FastQCFastQC Report
Thu 2 Feb 2017
SRR4050249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5938651
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA118400.19937187755266306No Hit
GAATAGGACCGCGGTTCTATTTTGT77460.13043366245970675No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG70330.11842756881992222No Hit
GATTAAGAGGGACGGCCGGGGGCAT69010.1162048418066662No Hit
GTATCTGATCGTCTTCGAACCTCCG67130.11303913969687729No Hit
CTTATGACCCGCACTTACTGGGAAT66690.1122982306924586No Hit
GAACTACGACGGTATCTGATCGTCT64080.10790329318897507No Hit
GCGCAAGACGGACCAGAGCGAAAGC63810.10744864448171815No Hit
ATACAGGACTCTTTCGAGGCCCTGT63220.10645515286215675No Hit
GGGTAGGCACACGCTGAGCCAGTCA62270.10485546296625278No Hit
ATCAGATACCGTCGTAGTTCCGACC61880.10419874816688167No Hit
CTATTGGAGCTGGAATTACCGCGGC61300.10322209538832978No Hit
GTCCTATTCCATTATTCCTAGCTGC60210.10138666171829258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.016.5861571
GTATCAA88600.013.3760371
CTCGACG3500.013.28924913
ACGCTTA3600.012.91966917
TCGACGC3550.012.83447114
GACGCTT3900.012.16943616
AACCGCG6250.011.8511077
GCGTTAT4100.011.8407221
CGCTTAC4050.011.4841518
ACGGTAT12000.011.4733669
CGTTATT4250.011.1972282
CGACCAT11400.011.16083510
ACCGTCC3450.010.7337048
CGGTTCT14900.010.64146312
CGACGCT4400.010.57081515
CCGACCA12850.010.5666429
TCCGCGA1355.7423676E-810.55004310
CGTCGTA10250.010.46770210
TCGCGTA6950.010.3832399
AGAACCG7900.010.3428185
Produced by FastQC (version 0.11.5)