0.4181548077468591
1115	11	-0.5722044139818294
1115	12	-0.5626937221095858
1115	13	-0.49125433000261154
1115	14	-0.5981787028547316
1115	15	-0.5204925599577592
1115	16	-0.5718309483416064
1115	17	-0.6115189550383633
1115	18	-0.5277229487934676
1115	19	-0.5611497705056294
1115	20	-0.5914285816422407
1115	21	-0.6684149198430234
1115	22	-0.7946046112534546
1115	23	-0.6335475764642169
1115	24	-0.5615214402056239
1115	25	-0.6408697136498986
1116	1	0.14776481906057626
1116	2	-0.026903335004636375
1116	3	0.10020370538587287
1116	4	0.0811013220616914
1116	5	0.00866906504313647
1116	6	0.045010211050069415
1116	7	0.022745416252071493
1116	8	0.0023002035171728608
1116	9	0.0027121923130479786
1116	10	0.12146946312986273
1116	11	-0.047572880447546595
1116	12	-0.05928237453833418
1116	13	-0.13605013967862334
1116	14	-0.01700778364945421
1116	15	-0.06400843537419121
1116	16	0.009695185299776199
1116	17	-0.09328780890457011
1116	18	0.04308480888931143
1116	19	-0.0969569390562981
1116	20	0.04789573588580964
1116	21	-0.04237210230536448
1116	22	-0.04004861497652712
1116	23	0.024761392395951987
1116	24	-0.11679753824844141
1116	25	-0.080212462557391
1201	1	0.5226183135098417
1201	2	0.564672273615578
1201	3	0.569868522472202
1201	4	0.6489107562911443
1201	5	0.6455276020767542
1201	6	0.678395459684296
1201	7	0.6679899434129908
1201	8	0.6870947042377935
1201	9	0.8171983893789374
1201	10	0.7414662318378404
1201	11	0.6743487989010788
1201	12	0.6873868385503812
1201	13	0.7017687953690626
1201	14	0.7806232780834321
1201	15	0.883797273594034
1201	16	0.7121187244208969
1201	17	0.7677030544985968
1201	18	0.7693646609962244
1201	19	0.7834562974633812
1201	20	0.7998680265860401
1201	21	0.8151518090938197
1201	22	0.829111156343771
1201	23	0.7739159824322499
1201	24	0.800569097884889
1201	25	0.7074939165372314
1202	1	0.333763836944577
1202	2	0.33179091951473083
1202	3	0.35474323977030764
1202	4	0.42073968545980733
1202	5	0.4594502940456664
1202	6	0.3821727442671019
1202	7	0.41065997113977204
1202	8	0.3912441302461289
1202	9	0.3397190497531568
1202	10	0.47095288294809734
1202	11	0.31074182374713644
1202	12	0.2706436246938111
1202	13	0.26831079044843165
1202	14	0.3352972663379674
1202	15	0.3713341492029585
1202	16	0.29707701918244567
1202	17	0.299907881588247
1202	18	0.33607999231084307
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2210	10	-0.24145123848155947
2210	11	-0.17424758904520132
2210	12	-0.09753354197703601
2210	13	-0.044095450990461416
2210	14	-0.13786127684171845
2210	15	-0.08111368128689378
2210	16	-0.03571594222884045
2210	17	-0.05878346172269033
2210	18	-0.0577211401559552
2210	19	-0.012498952402204111
2210	20	-0.051494500333518545
2210	21	-0.042755247790381645
2210	22	0.007004828492597426
2210	23	-0.10147235605424498
2210	24	0.02256469290262686
2210	25	-0.061751367928728484
2211	1	-0.2815190025823533
2211	2	-0.33836484108346454
2211	3	-0.3110089003621397
2211	4	-0.39748580761644803
2211	5	-0.3450812557583305
2211	6	-0.39941541486874854
2211	7	-0.3152439824070896
2211	8	-0.341740929029541
2211	9	-0.24652801801960322
2211	10	-0.33629903726426846
2211	11	-0.2264229520995329
2211	12	-0.2681223910684096
2211	13	-0.17978798917906857
2211	14	-0.2550118642465904
2211	15	-0.21722583147506214
2211	16	-0.3041520049004305
2211	17	-0.3974473367186988
2211	18	-0.3267757311364363
2211	19	-0.3084157369616065
2211	20	-0.4017766212090166
2211	21	-0.45542145760933295
2211	22	-0.4308293345648906
2211	23	-0.3585125791190933
2211	24	-0.5204618639519794
2211	25	-0.5048776150884748
2212	1	-0.37412303442139105
