##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050248_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498597
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.582141489018184	31.0	31.0	34.0	27.0	34.0
2	30.654069318507734	31.0	31.0	34.0	27.0	34.0
3	30.815189421516777	31.0	31.0	34.0	27.0	34.0
4	34.28067156440974	37.0	35.0	37.0	30.0	37.0
5	34.301375660102245	37.0	35.0	37.0	30.0	37.0
6	34.319701081233944	37.0	35.0	37.0	30.0	37.0
7	34.18344274032936	37.0	35.0	37.0	30.0	37.0
8	34.11099946449738	37.0	35.0	37.0	30.0	37.0
9	35.56888629494361	39.0	37.0	39.0	30.0	39.0
10	35.56549678397584	39.0	37.0	39.0	30.0	39.0
11	35.395299209582085	39.0	37.0	39.0	30.0	39.0
12	35.37594490139331	39.0	37.0	39.0	30.0	39.0
13	35.29185093372002	39.0	37.0	39.0	30.0	39.0
14	36.482969211607774	40.0	37.0	41.0	30.0	41.0
15	36.426915926088604	40.0	37.0	41.0	30.0	41.0
16	36.24022607436467	40.0	37.0	41.0	29.0	41.0
17	36.34602294037068	40.0	37.0	41.0	30.0	41.0
18	36.21592187678626	40.0	37.0	41.0	29.0	41.0
19	36.336588467239075	40.0	37.0	41.0	29.0	41.0
20	36.3778763209566	40.0	37.0	41.0	30.0	41.0
21	36.334893711755186	40.0	37.0	41.0	30.0	41.0
22	36.33598878452939	40.0	37.0	41.0	30.0	41.0
23	36.22216339047367	40.0	37.0	41.0	29.0	41.0
24	36.12863494966877	40.0	37.0	41.0	29.0	41.0
25	35.8741127604057	40.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2917934661930701
1101	2	0.2538528437030827
1101	3	0.2927838147348538
1101	4	0.3526869261842194
1101	5	0.23688190190167546
1101	6	0.2906437408675444
1101	7	0.35109487271846973
1101	8	0.4631247136173542
1101	9	0.6405326318561393
1101	10	0.6018401953693555
1101	11	0.5020411290609275
1101	12	0.6388065448860303
1101	13	0.5876576220075975
1101	14	0.7482296378618685
1101	15	0.8419210069741325
1101	16	0.913444095004941
1101	17	0.8851377074044322
1101	18	0.8420760060667973
1101	19	0.8346008049071614
1101	20	0.7924347449874602
1101	21	0.6108735370226483
1101	22	0.7887466688741114
1101	23	0.9060283152065551
1101	24	0.6909282580479896
1101	25	0.773585452886465
1102	1	0.6483950448221272
1102	2	0.7346785143291719
1102	3	0.7012176755913977
1102	4	0.8551189469630458
1102	5	0.7010183760454964
1102	6	0.7688977572569158
1102	7	0.5424184094369267
1102	8	0.7488147684598943
1102	9	0.8909682368459357
1102	10	0.8058541004631508
1102	11	0.7090104662388939
1102	12	0.7181491882587565
1102	13	0.9497520210783037
1102	14	0.833188458841498
1102	15	0.8943529420882612
1102	16	1.0392958162657138
1102	17	0.9819371029543262
1102	18	0.9667455544939116
1102	19	1.0543393219827024
1102	20	0.8171627167386717
1102	21	1.1117875793512866
1102	22	1.0497207450231727
1102	23	1.1964162220924592
1102	24	1.2299802239671465
1102	25	1.152547231011475
1103	1	0.6526465770075802
1103	2	0.6111633350035071
1103	3	0.6913345145094318
1103	4	0.7787825649022224
1103	5	0.7986332769184017
1103	6	0.8244092795001094
1103	7	0.6291107313018998
1103	8	0.7314922951319147
1103	9	0.92587390706192
1103	10	0.9818285982226627
1103	11	0.9875275401557602
1103	12	0.9781774854558023
1103	13	0.8445344211951067
1103	14	0.9022297956928611
1103	15	0.9356880552944347
1103	16	0.9829343352168465
1103	17	1.0470345614210643
1103	18	1.237440921610066
1103	19	1.0377138496385214
1103	20	1.07996163630024
1103	21	1.0371441622735489
1103	22	0.9486243155454233
1103	23	1.1191481878438125
1103	24	1.160525205733883
1103	25	1.2198851739373495
1104	1	0.4646946872087234
1104	2	0.49554807954656255
1104	3	0.5134329965023738
1104	4	0.43238226335679997
1104	5	0.5998326052945089
1104	6	0.5223327280831924
1104	7	0.4478202539692049
1104	8	0.6337950056140471
1104	9	0.5467559274162355
1104	10	0.5288731104386812
1104	11	0.8839775281624753
1104	12	0.728388224584748
