##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5938651
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.646187324360366	31.0	31.0	34.0	27.0	34.0
2	30.745126628926332	31.0	31.0	34.0	27.0	34.0
3	30.85709751254957	31.0	31.0	34.0	27.0	34.0
4	34.40064031376823	37.0	35.0	37.0	30.0	37.0
5	34.26408606937838	37.0	35.0	37.0	30.0	37.0
6	34.300133313104276	37.0	35.0	37.0	30.0	37.0
7	34.23680916760389	37.0	35.0	37.0	30.0	37.0
8	34.18424504150858	37.0	35.0	37.0	30.0	37.0
9	35.8007960056922	39.0	37.0	39.0	30.0	39.0
10	35.6498809241358	39.0	35.0	39.0	30.0	39.0
11	35.65025272574529	39.0	35.0	39.0	30.0	39.0
12	35.52767387745129	39.0	35.0	39.0	30.0	39.0
13	35.549722992645975	39.0	35.0	39.0	30.0	39.0
14	36.6790944610148	40.0	37.0	41.0	31.0	41.0
15	36.64009216908015	40.0	37.0	41.0	30.0	41.0
16	36.59981298783175	40.0	37.0	41.0	30.0	41.0
17	36.55927533037385	40.0	37.0	41.0	30.0	41.0
18	36.50494699890598	40.0	36.0	41.0	30.0	41.0
19	36.50462083055562	40.0	37.0	41.0	30.0	41.0
20	36.45597695503575	40.0	37.0	41.0	30.0	41.0
21	36.397272545566324	40.0	37.0	41.0	30.0	41.0
22	36.33462599502816	40.0	36.0	41.0	30.0	41.0
23	36.247171958749554	40.0	36.0	41.0	30.0	41.0
24	36.16166668154098	40.0	36.0	41.0	30.0	41.0
25	35.8381943980207	39.0	36.0	41.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7163112156145672
1101	2	0.6900142424268765
1101	3	0.6819202136071674
1101	4	0.6317770715173765
1101	5	0.724235673601747
1101	6	0.7646837944635791
1101	7	0.8212422893830791
1101	8	0.8276499914111994
1101	9	0.9444104921591361
1101	10	0.9760535822381087
1101	11	0.9218857947874142
1101	12	0.973693896712696
1101	13	1.002057933528576
1101	14	1.0563654881565228
1101	15	1.0777000553385676
1101	16	1.1277413633683722
1101	17	1.1196410188087853
1101	18	1.1714104618561691
1101	19	1.147057541295979
1101	20	1.2038320063456425
1101	21	1.1563717364978032
1101	22	1.154172605488526
1101	23	1.1677739048571567
1101	24	1.290716546733293
1101	25	1.3684949522967145
1102	1	0.5895425629679067
1102	2	0.5653389211938062
1102	3	0.5863827025187014
1102	4	0.5060189118674856
1102	5	0.6054865474501696
1102	6	0.5675711127208558
1102	7	0.6570794794822348
1102	8	0.7350780745732663
1102	9	0.7820076141698138
1102	10	0.8030266479695882
1102	11	0.7573968270261631
1102	12	0.862550979175488
1102	13	0.8217080192970698
1102	14	0.8824545766307352
1102	15	0.9706826253224108
1102	16	0.8196930974954313
1102	17	0.8833982447128861
1102	18	0.9787701564690394
1102	19	0.9658606863078205
1102	20	0.9682588237823424
1102	21	0.9073222374632124
1102	22	0.9736947082567937
1102	23	0.9951972776389795
1102	24	0.9969932656694667
1102	25	1.1807100966425068
1103	1	0.48014575905114754
1103	2	0.47624042736103434
1103	3	0.5301743601882336
1103	4	0.4369348238112707
1103	5	0.4848812938026086
1103	6	0.4714443268134403
1103	7	0.4957585467423513
1103	8	0.4766213344862251
1103	9	0.5684530229562199
1103	10	0.6159790954348097
1103	11	0.5882781353355924
1103	12	0.6417087099458385
1103	13	0.589105436903111
1103	14	0.6332520476731531
1103	15	0.649957007930638
1103	16	0.7240062054614071
1103	17	0.6648935579129898
1103	18	0.7794060936387126
1103	19	0.827099335317115