2212	2	-0.2799903715008689
2212	3	-0.41333577392577325
2212	4	-0.30964352943036033
2212	5	-0.3258877344751596
2212	6	-0.3742580646203919
2212	7	-0.3935882362090197
2212	8	-0.4041802366255425
2212	9	-0.3995159565159483
2212	10	-0.4578550095709346
2212	11	-0.29635705670590085
2212	12	-0.2863528249776479
2212	13	-0.30489517571223246
2212	14	-0.25227750245997527
2212	15	-0.3041869661848864
2212	16	-0.3289369643164122
2212	17	-0.352859822241264
2212	18	-0.4472440094127208
2212	19	-0.46725146252434513
2212	20	-0.44565686383582204
2212	21	-0.37849576318235734
2212	22	-0.35605049578037296
2212	23	-0.36874064090245895
2212	24	-0.5130339948364124
2212	25	-0.4739093937914802
2213	1	-0.5523091845808565
2213	2	-0.5688874669256307
2213	3	-0.6198731023962125
2213	4	-0.5979757898314233
2213	5	-0.6228902050114016
2213	6	-0.49670555276230033
2213	7	-0.5655740543577679
2213	8	-0.6229924786631074
2213	9	-0.6125520337638761
2213	10	-0.5730301508071136
2213	11	-0.49593522281337243
2213	12	-0.5918749062429001
2213	13	-0.5458038097458058
2213	14	-0.7590322991452894
2213	15	-0.7104395986581196
2213	16	-0.6611213626845966
2213	17	-0.7059266336141832
2213	18	-0.621078890720085
2213	19	-0.627137366746993
2213	20	-0.6506579347737969
2213	21	-0.5873346709771852
2213	22	-0.6086975573020723
2213	23	-0.6049970565588154
2213	24	-0.6457299505598044
2213	25	-0.5745376076025508
2214	1	-0.3674768460011535
2214	2	-0.3632956542740864
2214	3	-0.41547257297684936
2214	4	-0.604850410222916
2214	5	-0.4852188730458593
2214	6	-0.4743251204748873
2214	7	-0.5038217336645019
2214	8	-0.46256642640028645
2214	9	-0.42381220846748846
2214	10	-0.48682210501975476
2214	11	-0.3459665479878069
2214	12	-0.38210524790250844
2214	13	-0.43639334474630687
2214	14	-0.5114880672816113
2214	15	-0.3912776487018732
2214	16	-0.5180442860168313
2214	17	-0.5256511514217124
2214	18	-0.518786025447028
2214	19	-0.5719322765257076
2214	20	-0.563976544079523
2214	21	-0.621562146742022
2214	22	-0.5509584158987977
2214	23	-0.4713894486065229
2214	24	-0.5744228538534912
2214	25	-0.5593352433888157
2215	1	-0.38830324050169196
2215	2	-0.30263547449686
2215	3	-0.46333046442051895
2215	4	-0.4134113015535448
2215	5	-0.5175064964373206
2215	6	-0.4376860767710653
2215	7	-0.3997541699186371
2215	8	-0.49202473940562186
2215	9	-0.6177950270560331
2215	10	-0.5004613289966571
2215	11	-0.38968729826000725
2215	12	-0.4520657643155559
2215	13	-0.41714272187969215
2215	14	-0.5104066599077299
2215	15	-0.501624346615877
2215	16	-0.5441267754494277
2215	17	-0.5683934264680062
2215	18	-0.5159579336901317
2215	19	-0.6242288358311399
2215	20	-0.6219457909204422
2215	21	-0.47068377770495573
2215	22	-0.6278394016269715
2215	23	-0.5205861513320116
2215	24	-0.5923644062946778
2215	25	-0.5408859007552422
2216	1	0.3132994276740959
2216	2	0.24127183515440365
2216	3	0.21273829027615676
2216	4	0.36245033553372963
2216	5	0.37102851177316865
2216	6	0.49097988172191975
2216	7	0.4427434411548532
2216	8	0.4096134147659569
2216	9	0.5241760924459555
2216	10	0.6064904471586061
2216	11	0.6231194352656786
2216	12	0.4993731580234595
2216	13	0.5400055028927753
2216	14	0.6437932120643666
2216	15	0.7208605544408186
2216	16	0.5985955802640532
2216	17	0.4887276619547407
2216	18	0.6196966785615814
2216	19	0.5096343134698103
2216	20	0.6790148822465838
2216	21	0.6193414103940142
2216	22	0.6454820875787917
2216	23	0.6167764274097891
2216	24	0.4520879496971233
2216	25	0.6865345688659659
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181440.0
3	11.0
4	1592.0
5	8495.0
6	10555.0