1104	13	0.6501472779953019
1104	14	0.5219330475860815
1104	15	0.5350644045388577
1104	16	0.7301296201429963
1104	17	0.5796220267262058
1104	18	0.6186183194101531
1104	19	0.8512525536785489
1104	20	0.968828442317438
1104	21	1.0408483736294727
1104	22	0.9717233800692853
1104	23	1.09026150724209
1104	24	1.0781827460827174
1104	25	1.0714254542375201
1105	1	0.18377740629144412
1105	2	0.5290686130670963
1105	3	0.46421104728042195
1105	4	0.5855354165099556
1105	5	0.3640071694690761
1105	6	0.43891164466210597
1105	7	0.4203720265209796
1105	8	0.20872188054092078
1105	9	0.5212733019336113
1105	10	0.6642890458546162
1105	11	0.829876674061623
1105	12	0.8728131690096959
1105	13	0.8835859114339328
1105	14	0.9091215347745703
1105	15	0.8235983930728423
1105	16	0.8668325568459352
1105	17	0.7873557344599149
1105	18	0.912218413010244
1105	19	0.7915824940084875
1105	20	0.8176408911298907
1105	21	0.7172377500188531
1105	22	0.8854474937934
1105	23	0.995959412939996
1105	24	0.9644001323000992
1105	25	1.0820724648845328
1106	1	0.15102248008337327
1106	2	0.26804195281065546
1106	3	0.05144710049850332
1106	4	0.04929038732525726
1106	5	0.1017348109194387
1106	6	-0.14452288100604704
1106	7	-0.03557337422545004
1106	8	-0.028324261511642135
1106	9	-0.07073902948822308
1106	10	-0.14635820510072506
1106	11	0.03337652454478501
1106	12	-0.06365952227686478
1106	13	-0.0026243406299784056
1106	14	0.042858976599305265
1106	15	-0.061539633271856076
1106	16	-0.09621117641602694
1106	17	0.12715216026403908
1106	18	0.13267396851072988
1106	19	0.021017320362439307
1106	20	0.19966384568172657
1106	21	0.04579810938793827
1106	22	0.07234188864671864
1106	23	-0.02260380459626532
1106	24	0.2221985505606483
1106	25	0.026946185111270893
1107	1	0.28287650539054354
1107	2	0.4615864659389324
1107	3	0.34688892110873937
1107	4	0.33250868560711666
1107	5	0.40171744611740223
1107	6	0.3510880435641681
1107	7	0.18614918167866534
1107	8	0.046126921485786454
1107	9	0.17709726749208698
1107	10	0.17571830064670024
1107	11	0.36171571917500245
1107	12	0.48269232756141633
1107	13	0.41771422432331207
1107	14	0.5238753530682132
1107	15	0.5813004693589932
1107	16	0.5248888432208076
1107	17	0.3422355194281934
1107	18	0.5558898973896973
1107	19	0.6336913538164595
1107	20	0.5814476814853506
1107	21	0.33877964589702714
1107	22	0.5215222537619653
1107	23	0.5279123519725815
1107	24	0.6025477615981742
1107	25	0.6856077321366172
1108	1	0.3778221003361395
1108	2	0.4218380058364879
1108	3	0.38184296491234093
1108	4	0.30532013629467514
1108	5	0.23103653649844347
1108	6	0.3766675824180439
1108	7	0.12693573308468586
1108	8	0.3497655215845583
1108	9	0.3747306553909624
1108	10	0.33118971275528253
1108	11	0.24663109081603807
1108	12	0.14126993746646122
1108	13	0.36316939101741497
1108	14	0.39543252108556004
1108	15	0.43580150527595407
1108	16	0.5549102449236258
1108	17	0.19948864659282606
1108	18	0.49464499543682905
1108	19	0.3788060812320708
1108	20	0.4030509765399728
1108	21	0.37822991101101167
1108	22	0.4068585152044193
1108	23	0.4302053471859324
1108	24	0.4088250767250443
1108	25	0.42611130892337457
1109	1	0.011728734242772987
1109	2	0.1761377601362426
1109	3	0.07103215410153041
1109	4	0.051020381994916875
1109	5	0.10204390750580927
1109	6	0.14939485514531725
1109	7	0.04462615077510179
1109	8	0.0315837034027453
1109	9	-0.17195239129208062
1109	10	-0.0103336287680591
1109	11	0.4063362505211998
1109	12	0.06387436007088354
1109	13	0.06587209372011671
1109	14	-0.04928492363188752
1109	15	-3.1643084198407223E-4
1109	16	0.1998733898867684
1109	17	0.16754761465179513
1109	18	0.0683550691469037
1109	19	0.19575939818538757
1109	20	0.16840724189624012
1109	21	0.09321762599872585