1103	20	0.7085406950202682
1103	21	0.7532161813278861
1103	22	0.8200351407522888
1103	23	0.8548641133198558
1103	24	0.8638173209327817
1103	25	0.9488157440912985
1104	1	0.5503201518875365
1104	2	0.49534586656183777
1104	3	0.52775937936552
1104	4	0.48551805940759607
1104	5	0.5339862813761584
1104	6	0.5543151543924125
1104	7	0.6145383289479938
1104	8	0.5575854352506013
1104	9	0.6479780876678873
1104	10	0.6774531111046613
1104	11	0.662530602089447
1104	12	0.7031922134860977
1104	13	0.6821088824908585
1104	14	0.8136787882778762
1104	15	0.7846874719778754
1104	16	0.7965862299278612
1104	17	0.8362441161649556
1104	18	0.8451272776472791
1104	19	0.9151680250951415
1104	20	0.9191483976270973
1104	21	0.9338165349376979
1104	22	0.8721219891817498
1104	23	0.9072216145203456
1104	24	0.949007950161878
1104	25	1.064853173497383
1105	1	0.4954614694574069
1105	2	0.44523553023193685
1105	3	0.4941390032733821
1105	4	0.47091386869293217
1105	5	0.479233741423144
1105	6	0.5464051640334304
1105	7	0.543183259618047
1105	8	0.5301130274233756
1105	9	0.641443573911701
1105	10	0.7274921730175592
1105	11	0.7111662929514608
1105	12	0.7922221759633956
1105	13	0.7653790972287666
1105	14	0.7586970193165854
1105	15	0.7756536361832573
1105	16	0.7742767562725064
1105	17	0.7385325992481171
1105	18	0.8163590086211912
1105	19	0.8447130189025884
1105	20	0.8560228285926001
1105	21	0.9301352084509418
1105	22	0.91059418747016
1105	23	0.8342486672010452
1105	24	0.9094105618525035
1105	25	0.8942036684796904
1106	1	0.43453191714142037
1106	2	0.45099961392647003
1106	3	0.5034501591059914
1106	4	0.46557761768097805
1106	5	0.5015405725127451
1106	6	0.4535981872726751
1106	7	0.5075405067865546
1106	8	0.5725274992480394
1106	9	0.6475018819348222
1106	10	0.605915280571125
1106	11	0.6505050831300707
1106	12	0.6852626318592314
1106	13	0.6658853308161312
1106	14	0.6622141579146827
1106	15	0.6701154652120493
1106	16	0.5766508510851978
1106	17	0.6755348756224038
1106	18	0.739706517972003
1106	19	0.6776894272225036
1106	20	0.763494303890127
1106	21	0.7060457077855986
1106	22	0.7781140630356163
1106	23	0.7914211555297612
1106	24	0.8882041083618617
1106	25	0.94726289168851
1107	1	0.2877691304855112
1107	2	0.2452902888659665
1107	3	0.2541616036223857
1107	4	0.17246347468194045
1107	5	0.3215071507841216
1107	6	0.23940420954286168
1107	7	0.2319464527217363
1107	8	0.27882292847366585
1107	9	0.3437569902802764
1107	10	0.3495226898465944
1107	11	0.3132378335993593
1107	12	0.36532601953243926
1107	13	0.25200825375933533
1107	14	0.3521961512976546
1107	15	0.23282392142645136
1107	16	0.23463088966421708
1107	17	0.31760555853419703
1107	18	0.39059673379287574
1107	19	0.32209356256277033
1107	20	0.2753856177306133
1107	21	0.11974643697687526
1107	22	0.03341720376371882
1107	23	0.28004629138536075
1107	24	0.24892693217687878
1107	25	0.45759431348356117
1108	1	0.33831303964561954
1108	2	0.3226420665627643
1108	3	0.3322491197855051
1108	4	0.24972282888884934
1108	5	0.3242911116239995
1108	6	0.3307777562692209
1108	7	0.3550171673320719
1108	8	0.3204273052843192
1108	9	0.3693996720986519
1108	10	0.4012138902158
1108	11	0.3681815757464406