7	10442.0
8	8862.0
9	8567.0
10	8745.0
11	10103.0
12	16544.0
13	13502.0
14	6831.0
15	6820.0
16	7185.0
17	7815.0
18	8644.0
19	10460.0
20	12761.0
21	15993.0
22	19644.0
23	23319.0
24	27446.0
25	31425.0
26	36634.0
27	41766.0
28	48530.0
29	59021.0
30	74836.0
31	96790.0
32	126521.0
33	169803.0
34	237348.0
35	339732.0
36	519392.0
37	980535.0
38	2750542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.033539720806125	16.695211528470697	11.9698045555205	26.301444195202677
2	17.49836828107054	19.586732396409037	37.24562471645194	25.669274606068477
3	19.478460757881212	22.95391031298157	27.47226630286641	30.095362626270806
4	13.263056597668191	15.577286160427404	35.78490412798075	35.37475311392366
5	16.146638404902905	34.639228126584136	32.63415551673734	16.579977951775625
6	34.40746974678747	35.42115899161182	15.760332649272687	14.41103861232802
7	30.639264736630086	29.680036567056927	20.331041040725665	19.34965765558732
8	27.48759133901663	32.277935135461185	19.157803257160833	21.076670268361354
9	27.519920414387084	14.598109673193827	17.843963199067996	40.0380067133511
10	16.409532983943137	25.549996368826772	30.276874009747047	27.76359663748304
11	37.864361617354945	21.09265590211396	21.538696032840274	19.504286447690824
12	25.63197305750904	22.938469652413122	27.78732488126567	23.64223240881217
13	30.169959332866487	19.34715658846203	24.080108626462312	26.40277545220917
14	24.11721110618312	19.626447305692555	24.138107523506775	32.11823406461755
15	25.601032588358436	26.375541647911234	21.911962894067496	26.111462869662837
16	26.060190869270755	25.66198159915555	22.87472009252624	25.403107439047456
17	25.648164389365537	24.82665819298803	23.809698125198604	25.715479292447835
18	26.93871255604887	23.66687372933999	24.652486404626455	24.74192730998469
19	27.46776438677211	24.116148712194054	23.716655743361827	24.699431157672016
20	26.621477184859803	24.083399407921966	23.74398676044908	25.551136646769145
21	27.057847763370873	24.014535365044114	23.548772565972893	25.37884430561212
22	26.21444950435648	23.938505968229325	24.331241388310303	25.515803139103895
23	25.13482663597503	23.401254168162104	24.48537453387247	26.978544661990394
24	25.504660635063853	24.82809660823177	24.018175476867313	25.649067279837062
25	25.504247022068515	24.049410649854366	24.168016138073803	26.278326190003316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2242.0
1	2242.0
2	2288.5
3	2335.0
4	2335.0
5	2335.0
6	3883.5
7	5432.0
8	5432.0
9	5432.0
10	7953.5
11	10475.0
12	10475.0
13	10475.0
14	15464.0
15	20453.0
16	20453.0
17	20453.0
18	32468.5
19	44484.0
20	44484.0
21	44484.0
22	66666.5
23	88849.0
24	88849.0
25	88849.0
26	127198.5
27	165548.0
28	165548.0
29	165548.0
30	205309.5
31	245071.0
32	245071.0
33	245071.0
34	306445.5
35	367820.0
36	367820.0
37	367820.0
38	416940.5
39	466061.0
40	466061.0
41	466061.0
42	528278.0
43	590495.0
44	590495.0
45	590495.0
46	637541.0
47	684587.0
48	684587.0
49	684587.0
50	705840.5
51	727094.0
52	727094.0
53	727094.0
54	686747.5
55	646401.0
56	646401.0
57	646401.0
58	610793.5
59	575186.0
60	575186.0
61	575186.0
62	517585.5
63	459985.0
64	459985.0
65	459985.0
66	395828.5
67	331672.0
68	331672.0
69	331672.0
70	265187.5
71	198703.0
72	198703.0
73	198703.0
74	165409.0
75	132115.0
76	132115.0
77	132115.0
78	111733.0
79	91351.0
80	91351.0
81	91351.0
82	70320.0
83	49289.0
84	49289.0
85	49289.0
86	36161.0
87	23033.0
88	23033.0
89	23033.0
90	16062.5
91	9092.0
92	9092.0
93	9092.0
94	4909.5
95	727.0
96	727.0
97	727.0
98	439.0
99	151.0
100	151.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13841527309821708