1109	22	0.06287817122407802
1109	23	0.32701124399182646
1109	24	0.41742458532455373
1109	25	0.2295511123631826
1110	1	-0.06283434212535965
1110	2	-0.14021387209986713
1110	3	-0.15030651308551413
1110	4	-0.2638529094126696
1110	5	-0.12501150366512093
1110	6	-0.1596182932180099
1110	7	-0.28588502991658515
1110	8	-0.36142433581468225
1110	9	-0.4026681743544387
1110	10	-0.5804084623807952
1110	11	-0.36601700197645926
1110	12	-0.18113402212161134
1110	13	-0.12322543833770538
1110	14	-0.06813524753636102
1110	15	-0.09470257937975646
1110	16	-0.10151743316470174
1110	17	-0.16939730532561725
1110	18	-0.22154608898529915
1110	19	-0.03428669648164373
1110	20	-0.18465898481619547
1110	21	-0.3373831421471678
1110	22	-0.1302567096364342
1110	23	-0.41371586316127207
1110	24	-0.32528202019793895
1110	25	-0.08696609404572087
1111	1	0.05281203304578597
1111	2	0.1632224479335207
1111	3	-9.329226141403524E-4
1111	4	0.05063811676704688
1111	5	-0.1034280295575769
1111	6	-0.015486934439557842
1111	7	-0.1667986188444388
1111	8	-0.26564356057036775
1111	9	-0.26230413366283045
1111	10	-0.08281520124963038
1111	11	-0.16465127771098764
1111	12	-0.2930388044432277
1111	13	-0.27986783753050304
1111	14	-0.24717575335169784
1111	15	-0.2571403028677537
1111	16	-0.28739513538650385
1111	17	-0.11965612979660989
1111	18	-0.15739735137986344
1111	19	-0.05851701167296852
1111	20	0.0015674372892178212
1111	21	-0.14223149187319706
1111	22	-0.3286868984882574
1111	23	-0.3032974278845444
1111	24	-0.2110019409642021
1111	25	-0.08201255668267748
1112	1	-0.4331038369243174
1112	2	-0.3762841214671333
1112	3	-0.576029498298201
1112	4	-0.561856001344097
1112	5	-0.4417289174058041
1112	6	-0.6172584043905047
1112	7	-0.5309784088152227
1112	8	-0.7447124892635557
1112	9	-0.5829509019347228
1112	10	-0.6290785464453634
1112	11	-0.4875958569340426
1112	12	-0.6134305734618053
1112	13	-0.7164223327024217
1112	14	-0.5428495008263567
1112	15	-0.6713520734078742
1112	16	-0.5044192236585161
1112	17	-0.6395164860066131
1112	18	-0.6604376187205148
1112	19	-0.6021528783745751
1112	20	-0.6394598293160243
1112	21	-0.7308035805847553
1112	22	-0.6133731366998276
1112	23	-0.8310923464961064
1112	24	-0.7482642351685342
1112	25	-0.46019476421543715
1113	1	-0.3301905223684294
1113	2	-0.19413309236209741
1113	3	-0.224407835651256
1113	4	-0.37432051254028664
1113	5	-0.1050575282686097
1113	6	-0.2922484253605191
1113	7	-0.11585919454436322
1113	8	-0.06387841101671654
1113	9	-0.14454400487895924
1113	10	-0.4335261017235652
1113	11	-0.011196965590215768
1113	12	-0.07050387857275808
1113	13	-0.19578120110853092
1113	14	0.010518632617170454
1113	15	0.019683103375562894
1113	16	0.09779134230709019
1113	17	0.0894853861156335
1113	18	0.08534448424058638
1113	19	-0.10777065060532465
1113	20	-0.1142761308345257
1113	21	0.03154511764375201
1113	22	-0.13553642174027658
1113	23	-0.015219504637023817
1113	24	0.011955134831403313
1113	25	-0.13487417030854942
1114	1	-0.30013047570940543
1114	2	-0.13664392392076863
1114	3	-0.2292770943602811
1114	4	-0.18059236630436004
1114	5	-0.22313846416047056
1114	6	-0.3398253760296228
1114	7	-0.20731031272274691
1114	8	-0.42110801746537874
1114	9	-0.6700327666072639
1114	10	-0.20498445035570967
1114	11	0.040029104714648156
1114	12	-0.032882923813936316
1114	13	-0.03189844743540249
1114	14	-0.48226644779338557
1114	15	-0.11942687295599796
1114	16	-0.23512586334847896
1114	17	-0.03297336358074432
1114	18	-0.23702925113610718
1114	19	-0.37573380918386334
1114	20	-0.24019900222847923
1114	21	-0.25486699562582515
1114	22	-0.34183171799117673
1114	23	-0.28447341218889477
1114	24	-0.5128656572422088
1114	25	-0.1709007535237248