1108	12	0.3210896837870081
1108	13	0.3273481277327406
1108	14	0.39969351489781957
1108	15	0.41181792647483206
1108	16	0.40917300206243823
1108	17	0.47504242212239944
1108	18	0.378829974802386
1108	19	0.31672209576065313
1108	20	0.4242039689025887
1108	21	0.4329418555858098
1108	22	0.49161443742578115
1108	23	0.3990998818840481
1108	24	0.46553509914206614
1108	25	0.6659119790860544
1109	1	0.10052690856302604
1109	2	0.10481926374568573
1109	3	0.09795260360895242
1109	4	0.11400498605868137
1109	5	0.18452150076851837
1109	6	0.18847728594715818
1109	7	0.12198779318431718
1109	8	0.13269686065276431
1109	9	0.15923377797348337
1109	10	0.12154514093539603
1109	11	0.23445211982465253
1109	12	0.14367102952817135
1109	13	0.16334494034882852
1109	14	0.11739359597825683
1109	15	0.15781284559552944
1109	16	0.18735205424661672
1109	17	0.19452454766461358
1109	18	0.08593844607882062
1109	19	0.14471624603898903
1109	20	0.17961204837113343
1109	21	0.22948118064140033
1109	22	0.2372265392888906
1109	23	0.2249900327949348
1109	24	0.32159550684699667
1109	25	0.4589370235103658
1110	1	0.08947093336857392
1110	2	0.005254144187631482
1110	3	0.06838340967144774
1110	4	0.19015847189213275
1110	5	0.19635117096704136
1110	6	0.23621141803256052
1110	7	0.2231945881247981
1110	8	0.20989033526924317
1110	9	0.19268199926559504
1110	10	0.2410462981354371
1110	11	0.268202180728224
1110	12	0.34235135353646484
1110	13	0.32445147675819186
1110	14	0.28617653238455887
1110	15	0.32136113858351933
1110	16	0.33441433367337225
1110	17	0.33901421085509753
1110	18	0.35170166224003196
1110	19	0.29662363241155987
1110	20	0.34063490530560614
1110	21	0.36263446579838643
1110	22	0.3367169997430537
1110	23	0.4603695219454025
1110	24	0.3724360532989266
1110	25	0.50899143713076
1111	1	0.14239079815893163
1111	2	0.13111977081674198
1111	3	0.13166323485564746
1111	4	0.09709438920118885
1111	5	0.15322240798939646
1111	6	0.24801454421595537
1111	7	0.22208092777566435
1111	8	0.19864287181296447
1111	9	0.20192181231721662
1111	10	0.23567594476514842
1111	11	0.22583313550130413
1111	12	0.24696416663041987
1111	13	0.28704228713376523
1111	14	0.26927227360914685
1111	15	0.2532734689638403
1111	16	0.2921864175240714
1111	17	0.28629433351125044
1111	18	0.2827698277430031
1111	19	0.3123263289119578
1111	20	0.3657191038874288
1111	21	0.3440027862675876
1111	22	0.3422787722350975
1111	23	0.3721337377919198
1111	24	0.3798151981084885
1111	25	0.5265289489870852
1112	1	0.04846946639274918
1112	2	0.06283877632336399
1112	3	-0.014652004718069378
1112	4	0.0506198695541471
1112	5	0.09473483465622223
1112	6	0.16447335733553103
1112	7	0.14539813704093518
1112	8	0.1936724405218584
1112	9	0.16997192394057237
1112	10	0.17435132469584858
1112	11	0.13306283233931993
1112	12	0.15599091323152692
1112	13	0.09189956992118198
1112	14	0.17081892818797684
1112	15	0.14151943421729385
1112	16	0.18675977936684518
1112	17	0.1855178454977917
1112	18	0.2656578927739943
1112	19	0.24498575920821963
1112	20	0.24515495194031445
1112	21	0.24034097598987358
1112	22	0.20832481812972503
1112	23	0.2093390518420435
1112	24	0.1863694375916083
1112	25	0.31020314046887876
