FastQCFastQC Report
Thu 2 Feb 2017
SRR4050248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498597
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA400158.025519608020105No Hit
CTTCCGTACTCTGCGTTGATACCAC242204.8576305112144675No Hit
GAGTACGGAAGCAGTGGTATCAACG202064.0525715156729785No Hit
TCCGTACTCTGCGTTGATACCACTG187713.7647639275807916No Hit
GCTTCCGTACTCTGCGTTGATACCA176983.5495600655439166No Hit
GTACATGGGAAGCAGTGGTATCAAC111782.241890745431681No Hit
CCCATGTACTCTGCGTTGATACCAC102322.0521583563479124No Hit
GTACATGGAAGCAGTGGTATCAACG95041.9061486531206566No Hit
GCGTTGATACCACTGCTTCCGTACT90221.8094773935663473No Hit
GTATCAACGCAGAGTACGGAAGCAG83641.6775070848801736No Hit
TCCATGTACTCTGCGTTGATACCAC69021.3842843017507125No Hit
TATCAACGCAGAGTACGGAAGCAGT59001.1833203970340778No Hit
ACTCTGCGTTGATACCACTGCTTCC55731.1177363682493076No Hit
CAGTGGTATCAACGCAGAGTACGGA41140.8251152734573213No Hit
GGTATCAACGCAGAGTACGGAAGCA39310.7884122848713491No Hit
GAGTACATGGGAAGCAGTGGTATCA38180.765748690826459No Hit
ATACCACTGCTTCCGTACTCTGCGT38090.7639436258140342No Hit
CATGTACTCTGCGTTGATACCACTG38000.7621385608016092No Hit
ACGCAGAGTACGGAAGCAGTGGTAT36010.722226567749104No Hit
GTACGGGAAGCAGTGGTATCAACGC32500.6518290322645343No Hit
AAGCAGTGGTATCAACGCAGAGTAC29940.6004849608000048No Hit
GAGTACATGGAAGCAGTGGTATCAA29720.5960725796585218No Hit
GAAGCAGTGGTATCAACGCAGAGTA27110.5437256942982007No Hit
GTGGTATCAACGCAGAGTACGGAAG26790.5373076853651346No Hit
GTACTCTGCGTTGATACCACTGCTT26430.5300874253154352No Hit
GTATCAACGCAGAGTACATGGGAAG25200.505418203478962No Hit
GCTTCCCATGTACTCTGCGTTGATA22820.45768426203928225No Hit
GCGTTGATACCACTGCTTCCCATGT22760.4564808853643323No Hit
GTTGATACCACTGCTTCCGTACTCT20860.4183739573242518No Hit
GATACCACTGCTTCCGTACTCTGCG20690.41496439007856045No Hit
CCACTGCTTCCGTACTCTGCGTTGA20230.4057385022372778No Hit
CCCGTACTCTGCGTTGATACCACTG19890.3989193677458949No Hit
TTCCCGTACTCTGCGTTGATACCAC19420.38949291712545403No Hit
ATCAACGCAGAGTACGGAAGCAGTG19350.3880889776713458No Hit
CATGGAAGCAGTGGTATCAACGCAG17680.35459499355190666No Hit
TATCAACGCAGAGTACATGGGAAGC17590.35278992853948177No Hit
CGGAAGCAGTGGTATCAACGCAGAG17560.35218824020200684No Hit
GCTTCCATGTACTCTGCGTTGATAC17490.35078430074789857No Hit
CTGCTTCCGTACTCTGCGTTGATAC16450.32992577171543347No Hit
CTTCCCGTACTCTGCGTTGATACCA16160.3241094511198423No Hit
GAGTACGGGAAGCAGTGGTATCAAC15950.3198976327575176No Hit
CAGTGGTATCAACGCAGAGTACATG14860.2980362898292609No Hit
ACGCAGAGTACATGGGAAGCAGTGG14430.28941209032545323No Hit
GCGTTGATACCACTGCTTCCATGTA13930.27938395136753735No Hit
AAAAAGTACTCTGCGTTGATACCAC13540.27156200298036287No Hit
GTATCAACGCAGAGTACATGGAAGC13370.2681524357346715No Hit
CCATGTACTCTGCGTTGATACCACT12210.24488715335230657No Hit
CACTGCTTCCGTACTCTGCGTTGAT12160.24388433945651497No Hit