1115	1	-0.4367342180762215
1115	2	-0.44415745913070026
1115	3	-0.3304824710634797
1115	4	-0.2009265871757009
1115	5	-0.27436697404645827
1115	6	-0.36410602596481567
1115	7	-0.5611070654979571
1115	8	-0.4703648448182989
1115	9	-0.17549893854042864
1115	10	-0.4607072320979597
1115	11	-0.2565459586335024
1115	12	-0.12108929018839376
1115	13	-0.24003734458006676
1115	14	-0.3176255942861843
1115	15	-0.4350921741010154
1115	16	-0.36980694714575435
1115	17	-0.47119091354689147
1115	18	-0.30389027739150265
1115	19	-0.5569202503811468
1115	20	-0.29187332378278086
1115	21	-0.5321526613252843
1115	22	-0.5312679730506034
1115	23	-0.5917402955823334
1115	24	-0.4881545156273006
1115	25	-0.6801019386780425
1116	1	-0.6379027153886767
1116	2	-0.45217919318071154
1116	3	-0.7414371333677572
1116	4	-0.3091761782016391
1116	5	-0.4638037583418537
1116	6	-0.8315633758129124
1116	7	-0.7089979278489764
1116	8	-0.9184777216586824
1116	9	-0.8516206959603849
1116	10	-0.4328152012496318
1116	11	-0.6728406536557046
1116	12	-0.7264819052853824
1116	13	-0.8293981765501499
1116	14	-0.5137812381282032
1116	15	-0.6672083227338703
1116	16	-0.6046106395972615
1116	17	-0.6437545966504175
1116	18	-0.5385245377327053
1116	19	-0.8662799138539228
1116	20	-0.8548728563838566
1116	21	-0.7698659120848106
1116	22	-1.0169129835670745
1116	23	-1.3707774537968689
1116	24	-1.7776614097614427
1116	25	-1.719158961346892
1201	1	0.22130656491435374
1201	2	0.141736526516258
1201	3	0.2499454423898193
1201	4	0.15559654907108467
1201	5	0.20111101438541823
1201	6	0.10001806358102527
1201	7	0.3257321857873876
1201	8	0.2464370510685896
1201	9	0.10174104646385729
1201	10	0.020049382083705325
1201	11	0.2011413539200504
1201	12	-0.14892508710356367
1201	13	-0.11301560079257911
1201	14	-0.047019874491837754
1201	15	-0.039225368188418486
1201	16	0.0803088679784949
1201	17	0.016969835167763847
1201	18	0.042678113782450566
1201	19	0.023634858873350595
1201	20	-0.19170997759597697
1201	21	0.03264355761216109
1201	22	-0.009289877506468258
1201	23	-0.06123673409990005
1201	24	-0.06973053854932232
1201	25	-0.2125301734681031
1202	1	0.7999271966347834
1202	2	0.8353134754409908
1202	3	0.8365247434650875
1202	4	1.060032032942054
1202	5	1.0224719014821915
1202	6	0.9337445420756509
1202	7	0.9083481132067419
1202	8	0.7889269704234252
1202	9	0.9098895679692305
1202	10	0.9758944761697279
1202	11	0.9595200501566126
1202	12	1.006377332251276
1202	13	0.8324346680246251
1202	14	1.0817906093791265
1202	15	1.1133048737470688
1202	16	1.129040386795701
1202	17	1.152902294845184
1202	18	1.1061088933523422
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2210	18	0.12227229255564964
2210	19	-0.10505385178040427
2210	20	0.010254812557370485
2210	21	0.02321685260812245
2210	22	0.09970048770740902
2210	23	-0.11213778837754518
2210	24	-0.2659730980731325
2210	25	-0.28549728404114916
2211	1	-0.10752050852191175
2211	2	-0.2560174570546039
2211	3	-0.20283316014383246
2211	4	-0.2629010756320014
2211	5	-0.17112399155282532
2211	6	-0.31002375154169926
2211	7	0.016099243388339346
2211	8	-0.2751447482188212
2211	9	-0.21479819542054912
2211	10	-0.2942166264277759
2211	11	-0.2501619665470898
2211	12	-0.11251623912256292
2211	13	-0.10409586603406495
2211	14	-0.5583396488386327
2211	15	-0.5493018230577746
2211	16	-0.45604121614660187
2211	17	-0.5745254884664419
2211	18	-0.43887003546537784
2211	19	-0.6642177242620448
2211	20	-0.7704040591478432
2211	21	-0.5543455061249745
2211	22	-0.5923448557329181
2211	23	-0.5123235561505766
2211	24	-0.5257287818193106
2211	25	-0.6283308464688986
2212	1	-0.8536428505532747
2212	2	-0.9489690340353008
2212	3	-0.8613030588092236
2212	4	-0.8418554892982186