1113	1	-0.10319640434707367
1113	2	-0.0609138778024807
1113	3	-0.1394641742737015
1113	4	-0.08828926296020967
1113	5	-0.016063052987092874
1113	6	-0.03610207290024192
1113	7	-0.05587302445676556
1113	8	-0.1277305505049
1113	9	-0.19621853389147503
1113	10	-0.11145524767007231
1113	11	-0.1696750938235425
1113	12	-0.20154334271366992
1113	13	-0.2142102671542503
1113	14	-0.13740975320168047
1113	15	-0.0719880246950595
1113	16	-0.14028247771586422
1113	17	-0.09721539215850328
1113	18	-0.14762666516740097
1113	19	-0.08627356287767185
1113	20	-0.07882282097346405
1113	21	-0.17276541041105276
1113	22	-0.1012792621560763
1113	23	-0.0471498246657589
1113	24	-0.014614547697007652
1113	25	0.0494781091499803
1114	1	-0.44822847199738547
1114	2	-0.401720545535575
1114	3	-0.4429521591317247
1114	4	-0.3414019825083514
1114	5	-0.3181913041035713
1114	6	-0.3258547660161284
1114	7	-0.41286400059493644
1114	8	-0.46301219407320104
1114	9	-0.46756370538005143
1114	10	-0.5415801398621483
1114	11	-0.49948236317536043
1114	12	-0.5106538175315336
1114	13	-0.4675229675737924
1114	14	-0.506695278850195
1114	15	-0.6585693920299533
1114	16	-0.6069081844450182
1114	17	-0.5224928695459425
1114	18	-0.5116148871426063
1114	19	-0.4783595686897897
1114	20	-0.45937997669025776
1114	21	-0.4701143491663089
1114	22	-0.47151651447547493
1114	23	-0.5934987176422624
1114	24	-0.5873362245025717
1114	25	-0.5034151342726219
1115	1	-0.8252563920368452
1115	2	-0.7734615697927936
1115	3	-0.8324463996679441
1115	4	-0.7606578733708389
1115	5	-0.8997936910130804
1115	6	-0.8251265134054293
1115	7	-0.8250350304965721
1115	8	-0.8578782394322957
1115	9	-0.9307901116836135
1115	10	-1.0463366305593027
1115	11	-1.0370990743505928
1115	12	-1.0226415743067179
1115	13	-1.053640420360935
1115	14	-1.2633000543607906
1115	15	-1.2586146061795844
1115	16	-1.3494569516316304
1115	17	-1.3719684613257144
1115	18	-1.3360120369436572
1115	19	-1.3457485743129354
1115	20	-1.3284512733150322
1115	21	-1.3646308870742132
1115	22	-1.3296496481580036
1115	23	-1.3723255877094545
1115	24	-1.4093645043995195
1115	25	-1.241846582401834
1116	1	-2.0689490842042204
1116	2	-1.9481028836361638
1116	3	-2.0014944288351337
1116	4	-1.9646140042640425
1116	5	-2.401260324239594
1116	6	-2.2659355925729443
1116	7	-2.1908881623685765
1116	8	-2.3407007832017257
1116	9	-2.5035999270046077
1116	10	-2.536051386509058
1116	11	-2.5680998448219157
1116	12	-2.4821851921992177
1116	13	-2.600737690175677
1116	14	-2.8121658772786446
1116	15	-2.8480492668568402
1116	16	-2.8775815786041434
1116	17	-2.894725844164576
1116	18	-2.905581846013625
1116	19	-3.045328960218434
1116	20	-3.069090944639356
1116	21	-3.199178326475817
1116	22	-3.1157819410640215
1116	23	-3.106252100047932
1116	24	-3.1060643675633273
1116	25	-3.053850560847586
1201	1	0.6839019971299649
1201	2	0.6669264207095864
1201	3	0.6638213722583473
1201	4	0.5302497405855462
1201	5	0.6168431888413721
1201	6	0.6573832107316449
1201	7	0.7530933836752354
1201	8	0.7149597048592682
1201	9	0.7995116314110859
1201	10	0.8065686488172901
1201	11	0.7999874309087858
1201	12	0.8006151990502843
1201	13	0.7960074302336366
1201	14	0.8843314334472936