2	0.15695483704969362
3	0.170459587539325
4	0.19064935790973403
5	0.180781797078158
6	0.06722065330998572
7	0.054793588644963316
8	0.05863284439513284
9	0.06583986834720545
10	0.06617664516739576
11	0.682427709592633
12	0.6412735821653773
13	0.640936805345187
14	0.6418629416007103
15	0.6422165572619102
16	0.6480764739332215
17	0.6403137682278349
18	0.6510906264739248
19	0.646981949267603
20	0.6525556056417526
21	0.6400106690896636
22	0.6439341190448807
23	0.6292506496845832
24	0.6390845328341402
25	0.6327194509325434
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5938651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.7700939407057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.39188735913812	41.1580027433862
2	9.907190774647063	9.663492495360604
3	2.334038926284798	3.414938930885204
4	0.9158967814695107	1.7867348828903216
5	0.49447003015855323	1.205767491084811
6	0.3324338665450095	0.9727698540283279
7	0.22979704292327066	0.7845056359465005
8	0.16609953678933545	0.6480552010178874
9	0.13039065064079097	0.5723247852667002
>10	0.871051113762445	8.284023503692312
>50	0.1065369285534409	3.657986371335971
>100	0.09399115236629844	9.483955754027072
>500	0.013403067684920352	4.57342914492073
>1k	0.01204886410752429	11.387378679584998
>5k	7.291819776880333E-4	2.2033707316003506
>10k+	3.472295131847777E-5	0.20326379497201644
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	12003	0.2021166086372141	No Hit
GTATCTGATCGTCTTCGAACCTCCG	8017	0.13499698837328544	No Hit
GAATAGGACCGCGGTTCTATTTTGT	7518	0.12659440670953725	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	7006	0.11797292011266532	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	6838	0.11514399482306671	No Hit
GTCCTATTCCATTATTCCTAGCTGC	6705	0.11290442896880117	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	6562	0.11049647470444046	No Hit
CTATTGGAGCTGGAATTACCGCGGC	6492	0.10931775583377437	No Hit
GACTTAATCAACGCAAGCTTATGAC	6427	0.10822323116815587	No Hit
GAACTACGACGGTATCTGATCGTCT	6353	0.10697715693345172	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	6296	0.10601734299590934	No Hit
CTTATGACCCGCACTTACTGGGAAT	6224	0.10480494644322423	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	6222	0.10477126876120521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.683884100951546E-5
3	0.0	0.0	0.0	0.0	1.683884100951546E-5
4	0.0	0.0	0.0	0.0	1.683884100951546E-5
5	0.0	0.0	0.0	0.0	1.683884100951546E-5
6	0.0	0.0	0.0	0.0	1.683884100951546E-5
7	0.0	0.0	0.0	1.683884100951546E-5	1.683884100951546E-5
8	0.0	0.0	0.0	1.683884100951546E-5	1.683884100951546E-5
9	0.0	0.0	0.0	1.683884100951546E-5	1.683884100951546E-5
10	0.0	0.0	0.0	5.051652302854638E-5	1.683884100951546E-5
11	0.0	0.0	0.0	5.051652302854638E-5	1.683884100951546E-5
12	0.0	0.0	0.0	6.735536403806184E-5	1.0103304605709276E-4
13	0.0	0.0	0.0	6.735536403806184E-5	1.1787188706660822E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2620	0.0	15.716569	1
GTATCAA	9115	0.0	13.573401	1
CTCGACG	360	0.0	12.414237	13
CCGTCCA	430	0.0	12.164143	9
ACGCTTA	355	0.0	12.053072	17
CGCTTAC	370	0.0	11.8213215	18
AACCGCG	515	0.0	11.817935	7
CGTCGTA	960	0.0	11.788621	10
TAGATCG	195	0.0	11.704002	5
ACCGTCC	545	0.0	11.516876	8
CGGTTCT	1345	0.0	11.381867	12
CGAACGA	495	0.0	11.333597	16
GCGTTAT	440	0.0	11.187176	1
ATCGCCA	940	0.0	11.127197	16
CGACCAT	945	0.0	11.070013	10
CGGAATT	370	0.0	11.050647	15
GTCGTAG	1025	0.0	11.04105	11
CGGTAGC	365	0.0	10.943184	10
CGAGCCG	1480	0.0	10.922151	15
GTTTTCG	700	0.0	10.867081	15
>>END_MODULE