2212	5	-0.8138582601949196
2212	6	-0.8364140407355194
2212	7	-0.8514167200879115
2212	8	-0.8149950532479551
2212	9	-0.8049398588119274
2212	10	-0.8561964962136557
2212	11	-0.9417037450008081
2212	12	-0.8749068017318606
2212	13	-0.825283286643895
2212	14	-1.1931904380410003
2212	15	-0.9784302542795444
2212	16	-0.980907783700161
2212	17	-0.9290826978058604
2212	18	-1.2455225756659942
2212	19	-0.9250874133089013
2212	20	-0.7956892941427398
2212	21	-0.7713320119322091
2212	22	-0.7580346317031115
2212	23	-0.9226632179128487
2212	24	-0.979231063129653
2212	25	-1.0430907250268575
2213	1	-0.35646775690453936
2213	2	-0.5623612242643823
2213	3	-0.35652655214570217
2213	4	-0.4692570809054928
2213	5	-0.33908585329608343
2213	6	-0.46925764875308573
2213	7	-0.45841777030160813
2213	8	-0.47203850513749757
2213	9	-0.7092159695392652
2213	10	-0.47977070476250816
2213	11	-0.3671189163765902
2213	12	-0.4605834594689
2213	13	-0.6758693283491723
2213	14	-0.45096454662298413
2213	15	-0.6671710649097307
2213	16	-0.6122378484539794
2213	17	-0.6148638153006232
2213	18	-0.5938790193166952
2213	19	-0.5660350767238356
2213	20	-0.6080717067138579
2213	21	-0.625832486494005
2213	22	-0.7899117061559622
2213	23	-0.5681374708290221
2213	24	-0.6254578755906905
2213	25	-0.5500350524688855
2214	1	-0.8488225647700673
2214	2	-0.8562001722016888
2214	3	-0.7801272893656055
2214	4	-1.003796942053178
2214	5	-1.059123822892687
2214	6	-0.9112029669909631
2214	7	-0.8188822742717861
2214	8	-0.8083244134284513
2214	9	-1.072855230656458
2214	10	-1.1981998166342436
2214	11	-1.3027895509122942
2214	12	-1.0188030456834483
2214	13	-1.169863480476998
2214	14	-1.01020404608947
2214	15	-1.2151776610878287
2214	16	-0.9712459273863914
2214	17	-1.0932973615777968
2214	18	-1.2877983408526603
2214	19	-1.4086413985231019
2214	20	-1.1998029263139287
2214	21	-1.262348429567325
2214	22	-1.3073513913131762
2214	23	-1.3574907405101584
2214	24	-1.1041810439733837
2214	25	-1.2115069979256958
2215	1	-1.0541103049382698
2215	2	-1.0985528780134324
2215	3	-1.1480484349400477
2215	4	-1.196077703204601
2215	5	-1.2195550291776485
2215	6	-0.992841304646106
2215	7	-0.9193862716248375
2215	8	-1.1077465477610602
2215	9	-1.0837846633324162
2215	10	-1.0015408189349344
2215	11	-1.059727401498236
2215	12	-1.1505302561542763
2215	13	-1.2627975145333679
2215	14	-1.2738769941277255
2215	15	-1.2374027901650138
2215	16	-1.2243972574007813
2215	17	-1.3138219805019347
2215	18	-1.2931645661048492
2215	19	-1.4046709766273011
2215	20	-1.5248604435691036
2215	21	-1.4112330949669456
2215	22	-1.6481844104496872
2215	23	-1.6635425560548214
2215	24	-1.5587466444227474
2215	25	-1.8388982922370687
2216	1	-0.4654700554560165
2216	2	-0.3838711123726313
2216	3	-0.44392029835092117
2216	4	-0.4393108583363201
2216	5	-0.358206115244208
2216	6	-0.4454101771597152
2216	7	-0.5361023049533529
2216	8	-0.21334304152400563
2216	9	-0.3129001572398522
2216	10	-0.32355594199037085
2216	11	-0.14767230348735438
2216	12	-0.5175593463628232
2216	13	-0.14021467486664818
2216	14	-0.07190413375109728
2216	15	-0.21081101633656374
2216	16	-0.34981265979928367
2216	17	-0.28323271112853377
2216	18	-0.5421019788101447
2216	19	-0.6329044929785042
2216	20	-0.40525164426264837
2216	21	-0.5677615349804341
2216	22	-0.5159449700990635
2216	23	-0.6302471507665715
2216	24	-0.8545974366974676
2216	25	-0.6187801734681031
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12247.0
3	0.0
4	93.0
5	700.0
6	1375.0
7	1296.0
8	1259.0
9	1083.0
10	980.0
11	1391.0
12	1446.0
13	1244.0
14	593.0
15	550.0
16	476.0
17	546.0
18	707.0
19	908.0
20	1276.0
21	1625.0
22	1943.0
23	2324.0
24	2616.0
25	2866.0
26	3189.0
27	3479.0
28	3973.0