1201	15	0.9806708740257619
1201	16	0.931088589419808
1201	17	0.9538581682955254
1201	18	1.0326347666022428
1201	19	0.9797846014688858
1201	20	1.0699775922939523
1201	21	1.0455103181535677
1201	22	0.9978935856630002
1201	23	1.0292347556795178
1201	24	0.9720486927128889
1201	25	0.8977558529003744
1202	1	0.5621251004049661
1202	2	0.5804067667449821
1202	3	0.5687706707956153
1202	4	0.4528824924268591
1202	5	0.40243679019791756
1202	6	0.5042612921770129
1202	7	0.4931146049979276
1202	8	0.645081276671263
1202	9	0.597742184530837
1202	10	0.7282426663997086
1202	11	0.7359292553251038
1202	12	0.7220578560839286
1202	13	0.7012721088063003
1202	14	0.7684193073761065
1202	15	0.7498554521034038
1202	16	0.7766265688237723
1202	17	0.8292458331543884
1202	18	0.7978641492726624
1202	19	0.6694893412063365
1202	20	0.6901990234583835
1202	21	0.7258567254101536
1202	22	0.7194168031764363
1202	23	0.7997429827132905
1202	24	0.8013491959317278
1202	25	0.8200071667967563
1203	1	0.38357557286136057
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2211	6	0.017673532232819866
2211	7	-0.053951971645588515
2211	8	-0.08765707146230284
2211	9	-0.11104716003730886
2211	10	-0.023805147911694746
2211	11	-0.017942490472755424
2211	12	-0.08077443061522871
2211	13	-0.1061021783173004
2211	14	-0.013419420295861073
2211	15	-0.07330335619263906
2211	16	-0.03432864414116921
2211	17	-0.06312525605990515
2211	18	-0.056070439632705416
2211	19	0.011248098293037856
2211	20	0.08762188878706922
2211	21	0.004864012416447849
2211	22	-0.07356869110954278
2211	23	-0.0489527788826436
2211	24	-0.08276622728128302
2211	25	-0.1463226780585316
2212	1	-0.08504318201797645
2212	2	-0.10337306461964246
2212	3	-0.12065104521990122
2212	4	-0.08336639437722937
2212	5	0.021123342288014157
2212	6	-0.07723300034536607
2212	7	-0.08132891255831254
2212	8	-0.061098724121166015
2212	9	-0.04222989580468095
2212	10	-0.051655988641293504
2212	11	-0.08969133739822865
2212	12	-0.0907008639764868
2212	13	-0.07460632366504427
2212	14	-0.06904793396630993
2212	15	-0.06160591009165728
2212	16	-0.033497651296450215
2212	17	-0.033503503234044274
2212	18	-5.073945270197555E-4
2212	19	0.01117816115906578
2212	20	0.03783116828835631
2212	21	-0.02807191918054741
2212	22	-0.14657286379708268
2212	23	-0.18479239178860496
2212	24	-0.12671624601253484
2212	25	-0.14248628054979662
2213	1	-0.22309469513168168
2213	2	-0.16134622775161844
2213	3	-0.1823713744545543
2213	4	-0.18432817445562932
2213	5	-0.1589486558548927
2213	6	-0.16267595185840378
2213	7	-0.2362261304329678
2213	8	-0.20739990977115497
2213	9	-0.272546364956078
2213	10	-0.35378325635743835
2213	11	-0.34580087899688294
2213	12	-0.3041727651167463
2213	13	-0.20551936709127716
2213	14	-0.2439941078658947
2213	15	-0.37677334336643753
2213	16	-0.31802977704251134
2213	17	-0.3208078326952446
2213	18	-0.329162274494216
2213	19	-0.3116588831638083
2213	20	-0.3126908391878729
2213	21	-0.3437622374226308
2213	22	-0.39275044286301863
2213	23	-0.4063030485188861
2213	24	-0.40879467736162667
2213	25	-0.40363432605807503
2214	1	-0.24563408474511306
2214	2	-0.2562970161416942
2214	3	-0.2810791512280133