GCAGAGTACGGAAGCAGTGGTATCA11710.23485901439439066No Hit
CCCCATGTACTCTGCGTTGATACCA11410.22884213101964113No Hit
GCAGTGGTATCAACGCAGAGTACGG11410.22884213101964113No Hit
GCCCATGTACTCTGCGTTGATACCA11280.226234814890583No Hit
TGATACCACTGCTTCCGTACTCTGC11060.22182243374909996No Hit
GGTATCAACGCAGAGTACATGGGAA10600.21259654590781735No Hit
GTATCAACGCAGAGTACGGGAAGCA10500.21059091811623415No Hit
GCTTCCCGTACTCTGCGTTGATACC10440.20938754144128424No Hit
GTACAAGCAGTGGTATCAACGCAGA9880.1981560258084184No Hit
GCGTTGATACCACTGCTTCCCGTAC9700.1945458957835687No Hit
TATCAACGCAGAGTACATGGAAGCA9430.1891307007462941No Hit
TACCACTGCTTCCGTACTCTGCGTT9300.18652338461723597No Hit
CTGCGTTGATACCACTGCTTCCGTA9050.181509315138278No Hit
AAAGTACTCTGCGTTGATACCACTG8870.17789918511342828No Hit
GGGAAGCAGTGGTATCAACGCAGAG8490.1702777995054122No Hit
ACGCAGAGTACATGGAAGCAGTGGT8470.16987667394709555No Hit
TCCCATGTACTCTGCGTTGATACCA8450.1694755483887789No Hit
ATACCACTGCTTCCCATGTACTCTG8200.16446147890982094No Hit
CTGCTTCCCATGTACTCTGCGTTGA8060.1616536000016045No Hit
TACGGAAGCAGTGGTATCAACGCAG7740.15523559106853832No Hit
ACGGAAGCAGTGGTATCAACGCAGA7670.1538316516144301No Hit
CATGGGAAGCAGTGGTATCAACGCA7550.15142489826453026No Hit
TCAACGCAGAGTACGGAAGCAGTGG7470.1498203960312637No Hit
CTCTGCGTTGATACCACTGCTTCCG7290.14621026600641399No Hit
TATCAACGCAGAGTACGGGAAGCAG7260.14560857766893906No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7260.14560857766893906No Hit
TACCACTGCTTCCCATGTACTCTGC7160.14360294987735586No Hit
GTATCAACGCAGAGTACTTTTTTTT6950.13939113151503119No Hit
GTGGTATCAACGCAGAGTACATGGG6580.1319703086861734No Hit
GCAGTGGTATCAACGCAGAGTACAT6510.1305663692320652No Hit
CTTCCCATGTACTCTGCGTTGATAC6470.1297641181154319No Hit
ATACCACTGCTTCCATGTACTCTGC6280.12595342531142387No Hit
GGTATCAACGCAGAGTACATGGAAG5900.11833203970340776No Hit
GCTTGTACTCTGCGTTGATACCACT5640.1131174074452915No Hit
GCAGAGTACATGGGAAGCAGTGGTA5460.10950727742044176No Hit
ACATGGGAAGCAGTGGTATCAACGC5270.10569658461643372No Hit
GTTGATACCACTGCTTCCCATGTAC5080.10188589181242566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA250.00614402418.936544
TCCGGAG400.005390756314.2024053
AAAAAGT2150.014.1107271
AAAAGTA2200.013.7720292
GTACTAT500.001483748613.3157446
ACGAAGC500.001535270213.2555783
CCCCGTA1153.947207E-1013.1732452
CTTCGTA802.0678563E-613.0188712
GCCCATG1302.0372681E-1012.3977671
GCTTCGT854.018684E-612.2690711
CTGTTCT550.003036355112.1052239
TACGAAG550.003141131712.0505252
TACGAAA550.003141131712.0505252
CGAAGCA550.003141131712.0505254
AGCCCGT658.144743E-411.6684871
GTACGAA1157.2313924E-811.5416561
CCCCATG2150.011.4649661
TGGGAGG855.2421303E-511.1897036
TCGTACT1159.1550646E-710.7032614
AGTACAA1605.711627E-1010.6518042
Produced by FastQC (version 0.11.5)