29	4602.0
30	5632.0
31	7446.0
32	9911.0
33	13273.0
34	18706.0
35	27527.0
36	43755.0
37	86466.0
38	231094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.63110309230711	12.064237049419452	13.533138176360557	21.771521681912887
2	7.5926386570804985	21.507293940172037	41.517685197072794	29.382382205674666
3	22.091373980102595	32.223842778815424	26.671909512756365	19.012873728325612
4	13.416053356019809	13.037633329855455	31.525438012151174	42.02087530197357
5	18.41924178013349	31.72791233747344	27.54299769087404	22.309848191519027
6	39.00139069219547	26.06353837848902	13.362083670303605	21.572987259011907
7	26.611637586748067	32.74792185950927	21.58556801168816	19.0548725420545
8	25.78174702724399	34.96101550348703	25.41327580151523	13.843961667753751
9	29.633888169842177	23.752219847695873	13.01307327253208	33.60081870992987
10	12.913581733180843	25.256726659844702	29.289110937217842	32.540580669756615
11	29.24345902658368	27.828905411187264	16.661419356922007	26.266216205307046
12	27.690371556552307	22.086065267943038	24.333192829037195	25.890370346467463
13	32.81648536661592	19.398735772398705	28.29853514935831	19.486243711627065
14	36.25020412853747	17.85222505146055	21.87995830737625	24.01761251262573
15	38.30384004258649	15.23676633086794	27.012775945089246	19.44661768145633
16	29.209512539788452	18.792850072874103	35.09991714728632	16.89772024005112
17	24.987400362063195	29.743410893344567	29.56318567229652	15.706003072295715
18	27.398522311483838	15.841707909564656	42.36369684202038	14.396072936931127
19	23.395486878397342	37.0479619981612	17.371203445328	22.185347678113455
20	16.30216964433316	31.967880715930214	30.63488607452029	21.09506356521634
21	17.586396691915475	36.78248053919681	20.835811506924436	24.795311261963278
22	16.616909204895247	33.69564560054669	15.168735246627008	34.51870994793105
23	23.193264774131634	26.10847568339116	15.76068658660253	34.93757295587467
24	15.411935683488911	30.79665960504647	17.571541737803585	36.21986297366103
25	26.919673363744668	30.76899817975476	16.39006867746988	25.92125977903069
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	147.0
2	213.0
3	279.0
4	279.0
5	279.0
6	699.5
7	1120.0
8	1120.0
9	1120.0
10	1131.5
11	1143.0
12	1143.0
13	1143.0
14	1319.5
15	1496.0
16	1496.0
17	1496.0
18	2271.0
19	3046.0
20	3046.0
21	3046.0
22	3432.0
23	3818.0
24	3818.0
25	3818.0
26	5028.0
27	6238.0
28	6238.0
29	6238.0
30	7618.5
31	8999.0
32	8999.0
33	8999.0
34	10102.0
35	11205.0
36	11205.0
37	11205.0
38	12820.0
39	14435.0
40	14435.0
41	14435.0
42	19708.5
43	24982.0
44	24982.0
45	24982.0
46	68020.5
47	111059.0
48	111059.0
49	111059.0
50	172474.5
51	233890.0
52	233890.0
53	233890.0
54	139202.5
55	44515.0
56	44515.0
57	44515.0
58	28373.0
59	12231.0
60	12231.0
61	12231.0
62	10000.0
63	7769.0
64	7769.0
65	7769.0
66	6483.5
67	5198.0
68	5198.0
69	5198.0
70	4075.0
71	2952.0
72	2952.0
73	2952.0
74	2392.5
75	1833.0
76	1833.0
77	1833.0
78	1510.5
79	1188.0
80	1188.0
81	1188.0
82	906.5
83	625.0
84	625.0
85	625.0
86	468.0
87	311.0
88	311.0
89	311.0
90	206.5
91	102.0
92	102.0
93	102.0
94	58.5
95	15.0
96	15.0
97	15.0
98	8.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17609412010100342
2	0.020256840694990143
3	0.028078789082164553
4	0.04191762084408851
5	0.028078789082164553
6	0.05695982928096238
7	0.06317727543487026
8	0.06458121488897847
9	0.0505418203478962
10	0.04151649528577188
11	0.6227474292865781
12	0.5543555215935916
13	0.5298868625362768
14	0.518655346903411
15	0.5344998064569181
16	0.5086272079454951
17	0.5116356496328699
18	0.5118362124120281
19	0.525675044173952
20	0.5156469052160362
21	0.5214632258116274
22	0.5062204545955952
23	0.5170508446701444