2214	4	-0.18691743326852617
2214	5	-0.31951928814517316
2214	6	-0.31829944836958646
2214	7	-0.3861149978007461
2214	8	-0.4603626794426461
2214	9	-0.43185852031938055
2214	10	-0.3980635126499692
2214	11	-0.38605399716692546
2214	12	-0.3848576266881665
2214	13	-0.46139687250742156
2214	14	-0.5164018301192002
2214	15	-0.4540898519724905
2214	16	-0.4337037824084433
2214	17	-0.4736505026044284
2214	18	-0.490248187969776
2214	19	-0.4615979664600758
2214	20	-0.4886641314876172
2214	21	-0.5429187891665563
2214	22	-0.5413526765153378
2214	23	-0.5685291923660003
2214	24	-0.5945874852450501
2214	25	-1.3812035881708766
2215	1	-0.7554745682803805
2215	2	-0.8218064787976438
2215	3	-0.7950515232225683
2215	4	-0.6637999317778025
2215	5	-0.7822775062234939
2215	6	-0.7832847218657051
2215	7	-0.7874257385886452
2215	8	-0.8369447228149198
2215	9	-0.9482964313703732
2215	10	-0.9639741144300942
2215	11	-0.9488115218872366
2215	12	-1.0202060054700723
2215	13	-1.0097345992092315
2215	14	-1.1878532763589504
2215	15	-1.0903002743619865
2215	16	-0.9935054694115095
2215	17	-1.1212324690769933
2215	18	-1.136820257492154
2215	19	-1.0782699278606245
2215	20	-1.1434315245833702
2215	21	-1.2337926219613848
2215	22	-1.2198993603100519
2215	23	-1.186199442222808
2215	24	-1.2605808067366624
2215	25	-1.3104085838783632
2216	1	-1.679508792551875
2216	2	-1.4661688441596006
2216	3	-1.5367197361199878
2216	4	-1.464303229888003
2216	5	-1.6845808488651386
2216	6	-1.4976539988791302
2216	7	-1.5606326520955847
2216	8	-1.735076721441871
2216	9	-1.884311006141452
2216	10	-1.8909719991864264
2216	11	-1.9262862642078815
2216	12	-1.8025683058903326
2216	13	-2.054303154442138
2216	14	-2.154784503107507
2216	15	-2.0243378384919097
2216	16	-2.0888895067437048
2216	17	-2.0662630380589846
2216	18	-2.072357510829697
2216	19	-2.108497038233665
2216	20	-2.0868026014093886
2216	21	-2.0284483846087795
2216	22	-2.1505059157099495
2216	23	-2.307453930157571
2216	24	-2.2495715664616824
2216	25	-2.3511372296585407
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96647.0
3	7.0
4	1766.0
5	14462.0
6	15954.0
7	12199.0
8	9690.0
9	8585.0
10	7935.0
11	7467.0
12	6967.0
13	6894.0
14	6716.0
15	6941.0
16	7251.0
17	7846.0
18	8611.0
19	10059.0
20	12330.0
21	15665.0
22	20210.0
23	24447.0
24	29632.0
25	35467.0
26	41696.0
27	48378.0
28	57770.0
29	71136.0
30	92965.0
31	122130.0
32	160680.0
33	214867.0
34	296796.0
35	417156.0
36	620024.0
37	1085355.0
38	2335950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33624901652541	17.682296311905	12.540014846234346	26.44143982533525
2	17.774265875337942	20.114977852281747	35.90702083931475	26.20373543306556
3	19.464155174362006	23.408061871437187	27.57390267737173	29.55388027682908
4	13.757988682018832	16.257513923167657	34.84507345354261	35.1394239412709
5	16.031674974796037	35.02453365508177	32.61541192523929	16.328379444882895
6	34.33872281910354	35.79938608900951	15.546170976654361	14.315720115232592
7	30.56108311578743	29.73576764615109	20.33788573685055	19.365263501210933
8	27.445830022463944	32.63953567098958	18.915643819955623	20.998990486590852
9	27.311560506110087	14.635815202505686	17.78668976416364	40.26593452722058