24	0.4990001945458958
25	0.5034125756873788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	498597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.624803115462388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.86090248494983	18.121138152989303
2	6.1865651802661095	2.5519337760792715
3	1.6968360254165191	1.0499072683031831
4	0.8343383041477261	0.688322530189425
5	0.5614188684509923	0.5789576813553697
6	0.4075653914788321	0.5043573573556358
7	0.2976813662704082	0.4297733699328311
8	0.1987871177494479	0.3279956132378081
9	0.1957903138912274	0.3634323008329031
>10	1.3935075775650592	5.656275673813867
>50	0.15783096575091893	2.2572359684401206
>100	0.12386734020958193	5.878373188347346
>500	0.028968974726434484	4.4798593371451245
>1k	0.04295399700816147	18.464791821787962
>5k	0.005993580977882996	9.32582932744086
>10k+	0.006992511140863497	29.32181663274899
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	40015	8.025519608020105	No Hit
CTTCCGTACTCTGCGTTGATACCAC	24220	4.8576305112144675	No Hit
GAGTACGGAAGCAGTGGTATCAACG	20206	4.0525715156729785	No Hit
TCCGTACTCTGCGTTGATACCACTG	18771	3.7647639275807916	No Hit
GCTTCCGTACTCTGCGTTGATACCA	17698	3.5495600655439166	No Hit
GTACATGGGAAGCAGTGGTATCAAC	11178	2.241890745431681	No Hit
CCCATGTACTCTGCGTTGATACCAC	10232	2.0521583563479124	No Hit
GTACATGGAAGCAGTGGTATCAACG	9504	1.9061486531206566	No Hit
GCGTTGATACCACTGCTTCCGTACT	9022	1.8094773935663473	No Hit
GTATCAACGCAGAGTACGGAAGCAG	8364	1.6775070848801736	No Hit
TCCATGTACTCTGCGTTGATACCAC	6902	1.3842843017507125	No Hit
TATCAACGCAGAGTACGGAAGCAGT	5900	1.1833203970340778	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5573	1.1177363682493076	No Hit
CAGTGGTATCAACGCAGAGTACGGA	4114	0.8251152734573213	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3931	0.7884122848713491	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3818	0.765748690826459	No Hit
ATACCACTGCTTCCGTACTCTGCGT	3809	0.7639436258140342	No Hit
CATGTACTCTGCGTTGATACCACTG	3800	0.7621385608016092	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3601	0.722226567749104	No Hit
GTACGGGAAGCAGTGGTATCAACGC	3250	0.6518290322645343	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2994	0.6004849608000048	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2972	0.5960725796585218	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2711	0.5437256942982007	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2679	0.5373076853651346	No Hit
GTACTCTGCGTTGATACCACTGCTT	2643	0.5300874253154352	No Hit
GTATCAACGCAGAGTACATGGGAAG	2520	0.505418203478962	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2282	0.45768426203928225	No Hit
GCGTTGATACCACTGCTTCCCATGT	2276	0.4564808853643323	No Hit
GTTGATACCACTGCTTCCGTACTCT	2086	0.4183739573242518	No Hit
GATACCACTGCTTCCGTACTCTGCG	2069	0.41496439007856045	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2023	0.4057385022372778	No Hit
CCCGTACTCTGCGTTGATACCACTG	1989	0.3989193677458949	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1942	0.38949291712545403	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1935	0.3880889776713458	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1768	0.35459499355190666	No Hit
TATCAACGCAGAGTACATGGGAAGC	1759	0.35278992853948177	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1756	0.35218824020200684	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1749	0.35078430074789857	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1645	0.32992577171543347	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1616	0.3241094511198423	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1595	0.3198976327575176	No Hit