10	16.38585345478064	25.789283981890698	30.246072275520103	27.57879028780856
11	38.006116026624284	21.07135240866135	21.470137288862492	19.45239427585187
12	25.56408011026506	23.100751612734246	27.70465121332155	23.63051706367915
13	30.313554759549977	19.284652527061375	24.088870462244756	26.312922251143895
14	24.091470456392432	19.618782165423188	24.13008327773514	32.15966410044924
15	25.62321176612633	26.496281618618667	21.794381078900614	26.086125536354395
16	26.227239277853837	25.653297768257833	22.7946706273228	25.324792326565525
17	25.627912232136946	24.95653384870226	23.77681408981921	25.638739829341578
18	26.995390072355118	23.730939592704406	24.656781322114316	24.61688901282616
19	27.519566312282034	24.126910303366873	23.74552739334236	24.607995991008732
20	26.73237866546323	24.027488237849706	23.763012457607424	25.477120639079637
21	27.095701829525705	24.030085660983495	23.596905892474087	25.277306617016716
22	26.265880921441585	23.901488738772493	24.309780116730213	25.52285022305571
23	25.11082915378845	23.37957564393898	24.592983753027838	26.916611449244737
24	25.57758659883912	24.77042530297596	24.044680639680465	25.607307458504458
25	25.540968901538395	24.027793702072557	24.282816599335003	26.148420797054044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	277.0
2	437.5
3	598.0
4	598.0
5	598.0
6	1428.5
7	2259.0
8	2259.0
9	2259.0
10	3725.0
11	5191.0
12	5191.0
13	5191.0
14	9359.0
15	13527.0
16	13527.0
17	13527.0
18	26193.5
19	38860.0
20	38860.0
21	38860.0
22	63406.5
23	87953.0
24	87953.0
25	87953.0
26	127888.0
27	167823.0
28	167823.0
29	167823.0
30	208154.5
31	248486.0
32	248486.0
33	248486.0
34	310005.5
35	371525.0
36	371525.0
37	371525.0
38	420929.5
39	470334.0
40	470334.0
41	470334.0
42	532313.5
43	594293.0
44	594293.0
45	594293.0
46	640804.0
47	687315.0
48	687315.0
49	687315.0
50	708240.0
51	729165.0
52	729165.0
53	729165.0
54	688233.5
55	647302.0
56	647302.0
57	647302.0
58	611800.5
59	576299.0
60	576299.0
61	576299.0
62	517682.0
63	459065.0
64	459065.0
65	459065.0
66	395895.5
67	332726.0
68	332726.0
69	332726.0
70	265896.0
71	199066.0
72	199066.0
73	199066.0
74	165626.5
75	132187.0
76	132187.0
77	132187.0
78	111779.0
79	91371.0
80	91371.0
81	91371.0
82	70736.5
83	50102.0
84	50102.0
85	50102.0
86	36577.0
87	23052.0
88	23052.0
89	23052.0
90	16010.5
91	8969.0
92	8969.0
93	8969.0
94	4881.0
95	793.0
96	793.0
97	793.0
98	453.0
99	113.0
100	113.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030175203089051705
2	0.0019533055571037935
3	0.006769214085825215
4	0.15702219241373166
5	0.018404853223400398
6	0.0768861480494476
7	0.04612158552506285
8	0.019145762227819076
9	0.03497427277676361
10	0.0230860510240457
11	0.03564782641714423
12	0.028356608260024035
13	0.006567147993711029
14	0.0035193177709887313
15	0.003030991381712783
16	0.004058160683293227
17	0.0020543386031608864
18	0.00274473108455102
19	0.0
20	0.001869111352056216
21	1.3471072807612369E-4
22	0.0
23	1.0103304605709276E-4
24	0.0010271693015804432