CAGTGGTATCAACGCAGAGTACATG	1486	0.2980362898292609	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1443	0.28941209032545323	No Hit
GCGTTGATACCACTGCTTCCATGTA	1393	0.27938395136753735	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1354	0.27156200298036287	No Hit
GTATCAACGCAGAGTACATGGAAGC	1337	0.2681524357346715	No Hit
CCATGTACTCTGCGTTGATACCACT	1221	0.24488715335230657	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1216	0.24388433945651497	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1171	0.23485901439439066	No Hit
CCCCATGTACTCTGCGTTGATACCA	1141	0.22884213101964113	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1141	0.22884213101964113	No Hit
GCCCATGTACTCTGCGTTGATACCA	1128	0.226234814890583	No Hit
TGATACCACTGCTTCCGTACTCTGC	1106	0.22182243374909996	No Hit
GGTATCAACGCAGAGTACATGGGAA	1060	0.21259654590781735	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1050	0.21059091811623415	No Hit
GCTTCCCGTACTCTGCGTTGATACC	1044	0.20938754144128424	No Hit
GTACAAGCAGTGGTATCAACGCAGA	988	0.1981560258084184	No Hit
GCGTTGATACCACTGCTTCCCGTAC	970	0.1945458957835687	No Hit
TATCAACGCAGAGTACATGGAAGCA	943	0.1891307007462941	No Hit
TACCACTGCTTCCGTACTCTGCGTT	930	0.18652338461723597	No Hit
CTGCGTTGATACCACTGCTTCCGTA	905	0.181509315138278	No Hit
AAAGTACTCTGCGTTGATACCACTG	887	0.17789918511342828	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	849	0.1702777995054122	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	847	0.16987667394709555	No Hit
TCCCATGTACTCTGCGTTGATACCA	845	0.1694755483887789	No Hit
ATACCACTGCTTCCCATGTACTCTG	820	0.16446147890982094	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	806	0.1616536000016045	No Hit
TACGGAAGCAGTGGTATCAACGCAG	774	0.15523559106853832	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	767	0.1538316516144301	No Hit
CATGGGAAGCAGTGGTATCAACGCA	755	0.15142489826453026	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	747	0.1498203960312637	No Hit
CTCTGCGTTGATACCACTGCTTCCG	729	0.14621026600641399	No Hit
TATCAACGCAGAGTACGGGAAGCAG	726	0.14560857766893906	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	726	0.14560857766893906	No Hit
TACCACTGCTTCCCATGTACTCTGC	716	0.14360294987735586	No Hit
GTATCAACGCAGAGTACTTTTTTTT	695	0.13939113151503119	No Hit
GTGGTATCAACGCAGAGTACATGGG	658	0.1319703086861734	No Hit
GCAGTGGTATCAACGCAGAGTACAT	651	0.1305663692320652	No Hit
CTTCCCATGTACTCTGCGTTGATAC	647	0.1297641181154319	No Hit
ATACCACTGCTTCCATGTACTCTGC	628	0.12595342531142387	No Hit
GGTATCAACGCAGAGTACATGGAAG	590	0.11833203970340776	No Hit
GCTTGTACTCTGCGTTGATACCACT	564	0.1131174074452915	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	546	0.10950727742044176	No Hit
ACATGGGAAGCAGTGGTATCAACGC	527	0.10569658461643372	No Hit
GTTGATACCACTGCTTCCCATGTAC	508	0.10188589181242566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.0056277915831824E-4	0.0
12	0.0	0.0	0.0	0.0010028138957915913	0.0
13	0.0	0.0	0.0	0.001805065012424864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAGA	25	0.006144024	18.93654	4
TCCGGAG	40	0.0053907563	14.202405	3
AAAAAGT	215	0.0	14.110727	1
AAAAGTA	220	0.0	13.772029	2
GTACTAT	50	0.0014837486	13.315744	6
ACGAAGC	50	0.0015352702	13.255578	3
CCCCGTA	115	3.947207E-10	13.173245	2
CTTCGTA	80	2.0678563E-6	13.018871	2
GCCCATG	130	2.0372681E-10	12.397767	1
GCTTCGT	85	4.018684E-6	12.269071	1
CTGTTCT	55	0.0030363551	12.105223	9
TACGAAG	55	0.0031411317	12.050525	2
TACGAAA	55	0.0031411317	12.050525	2
CGAAGCA	55	0.0031411317	12.050525	4
AGCCCGT	65	8.144743E-4	11.668487	1
GTACGAA	115	7.2313924E-8	11.541656	1
CCCCATG	215	0.0	11.464966	1
TGGGAGG	85	5.2421303E-5	11.189703	6
TCGTACT	115	9.1550646E-7	10.703261	4
AGTACAA	160	5.711627E-10	10.651804	2
>>END_MODULE