25	0.13152818712532527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5938651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.2797771745625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.12916915646646	40.61737540755278
2	10.07552597712556	9.728882981842764
3	2.3263624376610115	3.3694878035263707
4	0.9852205702676984	1.902649184012795
5	0.4916452032643662	1.1868260431273043
6	0.35549449553270074	1.0297917018661378
7	0.22372985222473366	0.7561139188895557
8	0.1646660102169466	0.636003062519873
9	0.131836722519147	0.5728542827984139
>10	0.8861839076266207	8.3894578444074
>50	0.10758576983085293	3.6551273571934217
>100	0.09647449005497728	9.707430474623994
>500	0.012964869151621108	4.451593400472427
>1k	0.01236756523062739	11.631097189363313
>5k	7.378376984174197E-4	2.1644655127082446
>10k+	3.513512849606761E-5	0.2008438350951082
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	11840	0.19937187755266306	No Hit
GAATAGGACCGCGGTTCTATTTTGT	7746	0.13043366245970675	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	7033	0.11842756881992222	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	6901	0.1162048418066662	No Hit
GTATCTGATCGTCTTCGAACCTCCG	6713	0.11303913969687729	No Hit
CTTATGACCCGCACTTACTGGGAAT	6669	0.1122982306924586	No Hit
GAACTACGACGGTATCTGATCGTCT	6408	0.10790329318897507	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	6381	0.10744864448171815	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	6322	0.10645515286215675	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	6227	0.10485546296625278	No Hit
ATCAGATACCGTCGTAGTTCCGACC	6188	0.10419874816688167	No Hit
CTATTGGAGCTGGAATTACCGCGGC	6130	0.10322209538832978	No Hit
GTCCTATTCCATTATTCCTAGCTGC	6021	0.10138666171829258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.683884100951546E-5
2	0.0	0.0	0.0	0.0	1.683884100951546E-5
3	0.0	0.0	0.0	0.0	1.683884100951546E-5
4	0.0	0.0	0.0	1.683884100951546E-5	1.683884100951546E-5
5	0.0	0.0	0.0	1.683884100951546E-5	3.367768201903092E-5
6	1.683884100951546E-5	0.0	0.0	1.683884100951546E-5	5.051652302854638E-5
7	1.683884100951546E-5	0.0	0.0	1.683884100951546E-5	5.051652302854638E-5
8	1.683884100951546E-5	0.0	0.0	1.683884100951546E-5	5.051652302854638E-5
9	1.683884100951546E-5	0.0	0.0	6.735536403806184E-5	5.051652302854638E-5
10	1.683884100951546E-5	0.0	0.0	1.0103304605709276E-4	6.735536403806184E-5
11	1.683884100951546E-5	0.0	0.0	1.0103304605709276E-4	6.735536403806184E-5
12	1.683884100951546E-5	0.0	0.0	1.1787188706660822E-4	1.3471072807612369E-4
13	1.683884100951546E-5	0.0	0.0	1.1787188706660822E-4	1.5154956908563914E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2640	0.0	16.586157	1
GTATCAA	8860	0.0	13.376037	1
CTCGACG	350	0.0	13.289249	13
ACGCTTA	360	0.0	12.919669	17
TCGACGC	355	0.0	12.834471	14
GACGCTT	390	0.0	12.169436	16
AACCGCG	625	0.0	11.851107	7
GCGTTAT	410	0.0	11.840722	1
CGCTTAC	405	0.0	11.48415	18
ACGGTAT	1200	0.0	11.473366	9
CGTTATT	425	0.0	11.197228	2
CGACCAT	1140	0.0	11.160835	10
ACCGTCC	345	0.0	10.733704	8
CGGTTCT	1490	0.0	10.641463	12
CGACGCT	440	0.0	10.570815	15
CCGACCA	1285	0.0	10.566642	9
TCCGCGA	135	5.7423676E-8	10.550043	10
CGTCGTA	1025	0.0	10.467702	10
TCGCGTA	695	0.0	10.383239	9
AGAACCG	790	0.0	10.342818	5
>>END_MODULE